IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Hepatic RIG-I Predicts Survival and Interferon-α Therapeutic Response in Hepatocellular Carcinoma. Hepatic RIG-I Predicts Survival and Interferon-α Therapeutic Response in Hepatocellular Carcinoma. Cancer Cell (1535-6108) 2014 imex curation Hou J., Zhou Y., Zheng Y., Fan J., Zhou W., Ng I.O., Sun H., Qin L., Qiu S., Lee J.M., Lo C.M., Man K., Yang Y., Yang Y., Yang Y., Zhang Q., Zhu X., Li N., Wang Z., Ding G., Zhuang S.M., Zheng L., Luo X., Xie Y., Liang A., Wang Z., Zhang M., Xia Q., Liang T., Yu Y., Cao X. Cancer - Interactions investigated in the context of cancer Co-IP results from figures 5F and G have not been included as the origins of the constructs is not given in this publication; information from previous publications is conflicting, and sequence lengths of molecules involved does not allow unambiguous identification. The corresponding author did not reply to a request for information. Dephosphorylation assay results have not been included as STAT1 was not further purified after IP. ChIp results have not been included due to lack of antibody specificity to (methylated) Histones . caoxt@immunol.org 14-Apr-2014: Contacted by IntAct-Help. Only protein-protein interactions human-bel-7402 Human BEL-7402 Hepatoma cell line bel-7402 BEL-7402 Human Hepatoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hou J., Zhou Y., Zheng Y., Fan J., Zhou W., Ng I.O., Sun H., Qin L., Qiu S., Lee J.M., Lo C.M., Man K., Yang Y., Yang Y., Yang Y., Zhang Q., Zhu X., Li N., Wang Z., Ding G., Zhuang S.M., Zheng L., Luo X., Xie Y., Liang A., Wang Z., Zhang M., Xia Q., Liang T., Yu Y., Cao X. Cancer - Interactions investigated in the context of cancer Co-IP results from figures 5F and G have not been included as the origins of the constructs is not given in this publication; information from previous publications is conflicting, and sequence lengths of molecules involved does not allow unambiguous identification. The corresponding author did not reply to a request for information. caoxt@immunol.org Accepted 2014-APR-02 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation Hepatic RIG-I Predicts Survival and Interferon-α Therapeutic Response in Hepatocellular Carcinoma. Hepatic RIG-I Predicts Survival and Interferon-α Therapeutic Response in Hepatocellular Carcinoma. Cancer Cell (1535-6108) 2014 imex curation Hou J., Zhou Y., Zheng Y., Fan J., Zhou W., Ng I.O., Sun H., Qin L., Qiu S., Lee J.M., Lo C.M., Man K., Yang Y., Yang Y., Yang Y., Zhang Q., Zhu X., Li N., Wang Z., Ding G., Zhuang S.M., Zheng L., Luo X., Xie Y., Liang A., Wang Z., Zhang M., Xia Q., Liang T., Yu Y., Cao X. Cancer - Interactions investigated in the context of cancer Co-IP results from figures 5F and G have not been included as the origins of the constructs is not given in this publication; information from previous publications is conflicting, and sequence lengths of molecules involved does not allow unambiguous identification. The corresponding author did not reply to a request for information. Dephosphorylation assay results have not been included as STAT1 was not further purified after IP. ChIp results have not been included due to lack of antibody specificity to (methylated) Histones . caoxt@immunol.org 14-Apr-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-liver Mus musculus liver liver anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hou J., Zhou Y., Zheng Y., Fan J., Zhou W., Ng I.O., Sun H., Qin L., Qiu S., Lee J.M., Lo C.M., Man K., Yang Y., Yang Y., Yang Y., Zhang Q., Zhu X., Li N., Wang Z., Ding G., Zhuang S.M., Zheng L., Luo X., Xie Y., Liang A., Wang Z., Zhang M., Xia Q., Liang T., Yu Y., Cao X. Cancer - Interactions investigated in the context of cancer Co-IP results from figures 5F and G have not been included as the origins of the constructs is not given in this publication; information from previous publications