UniProt UniProt http://www.uniprot.org Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor. Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor. J. Cell Biol. (0021-9525) 2004 imex curation Arighi C.N., Hartnell L.M., Aguilar R.C., Haft C.R., Bonifacino J.S. Origin of CI-MPR/IGF2R according sequence coordinates fig 1. Origin of myc-SNX2 and specifity of VPS26 antibodies according Haft et al 2000, pmid:11102511 juan@helix.nih.gov 05-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions human-m1 Homo sapiens fibroblast cell line m1 M1 human fibrobast murine myeloid cell line confocal microscopy confocal microscopy immunostaining immunostaining Arighi C.N., Hartnell L.M., Aguilar R.C., Haft C.R., Bonifacino J.S. Origin of CI-MPR/IGF2R according sequence coordinates fig 1. Origin of myc-SNX2 and specifity of VPS26 antibodies according Haft et al 2000, pmid:11102511 juan@helix.nih.gov Accepted 2014-NOV-12 by MGT J. Cell Biol. (0021-9525) 2004 imex curation Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor. Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor. J. Cell Biol. (0021-9525) 2004 imex curation Arighi C.N., Hartnell L.M., Aguilar R.C., Haft C.R., Bonifacino J.S. Origin of CI-MPR/IGF2R according sequence coordinates fig 1. Origin of myc-SNX2 and specifity of VPS26 antibodies according Haft et al 2000, pmid:11102511 juan@helix.nih.gov 05-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Arighi C.N., Hartnell L.M., Aguilar R.C., Haft C.R., Bonifacino J.S. Origin of CI-MPR/IGF2R according sequence coordinates fig 1. Origin of myc-SNX2 and specifity of VPS26 antibodies according Haft et al 2000, pmid:11102511 juan@helix.nih.gov Accepted 2014-NOV-12 by MGT J. Cell Biol. (0021-9525) 2004 imex curation Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor. Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor. J. Cell Biol. (0021-9525) 2004 imex curation Arighi C.N., Hartnell L.M., Aguilar R.C., Haft C.R., Bonifacino J.S. Origin of CI-MPR/IGF2R according sequence coordinates fig 1. Origin of myc-SNX2 and specifity of VPS26 antibodies according Haft et al 2000, pmid:11102511 juan@helix.nih.gov 05-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy antibody detection identification by antibody antibody detection Arighi C.N., Hartnell L.M., Aguilar R.C., Haft C.R., Bonifacino J.S. A protein A–gold conjugate was used to visualize the presence of antibody. anti-hVpS26 (10-nm gold particles) anti-CI-MPR (15-nm