UniProt UniProt http://www.uniprot.org Rotavirus NSP1 inhibits interferon induced non-canonical NFκB activation by interacting with TNF receptor associated factor 2. Rotavirus NSP1 inhibits interferon induced non-canonical NFκB activation by interacting with TNF receptor associated factor 2. Virology (0042-6822) 2013 imex curation Bagchi P., Bhowmick R., Nandi S., Kant Nayak M., Chawla-Sarkar M. Origin of transfected RV NSP1 according pmid 20392855, of FLAG-TRAF2 according pmid 8548810 (following Li, 2009 pmid 18952128 mentioned). sarkarmc@icmr.org.in Only protein-protein interactions imex curation human-ht_29 Homo sapiens HT-29 caucasian women with colon adenocarcinoma ht_29 HT-29 confirmed as human with IEF of AST, LDH established from the primary tumor of a 44-year-old Caucasian woman with colon adenocarcinoma in 1964; described to be heterotransplantable forming well-differentiated grade I tumors anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Bagchi P., Bhowmick R., Nandi S., Kant Nayak M., Chawla-Sarkar M. Origin of transfected RV NSP1 according pmid 20392855, of FLAG-TRAF2 according pmid 8548810 (following Li, 2009 pmid 18952128 mentioned). sarkarmc@icmr.org.in Accepted 2015-APR-24 by PPORRAS Virology (0042-6822) 2013 imex curation Rotavirus NSP1 inhibits interferon induced non-canonical NFκB activation by interacting with TNF receptor associated factor 2. Rotavirus NSP1 inhibits interferon induced non-canonical NFκB activation by interacting with TNF receptor associated factor 2. Virology (0042-6822) 2013 imex curation Bagchi P., Bhowmick R., Nandi S., Kant Nayak M., Chawla-Sarkar M. Origin of transfected RV NSP1 according pmid 20392855, of FLAG-TRAF2 according pmid 8548810 (following Li, 2009 pmid 18952128 mentioned). sarkarmc@icmr.org.in Only protein-protein interactions imex curation human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Bagchi P., Bhowmick R., Nandi S., Kant Nayak M., Chawla-Sarkar M. Origin of transfected RV NSP1 according pmid 20392855, of FLAG-TRAF2 according pmid 8548810 (following Li, 2009 pmid 18952128 mentioned). sarkarmc@icmr.org.in Accepted 2015-APR-24 by PPORRAS Virology (0042-6822) 2013 imex curation Rotavirus NSP1 inhibits interferon induced non-canonical NFκB activation by interacting with TNF receptor associated factor 2. Rotavirus NSP1 inhibits interferon induced non-canonical NFκB activation by interacting with TNF receptor associated factor 2. Virology (0042-6822) 2013 imex curation Bagchi P., Bhowmick R., Nandi S., Kant Nayak M., Chawla-Sarkar M. Origin of transfected RV NSP1 according pmid 20392855, of FLAG-TRAF2 according pmid 8548810 (following Li, 2009 pmid 18952128 mentioned). sarkarmc@icmr.org.in Only protein-protein interactions imex curation chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Bagchi P., Bhowmick R., Nandi S., Kant Nayak M., Chawla-Sarkar M. Origin of transfected RV NSP1 according pmid 20392855, of FLAG-TRAF2 according pmid 8548810 (following Li, 2009 pmid 18952128 mentioned). sarkarmc@icmr.org.in Accepted 2015-APR-24 by PPORRAS Virology (0042-6822) 2013 imex curation p89055_9reov Non-structural protein 1 NSP1 NCVP2 Non-structural RNA-binding protein 53 protein protein rotavirus sp. Rotavirus sp. MATFKDACYHYRKLNKLNGLVLKLGANDAWRPAPIAKYKGWCLDCCQHTDLTYCRGCALFHVCQWCSQYNRCFLDEEPHLLRMRTFRNQISRKDIEGLINMYNTLFPINERIVDKFISNVKQRRCRNEFLIEWYNHLLLPITLQALMVELEGDVYYIFGYFDHMEKENQTPFQFVNMINNYDKLLLDDKNFDRMTNLPVILQQEYAFRYFSKSRFISKTKKSVNRHDFANNLMEEMDNPISLMQVMRNCVNEYMDDKNWNEKCTLIVDMKSYMELMKSSYTEHYSVSQRCKLFTIYKLNIISKLIKPNYIFSNHGAHALDVHNCKWCQMNNHYKIWDDFRLKKVYNNTMCFIRALMKSNKNVGHRSSQEVVYEYMSNIFIVWKNEKWNKSMQMIFDYLEPVEISGIEYILLDHELSWEIRGIVMQIMNGEIPRILTFNDVKKIISAIIYDWFDVRYMREMPLIISTTNELRKMNKRNDLMDEYSYELSDTE 1CC82426B23E60E8 traf2_human TNF receptor-associated factor 2 TRAF2 TRAP3 Tumor necrosis factor type 2 receptor-associated protein 3 E3 ubiquitin-protein ligase TRAF2 RING-type E3 ubiquitin transferase TRAF2 protein protein human Homo sapiens Human MAAASVTPPGSLELLQPGFSKTLLGTKLEAKYLCSACRNVLRRPFQAQCGHRYCSFCLASILSSGPQNCAACVHEGIYEEGISILESSSAFPDNAARREVESLPAVCPSDGCTWKGTLKEYESCHEGRCPLMLTECPACKGLVRLGEKERHLEHECPERSLSCRHCRAPCCGADVKAHHEVCPKFPLTCDGCGKKKIPREKFQDHVKTCGKCRVPCRFHAIGCLETVEGEKQQEHEVQWLREHLAMLLSSVLEAKPLLGDQSHAGSELLQRCESLEKKTATFENIVCVLNREVERVAMTAEACSRQHRLDQDKIEALSSKVQQLERSIGLKDLAMADLEQKVLEMEASTYDGVFIWKISDFARKRQEAVAGRIPAIFSPAFYTSRYGYKMCLRIYLNGDGTGRGTHLSLFFVVMKGPNDALLRWPFNQKVTLMLLDQNNREHVIDAFRPDVTSSSFQRPVNDMNIASGCPLFCPVSKMEAKNSYVRDDAIFIKAIVDLTGL DIP protein Q12933 original sequence version: 131 DIP protein Q12933 original sequence version: 129 DIP protein Q12933 original sequence version: 167 DIP protein Q12933 original sequence version: 193 DIP protein Q12933-1 original sequence version: 154 DIP protein Q12933 original sequence version: 130 DIP protein Q12933 original sequence version: 151 C508BE185B783B20 traf2-nsp1-1 1 5 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey infection infection physical association physical association 2A nsp1-traf2-1 2 5 unspecified role unspecified role prey prey nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 2B and D nsp1-traf2-2 3 5 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role neutral component neutral component c-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization 2C