IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ LRRK2 regulates synaptogenesis and dopamine receptor activation through modulation of PKA activity LRRK2 regulates synaptogenesis and dopamine receptor activation through modulation of PKA activity Nature Neuroscience 2014 imex curation Origin of LRP8 (APOER2) taken from work of the contributing scientist (PMID: 8626535) Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. I was a little confused by Supp Figs 6 +7 - so when in doubt (much of the time) I have just entered "supp fig 6" or similar... The authors have stated that PKAR2a, R1a, RIb, and the catalytic subunit (type a) were from mouse and RI2b was from Rat. GST-PKAcalpha was the human recombinant protein. Figure 7A/B uses 293 cells, and the LRRK2 "G2835R" mutation is really a "G2385R" mutation. caih@mail.nih.gov, loukia.parisiadou@northwestern.edu 10-Mar-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Origin of LRP8 (APOER2) taken from work of the contributing scientist (PMID: 8626535) Parkinsons - Interactions investigated in the context of Parkinsons disease Parkinsons - Interactions investigated in the context of Parkinsons disease I was a little confused by Supp Figs 6 +7 - so when in doubt (much of the time) I have just entered "supp fig 6" or similar... The authors have stated that PKAR2a, R1a, RIb, and the catalytic subunit (type a) were from mouse and RI2b was from Rat. GST-PKAcalpha was the human recombinant protein. Figure 7A/B uses 293 cells, and the LRRK2 "G2835R" mutation is really a "G2385R" mutation. caih@mail.nih.gov, loukia.parisiadou@northwestern.edu Accepted 2014-FEB-26 by ORCHARD Nature Neuroscience 2014 Only protein-protein interactions imex curation LRRK2 regulates synaptogenesis and dopamine receptor activation through modulation of PKA activity LRRK2 regulates synaptogenesis and dopamine receptor activation through modulation of PKA activity Nature Neuroscience 2014 imex curation Origin of LRP8 (APOER2) taken from work of the contributing scientist (PMID: 8626535) Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. I was a little confused by Supp Figs 6 +7 - so when in doubt (much of the time) I have just entered "supp fig 6" or similar... The authors have stated that PKAR2a, R1a, RIb, and the catalytic subunit (type a) were from mouse and RI2b was from Rat. GST-PKAcalpha was the human recombinant protein. Figure 7A/B uses 293 cells, and the LRRK2 "G2835R" mutation is really a "G2385R" mutation. caih@mail.nih.gov, loukia.parisiadou@northwestern.edu 10-Mar-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Origin of LRP8 (APOER2) taken from work of the contributing scientist (PMID: 8626535) Parkinsons - Interactions investigated in the context of Parkinsons disease Parkinsons - Interactions investigated in the context of Parkinsons disease I was a little confused by Supp Figs 6 +7 - so when in doubt (much of the time) I have just entered "supp fig 6" or similar... The authors have stated that PKAR2a, R1a, RIb, and the catalytic subunit (type a) were from mouse and RI2b was from Rat. GST-PKAcalpha was the human recombinant protein. Figure 7A/B uses 293 cells, and the LRRK2 "G2835R" mutation is really a "G2385R" mutation. caih@mail.nih.gov, loukia.parisiadou@northwestern.edu Accepted 2014-FEB-26 by ORCHARD Nature Neuroscience 2014 Only protein-protein interactions imex