HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html A single-amino-acid mutation in hepatitis C virus NS5A disrupting FKBP8 interaction impairs viral replication. A single-amino-acid mutation in hepatitis C virus NS5A disrupting FKBP8 interaction impairs viral replication. J. Virol. (0022-538X) 2008 imex curation Okamoto T., Omori H., Kaname Y., Abe T., Nishimura Y., Suzuki T., Miyamura T., Yoshimori T., Moriishi K., Matsuura Y. Virus - Publications including interactions involving viral proteins Source of non-viral cDNAs inferred from previous work of the same author PMID:17024179 matsuura@biken.osaka-u.ac.jp 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Okamoto T., Omori H., Kaname Y., Abe T., Nishimura Y., Suzuki T., Miyamura T., Yoshimori T., Moriishi K., Matsuura Y. Virus - Publications including interactions involving viral proteins Source of non-viral cDNAs inferred from previous work of the same author PMID:17024179 matsuura@biken.osaka-u.ac.jp Accepted 2014-JAN-29 by ORCHARD J. Virol. (0022-538X) 2008 imex curation A single-amino-acid mutation in hepatitis C virus NS5A disrupting FKBP8 interaction impairs viral replication. A single-amino-acid mutation in hepatitis C virus NS5A disrupting FKBP8 interaction impairs viral replication. J. Virol. (0022-538X) 2008 imex curation Okamoto T., Omori H., Kaname Y., Abe T., Nishimura Y., Suzuki T., Miyamura T., Yoshimori T., Moriishi K., Matsuura Y. Virus - Publications including interactions involving viral proteins Source of non-viral cDNAs inferred from previous work of the same author PMID:17024179 matsuura@biken.osaka-u.ac.jp 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Okamoto T., Omori H., Kaname Y., Abe T., Nishimura Y., Suzuki T., Miyamura T., Yoshimori T., Moriishi K., Matsuura Y. Virus - Publications including interactions involving viral proteins Source of non-viral cDNAs inferred from previous work of the same author PMID:17024179 matsuura@biken.osaka-u.ac.jp Accepted 2014-JAN-29 by ORCHARD J. Virol. (0022-538X) 2008 imex curation A single-amino-acid mutation in hepatitis C virus NS5A disrupting FKBP8 interaction impairs viral replication. A single-amino-acid mutation in hepatitis C virus NS5A disrupting FKBP8 interaction impairs viral replication. J. Virol. (0022-538X) 2008 imex curation Okamoto T., Omori H., Kaname Y., Abe T., Nishimura Y., Suzuki T., Miyamura T., Yoshimori T., Moriishi K., Matsuura Y. Virus - Publications including interactions involving viral proteins Source of non-viral cDNAs inferred from previous work of the same author PMID:17024179 matsuura@biken.osaka-u.ac.jp 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma confocal microscopy confocal microscopy immunostaining immunostaining Okamoto T., Omori H., Kaname Y., Abe T., Nishimura Y., Suzuki T., Miyamura T., Yoshimori T., Moriishi K., Matsuura Y. Virus - Publications including interactions involving viral proteins Source of non-viral cDNAs inferred from previous work of the same author PMID:17024179 matsuura@biken.osaka-u.ac.jp Accepted 2014-JAN-29 by ORCHARD J. Virol. (0022-538X) 2008 imex curation A single-amino-acid mutation in hepatitis C virus NS5A disrupting FKBP8 interaction impairs viral replication. A single-amino-acid mutation in hepatitis C virus NS5A disrupting FKBP8 interaction impairs viral replication. J. Virol. (0022-538X) 2008 imex curation Okamoto T., Omori H., Kaname Y., Abe T., Nishimura Y., Suzuki T., Miyamura T., Yoshimori T., Moriishi K., Matsuura Y. Virus - Publications including interactions involving viral proteins Source of non-viral cDNAs inferred from previous work of the same author PMID:17024179 matsuura@biken.osaka-u.ac.jp 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Okamoto T., Omori H., Kaname Y., Abe T., Nishimura Y., Suzuki T., Miyamura T., Yoshimori T., Moriishi K., Matsuura Y. Virus - Publications including interactions involving viral proteins Source of non-viral cDNAs inferred from previous work of the same author PMID:17024179 matsuura@biken.osaka-u.ac.jp Accepted 2014-JAN-29 by ORCHARD J. Virol. (0022-538X) 2008 imex curation A single-amino-acid mutation in hepatitis C virus NS5A disrupting FKBP8 interaction impairs viral replication. A single-amino-acid mutation in hepatitis C virus NS5A disrupting FKBP8 interaction impairs viral replication. J. Virol. (0022-538X) 2008 imex curation Okamoto T., Omori H., Kaname Y., Abe T., Nishimura Y., Suzuki T., Miyamura T., Yoshimori T., Moriishi K., Matsuura Y. Virus - Publications including interactions involving viral proteins Source of non-viral cDNAs inferred from previous work of the same author PMID:17024179 matsuura@biken.osaka-u.ac.jp 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Okamoto T., Omori H., Kaname Y., Abe T., Nishimura Y., Suzuki T., Miyamura T., Yoshimori T., Moriishi K., Matsuura Y. Virus - Publications including interactions involving viral proteins Source of non-viral cDNAs inferred from previous work of the same author PMID:17024179 matsuura@biken.osaka-u.ac.jp Accepted 2014-JAN-29 by ORCHARD J. Virol. (0022-538X) 2008 imex curation A single-amino-acid mutation in hepatitis C virus NS5A disrupting FKBP8 interaction impairs viral replication. A