IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. J. Struct. Biol. (1047-8477) 2012 imex curation Hain A.U., Weltzer R.R., Hammond H., Jayabalasingham B., Dinglasan R.R., Graham D.R., Colquhoun D.R., Coppens I., Bosch J. jubosch@jhsph.edu 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro thermal shift thermal shift binding thermal shift predetermined predetermined participant predetermined Hain A.U., Weltzer R.R., Hammond H., Jayabalasingham B., Dinglasan R.R., Graham D.R., Colquhoun D.R., Coppens I., Bosch J. A triple cysteine mutant of Q8IJK2 (PfAtg8) (cys32ile-cys119ser-cys122ser) with significantly increased solubility was used. jubosch@jhsph.edu Accepted 2014-JUN-27 by ORCHARD J. Struct. Biol. (1047-8477) 2012 Only protein-protein interactions imex curation Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. J. Struct. Biol. (1047-8477) 2012 imex curation Hain A.U., Weltzer R.R., Hammond H., Jayabalasingham B., Dinglasan R.R., Graham D.R., Colquhoun D.R., Coppens I., Bosch J. jubosch@jhsph.edu 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down ms participant Identification by mass spectrometry ms participant Hain A.U., Weltzer R.R., Hammond H., Jayabalasingham B., Dinglasan R.R., Graham D.R., Colquhoun D.R., Coppens I., Bosch J. His6-PfAtg8 was attached to Ni-NTA resin and incubated with red blood cell stage P. falciparum lysate. In some pull down experiments, a triple cysteine mutant of Q8IJK2 (PfAtg8) (cys32ile-cys119ser-cys122ser) with significantly increased solubility was used. jubosch@jhsph.edu Accepted 2014-JUN-27 by ORCHARD J. Struct. Biol. (1047-8477) 2012 Only protein-protein interactions imex curation Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. J. Struct. Biol. (1047-8477) 2012 imex curation Hain A.U., Weltzer R.R., Hammond H., Jayabalasingham B., Dinglasan R.R., Graham D.R., Colquhoun D.R., Coppens I., Bosch J. jubosch@jhsph.edu 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Hain A.U., Weltzer R.R., Hammond H., Jayabalasingham B., Dinglasan R.R., Graham D.R., Colquhoun D.R., Coppens I., Bosch J. A triple cysteine mutant of Q8IJK2 (PfAtg8) (cys32ile-cys119ser-cys122ser) with significantly increased solubility was used. jubosch@jhsph.edu Accepted 2014-JUN-27 by ORCHARD J. Struct. Biol. (1047-8477) 2012 Only protein-protein interactions imex curation Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. J. Struct. Biol. (1047-8477) 2012 imex curation Hain A.U., Weltzer R.R., Hammond H., Jayabalasingham B., Dinglasan R.R., Graham D.R., Colquhoun D.R., Coppens I., Bosch J. jubosch@jhsph.edu 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro dls dynamic light scattering dls predetermined predetermined participant predetermined Hain A.U., Weltzer R.R., Hammond H., Jayabalasingham B., Dinglasan R.R., Graham D.R., Colquhoun D.R., Coppens I., Bosch J. jubosch@jhsph.edu Accepted 2014-JUN-27 by ORCHARD J. Struct. Biol. (1047-8477) 2012 Only protein-protein interactions imex curation Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. J. Struct. Biol. (1047-8477) 2012 imex curation Hain A.U., Weltzer R.R., Hammond H., Jayabalasingham B., Dinglasan R.R., Graham D.R., Colquhoun D.R., Coppens I., Bosch J. jubosch@jhsph.edu 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Hain A.U., Weltzer R.R., Hammond H., Jayabalasingham B., Dinglasan R.R., Graham D.R., Colquhoun D.R., Coppens I., Bosch J. jubosch@jhsph.edu Accepted 2014-JUN-27 by ORCHARD J. Struct. Biol. (1047-8477) 2012 Only protein-protein interactions imex curation Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. J. Struct. Biol. (1047-8477) 2012 imex curation Hain A.U., Weltzer R.R., Hammond H., Jayabalasingham B., Dinglasan R.R., Graham D.R., Colquhoun D.R., Coppens I., Bosch J. jubosch@jhsph.edu 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 anti tag coip anti tag coimmunoprecipitation anti tag coip weight by comassie molecular weight estimation by coomasie staining weight by comassie Hain A.U., Weltzer R.R., Hammond H., Jayabalasingham B., Dinglasan R.R., Graham D.R., Colquhoun D.R., Coppens I., Bosch J. A triple cysteine mutant of Q8IJK2 (PfAtg8) (cys32ile-cys119ser-cys122ser) with significantly increased solubility was used. jubosch@jhsph.edu Accepted 2014-JUN-27 by ORCHARD J. Struct. Biol. (1047-8477) 2012 Only protein-protein interactions imex curation Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. J. Struct. Biol. (1047-8477) 2012 imex curation Hain A.U., Weltzer R.R., Hammond