gold particles) Origin of CI-MPR/IGF2R according sequence coordinates fig 1. Origin of myc-SNX2 and specifity of VPS26 antibodies according Haft et al 2000, pmid:11102511 juan@helix.nih.gov Accepted 2014-NOV-12 by MGT J. Cell Biol. (0021-9525) 2004 imex curation Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor. Role of the mammalian retromer in sorting of the cation-independent mannose 6-phosphate receptor. J. Cell Biol. (0021-9525) 2004 imex curation Arighi C.N., Hartnell L.M., Aguilar R.C., Haft C.R., Bonifacino J.S. Origin of CI-MPR/IGF2R according sequence coordinates fig 1. Origin of myc-SNX2 and specifity of VPS26 antibodies according Haft et al 2000, pmid:11102511 juan@helix.nih.gov 05-Jan-2015: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy immunostaining immunostaining Arighi C.N., Hartnell L.M., Aguilar R.C., Haft C.R., Bonifacino J.S. Origin of CI-MPR/IGF2R according sequence coordinates fig 1. Origin of myc-SNX2 and specifity of VPS26 antibodies according Haft et al 2000, pmid:11102511 juan@helix.nih.gov Accepted 2014-NOV-12 by MGT J. Cell Biol. (0021-9525) 2004 imex curation snx2_human Sorting nexin-2 Transformation-related gene 9 protein SNX2 TRG9 protein protein human Homo sapiens Human MAAEREPPPLGDGKPTDFEDLEDGEDLFTSTVSTLESSPSSPEPASLPAEDISANSNGPKPTEVVLDDDREDLFAEATEEVSLDSPEREPILSSEPSPAVTPVTPTTLIAPRIESKSMSAPVIFDRSREEIEEEANGDIFDIEIGVSDPEKVGDGMNAYMAYRVTTKTSLSMFSKSEFSVKRRFSDFLGLHSKLASKYLHVGYIVPPAPEKSIVGMTKVKVGKEDSSSTEFVEKRRAALERYLQRTVKHPTLLQDPDLRQFLESSELPRAVNTQALSGAGILRMVNKAADAVNKMTIKMNESDAWFEEKQQQFENLDQQLRKLHVSVEALVCHRKELSANTAAFAKSAAMLGNSEDHTALSRALSQLAEVEEKIDQLHQEQAFADFYMFSELLSDYIRLIAAVKGVFDHRMKCWQKWEDAQITLLKKREAEAKMMVANKPDKIQQAKNEIREWEAKVQQGERDFEQISKTIRKEVGRFEKERVKDFKTVIIKYLESLVQTQQQLIKYWEAFLPEAKAIA 7DC07DFA523312B5 vps35_human Vacuolar protein sorting-associated protein 35 Vesicle protein sorting 35 Maternal-embryonic 3 VPS35 MEM3 TCCCTA00141 protein protein human Homo sapiens Human MPTTQQSPQDEQEKLLDEAIQAVKVQSFQMKRCLDKNKLMDALKHASNMLGELRTSMLSPKSYYELYMAISDELHYLEVYLTDEFAKGRKVADLYELVQYAGNIIPRLYLLITVGVVYVKSFPQSRKDILKDLVEMCRGVQHPLRGLFLRNYLLQCTRNILPDEGEPTDEETTGDISDSMDFVLLNFAEMNKLWVRMQHQGHSRDREKRERERQELRILVGTNLVRLSQLEGVNVERYKQIVLTGILEQVVNCRDALAQEYLMECIIQVFPDEFHLQTLNPFLRACAELHQNVNVKNIIIALIDRLALFAHREDGPGIPADIKLFDIFSQQVATVIQSRQDMPSEDVVSLQVSLINLAMKCYPDRVDYVDKVLETTVEIFNKLNLEHIATSSAVSKELTRLLKIPVDTYNNILTVLKLKHFHPLFEYFDYESRKSMSCYVLSNVLDYNTEIVSQDQVDSIMNLVSTLIQDQPDQPVEDPDPEDFADEQSLVGRFIHLLRSEDPDQQYLILNTARKHFGAGGNQRIRFTLPPLVFAAYQLAFRYKENSKVDDKWEKKCQKIFSFAHQTISALIKAELAELPLRLFLQGALAAGEIGFENHETVAYEFMSQAFSLYEDEISDSKAQLAAITLIIGTFERMKCFSEENHEPLRTQCALAASKLLKKPDQGRAVSTCAHLFWSGRNTDKNGEELHGGKRVMECLKKALKIANQCMDPSLQVQLFIEILNRYIYFYEKENDAVTIQVLNQLIQKIREDLPNLESSEETEQINKHFHNTLEHLRLRRESPESEGPIYEGLIL