curation LRRK2 regulates synaptogenesis and dopamine receptor activation through modulation of PKA activity LRRK2 regulates synaptogenesis and dopamine receptor activation through modulation of PKA activity Nature Neuroscience 2014 imex curation Origin of LRP8 (APOER2) taken from work of the contributing scientist (PMID: 8626535) Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. I was a little confused by Supp Figs 6 +7 - so when in doubt (much of the time) I have just entered "supp fig 6" or similar... The authors have stated that PKAR2a, R1a, RIb, and the catalytic subunit (type a) were from mouse and RI2b was from Rat. GST-PKAcalpha was the human recombinant protein. Figure 7A/B uses 293 cells, and the LRRK2 "G2835R" mutation is really a "G2385R" mutation. caih@mail.nih.gov, loukia.parisiadou@northwestern.edu 10-Mar-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-striatum Mus musculus, striatum striatum striatum anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Origin of LRP8 (APOER2) taken from work of the contributing scientist (PMID: 8626535) Parkinsons - Interactions investigated in the context of Parkinsons disease Parkinsons - Interactions investigated in the context of Parkinsons disease I was a little confused by Supp Figs 6 +7 - so when in doubt (much of the time) I have just entered "supp fig 6" or similar... The authors have stated that PKAR2a, R1a, RIb, and the catalytic subunit (type a) were from mouse and RI2b was from Rat. GST-PKAcalpha was the human recombinant protein. Figure 7A/B uses 293 cells, and the LRRK2 "G2835R" mutation is really a "G2385R" mutation. caih@mail.nih.gov, loukia.parisiadou@northwestern.edu Accepted 2014-FEB-26 by ORCHARD Nature Neuroscience 2014 Only protein-protein interactions imex curation LRRK2 regulates synaptogenesis and dopamine receptor activation through modulation of PKA activity LRRK2 regulates synaptogenesis and dopamine receptor activation through modulation of PKA activity Nature Neuroscience 2014 imex curation Origin of LRP8 (APOER2) taken from work of the contributing scientist (PMID: 8626535) Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. I was a little confused by Supp Figs 6 +7 - so when in doubt (much of the time) I have just entered "supp fig 6" or similar... The authors have stated that PKAR2a, R1a, RIb, and the catalytic subunit (type a) were from mouse and RI2b was from Rat. GST-PKAcalpha was the human recombinant protein. Figure 7A/B uses 293 cells, and the LRRK2 "G2835R" mutation is really a "G2385R" mutation. caih@mail.nih.gov, loukia.parisiadou@northwestern.edu 10-Mar-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-brain_hippocampal_neuron Mus musculus, brain, hippocampal neurons brain-hippocampus_neuron Hippocampal neuron confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Origin of LRP8 (APOER2) taken from work of the contributing scientist (PMID: 8626535) Parkinsons - Interactions investigated in the context of Parkinsons disease Parkinsons - Interactions investigated in the context of Parkinsons disease I was a little confused by Supp Figs 6 +7 - so when in doubt (much of the time) I have just entered "supp fig 6" or similar... The authors have stated that PKAR2a, R1a, RIb, and the catalytic subunit (type a) were from mouse and RI2b was from Rat. GST-PKAcalpha was the human recombinant protein. Figure 7A/B