IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ PDGF-D, a new protease-activated growth factor. PDGF-D, a new protease-activated growth factor. Nat. Cell Biol. (1465-7392) 2001 imex curation LaRochelle W.J., Jeffers M., McDonald W.F., Chillakuru R.A., Giese N.A., Lokker N.A., Sullivan C., Boldog F.L., Yang M., Vernet C., Burgess C.E., Fernandes E., Deegler L.L., Rittman B., Shimkets J., Shimkets R.A., Rothberg J.M., Lichenstein H.S. wlarochelle@curagen.com 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions human-ccd-1070sk CCD-1070Sk human foreskin fibroblasts ccd-1070sk CCD-1070Sk human foreskin fibroblasts ATCC Cat. No: ATCC® CRL-2091™ http://www.lgcstandards-atcc.org/products/all/CRL-2091.aspx?geo_country=gb#generalinformation elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection LaRochelle W.J., Jeffers M., McDonald W.F., Chillakuru R.A., Giese N.A., Lokker N.A., Sullivan C., Boldog F.L., Yang M., Vernet C., Burgess C.E., Fernandes E., Deegler L.L., Rittman B., Shimkets J., Shimkets R.A., Rothberg J.M., Lichenstein H.S. wlarochelle@curagen.com Accepted 2014-JAN-31 by PPORRAS Nat. Cell Biol. (1465-7392) 2001 Only protein-protein interactions imex curation PDGF-D, a new protease-activated growth factor. PDGF-D, a new protease-activated growth factor. Nat. Cell Biol. (1465-7392) 2001 imex curation LaRochelle W.J., Jeffers M., McDonald W.F., Chillakuru R.A., Giese N.A., Lokker N.A., Sullivan C., Boldog F.L., Yang M., Vernet C., Burgess C.E., Fernandes E., Deegler L.L., Rittman B., Shimkets J., Shimkets R.A., Rothberg J.M., Lichenstein H.S. wlarochelle@curagen.com 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro comigration in sds comigration in sds page comigration in sds weight by comassie molecular weight estimation by coomasie staining weight by comassie LaRochelle W.J., Jeffers M., McDonald W.F., Chillakuru R.A., Giese N.A., Lokker N.A., Sullivan C., Boldog F.L., Yang M., Vernet C., Burgess C.E., Fernandes E., Deegler L.L., Rittman B., Shimkets J., Shimkets R.A., Rothberg J.M., Lichenstein H.S. wlarochelle@curagen.com Accepted 2014-JAN-31 by PPORRAS Nat. Cell Biol. (1465-7392) 2001 Only protein-protein interactions imex curation q9gzp0-pro_0000250188 Platelet-derived growth factor D, latent form PDGFD IEGF SCDGFB Iris-expressed growth factor Spinal cord-derived growth factor B MSTP036 UNQ1899/PRO4345 protein protein human Homo sapiens Human RDTSATPQSASIKALRNANLRRDESNHLTDLYRRDETIQVKGNGYVQSPRFPNSYPRNLLLTWRLHSQENTRIQLVFDNQFGLEEAENDICRYDFVEVEDISETSTIIRGRWCGHKEVPPRIKSRTNQIKITFKSDDYFVAKPGFKIYYSLLEDFQPAAASETNWESVTSSISGVSYNSPSVTDPTLIADALDKKIAEFDTVEDLLKYFNPESWQEDLENMYLDTPRYRGRSYHDRKSKVDLDRLNDDAKRYSCTPRNYSVNIREELKLANVVFFPRCLLVQRCGGNCGCGTVNWRSCTCNSGKTVKKYHEVLQFEPGHIKRRGRAKTMALVDIQLDHHERCDCICSSRPPR 19 370 44E99628D85DD348 pgfra_human Platelet-derived growth factor receptor alpha PDGFRA CD140 antigen-like family member A CD140a antigen Alpha-type platelet-derived growth factor receptor Alpha platelet-derived growth factor receptor Platelet-derived growth factor receptor 2 RHEPDGFRA PDGFR2 Platelet-derived growth factor alpha receptor protein protein human Homo sapiens Human