DIP protein Q96QK1 original sequence version: 118 DIP protein Q96QK1 original sequence version: 44 DIP protein Q96QK1 original sequence version: 117 DIP protein Q96QK1 original sequence version: 127 28D2DD1C6B920A0A mpri_human Cation-independent mannose-6-phosphate receptor Insulin-like growth factor 2 receptor Insulin-like growth factor II receptor M6P/IGF2 receptor 300 kDa mannose 6-phosphate receptor IGF2R MPRI protein protein human Homo sapiens Human MGAAAGRSPHLGPAPARRPQRSLLLLQLLLLVAAPGSTQAQAAPFPELCSYTWEAVDTKNNVLYKINICGSVDIVQCGPSSAVCMHDLKTRTYHSVGDSVLRSATRSLLEFNTTVSCDQQGTNHRVQSSIAFLCGKTLGTPEFVTATECVHYFEWRTTAACKKDIFKANKEVPCYVFDEELRKHDLNPLIKLSGAYLVDDSDPDTSLFINVCRDIDTLRDPGSQLRACPPGTAACLVRGHQAFDVGQPRDGLKLVRKDRLVLSYVREEAGKLDFCDGHSPAVTITFVCPSERREGTIPKLTAKSNCRYEIEWITEYACHRDYLESKTCSLSGEQQDVSIDLTPLAQSGGSSYISDGKEYLFYLNVCGETEIQFCNKKQAAVCQVKKSDTSQVKAAGRYHNQTLRYSDGDLTLIYFGGDECSSGFQRMSVINFECNKTAGNDGKGTPVFTGEVDCTYFFTWDTEYACVKEKEDLLCGATDGKKRYDLSALVRHAEPEQNWEAVDGSQTETEKKHFFINICHRVLQEGKARGCPEDAAVCAVDKNGSKNLGKFISSPMKEKGNIQLSYSDGDDCGHGKKIKTNITLVCKPGDLESAPVLRTSGEGGCFYEFEWHTAAACVLSKTEGENCTVFDSQAGFSFDLSPLTKKNGAYKVETKKYDFYINVCGPVSVSPCQPDSGACQVAKSDEKTWNLGLSNAKLSYYDGMIQLNYRGGTPYNNERHTPRATLITFLCDRDAGVGFPEYQEEDNSTYNFRWYTSYACPEEPLECVVTDPSTLEQYDLSSLAKSEGGLGGNWYAMDNSGEHVTWRKYYINVCRPLNPVPGCNRYASACQMKYEKDQGSFTEVVSISNLGMAKTGPVVEDSGSLLLEYVNGSACTTSDGRQTTYTTRIHLVCSRGRLNSHPIFSLNWECVVSFLWNTEAACPIQTTTDTDQACSIRDPNSGFVFNLNPLNSSQGYNVSGIGKIFMFNVCGTMPVCGTILGKPASGCEAETQTEELKNWKPARPVGIEKSLQLSTEGFITLTYKGPLSAKGTADAFIVRFVCNDDVYSGPLKFLHQDIDSGQGIRNTYFEFETALACVPSPVDCQVTDLAGNEYDLTGLSTVRKPWTAVDTSVDGRKRTFYLSVCNPLPYIPGCQGSAVGSCLVSEGNSWNLGVVQMSPQAAANGSLSIMYVNGDKCGNQRFSTRITFECAQISGSPAFQLQDGCEYVFIWRTVEACPVVRVEGDNCEVKDPRHGNLYDLKPLGLNDTIVSAGEYTYYFRVCGKLSSDVCPTSDKSKVVSSCQEKREPQGFHKVAGLLTQKLTYENGLLKMNFTGGDTCHKVYQRSTAIFFYCDRGTQRPVFLKETSDCSYLFEWRTQYACPPFDLTECSFKDGAGNSFDLSSLSRYSDNWEAITGTGDPEHYLINVCKSLAPQAGTEPCPPEAAACLLGGSKPVNLGRVRDGPQWRDGIIVLKYVDGDLCPDGIRKKSTTIRFTCSESQVNSRPMFISAVEDCEYTFAWPTATACPMKSNEHDDCQVTNPSTGHLFDLSSLSGRAGFTAAYSEKGLVYMSICGENENCPPGVGACFGQTRISVGKANKRLRYVDQVLQLVYKDGSPCPSKSGLSYKSVISFVCRPEARPTNRPMLISLDKQTCTLFFSWHTPLACEQATECSVRNGSSIVDLSPLIHRTGGYEAYDESEDDASDTNPDFYINICQPLNPMHGVPCPAGAAVCKVPIDGPPIDIGRVAGPPILNPIANEIYLNFESSTPCLADKHFNYTSLIAFHCKRGVSMGTPKLLRTSECDFVFEWETPVVCPDEVRMDGCTLTDEQLLYSFNLSSLSTSTFKVTRDSRTYSVGVCTFAVGPEQGGCKDGGVCLLSGTKGASFGRLQSMKLDYRHQDEAVVLSYVNGDRCPPETDDGVPCVFPFIFNGKSYEECIIESRAKLWCSTTADYDRDHEWGFCRHSNSYRTSSIIFKCDEDEDIGRPQVFSEVRGCDVTFEWKTKVVCPPKKLECKFVQKHKTYDLRLLSSLTGSWSLVHNGVSYYINLCQKIYKGPLGCSERASICRRTTTGDVQVLGLVHTQKLGVIGDKVVVTYSKGYPCGGNKTASSVIELTCTKTVGRPAFKRFDIDSCTYYFSWDSRAACAVKPQEVQMVNGTITNPINGKSFSLGDIYFKLFRASGDMRTNGDNYLYEIQLSSITSSRNPACSGANICQVKPNDQHFSRKVGTSDKTKYYLQDGDLDVVFASSSKCGKDKTKSVSSTIFFHCDPLVEDGIPEFSHETADCQYLFSWYTSAVCPLGVGFDSENPGDDGQMHKGLSERSQAVGAVLSLLLVALTCCLLALLLYKKERRETVISKLTTCCRRSSNVSYKYSKVNKEEETDENETEWLMEEIQLPPPRQGKEGQENGHITTKSVKALSSLHGDDQDSEDEVLTIPEVKVHSGRGAGAESSHPVRNAQSNALQEREDDRVGLVRGEKARKGKSSSAQQKTVSSTKLVSFHDDSDEDLLHI DIP protein P11717 original sequence version: 92 3841ADE559B48057 vp26a_human Vacuolar protein sorting-associated protein 26A VPS26A VPS26 Vesicle protein sorting 26A protein protein human Homo sapiens Human MSFLGGFFGPICEIDIVLNDGETRKMAEMKTEDGKVEKHYLFYDGESVSGKVNLAFKQPGKRLEHQGIRIEFVGQIELFNDKSNTHEFVNLVKELALPGELTQSRSYDFEFMQVEKPYESYIGANVRLRYFLKVTIVRRLTDLVKEYDLIVHQLATYPDVNNSIKMEVGIEDCLHIEFEYNKSKYHLKDVIVGKIYFLLVRIKIQHMELQLIKKEITGIGPSTTTETETIAKYEIMDGAPVKGESIPIRLFLAGYDPTPTMRDVNKKFSVRYFLNLVLVDEEDRRYFKQQEIILWRKAPEKLRKQRTNFHQRFESPESQASAEQPEM DIP protein O75436 original sequence version: 115 DIP protein O75436 original sequence version: 53 DIP protein O75436 original sequence version: 113 DIP protein O75436-1 original sequence version: 113 DIP protein O75436 original sequence version: 123 BD3B2EAA6CFCBFA9 vps35-igf2r 2 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 1C vps35-igf2r-1 2 7 unspecified role unspecified role prey prey cytoplasmic_domain sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 1D snx2-vps26a-1 4 5 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role neutral component neutral component colocalization colocalization 2I igf2r-vps26a 4 7 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization 3O igf2r-vps26a-1 3 7 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization 5B vps26a-vps35-3 1 6 tag fluorescence tag visualisation by fluorescence tag fluorescence immunostaining immunostaining unspecified role unspecified role neutral component neutral component nucl transfection nucleic acid transfection nucl transfection n_terminus yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role neutral component neutral component colocalization colocalization 2L