matrixdb Institut de Biologie et de Chimie des Prot\u00c3\u00a9ines, 7, passage du Vercors 69 367 Lyon cedex 07 FRANCE matrixdb@ibcp.fr http://matrixdb.ibcp.fr Collagen XXIII, novel ligand for integrin alpha2beta1 in the epidermis. Collagen XXIII, novel ligand for integrin alpha2beta1 in the epidermis. J. Biol. Chem. (0021-9258) 2011 imex curation Veit G., Zwolanek D., Eckes B., Niland S., Käpylä J., Zweers M.C., Ishada-Yamamoto A., Krieg T., Heino J., Eble J.A., Koch M. manuel.koch@uni-koeln.de mouse-embryonic_skin Mouse embryonic skin embryonic_skin Embryonic skin fluorescence imaging fluorescence microscopy fluorescence imaging immunohistochemistry immunohistochemistry Veit G., Zwolanek D., Eckes B., Niland S., Käpylä J., Zweers M.C., Ishada-Yamamoto A., Krieg T., Heino J., Eble J.A., Koch M. manuel.koch@uni-koeln.de Accepted 2016-JUN-23 AT 08:50 BST AT 08:50 BST by ORCHARD J. Biol. Chem. (0021-9258) 2011 imex curation Collagen XXIII, novel ligand for integrin alpha2beta1 in the epidermis. Collagen XXIII, novel ligand for integrin alpha2beta1 in the epidermis. J. Biol. Chem. (0021-9258) 2011 imex curation Veit G., Zwolanek D., Eckes B., Niland S., Käpylä J., Zweers M.C., Ishada-Yamamoto A., Krieg T., Heino J., Eble J.A., Koch M. manuel.koch@uni-koeln.de in vitro In vitro solid phase assay solid phase assay antibody detection identification by antibody antibody detection Veit G., Zwolanek D., Eckes B., Niland S., Käpylä J., Zweers M.C., Ishada-Yamamoto A., Krieg T., Heino J., Eble J.A., Koch M. manuel.koch@uni-koeln.de Accepted 2016-JUN-23 AT 08:50 BST AT 08:50 BST by ORCHARD J. Biol. Chem. (0021-9258) 2011 imex curation Collagen XXIII, novel ligand for integrin alpha2beta1 in the epidermis. Collagen XXIII, novel ligand for integrin alpha2beta1 in the epidermis. J. Biol. Chem. (0021-9258) 2011 imex curation Veit G., Zwolanek D., Eckes B., Niland S., Käpylä J., Zweers M.C., Ishada-Yamamoto A., Krieg T., Heino J., Eble J.A., Koch M. manuel.koch@uni-koeln.de mouse_tongue Mouse-tongue tongue Tongue fluorescence imaging fluorescence microscopy fluorescence imaging immunohistochemistry immunohistochemistry Veit G., Zwolanek D., Eckes B., Niland S., Käpylä J., Zweers M.C., Ishada-Yamamoto A., Krieg T., Heino J., Eble J.A., Koch M. manuel.koch@uni-koeln.de Accepted 2016-JUN-23 AT 08:50 BST AT 08:50 BST by ORCHARD J. Biol. Chem. (0021-9258) 2011 imex curation Collagen XXIII, novel ligand for integrin alpha2beta1 in the epidermis. Collagen XXIII, novel ligand for integrin alpha2beta1 in the epidermis. J. Biol. Chem. (0021-9258) 2011 imex curation Veit G., Zwolanek D., Eckes B., Niland S., Käpylä J., Zweers M.C., Ishada-Yamamoto A., Krieg T., Heino J., Eble J.A., Koch M. manuel.koch@uni-koeln.de in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Veit G., Zwolanek D., Eckes B., Niland S., Käpylä J., Zweers M.C., Ishada-Yamamoto A., Krieg T., Heino J., Eble J.A., Koch M. manuel.koch@uni-koeln.de Accepted 2016-JUN-23 AT 08:50 BST AT 08:50 BST by ORCHARD J. Biol. Chem. (0021-9258) 2011 imex curation Mock publication and experiment for abstract interactions that are not interaction evidences. Mock publication and experiment for abstract interactions that are not interaction evidences. unspecified method Collagen XXIII, novel ligand for integrin alpha2beta1 in the epidermis. Collagen XXIII, novel ligand for integrin alpha2beta1 in the epidermis. J. Biol. Chem. (0021-9258) 2011 