IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A stress-responsive system for mitochondrial protein degradation. A stress-responsive system for mitochondrial protein degradation. Mol. Cell (1097-2765) 2010 imex curation Heo J.M., Livnat-Levanon N., Taylor E.B., Jones K.T., Dephoure N., Ring J., Xie J., Brodsky J.L., Madeo F., Gygi S.P., Ashrafi K., Glickman M.H., Rutter J. rutter@biochem.utah.edu Only protein-protein interactions mouse-c2c12 Mouse C3H muscle myoblast c2c12 Mouse C3H muscle myoblast tap tandem affinity purification TAP TAP tap western blot western blot Immuno blot Immuno blot Heo J.M., Livnat-Levanon N., Taylor E.B., Jones K.T., Dephoure N., Ring J., Xie J., Brodsky J.L., Madeo F., Gygi S.P., Ashrafi K., Glickman M.H., Rutter J. rutter@biochem.utah. rutter@biochem.utah.edu Accepted 2015-MAR-24 by PPORRAS Mol. Cell (1097-2765) 2010 Only protein-protein interactions imex curation A stress-responsive system for mitochondrial protein degradation. A stress-responsive system for mitochondrial protein degradation. Mol. Cell (1097-2765) 2010 imex curation Heo J.M., Livnat-Levanon N., Taylor E.B., Jones K.T., Dephoure N., Ring J., Xie J., Brodsky J.L., Madeo F., Gygi S.P., Ashrafi K., Glickman M.H., Rutter J. rutter@biochem.utah.edu Only protein-protein interactions mouse-c2c12 Mouse C3H muscle myoblast c2c12 Mouse C3H muscle myoblast tap tandem affinity purification TAP TAP tap sequence tag sequence tag identification sequence tag Heo J.M., Livnat-Levanon N., Taylor E.B., Jones K.T., Dephoure N., Ring J., Xie J., Brodsky J.L., Madeo F., Gygi S.P., Ashrafi K., Glickman M.H., Rutter J. rutter@biochem.utah. rutter@biochem.utah.edu Accepted 2015-MAR-24 by PPORRAS Mol. Cell (1097-2765) 2010 Only protein-protein interactions imex curation A stress-responsive system for mitochondrial protein degradation. A stress-responsive system for mitochondrial protein degradation. Mol. Cell (1097-2765) 2010 imex curation Heo J.M., Livnat-Levanon N., Taylor E.B., Jones K.T., Dephoure N., Ring J., Xie J., Brodsky J.L., Madeo F., Gygi S.P., Ashrafi K., Glickman M.H., Rutter J. rutter@biochem.utah.edu Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast tap tandem affinity purification TAP TAP tap sequence tag sequence tag identification sequence tag Heo J.M., Livnat-Levanon N., Taylor E.B., Jones K.T., Dephoure N., Ring J., Xie J., Brodsky J.L., Madeo F., Gygi S.P., Ashrafi K., Glickman M.H., Rutter J. rutter@biochem.utah. rutter@biochem.utah.edu Accepted 2015-MAR-24 by PPORRAS Mol. Cell (1097-2765) 2010 Only protein-protein interactions imex curation A stress-responsive system for mitochondrial protein degradation. A stress-responsive