H., Jayabalasingham B., Dinglasan R.R., Graham D.R., Colquhoun D.R., Coppens I., Bosch J. jubosch@jhsph.edu 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Hain A.U., Weltzer R.R., Hammond H., Jayabalasingham B., Dinglasan R.R., Graham D.R., Colquhoun D.R., Coppens I., Bosch J. In some SPR experiments, a triple cysteine mutant of Q8IJK2 (PfAtg8) (cys32ile-cys119ser-cys122ser) with significantly increased solubility was used. jubosch@jhsph.edu Accepted 2014-JUN-27 by ORCHARD J. Struct. Biol. (1047-8477) 2012 Only protein-protein interactions imex curation Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. Structural characterization and inhibition of the Plasmodium Atg8-Atg3 interaction. J. Struct. Biol. (1047-8477) 2012 imex curation Hain A.U., Weltzer R.R., Hammond H., Jayabalasingham B., Dinglasan R.R., Graham D.R., Colquhoun D.R., Coppens I., Bosch J. jubosch@jhsph.edu 21-Jul-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Hain A.U., Weltzer R.R., Hammond H., Jayabalasingham B., Dinglasan R.R., Graham D.R., Colquhoun D.R., Coppens I., Bosch J. jubosch@jhsph.edu Accepted 2014-JUN-27 by ORCHARD J. Struct. Biol. (1047-8477) 2012 Only protein-protein interactions imex curation nbd-chloride 4-Chloro-7-nitrobenzofurazan small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) q8ijk2_plaf7 Autophagy-related protein PF3D7_1019900 protein protein plaf7 Plasmodium falciparum (isolate 3D7) MPSLKDEVSFENRVAETHKIRSKYPNRIPVVCERANRSNLPIIEKKKFLVPMNMLVGEFKFILHQHINQSAYGSNMKLFRERTIYLFVNNIVPKTGLLMQDLYEMYKDEDGYLYMEYSCESCLG 89C8F0B42C6B96B6 4-nitrocatechol 4-nitrocatechol 1,2-Dihydroxy-4-nitrobenzene 4-Nitrocatechol 4-nitrocatechol OK 4-Nitropyrocatechol 4-nitrobenzene-1,2-diol small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) XJNPNXSISMKQEX-UHFFFAOYSA-N XJNPNXSISMKQEX-UHFFFAOYSA-N pyrogallol pyrogallol 1,2,3-Benzenetriol 1,2,3-Trihydroxybenzene benzene-1,2,3-triol Pyrogallic acid Pyrogallol benzene-1,2,3-triol small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) WQGWDDDVZFFDIG-UHFFFAOYSA-N WQGWDDDVZFFDIG-UHFFFAOYSA-N 1,3-indandione 1,3-indandione small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) esculetin esculetin 6,7-dihydroxy-2H-1-benzopyran-2-one 6,7-dihydroxycoumarin aesculetin cichorigenin cichoriin aglucon cichoriin aglycon esculetin Esculetin esculin aglucon esculin aglycon 6,7-dihydroxy-2H-chromen-2-one small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) ILEDWLMCKZNDJK-UHFFFAOYSA-N ILEDWLMCKZNDJK-UHFFFAOYSA-N c0h519_plaf7 PF3D7_0905700.2 protein protein plaf7 Plasmodium falciparum (isolate 3D7) MSDQINVKHKIGDTCRKLYSYFKTVNNTSTFIQNGTLTPSEFVDSGDFLVYKFKTWEWQEADKDRVVPYLPENKQFLITKNVPCKQRIKDLNNIVHDLKIVDNDWLLPSYEEDNNPTDIYEYLPNSEYTINDKNIYNYEEEEEDDNCDEAIDINNFYMENNLIKEHDPASINSTSCYSKNMLHDNLMKIRTYDVSITYDKYYQTPRIWLFGYNENGDPLKSEEIFEDILSDYSYKTVTYDPHPCTGVMTASIHPCKHAEAILNVVNNWISEEKEPRHDLYLLFLLKFISGVIPTIEYDFTTDIEIPRDSNAGL 6486C7424F2119A1 benzene-1,2,4-triol benzene-1,2,4-triol 1,2,4-benzenetriol 1,2,4-Benzenetriol 1,2,4-Trihydroxybenzene Benzene-1,2,4-triol Hydroxyhydroquinone Hydroxyquinol benzene-1,2,4-triol small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) GGNQRNBDZQJCCN-UHFFFAOYSA-N GGNQRNBDZQJCCN-UHFFFAOYSA-N c0h519-q8ijk2 3 PfAtg3 15 unspecified role unspecified role neutral component neutral component lig_lir_apic_2 motif sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) PfAtg8 10 unspecified role unspecified role neutral component neutral component ubiquitin-related domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q8IJK2:p.[Cys32Ile;Cys119Ser;Cys122Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain C I certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S Wed May 02 15:36:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys32ile,cys119ser,cys122ser' Tue Jun 30 20:14:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Cys32Ile;Cys119Ser;Cys122Ser]' gamg-tag n-terminus tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 19 21 direct interaction direct interaction Table 1 2.2A working 20.5%, free 27.9% c0h519-q8ijk2-1 6 PfAtg8 10 unspecified role unspecified role prey prey Q8IJK2:p.