MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Mutations to a glycine loop in the catalytic site of human Lon changes its protease, peptidase and ATPase activities. Mutations to a glycine loop in the catalytic site of human Lon changes its protease, peptidase and ATPase activities. FEBS J. (1742-464X) 2014 imex curation Ambro L., Pevala V., Ondrovičová G., Bellová J., Kunová N., Kutejová E., Bauer J. evakutej@hotmail.com; jacob.bauer@savba.sk 10-Mar-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie evakutej@hotmail.com; jacob.bauer@savba.sk Accepted 2014-FEB-03 by LUANA 2014 imex curation Mutations to a glycine loop in the catalytic site of human Lon changes its protease, peptidase and ATPase activities. Mutations to a glycine loop in the catalytic site of human Lon changes its protease, peptidase and ATPase activities. FEBS J. (1742-464X) 2014 imex curation Ambro L., Pevala V., Ondrovičová G., Bellová J., Kunová N., Kutejová E., Bauer J. evakutej@hotmail.com; jacob.bauer@savba.sk 10-Mar-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro protease assay protease assay weight by staining molecular weight estimation by staining weight by staining evakutej@hotmail.com; jacob.bauer@savba.sk Accepted 2014-FEB-03 by LUANA 2014 imex curation casb_bovin Beta-casein CSN2 protein protein bovin Bos taurus (Bovine) Bovine MKVLILACLVALALARELEELNVPGEIVESLSSSEESITRINKKIEKFQSEEQQQTEDELQDKIHPFAQTQSLVYPFPGPIPNSLPQNIPPLTQTPVVVPPFLQPEVMGVSKVKEAMAPKHKEMPFPKYPVEPFTESQSLTLTDVENLHLPLPLLQSWMHQPHQPLPPTVMFPPQSVLSLSQSKVLPVPQKAVPYPQRDMPIQAFLLYQEPVLGPVRGPFPIIV DIP protein P02666 original sequence version: 88 DIP protein P02666 original sequence version: 101 F0BBDD8148A238AE lonm_human Lon protease homolog, mitochondrial LONHs PRSS15 LONP1 Lon protease-like protein Mitochondrial ATP-dependent protease Lon Serine protease 15 protein protein human Homo sapiens Human MAASTGYVRLWGAARCWVLRRPMLAAAGGRVPTAAGAWLLRGQRTCDASPPWALWGRGPAIGGQWRGFWEASSRGGGAFSGGEDASEGGAEEGAGGAGGSAGAGEGPVITALTPMTIPDVFPHLPLIAITRNPVFPRFIKIIEVKNKKLVELLRRKVRLAQPYVGVFLKRDDSNESDVVESLDEIYHTGTFAQIHEMQDLGDKLRMIVMGHRRVHISRQLEVEPEEPEAENKHKPRRKSKRGKKEAEDELSARHPAELAMEPTPELPAEVLMVEVENVVHEDFQVTEEVKALTAEIVKTIRDIIALNPLYRESVLQMMQAGQRVVDNPIYLSDMGAALTGAESHELQDVLEETNIPKRLYKALSLLKKEFELSKLQQRLGREVEEKIKQTHRKYLLQEQLKIIKKELGLEKDDKDAIEEKFRERLKELVVPKHVMDVVDEELSKLGLLDNHSSEFNVTRNYLDWLTSIPWGKYSNENLDLARAQAVLEEDHYGMEDVKKRILEFIAVSQLRGSTQGKILCFYGPPGVGKTSIARSIARALNREYFRFSVGGMTDVAEIKGHRRTYVGAMPGKIIQCLKKTKTENPLILIDEVDKIGRGYQGDPSSALLELLDPEQNANFLDHYLDVPVDLSKVLFICTANVTDTIPEPLRDRMEMINVSGYVAQEKLAIAERYLVPQARALCGLDESKAKLSSDVLTLLIKQYCRESGVRNLQKQVEKVLRKSAYKIVSGEAESVEVTPENLQDFVGKPVFTVERMYDVTPPGVVMGLAWTAMGGSTLFVETSLRRPQDKDAKGDKDGSLEVTGQLGEVMKESARIAYTFARAFLMQHAPANDYLVTSHIHLHVPEGATPKDGPSAGCTIVTALLSLAMGRPVRQNLAMTGEVSLTGKILPVGGIKEKTIAAKRAGVTCIVLPAENKKDFYDLAAFITEGLEVHFVEHYREIFDIAFPDEQAEALAVER B5E03D9C27C220FF lonp1-lonp1 1 hLon 4 weight by comassie molecular weight estimation by coomasie staining weight by comassie weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined hLon 4 weight by comassie molecular weight estimation by coomasie staining weight by comassie weight by comassie molecular weight estimation by coomasie staining weight by comassie unspecified role unspecified role neutral component neutral component purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction sf13 All mutants showed the same pattern of oligomerization as wild-type hLon by both gel-filtration and glutaraldehyde cross-linking (supplementary Figure S13). These residues, therefore, do not appear to be important for proper oligomerization. Correct oligomerization seems to be important for the proper functioning of Lon’s various activities, so verification that all mutants have the same oligomeric form as the wild-type allows us to directly compare their various activities and to look for the source of their differences only in their local effects csn2-lonp1 2 beta casein 3 weight by staining molecular weight estimation by staining weight by staining weight by staining molecular weight estimation