matrixdb Institut de Biologie et de Chimie des Prot\u00c3\u00a9ines, 7, passage du Vercors 69 367 Lyon cedex 07 FRANCE matrixdb@ibcp.fr http://matrixdb.ibcp.fr Structural elucidation of full-length nidogen and the laminin-nidogen complex in solution. Structural elucidation of full-length nidogen and the laminin-nidogen complex in solution. Matrix Biol. (0945-053X) 2014 imex curation Patel T.R., Bernards C., Meier M., McEleney K., Winzor D.J., Koch M., Stetefeld J. jorg.stetefeld@ad.manitoba.ca 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro dls dynamic light scattering dls predetermined predetermined participant predetermined Patel T.R., Bernards C., Meier M., McEleney K., Winzor D.J., Koch M., Stetefeld J. jorg.stetefeld@ad.manitoba.ca Accepted 2014-JUL-25 by ORCHARD Matrix Biol. (0945-053X) 2014 Only protein-protein interactions imex curation Structural elucidation of full-length nidogen and the laminin-nidogen complex in solution. Structural elucidation of full-length nidogen and the laminin-nidogen complex in solution. Matrix Biol. (0945-053X) 2014 imex curation Patel T.R., Bernards C., Meier M., McEleney K., Winzor D.J., Koch M., Stetefeld J. jorg.stetefeld@ad.manitoba.ca 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro saxs x ray scattering saxs predetermined predetermined participant predetermined Patel T.R., Bernards C., Meier M., McEleney K., Winzor D.J., Koch M., Stetefeld J. jorg.stetefeld@ad.manitoba.ca Accepted 2014-JUL-25 by ORCHARD Matrix Biol. (0945-053X) 2014 Only protein-protein interactions imex curation Structural elucidation of full-length nidogen and the laminin-nidogen complex in solution. Structural elucidation of full-length nidogen and the laminin-nidogen complex in solution. Matrix Biol. (0945-053X) 2014 imex curation Patel T.R., Bernards C., Meier M., McEleney K., Winzor D.J., Koch M., Stetefeld J. jorg.stetefeld@ad.manitoba.ca 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Patel T.R., Bernards C., Meier M., McEleney K., Winzor D.J., Koch M., Stetefeld J. jorg.stetefeld@ad.manitoba.ca Accepted 2014-JUL-25 by ORCHARD Matrix Biol. (0945-053X) 2014 Only protein-protein interactions imex curation nid1_mouse Nidogen-1 Nid1 Ent Entactin protein protein mouse Mus musculus Mouse MLDASGCSWAMWTWALLQLLLLVGPGGCLNRQELFPFGPGQGDLELEAGDDVVSPSLELIGELSFYDRTDITSVYVTTNGIIAMSEPPATEYHPGTFPPSFGSVAPFLADLDTTDGLGNVYYREDLSPFIIQMAAEYVQRGFPEVSFQPTSVVVVTWESVAPYGGPSSSPAEEGKRNTFQAVLASSNSSSYAIFLYPEDGLQFFTTFSKKDESQVPAVVGFSKGLVGFLWKSNGAYNIFANDRESIENLAKSSNAGHQGVWVFEIGSPATAKGVVSADVNLDLDDDGADYEDEDYDLVTSHLGLEDVATPSPSHSPRRGYPDPHNVPRILSPGYEATERPRGVPTERTRSFQLPAERFPQHHPQVIDVDEVEETGVVFSYNTGSQQTCANNRHQCSVHAECRDYATGFCCRCVANYTGNGRQCVAEGSPQRVNGKVKGRIFVGSSQVPVVFENTDLHSYVVMNHGRSYTAISTIPETVGYSLLPLAPIGGIIGWMFAVEQDGFKNGFSITGGEFTRQAEVTFLGHPGKLVLKQQFSGIDEHGHLTISTELEGRVPQIPYGASVHIEPYTELYHYSSSVITSSSTREYTVMEPDQDGAAPSHTHIYQWRQTITFQECAHDDARPALPSTQQLSVDSVFVLYNKEERILRYALSNSIGPVRDGSPDALQNPCYIGTHGCDSNAACRPGPGTQFTCECSIGFRGDGQTCYDIDECSEQPSRCGNHAVCNNLPGTFRCECVEGYHFSDRGTCVAAEDQRPINYCETGLHNCDIPQRAQCIYMGGSSYTCSCLPGFSGDGRACRDVDECQHSRCHPDAFCYNTPGSFTCQCKPGYQGDGFRCMPGEVSKTRCQLEREHILGAAGGADAQRPTLQGMFVPQCDEYGHYVPTQCHHSTGYCWCVDRDGRELEGSRTPPGMRPPCLSTVAPPIHQGPVVPTAVIPLPPGTHLLFAQTGKIERLPLERNTMKKTEAKAFLHIPAKVIIGLAFDCVDKVVYWTDISEPSIGRASLHGGEPTTIIRQDLGSPEGIALDHLGRTIFWTDSQLDRIEVAKMDGTQRRVLFDTGLVNPRGIVTDPVRGNLYWTDWNRDNPKIETSHMDGTNRRILAQDNLGLPNGLTFDAFSSQLCWVDAGTHRAECLNPAQPGRRKVLEGLQYPFAVTSYGKNLYYTDWKTNSVIAMDLAISKEMDTFHPHKQTRLYGITIALSQCPQGHNYCSVNNGGCTHLCLPTPGSRTCRCPDNTLGVDCIERK 