[Cys32Ile;Cys119Ser;Cys122Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain C I certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S Wed May 02 17:14:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys32ile,cys119ser,cys122ser' Tue Jun 30 19:45:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Cys32Ile;Cys119Ser;Cys122Ser]' n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range PfAtg3 15 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range C0H519:p.Trp105Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain W A Wed May 02 16:29:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'trp105ala' Tue Jun 30 16:20:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Trp105Ala' physical association physical association Fig. 1b c0h519-q8ijk2-2 7 PfAtg3 15 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 PfAtg8 10 unspecified role unspecified role prey prey n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q8IJK2:p.[Cys32Ile;Cys119Ser;Cys122Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain C I certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S Wed May 02 16:45:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys32ile,cys119ser,cys122ser' Tue Jun 30 20:26:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Cys32Ile;Cys119Ser;Cys122Ser]' 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 direct interaction direct interaction Fig. 1d, Fig 5c, Fig. 5d, Fig. 5e Temp = 25C PfAtg3 (C0H519) binds to wild-type PfAtg8 (Q8IJK2) with Kd = 290 x 10e-9 molar, k-on = 54.3 x 10e+5 per molar per second, and k-off = 0.13 per second. PfAtg3 (C0H519) binds to the triple cys mutant of PfAtg8 (Q8IJK2) (cys32ile-cys119ser-cys122ser) with Kd = 326 x 10e-9 molar, k-on = 5.1 x 10e+5 per molar per second, and k-off = 0.17 per second. c0h519-c0h519 7 PfAtg3 15 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 PfAtg3 15 competitor competitor competitor prey prey lig_lir_apic_2 motif sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) PfAtg8 10 unspecified role unspecified role prey prey n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q8IJK2:p.[Cys32Ile;Cys119Ser;Cys122Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain C I certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S Wed May 02 15:10:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys32ile,cys119ser,cys122ser' Tue Jun 30 19:49:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Cys32Ile;Cys119Ser;Cys122Ser]' 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 physical association physical association Fig. 1d q8ijk2-c0h519 1 10 unspecified role unspecified role bait bait Q8IJK2:p.[Cys32Ile;Cys119Ser;Cys122Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C I Fri Jul 22 11:01:02 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'cys119ser,cys122ser,cys32ile' Wed May 02 16:40:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys32ile,cys119ser,cys122ser' Tue Jun 30 17:08:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Cys32Ile;Cys119Ser;Cys122Ser]' n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 PfAtg3 15 unspecified role unspecified role prey prey lig_lir_apic_2 motif sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Fig. 1c q8ijk2-c0h519-1 2 PfAtg3 15 unspecified role unspecified role prey prey plaf7 Plasmodium falciparum (isolate 3D7) PfAtg8 10 unspecified role unspecified role bait bait n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q8IJK2:p.[Cys32Ile;Cys119Ser;Cys122Ser] mutation mutation certain certain sequence position certain certain certain sequence position certain C I certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S Wed May 02 15:07:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys32ile,cys119ser,cys122ser' Tue Jun 30 19:45:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Cys32Ile;Cys119Ser;Cys122Ser]' 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 physical association physical association Fig. 4b, Fig. S7 c0h519-q8ijk2-3 4 PfAtg8 10 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 PfAtg3 15 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 direct interaction direct interaction Fig. 5b c0h519-q8ijk2-4 5 PfAtg8 10 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 PfAtg3 15 unspecified role unspecified role neutral component neutral component n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 direct interaction direct interaction Fig. 5a c0h519-q8ijk2-5 8 PfAtg3 15 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 PfAtg8 10 unspecified role unspecified role prey prey Q8IJK2:p.Leu49Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L A Wed May 02 15:49:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu49ala' Tue Jun 30 19:46:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu49Ala' Q8IJK2:p.Arg27Glu mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R E Wed May 02 