by staining weight by staining enzyme target enzyme target substrate substrate neutral component neutral component purified purified fitc labelled fitc labelled fluorescein isothiocyanate label fluorescein isothiocyanate labbeled FITC labelled FITC labelled fluorescein isothiocyanate labbeled fitc labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined hLonP 4 predetermined predetermined participant predetermined weight by staining molecular weight estimation by staining weight by staining enzyme enzyme neutral component neutral component purified purified P36776:p.Gly894Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain G P Wed May 02 18:20:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly894pro' Tue Jun 30 18:48:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly894Pro' cleavage cleavage reaction cleavage f3 t1 In contrast to the ATPase activity, the protease activity could be classified into only two general groups: either a mutant possessed ≥75% of the wild-type activity (G893A, G894A, G894S, G893A-G894A, W770A), or it possessed little to no activity (G894P, G893A-G894P, W770P; G893P and G893P-G894A were inactive) csn2-lonp1-1 2 beta casein 3 weight by staining molecular weight estimation by staining weight by staining weight by staining molecular weight estimation by staining weight by staining enzyme target enzyme target substrate substrate neutral component neutral component purified purified fitc labelled fitc labelled fluorescein isothiocyanate label fluorescein isothiocyanate labbeled FITC labelled FITC labelled fluorescein isothiocyanate labbeled fitc labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined hLonP 4 predetermined predetermined participant predetermined weight by staining molecular weight estimation by staining weight by staining enzyme enzyme neutral component neutral component purified purified P36776:p.Gly893_Gly894delinsAlaPro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain GG AP Thu Jul 07 12:17:25 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was gly894pro_gly893ala Fri Jul 22 10:49:35 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'gly893ala,gly894pro' Wed May 02 17:26:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly_gly893-894ala_pro' Tue Jun 30 18:35:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly893_Gly894delinsAlaPro' cleavage cleavage reaction cleavage f3 t1 In contrast to the ATPase activity, the protease activity could be classified into only two general groups: either a mutant possessed ≥75% of the wild-type activity (G893A, G894A, G894S, G893A-G894A, W770A), or it possessed little to no activity (G894P, G893A-G894P, W770P; G893P and G893P-G894A were inactive) csn2-lonp1-2 2 beta casein 3 weight by staining molecular weight estimation by staining weight by staining weight by staining molecular weight estimation by staining weight by staining enzyme target enzyme target substrate substrate neutral component neutral component purified purified fitc labelled fitc labelled fluorescein isothiocyanate label fluorescein isothiocyanate labbeled FITC labelled FITC labelled fluorescein isothiocyanate labbeled fitc labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined hLonP 4 predetermined predetermined participant predetermined weight by staining molecular weight estimation by staining weight by staining enzyme enzyme neutral component neutral component purified purified P36776:p.Trp770Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain W P Wed May 02 14:45:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp770pro' Tue Jun 30 17:24:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp770Pro' cleavage cleavage reaction cleavage f3 t1 In contrast to the ATPase activity, the protease activity could be classified into only two general groups: either a mutant possessed ≥75% of the wild-type activity (G893A, G894A, G894S, G893A-G894A, W770A), or it possessed little to no activity (G894P, G893A-G894P, W770P; G893P and G893P-G894A were inactive) csn2-lonp1-3 2 hLonP 4 predetermined