is conflicting, and sequence lengths of molecules involved does not allow unambiguous identification. The corresponding author did not reply to a request for information. caoxt@immunol.org Accepted 2014-APR-02 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation Hepatic RIG-I Predicts Survival and Interferon-α Therapeutic Response in Hepatocellular Carcinoma. Hepatic RIG-I Predicts Survival and Interferon-α Therapeutic Response in Hepatocellular Carcinoma. Cancer Cell (1535-6108) 2014 imex curation Hou J., Zhou Y., Zheng Y., Fan J., Zhou W., Ng I.O., Sun H., Qin L., Qiu S., Lee J.M., Lo C.M., Man K., Yang Y., Yang Y., Yang Y., Zhang Q., Zhu X., Li N., Wang Z., Ding G., Zhuang S.M., Zheng L., Luo X., Xie Y., Liang A., Wang Z., Zhang M., Xia Q., Liang T., Yu Y., Cao X. Cancer - Interactions investigated in the context of cancer Co-IP results from figures 5F and G have not been included as the origins of the constructs is not given in this publication; information from previous publications is conflicting, and sequence lengths of molecules involved does not allow unambiguous identification. The corresponding author did not reply to a request for information. Dephosphorylation assay results have not been included as STAT1 was not further purified after IP. ChIp results have not been included due to lack of antibody specificity to (methylated) Histones . caoxt@immunol.org 14-Apr-2014: Contacted by IntAct-Help. Only protein-protein interactions human-smmc7721 Human SMMC7721 Hepatoma cell line smmc7721 SMMC7721 Hepatoma cell line This cell line originated in China prior to 1980. No PMID reference findable but:- Tong R et al., The establishment and primary biological characteristics of human hepatoma SMMC-7721 cell line.J 2nd Milit Med Univ, 1980;1(1):5-9. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Hou J., Zhou Y., Zheng Y., Fan J., Zhou W., Ng I.O., Sun H., Qin L., Qiu S., Lee J.M., Lo C.M., Man K., Yang Y., Yang Y., Yang Y., Zhang Q., Zhu X., Li N., Wang Z., Ding G., Zhuang S.M., Zheng L., Luo X., Xie Y., Liang A., Wang Z., Zhang M., Xia Q., Liang T., Yu Y., Cao X. Cancer - Interactions investigated in the context of cancer Co-IP results from figures 5F and G have not been included as the origins of the constructs is not given in this publication; information from previous publications is conflicting, and sequence lengths of molecules involved does not allow unambiguous identification. The corresponding author did not reply to a request for information. caoxt@immunol.org Accepted 2014-APR-02 by ORCHARD Cancer Cell (1535-6108) 2014 Only protein-protein interactions imex curation ptn6_mouse Tyrosine-protein phosphatase non-receptor type 6 Ptpn6 Hcp Hcph Ptp1C Protein-tyrosine phosphatase 1C Hematopoietic cell protein-tyrosine phosphatase 70Z-SHP SH-PTP1 PTPTY-42 protein protein mouse Mus musculus Mouse MVRWFHRDLSGPDAETLLKGRGVPGSFLARPSRKNQGDFSLSVRVDDQVTHIRIQNSGDFYDLYGGEKFATLTELVEYYTQQQGILQDRDGTIIHLKYPLNCSDPTSERWYHGHISGGQAESLLQAKGEPWTFLVRESLSQPGDFVLSVLNDQPKAGPGSPLRVTHIKVMCEGGRYTVGGSETFDSLTDLVEHFKKTGIEEASGAFVYLRQPYYATRVNAADIENRVLELNKKQESEDTAKAGFWEEFESLQKQEVKNLHQRLEGQRPENKSKNRYKNILPFDHSRVILQGRDSNIPGSDYINANYVKNQLLGPDENSKTYIASQGCLDATVNDFWQMAWQENTRVIVMTTREVEKGRNKCVPYWPEVGTQRVYGLYSVTNSREHDTAEYKLRTLQISPLDNGDLVREIWHYQYLSWPDHGVPSEPGGVLSFLDQINQRQESLPHAGPIIVHCSAGIGRTGTIIVIDMLMESISTKGLDCDIDIQKTIQMVRAQRSGMVQTEAQYKFIYVAIAQFIETTKKKLEIIQSQKGQESEYGNITYPPAVRSAHAKASRTSSKHKEEVYENVHSKSKKEEKVKKQRSADKEKNKGSLKRK DIP protein P29351 original sequence version: 154 DIP protein P29351 original sequence version: 102 DIP protein P29351 original sequence version: 117 DIP protein P29351 original sequence version: 164 DIP protein P29351 original sequence version: 134 CF17300D032638D2 stat1_mouse Signal transducer and activator of transcription 1 Stat1 protein protein mouse Mus musculus Mouse