17:54:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg27glu' Tue Jun 30 16:12:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg27Glu' Q8IJK2:p.Glu44_Lys46delinsAlaSerAla mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain EKK ASA Thu Jul 07 12:07:32 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was glu44ala-lys45ser-lys46ala Fri Jul 22 10:53:44 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'glu44ala,lys45ser,lys46ala' Wed May 02 17:23:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu_lys_lys44-46ala_ser_ala' Tue Jun 30 19:49:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu44_Lys46delinsAlaSerAla' Q8IJK2:p.Lys47Glu mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K E Wed May 02 14:42:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys47glu' Tue Jun 30 17:51:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys47Glu' Q8IJK2:p.[Lys19Glu;Lys47Glu] mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K E certain certain sequence position certain certain certain sequence position certain K E Wed May 02 15:38:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys19glu,lys47glu' Tue Jun 30 20:00:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys19Glu;Lys47Glu]' n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 direct interaction direct interaction Fig. 6c c0h519-benzene_1_2_4_triol 7 PfAtg3 15 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 F408 16 inhibitor inhibitor prey prey PfAtg8 10 unspecified role unspecified role prey prey n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q8IJK2:p.[Cys32Ile;Cys119Ser;Cys122Ser] mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain C I certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S Wed May 02 17:30:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys32ile,cys119ser,cys122ser' Tue Jun 30 15:46:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Cys32Ile;Cys119Ser;Cys122Ser]' 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 physical association physical association Fig. 7b c0h519-pyrogallol 7 PfAtg8 10 unspecified role unspecified role prey prey n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q8IJK2:p.[Cys32Ile;Cys119Ser;Cys122Ser] mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain C I certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S Wed May 02 19:27:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys32ile,cys119ser,cys122ser' Tue Jun 30 15:55:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Cys32Ile;Cys119Ser;Cys122Ser]' 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 PfAtg3 15 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 F410 12 inhibitor inhibitor prey prey physical association physical association Fig. 7b q8ijk2-benzene_1_2_4_triol 7 F408 16 unspecified role unspecified role prey prey PfAtg8 10 unspecified role unspecified role bait bait Q8IJK2:p.[Lys19Glu;Lys47Glu] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K E certain certain sequence position certain certain certain sequence position certain K E Wed May 02 18:12:36 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys19glu,lys47glu' Tue Jun 30 18:47:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys19Glu;Lys47Glu]' n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 direct interaction direct interaction Suppl. Fig. S9, Table 2 q8ijk2-pyrogallol 7 PfAtg8 10 unspecified role unspecified role bait bait n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q8IJK2:p.[Lys19Glu;Lys47Glu] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K E certain certain sequence position certain certain certain sequence position certain K E Wed May 02 14:29:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys19glu,lys47glu' Tue Jun 30 17:50:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys19Glu;Lys47Glu]' 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 F410 12 unspecified role unspecified role prey prey direct interaction direct interaction Suppl. Fig. S9, Table 2 q8ijk2-4_nitrocatechol 7 PfAtg8 10 unspecified role unspecified role bait bait n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q8IJK2:p.[Lys19Glu;Lys47Glu] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K E certain certain sequence position certain certain certain sequence position certain K E Wed May 02 18:39:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys19glu,lys47glu' Tue Jun 30 18:40:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys19Glu;Lys47Glu]' 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 F537 11 unspecified role unspecified role prey prey direct interaction direct interaction Suppl. Fig. S9, Table 2 q8ijk2-1_3_indandione 7 F792 13 unspecified role unspecified role prey prey PfAtg8 10 unspecified role unspecified role bait bait n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q8IJK2:p.