uses 293 cells, and the LRRK2 "G2835R" mutation is really a "G2385R" mutation. caih@mail.nih.gov, loukia.parisiadou@northwestern.edu Accepted 2014-FEB-26 by ORCHARD Nature Neuroscience 2014 Only protein-protein interactions imex curation LRRK2 regulates synaptogenesis and dopamine receptor activation through modulation of PKA activity LRRK2 regulates synaptogenesis and dopamine receptor activation through modulation of PKA activity Nature Neuroscience 2014 imex curation Origin of LRP8 (APOER2) taken from work of the contributing scientist (PMID: 8626535) Parkinsons - Interactions investigated in the context of Parkinsons disease Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. I was a little confused by Supp Figs 6 +7 - so when in doubt (much of the time) I have just entered "supp fig 6" or similar... The authors have stated that PKAR2a, R1a, RIb, and the catalytic subunit (type a) were from mouse and RI2b was from Rat. GST-PKAcalpha was the human recombinant protein. Figure 7A/B uses 293 cells, and the LRRK2 "G2835R" mutation is really a "G2385R" mutation. caih@mail.nih.gov, loukia.parisiadou@northwestern.edu 10-Mar-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-forebrain Mus musculus, forebrain brain_forebrain anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Origin of LRP8 (APOER2) taken from work of the contributing scientist (PMID: 8626535) Parkinsons - Interactions investigated in the context of Parkinsons disease Parkinsons - Interactions investigated in the context of Parkinsons disease I was a little confused by Supp Figs 6 +7 - so when in doubt (much of the time) I have just entered "supp fig 6" or similar... The authors have stated that PKAR2a, R1a, RIb, and the catalytic subunit (type a) were from mouse and RI2b was from Rat. GST-PKAcalpha was the human recombinant protein. Figure 7A/B uses 293 cells, and the LRRK2 "G2835R" mutation is really a "G2385R" mutation. caih@mail.nih.gov, loukia.parisiadou@northwestern.edu Accepted 2014-FEB-26 by ORCHARD Nature Neuroscience 2014 Only protein-protein interactions imex curation kap3_rat cAMP-dependent protein kinase type II-beta regulatory subunit Prkar2b protein protein rat Rattus norvegicus (Rat) Rat MSIEIPAGLTELLQGFTVEVLRHQPADLLEFALQHFTRLQQENERKGAARFGHEGRTWGDAGAAAGGGTPSKGVNFAEEPMRSDSENGEEEEAAEAGAFNAPVINRFTRRASVCAEAYNPDEEEDDAESRIIHPKTDDQRNRLQEACKDILLFKNLDPEQMSQVLDAMFEKLVKEGEHVIDQGDDGDNFYVIDRGTFDIYVKCDGVGRCVGNYDNRGSFGELALMYNTPRAATITATSPGALWGLDRVTFRRIIVKNNAKKRKMYESFIESLPFLKSLEVSERLKVVDVIGTKVYNDGEQIIAQGDSADSFFIVESGEVRITMKRKGKSDIEENGAVEIARCLRGQYFGELALVTNKPRAASAHAIGTVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQLVALFGTNMDIVEPTA DIP protein P12369 original sequence version: 133 8BC984E325FA3612 kap3_mouse cAMP-dependent protein kinase type II-beta regulatory subunit Prkar2b protein protein mouse Mus musculus Mouse MSIEIPAGLTELLQGFTVEVLRHQPADLLEFALQHFTRLQQENERKGAARFGHEGRTWGDAGAAAGGGIPSKGVNFAEEPMRSDSENGEEEEAAEAGAFNAPVINRFTRRASVCAEAYNPDEEEDDAESRIIHPKTDDQRNRLQEACKDILLFKNLDPEQMSQVLDAMFEKLVKEGEHVIDQGDDGDNFYVIDRGTFDIYVKCDGVGRCVGNYDNRGSFGELALMYNTPRAATITATSPGALWGLDRVTFRRIIVKNNAKKRKMYESFIESLPFLKSLEVSERLKVVDVIGTKVYNDGEQIIAQGDLADSFFIVESGEVKITMKRKGKSEVEENGAVEIARCFRGQYFGELALVTNKPRAASAHAIGTVKCLAMDVQAFERLLGPCMEIMKRNIATYEEQLVALFGTNMDIVEPTA DIP protein P31324 original sequence version: 140 DIP protein P31324 original sequence version: 139 DIP protein