predetermined participant predetermined weight by staining molecular weight estimation by staining weight by staining enzyme enzyme neutral component neutral component purified purified P36776:p.Gly893Pro mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain G P Wed May 02 18:01:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly893pro' Tue Jun 30 16:00:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly893Pro' beta casein 3 weight by staining molecular weight estimation by staining weight by staining weight by staining molecular weight estimation by staining weight by staining enzyme target enzyme target substrate substrate neutral component neutral component purified purified fitc labelled fitc labelled fluorescein isothiocyanate label fluorescein isothiocyanate labbeled FITC labelled FITC labelled fluorescein isothiocyanate labbeled fitc labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cleavage cleavage reaction cleavage f3 t1 In contrast to the ATPase activity, the protease activity could be classified into only two general groups: either a mutant possessed ≥75% of the wild-type activity (G893A, G894A, G894S, G893A-G894A, W770A), or it possessed little to no activity (G894P, G893A-G894P, W770P; G893P and G893P-G894A were inactive) csn2-lonp1-4 2 hLonP 4 predetermined predetermined participant predetermined weight by staining molecular weight estimation by staining weight by staining enzyme enzyme neutral component neutral component purified purified P36776:p.Gly893_Gly894delinsProAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain GG PA Fri Jul 22 11:00:57 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'gly893pro,gly894ala' Wed May 02 15:55:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly_gly893-894pro_ala' Tue Jun 30 20:05:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly893_Gly894delinsProAla' beta casein 3 weight by staining molecular weight estimation by staining weight by staining weight by staining molecular weight estimation by staining weight by staining enzyme target enzyme target substrate substrate neutral component neutral component purified purified fitc labelled fitc labelled fluorescein isothiocyanate label fluorescein isothiocyanate labbeled FITC labelled FITC labelled fluorescein isothiocyanate labbeled fitc labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cleavage cleavage reaction cleavage f3 t1 In contrast to the ATPase activity, the protease activity could be classified into only two general groups: either a mutant possessed ≥75% of the wild-type activity (G893A, G894A, G894S, G893A-G894A, W770A), or it possessed little to no activity (G894P, G893A-G894P, W770P; G893P and G893P-G894A were inactive) csn2-lonp1-5 2 hLonP 4 predetermined predetermined participant predetermined weight by staining molecular weight estimation by staining weight by staining enzyme enzyme neutral component neutral component purified purified P36776:p.Lys529Arg mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain K R Wed May 02 18:43:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys529arg' Tue Jun 30 19:48:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys529Arg' beta casein 3 weight by staining molecular weight estimation by staining weight by staining weight by staining molecular weight estimation by staining weight by staining enzyme target enzyme target substrate substrate neutral component neutral component purified purified fitc labelled fitc labelled fluorescein isothiocyanate label fluorescein isothiocyanate labbeled FITC labelled FITC labelled fluorescein isothiocyanate labbeled fitc labelled undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cleavage cleavage reaction cleavage f3 t1 sf23 In contrast to the ATPase activity, the protease activity could be classified into only two general groups: either a mutant possessed ≥75% of the wild-type activity (G893A, G894A, G894S, G893A-G894A, W770A), or it possessed little to no activity (G894P, G893A-G894P, W770P; G893P and G893P-G894A were inactive)