system for mitochondrial protein degradation. Mol. Cell (1097-2765) 2010 imex curation Heo J.M., Livnat-Levanon N., Taylor E.B., Jones K.T., Dephoure N., Ring J., Xie J., Brodsky J.L., Madeo F., Gygi S.P., Ashrafi K., Glickman M.H., Rutter J. rutter@biochem.utah.edu Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Heo J.M., Livnat-Levanon N., Taylor E.B., Jones K.T., Dephoure N., Ring J., Xie J., Brodsky J.L., Madeo F., Gygi S.P., Ashrafi K., Glickman M.H., Rutter J. rutter@biochem.utah. rutter@biochem.utah.edu Accepted 2015-MAR-24 by PPORRAS Mol. Cell (1097-2765) 2010 Only protein-protein interactions imex curation npl4_yeast Nuclear protein localization protein 4 NPL4 YBR170C YBR1231 HRD4 HMG-CoA reductase degradation protein 4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MLIRFRSKNGTHRVSCQENDLFGTVIEKLVGNLDPNADVDTFTVCEKPGQGIHAVSELADRTVMDLGLKHGDMLILNYSDKPANEKDGVNVEIGSVGIDSKGIRQHRYGPLRIKELAVDEELEKEDGLIPRQKSKLCKHGDRGMCEYCSPLPPWDKEYHEKNKIKHISFHSYLKKLNENANKKENGSSYISPLSEPDFRINKRCHNGHEPWPRGICSKCQPSAITLQQQEFRMVDHVEFQKSEIINEFIQAWRYTGMQRFGYMYGSYSKYDNTPLGIKAVVEAIYEPPQHDEQDGLTMDVEQVKNEMLQIDRQAQEMGLSRIGLIFTDLSDAGAGDGSVFCKRHKDSFFLSSLEVIMAARHQTRHPNVSKYSEQGFFSSKFVTCVISGNLEGEIDISSYQVSTEAEALVTADMISGSTFPSMAYINDTTDERYVPEIFYMKSNEYGITVKENAKPAFPVDYLLVTLTHGFPNTDTETNSKFVSSTGFPWSNRQAMGQSQDYQELKKYLFNVASSGDFNLLHEKISNFHLLLYINSLQILSPDEWKLLIESAVKNEWEESLLKLVSSAGWQTLVMILQESG 6EF35D21338EE07E 6EF35D21338EE07E ankz1_mouse Ankyrin repeat and zinc finger domain-containing protein 1 Ankzf1 D1Ertd161e protein protein mouse Mus musculus Mouse MAKPAGDAAVGSRSGELFLPSVSSSATSPAPSAAPAPASVSLLSLNGEAPLIRGLSLVSQAPGEALAWAPRTSCPGENTSSGGKVSPYSLEISEKLFCSACDQIFQNHQEQREHYKLDWHRFNLKQRLKNKPLLSASDFEQQSSTGDLSSISGSDDTDSSSEEDLLPLDEGRAESEKPNRPPGFYPHRVLFKNAQGQFLYAYRCVLGPHQIPPEKAELLLQNLQNGGPRYYVVLMAAAGHFAGAIFQGREVVAHKTFHRYTVRAKRGTAQGLQDAHGRASRSAGANLRRYNEAMLYKDVRNLLAGPIWSKALGEAETVLFRAPRSGRSLFFGGQGAPLQRDDPRLWDIPLTTRRPTFGELQRVLHKLTTLQVYDEDPREMVRFHSPETHWKPVREERKKDTEKEKTKVPSDANKPLGQDEEPLKQGSESQEEDGSEVELELVELTLGTLDLREFEVLPKRRRRRKKKERSQEQQCGAHGPLPQQPQDEPFSQPTQEVETPLDTLVYEAKAPGQPELWDTLLAACRAGEVEVLKLQLATGLVDPGVKSLLNAPLGSGGFTLLHAAAAAGRGLVVRLLLEAGADPTVQDSRARPPYTVAADKSTRNEFRRFMEKNLDAYDYNKARVPGPLTQEMEARQATRKKEQKAARRQREQQQRKQREQEEQEQEEQRRFAALSDREKRALAAERRLAAQLGAPSPPVPDSAVASAGRCWSCGVSLQGLIPFHYLDFSFCSTRCLRDHRSQAGRPSS 38E877B35B4A7594 vms1_yeast Protein VMS1 YDR049W VMS1 VCP/CDC48-associated mitochondrial stress-responsive protein 