MGTSHPAFLVLGCLLTGLSLILCQLSLPSILPNENEKVVQLNSSFSLRCFGESEVSWQYPMSEEESSDVEIRNEENNSGLFVTVLEVSSASAAHTGLYTCYYNHTQTEENELEGRHIYIYVPDPDVAFVPLGMTDYLVIVEDDDSAIIPCRTTDPETPVTLHNSEGVVPASYDSRQGFNGTFTVGPYICEATVKGKKFQTIPFNVYALKATSELDLEMEALKTVYKSGETIVVTCAVFNNEVVDLQWTYPGEVKGKGITMLEEIKVPSIKLVYTLTVPEATVKDSGDYECAARQATREVKEMKKVTISVHEKGFIEIKPTFSQLEAVNLHEVKHFVVEVRAYPPPRISWLKNNLTLIENLTEITTDVEKIQEIRYRSKLKLIRAKEEDSGHYTIVAQNEDAVKSYTFELLTQVPSSILDLVDDHHGSTGGQTVRCTAEGTPLPDIEWMICKDIKKCNNETSWTILANNVSNIITEIHSRDRSTVEGRVTFAKVEETIAVRCLAKNLLGAENRELKLVAPTLRSELTVAAAVLVLLVIVIISLIVLVVIWKQKPRYEIRWRVIESISPDGHEYIYVDPMQLPYDSRWEFPRDGLVLGRVLGSGAFGKVVEGTAYGLSRSQPVMKVAVKMLKPTARSSEKQALMSELKIMTHLGPHLNIVNLLGACTKSGPIYIITEYCFYGDLVNYLHKNRDSFLSHHPEKPKKELDIFGLNPADESTRSYVILSFENNGDYMDMKQADTTQYVPMLERKEVSKYSDIQRSLYDRPASYKKKSMLDSEVKNLLSDDNSEGLTLLDLLSFTYQVARGMEFLASKNCVHRDLAARNVLLAQGKIVKICDFGLARDIMHDSNYVSKGSTFLPVKWMAPESIFDNLYTTLSDVWSYGILLWEIFSLGGTPYPGMMVDSTFYNKIKSGYRMAKPDHATSEVYEIMVKCWNSEPEKRPSFYHLSEIVENLLPGQYKKSYEKIHLDFLKSDHPAVARMRVDSDNAYIGVTYKNEEDKLKDWEGGLDEQRLSADSGYIIPLPDIDPVPEEEDLGKRNRHSSQTSEESAIETGSSSSTFIKREDETIEDIDMMDDIGIDSSDLVEDSFL 5E3FB9940ACD1BE8 pgfrb_human Platelet-derived growth factor receptor beta CD140 antigen-like family member B PDGFRB Beta-type platelet-derived growth factor receptor PDGFR Platelet-derived growth factor receptor 1 Beta platelet-derived growth factor receptor PDGFR1 protein protein human Homo sapiens Human MRLPGAMPALALKGELLLLSLLLLLEPQISQGLVVTPPGPELVLNVSSTFVLTCSGSAPVVWERMSQEPPQEMAKAQDGTFSSVLTLTNLTGLDTGEYFCTHNDSRGLETDERKRLYIFVPDPTVGFLPNDAEELFIFLTEITEITIPCRVTDPQLVVTLHEKKGDVALPVPYDHQRGFSGIFEDRSYICKTTIGDREVDSDAYYVYRLQVSSINVSVNAVQTVVRQGENITLMCIVIGNEVVNFEWTYPRKESGRLVEPVTDFLLDMPYHIRSILHIPSAELEDSGTYTCNVTESVNDHQDEKAINITVVESGYVRLLGEVGTLQFAELHRSRTLQVVFEAYPPPTVLWFKDNRTLGDSSAGEIALSTRNVSETRYVSELTLVRVKVAEAGHYTMRAFHEDAEVQLSFQLQINVPVRVLELSESHPDSGEQTVRCRGRGMPQPNIIWSACRDLKRCPRELPPTLLGNSSEEESQLETNVTYWEEEQEFEVVSTLRLQHVDRPLSVRCTLRNAVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQKKPRYEIRWKVIESVSSDGHEYIYVDPMQLPYDSTWELPRDQLVLGRTLGSGAFGQVVEATAHGLSHSQATMKVAVKMLKSTARSSEKQALMSELKIMSHLGPHLNVVNLLGACTKGGPIYIITEYCRYGDLVDYLHRNKHTFLQHHSDKRRPPSAELYSNALPVGLPLPSHVSLTGESDGGYMDMSKDESVDYVPMLDMKGDVKYADIESSNYMAPYDNYVPSAPERTCRATLINESPVLSYMDLVGFSYQVANGMEFLASKNCVHRDLAARNVLICEGKLVKICDFGLARDIMRDSNYISKGSTFLPLKWMAPESIFNSLYTTLSDVWSFGILLWEIFTLGGTPYPELPMNEQFYNAIKRGYRMAQPAHASDEIYEIMQKCWEEKFEIRPPFSQLVLLLERLLGEGYKKKYQQVDEEFLRSDHPAILRSQARLPGFHGLRSPLDTSSVLYTAVQPNEGDNDYIIPLPDPKPEVADEGPLEGSPSLASSTLNEVNTSSTISCDSPLEPQDEPEPEPQLELQVEPEPELEQLPDSGCPAPRAEAEDSFL DIP protein P09619 original sequence version: 176 DIP protein P09619 original sequence version: 185 DIP protein P09619 original sequence version: 114 DIP protein P09619 original sequence version: 162 DIP protein P09619 original sequence version: 150 DIP protein P09619 original sequence version: 165 DIP protein P09619 original sequence version: 161 038C15E531D6E89D q9gzp0-pro_0000250189 Platelet-derived growth factor D, receptor-binding form PDGFD IEGF SCDGFB Iris-expressed growth factor Spinal cord-derived growth factor B MSTP036 UNQ1899/PRO4345 protein protein human Homo sapiens Human SYHDRKSKVDLDRLNDDAKRYSCTPRNYSVNIREELKLANVVFFPRCLLVQRCGGNCGCGTVNWRSCTCNSGKTVKKYHEVLQFEPGHIKRRGRAKTMALVDIQLDHHERCDCICSSRPPR 250 370 BF18456A22B49776 pdgfd 2 3 unspecified role unspecified role neutral component neutral component human-293 ebna Homo sapiens 293 ebna 293 ebna direct interaction direct interaction Fig. 2b Proteolytic cleavage of the n-terminus was achieved by expressing PDGF-D in HEK 293 cells without FBS in medium. pdgfrb-pdgfd 1 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait proximity proximity close-contact colocalization neighbourhood interaction Fig. 3i This interaction is activated by the addition of the homodimeric PDGF-BB or PDGF-DD ligand. PDGF-DD activation is much weaker than PDGF-BB. pdgfd-1 2 6 unspecified role unspecified role neutral component neutral component human-293 ebna Homo sapiens 293 ebna 293 ebna direct interaction direct interaction Fig. 2b Proteolytic cleavage was achieved by expressing PDGF-D in HEK 293 cells in FBS-containing medium. Cleavage was either after Arg247 or Arg249.