imex curation Veit G., Zwolanek D., Eckes B., Niland S., Käpylä J., Zweers M.C., Ishada-Yamamoto A., Krieg T., Heino J., Eble J.A., Koch M. manuel.koch@uni-koeln.de mouse-skin Mus musculus skin cells skin fluorescence imaging fluorescence microscopy fluorescence imaging immunohistochemistry immunohistochemistry Veit G., Zwolanek D., Eckes B., Niland S., Käpylä J., Zweers M.C., Ishada-Yamamoto A., Krieg T., Heino J., Eble J.A., Koch M. manuel.koch@uni-koeln.de Accepted 2016-JUN-23 AT 08:50 BST AT 08:50 BST by ORCHARD J. Biol. Chem. (0021-9258) 2011 imex curation ita2_mouse Integrin alpha-2 Itga2 Platelet membrane glycoprotein Ia Collagen receptor CD49 antigen-like family member B VLA-2 subunit alpha protein protein mouse Mus musculus Mouse MGPGQAGGALLLRLLMLVQGILNCLAYNVGLPGAKIFSGPSSEQFGYSVQQLTNPQGNWLLVGSPWSGFPENRMGDVYKCPVDLPTATCEKLNLQNSASISNVTEIKTNMSLGLTLTRNPGTGGFLTCGPLWAHQCGNQYYATGICSDVSPDFQFLTSFSPAVQACPSLVDVVVVCDESNSIYPWEAVKNFLVKFVTGLDIGPKKTQVALIQYANEPRIIFNLNDFETKEDMVQATSETRQHGGDLTNTFRAIEFARDYAYSQTSGGRPGATKVMVVVTDGESHDGSKLKTVIQQCNDDEILRFGIAVLGYLNRNALDTKNLIKEIKAIASTPTERYFFNVADEAALLEKAGTLGEQIFSIEGTVQGGDNFQMEMAQVGFSADYAPQNDILMLGAVGAFDWSGTLVQETSHKPVIFPKQAFDQVLQDRNHSSFLGYSVAAISTEDGVHFVAGAPRANYTGQIVLYSVNKQGNVTVIQSHRGDQIGSYFGSVLCSVDVDKDTITDVLLVGAPTYMNDLKKEEGKVYLFTITKGILNQHQFLEGPEGTGNARFGSAIAALSDINMDGFNDVIVGSPVENENSGAVYIYNGHQGTIRTKYSQKILGSNGAFRRHLQFFGRSLDGYGDLNGDSITDVSIGALGQVIQLWSQSIADVAIEALFTPDKITLLNKDAKITLKLCFRAEFRPAGQNNQVAILFNMTLDADGHSSRVTSRGVFRENSERFLQKNMVVNEVQKCSEHHISIQKPSDVVNPLDLRVDISLENPGTSPALEAYSETVKVFSIPFYKECGSDGICISDLILDVQQLPAIQTQSFIVSNQNKRLTFSVILKNRGESAYNTVVLAEFSENLFFASFSMPVDGTEVTCEVGSSQKSVTCDVGYPALKSEQQVTFTINFDFNLQNLQNQAAINFQAFSESQETNKADNSVSLTIPLLYDAELHLTRSTNINFYEISSDENAPSVIKSVEDIGPKFIFSLKVTAGSAPVSMALVTIHIPQYTKEKNPLLYLTGIQTDQAGDISCTAEINPLKLPHTAPSVSFKNENFRHTKELDCRTTSCSNITCWLKDLHMKAEYFINVTTRVWNRTFAASTFQTVQLTAAAEIDTHNPQLFVIEENAVTIPLMIMKPTEKAEVPTGVIIGSIIAGILLLLAMTAGLWKLGFFKRQYKKMGQNPDEMDETTELNS mint Integrin alpha-2/beta-1 is a collagen receptor, being responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. It is also a receptor for laminins, collagen C-propeptides and E-cadherin. Mice homozygous for a null mutation in the alpha-2 die very early in embryogenesis 62FAEA820242A9B6 cona1_mouse Collagen alpha-1(XXIII) chain Col23a1 protein protein mouse Mus musculus Mouse MGAGERAAGGGGAQDPGAGCGSRALSALCLLLSVGSAAACLLLGAQAAALHGRVAALEQERELLRHAGPSGALAAWAETHLERLLREKLDGVAKLRTVREAPSECICPPGPPGRRGKPGRRGDPGPPGQSGRDGYPGPLGLDGKPGLPGPKGEKGTPGDFGPRGAQGQDGVAGPPGPPGPPGARGPPGDTGKDGPRGAQGPAGPRGEPGQDGEMGPKGPPGPKGEPGIPGKKGDDGMANQPGLPGPPGPKGEPGDVGPRGENGVDGIPGLKGEPGHPGVDGATGPRGAPGLKGEQGDTVVIDYDGRILDALKGPPGPQGAPGPPGIPGAKGELGLPGAPGIDGEKGPKGPKGDPGEPGPAGPKGETGEMGLSGLPGADGPKGEKGESASDHLQESLAQIIVEPGPPGPPGPPGPMGLQGIQGPKGLDGAKGEKGTSGERGPHGLPGPVGPPGLIGLPGTKGEKGRPGEPGLDGFPGPRGEKGDRSERGEKGERGVPGRKGVKGQKGEPGPPGLDQPCPVGPDGLPVPGCWHK 035E8538D5642486 itb1_human Integrin beta-1 Fibronectin receptor subunit beta VLA-4 subunit beta ITGB1 FNRB MDF2 MSK12 Glycoprotein IIa protein protein human Homo sapiens Human MNLQPIFWIGLISSVCCVFAQTDENRCLKANAKSCGECIQAGPNCGWCTNSTFLQEGMPTSARCDDLEALKKKGCPPDDIENPRGSKDIKKNKNVTNRSKGTAEKLKPEDITQIQPQQLVLRLRSGEPQTFTLKFKRAEDYPIDLYYLMDLSYSMKDDLENVKSLGTDLMNEMRRITSDFRIGFGSFVEKTVMPYISTTPAKLRNPCTSEQNCTSPFSYKNVLSLTNKGEVFNELVGKQRISGNLDSPEGGFDAIMQVAVCGSLIGWRNVTRLLVFSTDAGFHFAGDGKLGGIVLPNDGQCHLENNMYTMSHYYDYPSIAHLVQKLSENNIQTIFAVTEEFQPVYKELKNLIPKSAVGTLSANSSNVIQLIIDAYNSLSSEVILENGKLSEGVTISYKSYCKNGVNGTGENGRKCSNISIGDEVQFEISITSNKCPKKDSDSFKIRPLGFTEEVEVILQYICECECQSEGIPESPKCHEGNGTFECGACRCNEGRVGRHCECSTDEVNSEDMDAYCRKENSSEICSNNGECVCGQCVCRKRDNTNEIYSGKFCECDNFNCDRSNGLICGGNGVCKCRVCECNPNYTGSACDCSLDTSTCEASNGQICNGRGICECGVCKCTDPKFQGQTCEMCQTCLGVCAEHKECVQCRAFNKGEKKDTCTQECSYFNITKVESRDKLPQPVQPDPVSHCKEKDVDDCWFYFTYSVNGNNEVMVHVVENPECPTGPDIIPIVAGVVAGIVLIGLALLLIWKLLMIIHDRREFAKFEKEKMNAKWDTGENPIYKSAVTTVVNPKYEGK