IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. Dev. Cell (1534-5807) 2002 imex curation Mishima M., Kaitna S., Glotzer M. mglotzer@nt.imp.univie.ac.at 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro electrophoresis electrophoretic mobility-based method electrophoresis weight by staining molecular weight estimation by staining weight by staining Mishima M., Kaitna S., Glotzer M. mglotzer@nt.imp.univie.ac.at Accepted 2014-JUL-18 by MGT Dev. Cell (1534-5807) 2002 Only protein-protein interactions imex curation Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. Dev. Cell (1534-5807) 2002 imex curation Mishima M., Kaitna S., Glotzer M. mglotzer@nt.imp.univie.ac.at 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions caeel-embryo Caenorhabditis elegans embryo embryo Embryo Defined as the development stage prior to the initiation of tissue differentiation. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mishima M., Kaitna S., Glotzer M. mglotzer@nt.imp.univie.ac.at Accepted 2014-JUL-09 by MGT Dev. Cell (1534-5807) 2002 Only protein-protein interactions imex curation Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. Dev. Cell (1534-5807) 2002 imex curation Mishima M., Kaitna S., Glotzer M. mglotzer@nt.imp.univie.ac.at 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Mishima M., Kaitna S., Glotzer M. mglotzer@nt.imp.univie.ac.at Accepted 2014-JUL-09 by MGT Dev. Cell (1534-5807) 2002 Only protein-protein interactions imex curation Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. Dev. Cell (1534-5807) 2002 imex curation Mishima M., Kaitna S., Glotzer M. mglotzer@nt.imp.univie.ac.at 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging anti-tag immunocytochemistry anti-tag immunocytochemistry Mishima M., Kaitna S., Glotzer M. mglotzer@nt.imp.univie.ac.at Accepted 2014-JUL-09 by MGT Dev. Cell (1534-5807) 2002 Only protein-protein interactions imex curation Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. Dev. Cell (1534-5807) 2002 imex curation Mishima M., Kaitna S., Glotzer M. mglotzer@nt.imp.univie.ac.at 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro anti bait coip anti bait coimmunoprecipitation anti bait coip weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Mishima M., Kaitna S., Glotzer M. Its not clear for the coip expts, when the proteins are tagged and when not - they make both - so I have assumed not unless the tag is stated. mglotzer@nt.imp.univie.ac.at Accepted 2014-JUL-09 by MGT Dev. Cell (1534-5807) 2002 Only protein-protein interactions imex curation Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. Dev. Cell (1534-5807) 2002 imex curation Mishima M., Kaitna S., Glotzer M. mglotzer@nt.imp.univie.ac.at 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip sequence tag sequence tag identification sequence tag Mishima M., Kaitna S., Glotzer M. mglotzer@nt.imp.univie.ac.at Accepted 2014-JUL-18 by MGT Dev. Cell (1534-5807) 2002 Only protein-protein interactions imex curation Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. Central spindle assembly and cytokinesis require a kinesin-like protein/RhoGAP complex with microtubule bundling activity. Dev. Cell (1534-5807) 2002 imex curation Mishima M., Kaitna S., Glotzer M. mglotzer@nt.imp.univie.ac.at 18-Sep-2014: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 density sedimentation cosedimentation through density gradient density sedimentation western blot western blot Immuno blot Immuno blot Mishima M., Kaitna S., Glotzer M. mglotzer@nt.imp.univie.ac.at Accepted 2014-JUL-09 by MGT Dev. Cell (1534-5807) 2002 Only protein-protein interactions imex curation kif23_human Kinesin-like protein KIF23 KIF23 