P31324 original sequence version: 144 DIP protein P31324 original sequence version: 98 24196C3037789827 lrrk2_mouse Leucine-rich repeat serine/threonine-protein kinase 2 Lrrk2 protein protein mouse Mus musculus Mouse MASGACQGCEEEEEEEALKKLIVRLNNVQEGKQIETLLQLLEDMLVFTYSDRASKLFEDKNFHVPLLIVLDSYMRVASVQQAGWSLLCKLIEVCPGTLQSLIGPQDIGNDWEVLGIHRLILKMLTVHHANVNLSIVGLKALDLLLDSGKLTLLILDEECDIFLLIFDAMHRYSANDEVQKLGCKALHVLFERVSEEQLTEFVENKDYTILLSTFGSFRRDKEIVYHVLCCLHSLAVTCSNVEVLMSGNVRCYNLVVEAMKAFPTNENIQEVSCSLFQKLTLGNFFNILVLNEVHVFVVKAVRQYPENAALQISALSCLALLTETIFLNQDLEERSETQEQSEEEDSEKLFWLEPCYKALVRHRKDKHVQEAACWALNNLLMYQNSLHEKIGDEDGQFPAHREVMLSMLMHSSSKDVFQAAAHALSTLLEQNVNFRKILLAKGVYLNVLELMQKHAHAPEVAESGCKMLSHLFEGSNPSLDTMAAVVPKILTVMKAHGTSLSVQLEALRAILHFVVPGLLEESREDSQCRPNVLRKQCFRTDIHKLVLVALNRFIGNPGIQKCGLKVISSLAHLPDATETLSLQGAVDSVLHTLQMYPDDQEIQCLGLHLMGCLMTKKNFCIGTGHLLAKILASTLQRFKDVAEVQTTGLQTTLSILELSVSFSKLLVHYSFDVVIFHQMSSSVVEQKDEQFLNLCCKCFAKVAVDDELKNTMLERACDQNNSIMVECLLLLGADANQVKGATSLIYQVCEKESSPKLVELLLNGGCREQDVRKALTVSIQKGDSQVISLLLRKLALDLANNSICLGGFGIGKIDPSWLGPLFPDKSSNLRKQTNTGSVLARKVLRYQMRNTLQEGVASGSDGKFSEDALAKFGEWTFIPDSSMDSVFGQSDDLDSEGSESSFLVKRKSNSISVGEVYRDLALQRCSPNAQRHSNSLGPVFDHEDLLRRKRKILSSDESLRSSRLPSHMRQSDSSSSLASEREHITSLDLSANELKDIDALSQKCCLSSHLEHLTKLELHQNSLTSFPQQLCETLKCLIHLDLHSNKFTSFPSFVLKMPRITNLDASRNDIGPTVVLDPAMKCPSLKQLNLSYNQLSSIPENLAQVVEKLEQLLLEGNKISGICSPLSLKELKILNLSKNHIPSLPGDFLEACSKVESFSARMNFLAAMPALPSSITSLKLSQNSFTCIPEAIFSLPHLRSLDMSHNNIECLPGPAHWKSLNLRELIFSKNQISTLDFSENPHVWSRVEKLHLSHNKLKEIPPEIGCLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDGLHLNFDFKHVGCKAKDIIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKMKKPELGMQGATVGIDVRDWSIQIRGKRRKDLVLNVWDFAGREEFYSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACISKITKELLNKRGFPTIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQPVVGQLIPDCYVELEKIILSERKAVPTEFPVINRKHLLQLVNEHQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKVMAQILTVKVDGCLKHPKGIISRRDVEKFLSKKKRFPKNYMMQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPFMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVGSEVLDNRPESFLKITVPSCRKGCILLGRVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFNDGEEHQKILLDELMKKAEEGDLLINPDQPRLTIPISQIAPDLILADLPRNIMLNNDELEFEEAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTRTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYGIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLHDIWTTGSRIMEGLRFPNEFDELAIQGKLPDPVKEYGCAPWPMVEKLITKCLKENPQERPTSAQVFDILNSAELICLMRHILIPKNIIVECMVATNLNSKSATLWLGCGNTEKGQLSLFDLNTERYSYEEVADSRILCLALVHLAAEKESWVVCGTQSGALLVINVEEETKRHTLEKMTDSVTCLHCNSLAKQSKQSNFLLVGTADGNLMIFEDKAVKCKGAAPLKTLHIGDVSTPLMCLSESLNSSERHITWGGCGTKVFSFSNDFTIQKLIETKTNQLFSYAAFSDSNIIALAVDTALYIAKKNSPVVEVWDKKTEKLCELIDCVHFLKEVMVKLNKESKHQLSYSGRVKALCLQKNTALWIGTGGGHILLLDLSTRRVIRTIHNFCDSVRAMATAQLGSLKNVMLVLGYKRKSTEGIQEQKEIQSCLSIWDLNLPHEVQNLEKHIEVRTELADKMRKTSVE DIP protein Q5S006 original sequence version: 57 706E25C173E36F98 lrrk2_human Leucine-rich repeat serine/threonine-protein kinase 2 LRRK2 PARK8 Dardarin protein protein human Homo sapiens Human