MSQWFELQQLDSKFLEQVHQLYDDSFPMEIRQYLAQWLEKQDWEHAAYDVSFATIRFHDLLSQLDDQYSRFSLENNFLLQHNIRKSKRNLQDNFQEDPVQMSMIIYNCLKEERKILENAQRFNQAQEGNIQNTVMLDKQKELDSKVRNVKDQVMCIEQEIKTLEELQDEYDFKCKTSQNREGEANGVAKSDQKQEQLLLHKMFLMLDNKRKEIIHKIRELLNSIELTQNTLINDELVEWKRRQQSACIGGPPNACLDQLQTWFTIVAETLQQIRQQLKKLEELEQKFTYEPDPITKNKQVLSDRTFLLFQQLIQSSFVVERQPCMPTHPQRPLVLKTGVQFTVKSRLLVKLQESNLLTKVKCHFDKDVNEKNTVKGFRKFNILGTHTKVMNMEESTNGSLAAELRHLQLKEQKNAGNRTNEGPLIVTEELHSLSFETQLCQPGLVIDLETTSLPVVVISNVSQLPSGWASILWYNMLVTEPRNLSFFLNPPCAWWSQLSEVLSWQFSSVTKRGLNADQLSMLGEKLLGPNAGPDGLIPWTRFCKENINDKNFSFWPWIDTILELIKNDLLCLWNDGCIMGFISKERERALLKDQQPGTFLLRFSESSREGAITFTWVERSQNGGEPDFHAVEPYTKKELSAVTFPDIIRNYKVMAAENIPENPLKYLYPNIDKDHAFGKYYSRPKEAPEPMELDDPKRTGYIKTELISVSEVHPSRLQTTDNLLPMSPEEFDEMSRIVGPEFDSMMSTV DIP protein P42225 original sequence version: 131 DIP protein P42225 original sequence version: 71 249D919952BE65F1 ddx58_human Antiviral innate immune response receptor RIG-I DEAD box protein 58 Retinoic acid-inducible gene 1 protein DDX58 Probable ATP-dependent RNA helicase DDX58 Retinoic acid-inducible gene I protein RIG-I-like receptor 1 protein protein human Homo sapiens Human MTTEQRRSLQAFQDYIRKTLDPTYILSYMAPWFREEEVQYIQAEKNNKGPMEAATLFLKFLLELQEEGWFRGFLDALDHAGYSGLYEAIESWDFKKIEKLEEYRLLLKRLQPEFKTRIIPTDIISDLSECLINQECEEILQICSTKGMMAGAEKLVECLLRSDKENWPKTLKLALEKERNKFSELWIVEKGIKDVETEDLEDKMETSDIQIFYQEDPECQNLSENSCPPSEVSDTNLYSPFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKGKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENNDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLDQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIATVKHNLEELEQVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRICKDLENLSQIQNREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYNDALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELESVSRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGNPKLSFLKPGILTGRGKTNQNTGMTLPAQKCILDAFKASGDHNILIATSVADEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIEKEQINMYKEKMMNDSILRLQTWDEAVFREKILHIQTHEKFIRDSQEKPKPVPDKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKECFVSRPHPKPKQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATGVQTLYSKWKDFHFEKIPFDPAEMSK BF0D501C395BAE25 stat1_human Signal transducer and activator of transcription 1-alpha/beta STAT1 Transcription factor ISGF-3 components p91/p84 protein protein human Homo sapiens Human MSQWYELQQLDSKFLEQVHQLYDDSFPMEIRQYLAQWLEKQDWEHAANDVSFATIRFHDLLSQLDDQYSRFSLENNFLLQHNIRKSKRNLQDNFQEDPIQMSMIIYSCLKEERKILENAQRFNQAQSGNIQSTVMLDKQKELDSKVRNVKDKVMCIEHEIKSLEDLQDEYDFKCKTLQNREHETNGVAKSDQKQEQLLLKKMYLMLDNKRKEVVHKIIELLNVTELTQNALINDELVEWKRRQQSACIGGPPNACLDQLQNWFTIVAESLQQVRQQLKKLEELEQKYTYEHDPITKNKQVLWDRTFSLFQQLIQSSFVVERQPCMPTHPQRPLVLKTGVQFTVKLRLLVKLQELNYNLKVKVLFDKDVNERNTVKGFRKFNILGTHTKVMNMEESTNGSLAAEFRHLQLKEQKNAGTRTNEGPLIVTEELHSLSFETQLCQPGLVIDLETTSLPVVVISNVSQLPSGWASILWYNMLVAEPRNLSFFLTPPCARWAQLSEVLSWQFSSVTKRGLNVDQLNMLGEKLLGPNASPDGLIPWTRFCKENINDKNFPFWLWIESILELIKKHLLPLWNDGCIMGFISKERERALLKDQQPGTFLLRFSESSREGAITFTWVERSQNGGEPDFHAVEPYTKKELSAVTFPDIIRNYKVMAAENIPENPLKYLYPNIDKDHAFGKYYSRPKEAPEPMELDGPKGTGYIKTELISVSEVHPSRLQTTDNLLPMSPEEFDEVSRIVGSVEFDSMMNTV 054A813522364BA6 stat1-ddx58 3 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association 5C Interferon-alpha ddx58-stat1 3 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association 5Ci Interferon-alpha 15 minutes post Interferon-alpha treatment STAT1 was phosphorylated ddx58-stat1-1 1 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association 5Ci Interferon-alpha 15 minutes post Interferon-alpha treatment STAT1 was phosphorylated stat1-ddx58-1 1 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association 5C Interferon-alpha stat1-ddx58-2 2 5 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association 5D Interferon-alpha The SHP1-STAT1 interaction was significantly enhanced by RIG-I deficiency. ptpn6-stat1 2 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association 5D Interferon-alpha The SHP1-STAT1 interaction was significantly enhanced by RIG-I deficiency.