1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MNSQKASKMTGSLKKNDLYIFDLSEQLLNSLKLMSFDSTLREVEVEKTSDNDRNKESGDLQIARKKVTSNVMRCSVCQMSFDSRNEQKAHYQTDYHLMNVKRNLRGLDILSVEEFDALISKEHGIKSEDENSGGEQTSSDHEESEEASDRDPDLQTNNYMETIIENDLQKLGFQKDESDAISHINTQSPYIYFKSKYLQKNEVLAIYKSLFNKRSLSNPNEALTFWNSQENPMAISALFMVGGGHFAGAIVSHQRLNVKGNAHKKDETLIEQAVNFLEHKTFHRYTTRRKQGGSQSAMDNAKGKANSAGSALRRYNESALKTDIQGVLKDWEPYLSKCDNIFIRARNVSDKKIFTDNTVLNKGDERIKSFPFTTNRPTVLELKKAWCELSYLKILPKPEPLAVKETVQKLEVSNKKDEFKEKQEPLLEEIQTEEIISLLKKGRAPLLISFLKKNKLDGNFRLKPESKYSLTPTMLHYASQQGMKQMALILLSNIKCDPTIKNRLGRTAWDLNRNDDVRHAFQIARYNLGESFTNWDETHIGQPLSREQVDEINEKKKAIENEKAEKLIKLELEAAKEKQRFAKDAERGPGKKLTNIPSIQQQNLNSLTDEQRRRLMREQRARAAEERMKKKY 98A2007E5591A220 98A2007E5591A220 ufd1_yeast Ubiquitin fusion degradation protein 1 UFD1 PIP3 Polymerase-interacting protein 3 YGR048W protein protein yeast Saccharomyces cerevisiae Baker's yeast MFSGFSSFGGGNGFVNMPQTFEEFFRCYPIAMMNDRIRKDDANFGGKIFLPPSALSKLSMLNIRYPMLFKLTANETGRVTHGGVLEFIAEEGRVYLPQWMMETLGIQPGSLLQISSTDVPLGQFVKLEPQSVDFLDISDPKAVLENVLRNFSTLTVDDVIEISYNGKTFKIKILEVKPESSSKSICVIETDLVTDFAPPVGYVEPDYKALKAQQDKEKKNSFGKGQVLDPSVLGQGSMSTRIDYAGIANSSRNKLSKFVGQGQNISGKAPKAEPKQDIKDMKITFDGEPAKLDLPEGQLFFGFPMVLPKEDEESAAGSKSSEQNFQGQGISLRKSNKRKTKSDHDSSKSKAPKSPEVIEID 198E0CD48B863B98 198E0CD48B863B98 tera_mouse Transitional endoplasmic reticulum ATPase Vcp 15S Mg(2+)-ATPase p97 subunit Valosin-containing protein protein protein mouse Mus musculus Mouse MASGADSKGDDLSTAILKQKNRPNRLIVDEAINEDNSVVSLSQPKMDELQLFRGDTVLLKGKKRREAVCIVLSDDTCSDEKIRMNRVVRNNLRVRLGDVISIQPCPDVKYGKRIHVLPIDDTVEGITGNLFEVYLKPYFLEAYRPIRKGDIFLVRGGMRAVEFKVVETDPSPYCIVAPDTVIHCEGEPIKREDEEESLNEVGYDDIGGCRKQLAQIKEMVELPLRHPALFKAIGVKPPRGILLYGPPGTGKTLIARAVANETGAFFFLINGPEIMSKLAGESESNLRKAFEEAEKNAPAIIFIDELDAIAPKREKTHGEVERRIVSQLLTLMDGLKQRAHVIVMAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEILQIHTKNMKLADDVDLEQVANETHGHVGADLAALCSEAALQAIRKKMDLIDLEDETIDAEVMNSLAVTMDDFRWALSQSNPSALRETVVEVPQVTWEDIGGLEDVKRELQELVQYPVEHPDKFLKFGMTPSKGVLFYGPPGCGKTLLAKAIANECQANFISIKGPELLTMWFGESEANVREIFDKARQAAPCVLFFDELDSIAKARGGNIGDGGGAADRVINQILTEMDGMSTKKNVFIIGATNRPDIIDPAILRPGRLDQLIYIPLPDEKSRVAILKANLRKSPVAKDVDLEFLAKMTNGFSGADLTEICQRACKLAIRESIESEIRRERERQTNPSAMEVEEDDPVPEIRRDHFEEAMRFARRSVSDNDIRKYEMFAQTLQQSRGFGSFRFPSGNQGGAGPSQGSGGGTGGSVYTEDNDDDLYG DIP protein Q01853 original sequence version: 84 DIP protein Q01853 original sequence version: 112 DIP protein Q01853 original sequence version: 152 DIP protein Q01853 original sequence version: 136 DIP protein Q01853 original sequence version: 156 DIP protein Q01853 original sequence version: 149 501B721D3A77BA8A cdc48_yeast Cell division control protein 48 CDC48 Cell division cycle protein 48 Transitional endoplasmic reticulum ATPase homolog YDL126C protein protein yeast Saccharomyces cerevisiae Baker's yeast