MASGSCQGCEEDEETLKKLIVRLNNVQEGKQIETLVQILEDLLVFTYSERASKLFQGKNIHVPLLIVLDSYMRVASVQQVGWSLLCKLIEVCPGTMQSLMGPQDVGNDWEVLGVHQLILKMLTVHNASVNLSVIGLKTLDLLLTSGKITLLILDEESDIFMLIFDAMHSFPANDEVQKLGCKALHVLFERVSEEQLTEFVENKDYMILLSALTNFKDEEEIVLHVLHCLHSLAIPCNNVEVLMSGNVRCYNIVVEAMKAFPMSERIQEVSCCLLHRLTLGNFFNILVLNEVHEFVVKAVQQYPENAALQISALSCLALLTETIFLNQDLEEKNENQENDDEGEEDKLFWLEACYKALTWHRKNKHVQEAACWALNNLLMYQNSLHEKIGDEDGHFPAHREVMLSMLMHSSSKEVFQASANALSTLLEQNVNFRKILLSKGIHLNVLELMQKHIHSPEVAESGCKMLNHLFEGSNTSLDIMAAVVPKILTVMKRHETSLPVQLEALRAILHFIVPGMPEESREDTEFHHKLNMVKKQCFKNDIHKLVLAALNRFIGNPGIQKCGLKVISSIVHFPDALEMLSLEGAMDSVLHTLQMYPDDQEIQCLGLSLIGYLITKKNVFIGTGHLLAKILVSSLYRFKDVAEIQTKGFQTILAILKLSASFSKLLVHHSFDLVIFHQMSSNIMEQKDQQFLNLCCKCFAKVAMDDYLKNVMLERACDQNNSIMVECLLLLGADANQAKEGSSLICQVCEKESSPKLVELLLNSGSREQDVRKALTISIGKGDSQIISLLLRRLALDVANNSICLGGFCIGKVEPSWLGPLFPDKTSNLRKQTNIASTLARMVIRYQMKSAVEEGTASGSDGNFSEDVLSKFDEWTFIPDSSMDSVFAQSDDLDSEGSEGSFLVKKKSNSISVGEFYRDAVLQRCSPNLQRHSNSLGPIFDHEDLLKRKRKILSSDDSLRSSKLQSHMRHSDSISSLASEREYITSLDLSANELRDIDALSQKCCISVHLEHLEKLELHQNALTSFPQQLCETLKSLTHLDLHSNKFTSFPSYLLKMSCIANLDVSRNDIGPSVVLDPTVKCPTLKQFNLSYNQLSFVPENLTDVVEKLEQLILEGNKISGICSPLRLKELKILNLSKNHISSLSENFLEACPKVESFSARMNFLAAMPFLPPSMTILKLSQNKFSCIPEAILNLPHLRSLDMSSNDIQYLPGPAHWKSLNLRELLFSHNQISILDLSEKAYLWSRVEKLHLSHNKLKEIPPEIGCLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDELHLNFDFKHIGCKAKDIIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKTKKSDLGMQSATVGIDVKDWPIQIRDKRKRDLVLNVWDFAGREEFYSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACMSKITKELLNKRGFPAIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQLVVGQLIPDCYVELEKIILSERKNVPIEFPVIDRKRLLQLVRENQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKIMAQILTVKVEGCPKHPKGIISRRDVEKFLSKKRKFPKNYMSQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPYMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVGSEVLDNHPESFLKITVPSCRKGCILLGQVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFNDGEEHQKILLDDLMKKAEEGDLLVNPDQPRLTIPISQIAPDLILADLPRNIMLNNDELEFEQAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTRTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYGIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLYDILTTGGRIVEGLKFPNEFDELEIQGKLPDPVKEYGCAPWPMVEKLIKQCLKENPQERPTSAQVFDILNSAELVCLTRRILLPKNVIVECMVATHHNSRNASIWLGCGHTDRGQLSFLDLNTEGYTSEEVADSRILCLALVHLPVEKESWIVSGTQSGTLLVINTEDGKKRHTLEKMTDSVTCLYCNSFSKQSKQKNFLLVGTADGKLAIFEDKTVKLKGAAPLKILNIGNVSTPLMCLSESTNSTERNVMWGGCGTKIFSFSNDFTIQKLIETRTSQLFSYAAFSDSNIITVVVDTALYIAKQNSPVVEVWDKKTEKLCGLIDCVHFLREVMVKENKESKHKMSYSGRVKTLCLQKNTALWIGTGGGHILLLDLSTRRLIRVIYNFCNSVRVMMTAQLGSLKNVMLVLGYNRKNTEGTQKQKEIQSCLTVWDINLPHEVQNLEKHIEVRKELAEKMRRTSVE DIP protein Q5S007 original sequence version: 36 DIP protein Q5S007 original sequence version: 68 DIP protein Q5S007 original sequence version: 64 26142A0CECBBC3F4 kapca_human cAMP-dependent protein kinase catalytic subunit alpha PRKACA PKACA protein protein human Homo sapiens Human MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWESPAQNTAHLDQFERIKTLGTGSFGRVMLVKHKETGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVPGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFSEF DIP protein P17612 original sequence version: 171 DIP protein P17612 original sequence version: 175 DIP protein P17612 original sequence version: 178 DIP protein P17612-1 original sequence version: 177 DIP protein P17612 original sequence version: 186 DIP protein P17612 original sequence version: 102 DIP protein P17612-1 original sequence version: 102 BF6D3ECD2614E5AB kapca_mouse cAMP-dependent protein kinase catalytic subunit alpha Prkaca Pkaca protein protein mouse Mus musculus Mouse