UniProt UniProt http://www.uniprot.org Functional conservation of Mei4 for meiotic DNA double-strand break formation from yeasts to mice. Functional conservation of Mei4 for meiotic DNA double-strand break formation from yeasts to mice. Genes Dev. (0890-9369) 2010 mimix curation Kumar R., Bourbon H.M., de Massy B. bernard.de-massy@igh.cnrs.fr 21-Jul-2014: Contacted by IntAct-Help. human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Kumar R., Bourbon H.M., de Massy B. bernard.de-massy@igh.cnrs.fr Accepted 2014-JUL-15 by MGT Genes Dev. (0890-9369) 2010 mimix curation Functional conservation of Mei4 for meiotic DNA double-strand break formation from yeasts to mice. Functional conservation of Mei4 for meiotic DNA double-strand break formation from yeasts to mice. Genes Dev. (0890-9369) 2010 mimix curation Kumar R., Bourbon H.M., de Massy B. bernard.de-massy@igh.cnrs.fr 21-Jul-2014: Contacted by IntAct-Help. yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Kumar R., Bourbon H.M., de Massy B. bernard.de-massy@igh.cnrs.fr Accepted 2014-JUL-15 by MGT Genes Dev. (0890-9369) 2010 mimix curation Functional conservation of Mei4 for meiotic DNA double-strand break formation from yeasts to mice. Functional conservation of Mei4 for meiotic DNA double-strand break formation from yeasts to mice. Genes Dev. (0890-9369) 2010 mimix curation Kumar R., Bourbon H.M., de Massy B. bernard.de-massy@igh.cnrs.fr 21-Jul-2014: Contacted by IntAct-Help. in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Kumar R., Bourbon H.M., de Massy B. bernard.de-massy@igh.cnrs.fr Accepted 2014-JUL-15 by MGT Genes Dev. (0890-9369) 2010 mimix curation mei4_mouse Meiosis-specific protein MEI4 Mei4 protein protein mouse Mus musculus Mouse MDIQPWYLKTSKLALALAIIHSKPADRSSREYTEYLASLVTQKESTWKSKLEALEAEVLQLRQKLLLSRISSGLFKNGPDVLPTLSDQEPTSSENTLTLMDDSGCVLSNEQRNEPAELSQHFVESTDPPLLPLPLEKRPRTTLENPLSSHMQFFQHLLELKKWTESSSLKVYLTHFEKDSSTVSDSVSQLLDALITFYRNPKLPFSSFWTEAVGTLARLASDFNLSNHIFKRCSKKLEEFEKTLLQAILENNSINRFQVQRYVSQSLVTLGSCSLLRKSIISLLLSEVNSFVDDLGAIDQDQGIYDVTRYENIFSLFWILEQVLQQAPQGDRTAHMDHSIPEMQTFLQKHDEVIFRLSDAFPLFAFYLWRLGVLLNSAEMETVKNESLP 04B41F13A4A3FCD1 re114_mouse Meiotic recombination protein REC114 Rec114 protein protein mouse Mus musculus Mouse MSEAGNVASGLGLPGEVSQWSLKRYGRFMLLDNVGSPGPSSEAAAAGSPTWKVFESSEESGSLVLTIVVSGHFFISQGQTLLEGFSLIGSKNWLKIVRRMDCLLFGTTIKNKSRMFRVQFSGESKEEALERCCGCVQTLAQYVTVQEPDSTTQELQQSQGPREAGESQGKDPLQQGPSLTLEQHVCMAAGAGVLQERTSVTHRAQSILAPEKLTLAYEGSSWGTEELGPFLRLCLMDQNFPAFVEEVEKELKKITGLRN 26FB5D90CA9B010D mei4-r114l-2 1 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association 3A bottom r114l-mei4-2 1 5 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association S5A r114l-mei4-1 2 5 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association 3B and S6A mei4-r114l-1 2 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait physical association physical association 3B and S6B mei4-r114l-4 3 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait direct interaction direct interaction S5B