MGEEHKPLLDASGVDPREEDKTATAILRRKKKDNMLLVDDAINDDNSVIAINSNTMDKLELFRGDTVLVKGKKRKDTVLIVLIDDELEDGACRINRVVRNNLRIRLGDLVTIHPCPDIKYATRISVLPIADTIEGITGNLFDVFLKPYFVEAYRPVRKGDHFVVRGGMRQVEFKVVDVEPEEYAVVAQDTIIHWEGEPINREDEENNMNEVGYDDIGGCRKQMAQIREMVELPLRHPQLFKAIGIKPPRGVLMYGPPGTGKTLMARAVANETGAFFFLINGPEVMSKMAGESESNLRKAFEEAEKNAPAIIFIDEIDSIAPKRDKTNGEVERRVVSQLLTLMDGMKARSNVVVIAATNRPNSIDPALRRFGRFDREVDIGIPDATGRLEVLRIHTKNMKLADDVDLEALAAETHGYVGADIASLCSEAAMQQIREKMDLIDLDEDEIDAEVLDSLGVTMDNFRFALGNSNPSALRETVVESVNVTWDDVGGLDEIKEELKETVEYPVLHPDQYTKFGLSPSKGVLFYGPPGTGKTLLAKAVATEVSANFISVKGPELLSMWYGESESNIRDIFDKARAAAPTVVFLDELDSIAKARGGSLGDAGGASDRVVNQLLTEMDGMNAKKNVFVIGATNRPDQIDPAILRPGRLDQLIYVPLPDENARLSILNAQLRKTPLEPGLELTAIAKATQGFSGADLLYIVQRAAKYAIKDSIEAHRQHEAEKEVKVEGEDVEMTDEGAKAEQEPEVDPVPYITKEHFAEAMKTAKRSVSDAELRRYEAYSQQMKASRGQFSNFNFNDAPLGTTATDNANSNNSAPSGAGAAFGSNAEEDDDLYS 02ADDB9A227614D8 02ADDB9A227614D8 vms1-cdc48-2 3 10 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait c-terminal region tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 4A vms1-cdc48-3 4 10 unspecified role unspecified role prey prey c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range P25694:p.Ser565Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S E Wed May 02 16:01:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser565glu' Tue Jun 30 17:42:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser565Glu' 7 unspecified role unspecified role bait bait c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range vims region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain c-terminal sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain physical association physical association Fig. 4B, 4E, 5B, Supp Fig 4A vms1-npl4 4 7 unspecified role unspecified role bait bait c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain vims region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 5 unspecified role unspecified role prey prey c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 4C, 4F, 5C, Supp. Fig. 4A ufd1-npl4-2 4 8 unspecified role unspecified role bait bait c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 5 unspecified role unspecified role prey prey c-terminal myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Fig. 4D ankzf1-vcp-2 2 6 unspecified role unspecified role bait bait c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role prey prey physical association physical association Fig. 5E ankzf1-vcp-1 1 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q80UU1:p.Glu685_Arg687delinsAlaAlaAla mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain ERR AAA Wed May 02 17:41:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu_arg_arg685-687ala_ala_ala' Tue Jun 30 17:04:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu685_Arg687delinsAlaAlaAla' physical association physical association Fig. 5F