KNSL5 MKLP1 Mitotic kinesin-like protein 1 Kinesin-like protein 5 protein protein human Homo sapiens Human MKSARAKTPRKPTVKKGSQTNLKDPVGVYCRVRPLGFPDQECCIEVINNTTVQLHTPEGYRLNRNGDYKETQYSFKQVFGTHTTQKELFDVVANPLVNDLIHGKNGLLFTYGVTGSGKTHTMTGSPGEGGLLPRCLDMIFNSIGSFQAKRYVFKSNDRNSMDIQCEVDALLERQKREAMPNPKTSSSKRQVDPEFADMITVQEFCKAEEVDEDSVYGVFVSYIEIYNNYIYDLLEEVPFDPIKPKPPQSKLLREDKNHNMYVAGCTEVEVKSTEEAFEVFWRGQKKRRIANTHLNRESSRSHSVFNIKLVQAPLDADGDNVLQEKEQITISQLSLVDLAGSERTNRTRAEGNRLREAGNINQSLMTLRTCMDVLRENQMYGTNKMVPYRDSKLTHLFKNYFDGEGKVRMIVCVNPKAEDYEENLQVMRFAEVTQEVEVARPVDKAICGLTPGRRYRNQPRGPVGNEPLVTDVVLQSFPPLPSCEILDINDEQTLPRLIEALEKRHNLRQMMIDEFNKQSNAFKALLQEFDNAVLSKENHMQGKLNEKEKMISGQKLEIERLEKKNKTLEYKIEILEKTTTIYEEDKRNLQQELETQNQKLQRQFSDKRRLEARLQGMVTETTMKWEKECERRVAAKQLEMQNKLWVKDEKLKQLKAIVTEPKTEKPERPSRERDREKVTQRSVSPSPVPLSSNYIAQISNGQQLMSQPQLHRRSNSCSSISVASCISEWEQKIPTYNTPLKVTSIARRRQQEPGQSKTCIVSDRRRGMYWTEGREVVPTFRNEIEIEEDHCGRLLFQPDQNAPPIRLRHRRSRSAGDRWVDHKPASNMQTETVMQPHVPHAITVSVANEKALAKCEKYMLTHQELASDGEIETKLIKGDIYKTRGGGQSVQFTDIETLKQESPNGSRKRRSSTVAPAQPDGAESEWTDVETRCSVAVEMRAGSQLGPGYQHHAQPKRKKP DIP protein Q02241 original sequence version: 104 DIP protein Q02241 original sequence version: 159 DIP protein Q02241 original sequence version: 160 B2AA784D2D236A90 q8mq03_caeel Kinesin-like protein zen-4 CELE_M03D4.1 M03D4.1 protein protein caeel Caenorhabditis elegans MSSRKRGITPSRDQVRRKKLSIEETDSIEVVCRLCPYTGSTPSLIAIDEGSIQTVLPPAQFRRENAPQVEKVFRFGRVFSENDGQATVFERTSVDLILNLLKGQNSLLFTYGVTGSGKTYTMTGKPTETGTGLLPRTLDVIFNSINNRVEKCIFYPSALNTFEIRATLDAHLKRHQMAADRLSTSREITDRYCEAIKLSGYNDDMVCSVFVTYVEIYNNYCYDLLEDARNGSRVLTKREIRHDRQQQMYVDGAKDVEVSSSEEALEVFCLGEERRRVSSTLLNKDSSRSHSVFTIKLVMAPRAYETKSVYPTMDSSQIIVSQLCLVDLAGSERAKRTQNVGERLAEANSINQSLMTLRQCIEVLRRNQKSSSQNLEQVPYRQSKLTHLFKNYLEGNGKIRMVICVNPKPDDYDENMSALAFAEESQTIEVKKQVERMPSERIPHSFFTQWNSELDGSVRMEDDGSREIPCPPTFCLTDCNDKDTVDSMYKYARKLSSLQNSSEEGPSSTLLTMIRQYMMEADYQRVEIARLKDSLNDKDEEIKKLRGFCSRYKRENASMKERIASCEQGEQENALVMEKLMEQKMEDRKIIQSQKKAMRNVRGIIDNPSPSVASLRSRFDQENVAHPTAPIQTPPPPYQTPGRAPVFKKRLEATTSTTVMSGSSSGGSGQQGYVNPKYQRRSKSASRLLDHQPLHRVPTGTVLQSRTPANAIRTTKPEMHQLNKSGEYRLTHQEVDDEGNISTNIVKAPSKPPELQLEDSQSSSYSPCTDVKSITIYSNHDVEEEDE 10577582647B4EC5 rgap1_human Rac GTPase-activating protein 1 Male germ cell RacGap RACGAP1 KIAA1478 MGCRACGAP Protein CYK4 homolog protein protein human Homo sapiens Human MDTMMLNVRNLFEQLVRRVEILSEGNEVQFIQLAKDFEDFRKKWQRTDHELGKYKDLLMKAETERSALDVKLKHARNQVDVEIKRRQRAEADCEKLERQIQLIREMLMCDTSGSIQLSEEQKSALAFLNRGQPSSSNAGNKRLSTIDESGSILSDISFDKTDESLDWDSSLVKTFKLKKREKRRSTSRQFVDGPPGPVKKTRSIGSAVDQGNESIVAKTTVTVPNDGGPIEAVSTIETVPYWTRSRRKTGTLQPWNSDSTLNSRQLEPRTETDSVGTPQSNGGMRLHDFVSKTVIKPESCVPCGKRIKFGKLSLKCRDCRVVSHPECRDRCPLPCIPTLIGTPVKIGEGMLADFVSQTSPMIPSIVVHCVNEIEQRGLTETGLYRISGCDRTVKELKEKFLRVKTVPLLSKVDDIHAICSLLKDFLRNLKEPLLTFRLNRAFMEAAEITDEDNSIAAMYQAVGELPQANRDTLAFLMIHLQRVAQSPHTKMDVANLAKVFGPTIVAHAVPNPDPVTMLQDIKRQPKVVERLLSLPLEYWSQFMMVEQENIDPLHVIENSNAFSTPQTPDIKVSLLGPVTTPEHQLLKTPSSSSLSQRVRSTLTKNTPRFGSKSKSATNLGRQGNFFASPMLK DIP protein Q9H0H5 original sequence version: 87 DIP protein Q9H0H5 original sequence version: 144 032B7DF9CEA8F39D q9xus9_caeel cyk-4 CELE_K08E3.6 K08E3.6 protein protein caeel Caenorhabditis elegans MKSSTSKEKVCGENSRHIFNMILNSQRPQFDIKDIGMFHLIDEIERLRKLWKDSEESKKRLNADMREAEEALAKARKKLAMFDIDVKDTQKHLRALMEENKALKLDLNVYETREKQLKDAMKNGIFNSLTKEDRDQFKFLHEPLVRTYSKRVQQRHPHLMEDTQDDEDDSEVDYDETGDSFEEVIHLRNGREVRRSSAAGNAVGGKRRSASAHAITAAANSKRSRSRVMTATIDEEPNEGGTPPKRCRDDGSTPHQEMTTTTTTTTTTTIHNSRAQNQDPPRVSLHRQLTRRSLSCGSIPSCDQTPGQTTNNIGLGMSSAILTKSTLDIRTLKRGTPAWTNGTTRDIAMRPHTFIEAGIKAMRKCDKCATALKLATSMKCRDCHQVVHRSCCNKLHLPCIPRPKTMMTPKSALRGAKPGAGEFRLQDFCTSAKPMIPAAVIHCVVALEARGLTQEGIYRVPGQVRTVNVLLDELRSKTVPNVGLHDVEVITDTLKRFLRDLKDPLIPRTSRQELIVAANLYSTDPDNGRLALNRVICELPQANRDTLAYLFIHWRKVIAQSSRNKMNCEAMARMVAPAVMGHPVKQSQSQAIAGRDATDCHRAMTALFEFDDVYWQRFLGTSAVSMASNQIETARHQDNFALCDRSILGPVTTSPATPLLARSANATRARGAHLLGSMFHD