DIP protein P05556 original sequence version: 139 DIP protein P05556-1 original sequence version: 143 DIP protein P05556 original sequence version: 154 DIP protein P05556 original sequence version: 167 DE35979C1625578C ita2_human Integrin alpha-2 Platelet membrane glycoprotein Ia Collagen receptor VLA-2 subunit alpha CD49 antigen-like family member B ITGA2 CD49B protein protein human Homo sapiens Human MGPERTGAAPLPLLLVLALSQGILNCCLAYNVGLPEAKIFSGPSSEQFGYAVQQFINPKGNWLLVGSPWSGFPENRMGDVYKCPVDLSTATCEKLNLQTSTSIPNVTEMKTNMSLGLILTRNMGTGGFLTCGPLWAQQCGNQYYTTGVCSDISPDFQLSASFSPATQPCPSLIDVVVVCDESNSIYPWDAVKNFLEKFVQGLDIGPTKTQVGLIQYANNPRVVFNLNTYKTKEEMIVATSQTSQYGGDLTNTFGAIQYARKYAYSAASGGRRSATKVMVVVTDGESHDGSMLKAVIDQCNHDNILRFGIAVLGYLNRNALDTKNLIKEIKAIASIPTERYFFNVSDEAALLEKAGTLGEQIFSIEGTVQGGDNFQMEMSQVGFSADYSSQNDILMLGAVGAFGWSGTIVQKTSHGHLIFPKQAFDQILQDRNHSSYLGYSVAAISTGESTHFVAGAPRANYTGQIVLYSVNENGNITVIQAHRGDQIGSYFGSVLCSVDVDKDTITDVLLVGAPMYMSDLKKEEGRVYLFTIKKGILGQHQFLEGPEGIENTRFGSAIAALSDINMDGFNDVIVGSPLENQNSGAVYIYNGHQGTIRTKYSQKILGSDGAFRSHLQYFGRSLDGYGDLNGDSITDVSIGAFGQVVQLWSQSIADVAIEASFTPEKITLVNKNAQIILKLCFSAKFRPTKQNNQVAIVYNITLDADGFSSRVTSRGLFKENNERCLQKNMVVNQAQSCPEHIIYIQEPSDVVNSLDLRVDISLENPGTSPALEAYSETAKVFSIPFHKDCGEDGLCISDLVLDVRQIPAAQEQPFIVSNQNKRLTFSVTLKNKRESAYNTGIVVDFSENLFFASFSLPVDGTEVTCQVAASQKSVACDVGYPALKREQQVTFTINFDFNLQNLQNQASLSFQALSESQEENKADNLVNLKIPLLYDAEIHLTRSTNINFYEISSDGNVPSIVHSFEDVGPKFIFSLKVTTGSVPVSMATVIIHIPQYTKEKNPLMYLTGVQTDKAGDISCNADINPLKIGQTSSSVSFKSENFRHTKELNCRTASCSNVTCWLKDVHMKGEYFVNVTTRIWNGTFASSTFQTVQLTAAAEINTYNPEIYVIEDNTVTIPLMIMKPDEKAEVPTGVIIGSIIAGILLLLALVAILWKLGFFKRKYEKMTKNPDEIDETTELSS DIP protein P17301 original sequence version: 108 DIP protein P17301 original sequence version: 186 7E1B7ED968A94070 itga2-collagen-2 2 10 unspecified role unspecified role bait bait tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range P17301:p.Glu347Trp mutation mutation predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain E W Wed May 02 16:56:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu347trp' Tue Jun 30 20:16:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu347Trp' binding site sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 17 unspecified role unspecified role prey prey q8k4g2 binding site sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged predetermined featur predetermined feature predetermined featur c-term range C-terminal range c-term range c-term range C-terminal range c-term range tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 ebna Homo sapiens 293 ebna 293 ebna direct interaction direct interaction Figure 6A collagen-itga2-1 2 17 unspecified role unspecified role bait bait q8k4g2 binding site sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged predetermined featur predetermined feature predetermined featur c-term range C-terminal range c-term range c-term range C-terminal range c-term range tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 ebna Homo sapiens 293 ebna 293 ebna 10 unspecified role unspecified role prey prey tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag exp feature detect experimental feature detection exp feature detect n-term range n-terminal range n-term range n-term range n-terminal range n-term range binding site sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain P17301:p.Glu347Trp mutation mutation predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain E W Wed May 02 17:00:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu347trp' Tue Jun 30 16:45:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu347Trp' ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 6B itga2-collagen-1 4 17 unspecified role unspecified role prey prey binding site sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged predetermined featur predetermined feature predetermined featur c-term range C-terminal range c-term range c-term range C-terminal range c-term range tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 ebna Homo sapiens 293 ebna 293 ebna 10 unspecified role unspecified role bait bait P17301:p.Glu347Trp mutation mutation predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain E W Wed May 02 19:19:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu347trp' Tue Jun 30 18:04:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu347Trp' tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range binding site sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Figure 6C Langmuir 1:1 binding model intg-collagen 4 41 unspecified role unspecified role bait bait drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. 17 unspecified role unspecified role prey prey binding site sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur c-term range C-terminal range c-term range c-term range C-terminal range c-term range tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged predetermined featur predetermined feature predetermined featur c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 ebna Homo sapiens 293 ebna 293 ebna direct interaction direct interaction Figure 7C Interaction of collagen XXIII with the heterodimeric integrin alpha2beta1 ectodomain (alpha2: mature ectodomain fused to dimerizing motif of the transcription factor Fos, beta1: 21-728 fused to dimerizing Jun alpha helix) collagen-intg 2 17 unspecified role unspecified role bait bait tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged predetermined featur predetermined feature predetermined featur c-term range C-terminal range c-term range c-term range C-terminal range c-term range tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur c-term range C-terminal range c-term range c-term range C-terminal range c-term range q8k4g2 binding site sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain human-293 ebna Homo sapiens 293 ebna 293 ebna 41 unspecified role unspecified role prey prey drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. Interaction of collagen XXIII with the heterodimeric integrin alpha2beta1 ectodomain (alpha2: mature ectodomain fused to dimerizing motif of the transcription factor Fos, beta1: 21-728 fused to dimerizing Jun alpha helix) direct interaction direct interaction Figure 7A, Figure 7D The absence of binding after addition of EDTA revealed the dependence on bivalent cations commonly seen in integrin-collagen interactions and furthermore indicated the specificity of the observed interaction A prerequisite for the interaction of collagen to collagen- binding integrins