MGNAAAAKKGSEQESVKEFLAKAKEDFLKKWETPSQNTAQLDQFDRIKTLGTGSFGRVMLVKHKESGNHYAMKILDKQKVVKLKQIEHTLNEKRILQAVNFPFLVKLEFSFKDNSNLYMVMEYVAGGEMFSHLRRIGRFSEPHARFYAAQIVLTFEYLHSLDLIYRDLKPENLLIDQQGYIQVTDFGFAKRVKGRTWTLCGTPEYLAPEIILSKGYNKAVDWWALGVLIYEMAAGYPPFFADQPIQIYEKIVSGKVRFPSHFSSDLKDLLRNLLQVDLTKRFGNLKNGVNDIKNHKWFATTDWIAIYQRKVEAPFIPKFKGPGDTSNFDDYEEEEIRVSINEKCGKEFTEF DIP protein P05132 original sequence version: 185 DIP protein P05132 original sequence version: 186 DIP protein P05132-1 original sequence version: 145 DIP protein P05132-1 original sequence version: 193 DIP protein P05132-1 original sequence version: 199 DIP protein P05132 original sequence version: 163 DIP protein P05132 original sequence version: 192 DIP protein P05132-1 original sequence version: 197 DIP protein P05132-1 original sequence version: 149 02F85D66EB21A1FA lrrk2-prkar2b-1 5 9 unspecified role unspecified role bait bait n_terminus ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 7 unspecified role unspecified role prey prey physical association physical association Figure 4A, (Supp Fig 6) lrrk2-prkar2b-2 1 9 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q5S007:p.Arg1441Cys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R C Wed May 02 19:40:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1441cys' Tue Jun 30 19:08:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg1441Cys' 6 unspecified role unspecified role prey prey region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 4B, 7C, Supp Fig 6, Supp fig 10B. lrrk2-prkar2b-3 3 8 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association Figure 4D, Supp fig6. prkar2b-lrrk2-1 3 11 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey association association Figure 4E, Supp fig 6 lrrk2-prkar2b-5 4 7 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization Figure 4F lrrk2-prkar2b-7 2 6 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined roc_domain sufficient to bind sufficient binding region sufficient to bind range range range range physical association physical association Figure 4H, Supp Fig 6. prkaca-prkar2b-1 4 11 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component colocalization colocalization Supp Figure 6F prkaca-lrrk2-1 1 10 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Supp Fig 6E, Supp fig 7C. prkaca-lrrk2-2 1 10 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp Fig 6E, Supp fig 7C. prkar2b-lrrk2-2 3 11 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association Figure 4E, Supp fig 6 -1 Interaction observed in LRRK2 -/- cells. lrrk2-prkar2b-8 2 9 unspecified role unspecified role bait bait Q5S007:p.Gly2385Arg mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain G R Wed May 02 14:33:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly2385arg' Tue Jun 30 18:10:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly2385Arg' region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q5S007:p.Arg1441Cys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R C Wed May 02 16:34:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1441cys' Tue Jun 30 15:57:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg1441Cys' 6 unspecified role unspecified role prey prey n_terminal_region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 4I and 7A/B, Supp fig 6 and 10. The LRRK2 "G2835R" mutation, as described in Figure 7B, is not correct as the LRRK2 molecule only has 2527 amino acids. The authors have confirmed this really is a "G2385R" mutation. ???cell lines for Figs Supp 10 and 7A/B