DIP protein Q9XUS9 original sequence version: 105 B3DD74F4CC51B685 cyk_4-zen_4 2 11 unspecified role unspecified role bait bait 9 unspecified role unspecified role prey prey physical association physical association Fig. 1A zen_4-cyk_4-1 5 11 unspecified role unspecified role prey prey Q9XUS9:p.Ser15Leu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain S L Wed May 02 15:56:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser15leu' Tue Jun 30 19:19:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser15Leu' region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 9 unspecified role unspecified role bait bait in vitro In vitro physical association physical association Fig. 2A/E cyk_4-zen_4-2 5 11 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined in vitro In vitro 9 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n_terminal_chitin binding tag tag tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range in vitro In vitro physical association physical association Fig. 1C cyk_4-zen_4-4 5 11 unspecified role unspecified role bait bait in vitro In vitro 9 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro physical association physical association Fig. 2C zen_4-cyk_4-3 5 9 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 11 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain Q9XUS9:p.Ser15Leu mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain S L Wed May 02 14:33:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser15leu' Tue Jun 30 16:00:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser15Leu' in vitro In vitro 34 37 physical association physical association Fig. 3B The suppressor mutation (E502K) in ZEN-4 partially recovered the interaction of the CYK-4 S15L mutant with ZEN-4 (z4). cyk_4-cyk_4 5 11 unspecified role unspecified role bait bait region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 11 unspecified role unspecified role prey prey region 35s radiolabel 35s radiolabel 35S s35 radiolabelled S35 s35 radiolabelled 35S S35 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain in vitro In vitro 45 42 physical association physical association 4A racgap1-kif23 3 10 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association Fig. 5A kif23-racgap1-3 3 8 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association Fig. 5A kif23-racgap1-4 4 8 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 5B In early anaphase, KIF23 and RACGAP1 localize to the nascent central spindle. During mid-to-late anaphase they localize to short segments in the central spindle kif23-racgap1-5 7 10 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 5D kif23-racgap1-7 6 10 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait physical association physical association Fig. 5E The complex was immunoprecipitated from a microtubule binding fraction of mitotic HeLa cells racgap1-kif23-1 6 10 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association Fig. 5E The complex was immunoprecipitated from a microtubule binding fraction of mitotic HeLa cells cyk_4-zen_4-7 1 9 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection 11 unspecified role unspecified role neutral component neutral component spofr-sf_9 Spodoptera frugiperda insect cells Fall armyworm sf_9 insect cells Derived from pupal ovarian tissue of the fall armyworm Cloned from the parent cell line, IPLB-SF-21-AE (SF-21) derived from immature ovaries of fall armyworm Spodoptera frugiperda pupae cells are susceptible to Baculovirus infection direct interaction direct interaction Fig. 6A