is the presence of an intact collagen triple helix folding.Disruption of the native folding strongly reduced binding to soluble integrin alpha2beta1 (Figure 7D). intg-collagen-1 2 41 unspecified role unspecified role bait bait drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. Interaction of collagen XXIII with the heterodimeric integrin alpha2beta1 ectodomain (alpha2: mature ectodomain fused to dimerizing motif of the transcription factor Fos, beta1: 21-728 fused to dimerizing Jun alpha helix) 17 unspecified role unspecified role prey prey tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag predetermined featur predetermined feature predetermined featur c-term range C-terminal range c-term range c-term range C-terminal range c-term range q8k4g2 binding site sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged predetermined featur predetermined feature predetermined featur c-term range C-terminal range c-term range c-term range C-terminal range c-term range human-293 ebna Homo sapiens 293 ebna 293 ebna direct interaction direct interaction Figure 7B 1:1 interaction collagen-itga2-2 1 7 unspecified role unspecified role neutral component neutral component 17 unspecified role unspecified role neutral component neutral component colocalization colocalization Figure 1A collagen-itga2-3 6 7 unspecified role unspecified role neutral component neutral component 17 unspecified role unspecified role neutral component neutral component colocalization colocalization Figure 1B collagen-itga2-4 3 7 unspecified role unspecified role neutral component neutral component 17 unspecified role unspecified role neutral component neutral component colocalization colocalization Figure 1C collagen_xxiii_mouse Type XXIII collagen Collagen type XXIII trimer Type XXIII collagen Col23a1 trimer 8 unspecified role unspecified role direct interaction direct interaction mouse Mus musculus Mouse stable complex stable complex biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion biological system reconstruction evidence based on inference from background scientific knowledge used in manual assertion Type II orientated transmembrane collagen. Homotrimer Triple-helices consisting of about 1000 amino acids per chain forming a coiled coil structure. Each polypeptide forms a left-handed helix in which every third residue, glycine, comes into the centre of the superhelix. created with intact-jami-dbscripts intg_a2_b1_human Integrin alpha-2/beta-1 alpha-2/beta-1 integrin complex ITGA2:ITGB1 Integrin alpha2-beta1 complex alpha2-beta1 integrin complex 9 unspecified role unspecified role 10 unspecified role unspecified role direct interaction direct interaction human Homo sapiens Human stable complex stable complex physical interaction evidence used in manual assertion physical interaction evidence used in manual assertion IPI inferred from physical interaction Cell adhesion, bi-directional signaling receptor which functions as a link between the extra-cellular matrix and the cytoskeleton. Receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the sequence G-F-P-G-E-R in a wide array of ligands. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. The ligand-binding site forms in a region at the intersection of the integrin alpha-chain beta-propeller and the betaI domain, with the alpha chain being central in determining ligand specificity. Heterodimer created with intact-jami-dbscripts