92D12D128E6EF144 lamc1_mouse Laminin subunit gamma-1 Lamc1 Lamb-2 Lamc-1 Laminin B2 chain Laminin-1 subunit gamma Laminin-2 subunit gamma Laminin-3 subunit gamma Laminin-4 subunit gamma Laminin-6 subunit gamma Laminin-7 subunit gamma Laminin-8 subunit gamma Laminin-9 subunit gamma Laminin-10 subunit gamma Laminin-11 subunit gamma S-laminin subunit gamma protein protein mouse Mus musculus Mouse MTGGGRAALALQPRGRLWPLLAVLAAVAGCVRAAMDECADEGGRPQRCMPEFVNAAFNVTVVATNTCGTPPEEYCVQTGVTGVTKSCHLCDAGQQHLQHGAAFLTDYNNQADTTWWQSQTMLAGVQYPNSINLTLHLGKAFDITYVRLKFHTSRPESFAIYKRTREDGPWIPYQYYSGSCENTYSKANRGFIRTGGDEQQALCTDEFSDISPLTGGNVAFSTLEGRPSAYNFDNSPVLQEWVTATDIRVTLNRLNTFGDEVFNEPKVLKSYYYAISDFAVGGRCKCNGHASECVKNEFDKLMCNCKHNTYGVDCEKCLPFFNDRPWRRATAESASESLPCDCNGRSQECYFDPELYRSTGHGGHCTNCRDNTDGAKCERCRENFFRLGNTEACSPCHCSPVGSLSTQCDSYGRCSCKPGVMGDKCDRCQPGFHSLTEAGCRPCSCDLRGSTDECNVETGRCVCKDNVEGFNCERCKPGFFNLESSNPKGCTPCFCFGHSSVCTNAVGYSVYDISSTFQIDEDGWRVEQRDGSEASLEWSSDRQDIAVISDSYFPRYFIAPVKFLGNQVLSYGQNLSFSFRVDRRDTRLSAEDLVLEGAGLRVSVPLIAQGNSYPSETTVKYIFRLHEATDYPWRPALSPFEFQKLLNNLTSIKIRGTYSERTAGYLDDVTLQSARPGPGVPATWVESCTCPVGYGGQFCETCLPGYRRETPSLGPYSPCVLCTCNGHSETCDPETGVCDCRDNTAGPHCEKCSDGYYGDSTLGTSSDCQPCPCPGGSSCAIVPKTKEVVCTHCPTGTAGKRCELCDDGYFGDPLGSNGPVRLCRPCQCNDNIDPNAVGNCNRLTGECLKCIYNTAGFYCDRCKEGFFGNPLAPNPADKCKACACNPYGTVQQQSSCNPVTGQCQCLPHVSGRDCGTCDPGYYNLQSGQGCERCDCHALGSTNGQCDIRTGQCECQPGITGQHCERCETNHFGFGPEGCKPCDCHHEGSLSLQCKDDGRCECREGFVGNRCDQCEENYFYNRSWPGCQECPACYRLVKDKAAEHRVKLQELESLIANLGTGDDMVTDQAFEDRLKEAEREVTDLLREAQEVKDVDQNLMDRLQRVNSSLHSQISRLQNIRNTIEETGILAERARSRVESTEQLIEIASRELEKAKMAAANVSITQPESTGEPNNMTLLAEEARRLAERHKQEADDIVRVAKTANETSAEAYNLLLRTLAGENQTALEIEELNRKYEQAKNISQDLEKQAARVHEEAKRAGDKAVEIYASVAQLTPVDSEALENEANKIKKEAADLDRLIDQKLKDYEDLREDMRGKEHEVKNLLEKGKAEQQTADQLLARADAAKALAEEAAKKGRSTLQEANDILNNLKDFDRRVNDNKTAAEEALRRIPAINRTIAEANEKTREAQLALGNAAADATEAKNKAHEAERIASAVQKNATSTKADAERTFGEVTDLDNEVNGMLRQLEEAENELKRKQDDADQDMMMAGMASQAAQEAELNARKAKNSVSSLLSQLNNLLDQLGQLDTVDLNKLNEIEGSLNKAKDEMKASDLDRKVSDLESEARKQEAAIMDYNRDIAEIIKDIHNLEDIKKTLPTGCFNTPSIEKP DIP protein P02468 original sequence version: 142 mint Binding to cells via a high affinity receptor, laminin is thought to mediate the attachment, migration and organization of cells into tissues during embryonic development by interacting with other extracellular matrix components 81B7B08E4869F242 lamc1-nid1-1 3 5 unspecified role unspecified role neutral component neutral component binding site sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain human-293 ebna Homo sapiens 293 ebna 293 ebna 4 unspecified role unspecified role neutral component neutral component human-293 ebna Homo sapiens 293 ebna 293 ebna direct interaction direct interaction Fig. 1B lamc1-nid1-2 1 5 unspecified role unspecified role neutral component neutral component binding site sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain human-293 ebna Homo sapiens 293 ebna 293 ebna 4 unspecified role unspecified role neutral component neutral component human-293 ebna Homo sapiens 293 ebna 293 ebna direct interaction direct interaction Fig. 1C lamc1-nid1-3 2 5 unspecified role unspecified role neutral component neutral component binding site sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain human-293 ebna Homo sapiens 293 ebna 293 ebna 4 unspecified role unspecified role neutral component neutral component human-293 ebna Homo sapiens 293 ebna 293 ebna direct interaction direct interaction Fig. 4A