single-amino-acid mutation in hepatitis C virus NS5A disrupting FKBP8 interaction impairs viral replication. J. Virol. (0022-538X) 2008 imex curation Okamoto T., Omori H., Kaname Y., Abe T., Nishimura Y., Suzuki T., Miyamura T., Yoshimori T., Moriishi K., Matsuura Y. Virus - Publications including interactions involving viral proteins Source of non-viral cDNAs inferred from previous work of the same author PMID:17024179 matsuura@biken.osaka-u.ac.jp 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-huh7 Homo sapiens hepatoma cell line established from a hepatocellular carcinoma huh7 Human hepatoma cell line established from a hepatocellular carcinoma electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy immunostaining immunostaining Okamoto T., Omori H., Kaname Y., Abe T., Nishimura Y., Suzuki T., Miyamura T., Yoshimori T., Moriishi K., Matsuura Y. Virus - Publications including interactions involving viral proteins Source of non-viral cDNAs inferred from previous work of the same author PMID:17024179 matsuura@biken.osaka-u.ac.jp Accepted 2014-JAN-29 by ORCHARD J. Virol. (0022-538X) 2008 imex curation fkbp8_human Peptidyl-prolyl cis-trans isomerase FKBP8 FK506-binding protein 8 38 kDa FK506-binding protein FKBPR38 FKBP8 FKBP38 Rotamase protein protein human Homo sapiens Human MASCAEPSEPSAPLPAGVPPLEDFEVLDGVEDAEGEEEEEEEEEEEDDLSELPPLEDMGQPPAEEAEQPGALAREFLAAMEPEPAPAPAPEEWLDILGNGLLRKKTLVPGPPGSSRPVKGQVVTVHLQTSLENGTRVQEEPELVFTLGDCDVIQALDLSVPLMDVGETAMVTADSKYCYGPQGRSPYIPPHAALCLEVTLKTAVDGPDLEMLTGQERVALANRKRECGNAHYQRADFVLAANSYDLAIKAITSSAKVDMTFEEEAQLLQLKVKCLNNLAASQLKLDHYRAALRSCSLVLEHQPDNIKALFRKGKVLAQQGEYSEAIPILRAALKLEPSNKTIHAELSKLVKKHAAQRSTETALYRKMLGNPSRLPAKCPGKGAWSIPWKWLFGATAVALGGVALSVVIAARN DIP protein Q14318 original sequence version: 145 887C25ADE71EBA8D q9wmx2-pro_0000037551 Non-structural protein 5A protein protein hcvco Hepatitis C virus genotype 1b (isolate Con1) (HCV) SGSWLRDVWDWICTVLTDFKTWLQSKLLPRLPGVPFFSCQRGYKGVWRGDGIMQTTCPCGAQITGHVKNGSMRIVGPRTCSNTWHGTFPINAYTTGPCTPSPAPNYSRALWRVAAEEYVEVTRVGDFHYVTGMTTDNVKCPCQVPAPEFFTEVDGVRLHRYAPACKPLLREEVTFLVGLNQYLVGSQLPCEPEPDVAVLTSMLTDPSHITAETAKRRLARGSPPSLASSSASQLSAPSLKATCTTRHDSPDADLIEANLLWRQEMGGNITRVESENKVVILDSFEPLQAEEDEREVSVPAEILRRSRKFPRAMPIWARPDYNPPLLESWKDPDYVPPVVHGCPLPPAKAPPIPPPRRKRTVVLSESTVSSALAELATKTFGSSESSAVDSGTATASPDQPSDDGDAGSDVESYSSMPPLEGEPGDPDLSDGSWSTVSEEASEDVVCC 1973 2419 AC8324B2DE0F7FAE ns5a-fkbp8-12 5 8 unspecified role unspecified role bait bait c-terminal his tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey n-terminal his tag his L-histidine residue (2S)-2-amino-3-(1H-imidazol-4-yl)propanoic acid (2S)-2-amino-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 2-azanyl-3-(1H-imidazol-4-yl)propanoic acid 2-azanyl-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 4-(2-amino-2-carboxyethyl)imidazole L-histidine alpha-amino-beta-(4-imidazole)propionic acid glyoxaline-5-alanine His n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1B and 1C fkbp8-ns5a-14 5 8 unspecified role unspecified role prey prey c-terminal his tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait n-terminal his tag his L-histidine residue (2S)-2-amino-3-(1H-imidazol-4-yl)propanoic acid (2S)-2-amino-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 2-azanyl-3-(1H-imidazol-4-yl)propanoic acid 2-azanyl-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 4-(2-amino-2-carboxyethyl)imidazole L-histidine alpha-amino-beta-(4-imidazole)propionic acid glyoxaline-5-alanine His n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1B and 1D ns5a-fkbp8-13 2 8 unspecified role unspecified role bait bait c-terminal his tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role prey prey n-terminal his tag his L-histidine residue (2S)-2-amino-3-(1H-imidazol-4-yl)propanoic acid (2S)-2-amino-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 2-azanyl-3-(1H-imidazol-4-yl)propanoic acid 2-azanyl-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 4-(2-amino-2-carboxyethyl)imidazole L-histidine alpha-amino-beta-(4-imidazole)propionic acid glyoxaline-5-alanine His n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1E ns5a-fkbp8-9 1 7 unspecified role unspecified role prey prey flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait Q9WMX2-PRO_0000037551:p.Val121Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain V A Wed May 02 16:19:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val121ala' Tue Jun 30 16:53:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val121Ala' c-terminal his tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 2B and 2C ns5a-fkbp8-10 3 7 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection Q9WMX2-PRO_0000037551:p.Val121Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain V A Wed May 02 15:16:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val121ala' Tue Jun 30 18:16:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val121Ala' colocalization colocalization Fig. 3 fkbp8-ns5a-10 3 7 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 6C Cells contain an HCV subgenomic replicon fkbp8-ns5a-11 6 7 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 7A fkbp8-ns5a-12 4 8 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 7A