[Lys19Glu;Lys47Glu] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K E certain certain sequence position certain certain certain sequence position certain K E Wed May 02 17:19:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys19glu,lys47glu' Wed May 02 18:51:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys19glu,lys47glu' Tue Jun 30 18:31:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys19Glu;Lys47Glu]' 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 direct interaction direct interaction Suppl. Fig. S9, Table 2 q8ijk2-esculetin 7 PfAtg8 10 unspecified role unspecified role bait bait n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q8IJK2:p.[Lys19Glu;Lys47Glu] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K E certain certain sequence position certain certain certain sequence position certain K E Wed May 02 15:34:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys19glu,lys47glu' Tue Jun 30 16:42:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys19Glu;Lys47Glu]' 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 F268 14 unspecified role unspecified role prey prey direct interaction direct interaction Suppl. Fig. S9, Table 2 q8ijk2-nbd_chloride 7 PfAtg8 10 unspecified role unspecified role bait bait n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q8IJK2:p.[Lys19Glu;Lys47Glu] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain K E certain certain sequence position certain certain certain sequence position certain K E Wed May 02 14:30:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys19glu,lys47glu' Tue Jun 30 17:00:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys19Glu;Lys47Glu]' 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 F470 9 unspecified role unspecified role prey prey direct interaction direct interaction Suppl. Fig. S9, Table 2 q8ijk2-esculetin-1 1 F268 14 unspecified role unspecified role prey prey PfAtg8 10 unspecified role unspecified role bait bait n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q8IJK2:p.[Cys32Ile;Cys119Ser;Cys122Ser] mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain C I certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S Wed May 02 14:37:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys32ile,cys119ser,cys122ser' Tue Jun 30 16:20:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Cys32Ile;Cys119Ser;Cys122Ser]' 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 direct interaction direct interaction Fig. 7a q8ijk2-4_nitrocatechol-1 1 PfAtg8 10 unspecified role unspecified role bait bait n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Q8IJK2:p.[Cys32Ile;Cys119Ser;Cys122Ser] mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain C I certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S Wed May 02 18:40:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys32ile,cys119ser,cys122ser' Tue Jun 30 19:41:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Cys32Ile;Cys119Ser;Cys122Ser]' 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 F537 11 unspecified role unspecified role prey prey direct interaction direct interaction Fig. 7a q8ijk2-benzene_1_2_4_triol-1 1 F408 16 unspecified role unspecified role prey prey 10 unspecified role unspecified role bait bait Q8IJK2:p.[Cys32Ile;Cys119Ser;Cys122Ser] mutation mutation mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain C I certain certain sequence position certain certain certain sequence position certain C S certain certain sequence position certain certain certain sequence position certain C S Wed May 02 14:30:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys32ile,cys119ser,cys122ser' Tue Jun 30 20:11:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Cys32Ile;Cys119Ser;Cys122Ser]' n-terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 direct interaction direct interaction Fig. 7a c0h519-pyrogallol-1 7 F410 12 unspecified role unspecified role prey prey PfAtg3 15 unspecified role unspecified role bait bait n-terminus mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 562-rosetta ii de3 Escherichia coli rosetta ii de3 rosetta ii de3 direct interaction direct interaction Suppl. Fig. S11