IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The hydrophilic isoform of glutamate decarboxylase, GAD67, is targeted to membranes and nerve terminals independent of dimerization with the hydrophobic membrane-anchored isoform, GAD65. The hydrophilic isoform of glutamate decarboxylase, GAD67, is targeted to membranes and nerve terminals independent of dimerization with the hydrophobic membrane-anchored isoform, GAD65. J. Biol. Chem. (0021-9258) 1999 imex curation Kanaani J., Lissin D., Kash S.F., Baekkeskov S. 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions rat-brain-hippocampus Hippocampus of rat brain rat-brain-hippocampus Hippocampus of rat brain fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Kanaani J., Lissin D., Kash S.F., Baekkeskov S. Accepted 2014-MAY-14 by ORCHARD J. Biol. Chem. (0021-9258) 1999 Only protein-protein interactions imex curation The hydrophilic isoform of glutamate decarboxylase, GAD67, is targeted to membranes and nerve terminals independent of dimerization with the hydrophobic membrane-anchored isoform, GAD65. The hydrophilic isoform of glutamate decarboxylase, GAD67, is targeted to membranes and nerve terminals independent of dimerization with the hydrophobic membrane-anchored isoform, GAD65. J. Biol. Chem. (0021-9258) 1999 imex curation Kanaani J., Lissin D., Kash S.F., Baekkeskov S. 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions rat-brain Rattus norvegicus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kanaani J., Lissin D., Kash S.F., Baekkeskov S. Host tissue is mixture of cerebellum and cerebral cortex. Accepted 2014-MAY-14 by ORCHARD J. Biol. Chem. (0021-9258) 1999 Only protein-protein interactions imex curation The hydrophilic isoform of glutamate decarboxylase, GAD67, is targeted to membranes and nerve terminals independent of dimerization with the hydrophobic membrane-anchored isoform, GAD65. The hydrophilic isoform of glutamate decarboxylase, GAD67, is targeted to membranes and nerve terminals independent of dimerization with the hydrophobic membrane-anchored isoform, GAD65. J. Biol. Chem. (0021-9258) 1999 imex curation Kanaani J., Lissin D., Kash S.F., Baekkeskov S. 11-Jun-2014: Contacted by IntAct-Help. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kanaani J., Lissin D., Kash S.F., Baekkeskov S. The deletion mutant GAD65del1-38 also has a mutation Cys45Ala that prevents palmitoylation. Accepted 2014-MAY-14 by ORCHARD J. Biol. Chem. (0021-9258) 1999 Only protein-protein interactions imex curation dce2_mouse Glutamate decarboxylase 2 Gad2 Gad65 Glutamate decarboxylase 65 kDa isoform 65 kDa glutamic acid decarboxylase protein protein mouse Mus musculus Mouse MASPGSGFWSFGSEDGSADPENPGTARAWCQVAQKFTGGIGNKLCALLYGDSGKPAEGGGSVTSRAATGKVACTCDQKPCNCPKGDVNYAFLHATDLLPACDGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWELADQPQNLEEILTHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWLTSTANTNMFTYEIAPVFVLLEYVTLKKMREIIGWPGGSGDGIFSPGGAISNMYAMLIARYKMFPEVKEKGMAAVPRLIAFTSEHSHFSLKKGAAALGIGTDSVILIKCDERGKMIPSDLERRILEVKQKGFVPFLVSATAGTTVYGAFDPLLAVADICKKYKIWMHVDAAWGGGLLMSRKHKWKLSGVERANSVTWNPHKMMGVPLQCSALLVREEGLMQSCNQMHASYLFQQDKHYDLSYDTGDKALQCGRHVDVFKLWLMWRAKGTTGFEAHIDKCLELAEYLYTIIKNREGYEMVFDGKPQHTNVCFWFVPPSLRTLEDNEERMSRLSKVAPVIKARMMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL Catalyzes the production of GABA mint C2F486E85123B057 dce1_rat Glutamate decarboxylase 1 Gad1 Gad67 67 kDa glutamic acid decarboxylase Glutamate decarboxylase 67 kDa isoform protein protein rat Rattus norvegicus (Rat) Rat MASSTPSPATSSNAGADPNTTNLRPTTYDTWCGVAHGCTRKLGLKICGFLQRTNSLEEKSRLVSAFRERQASKNLLSCENSDPGARFRRTETDFSNLFAQDLLPAKNGEEQTVQFLLEVVDILLNYVRKTFDRSTKVLDFHHPHQLLEGMEGFNLELSDHPESLEQILVDCRDTLKYGVRTGHPRFFNQLSTGLDIIGLAGEWLTSTANTNMFTYEIAPVFVLMEQITLKKMREIIGWSNKDGDGIFSPGGAISNMYSIMAARYKYFPEVKTKGMAAVPKLVLFTSEHSHYSIKKAGAALGFGTDNVILIKCNERGKIIPADLEAKILDAKQKGFVPLYVNATAGTTVYGAFDPIQEIADICEKYNLWLHVDAAWGGGLLMSRKHRHKLSGIERANSVTWNPHKMMGVLLQCSAILVKEKGILQGCNQMCAGYLFQPDKQYDVSYDTGDKAIQCGRHVDIFKFWLMWKAKGTVGFENQINKCLELAEYLYAKIKNREEFEMVFNGEPEHTNVCFWYIPQSLRGVPDSPERREKLHRVAPKIKALMMESGTTMVGYQPQGDKANFFRMVISNPAATQSDIDFLIEEIERLGQDL EF83239C30301F69 dce2_rat Glutamate decarboxylase 2 Gad2 Gad65 65 kDa glutamic acid decarboxylase Glutamate decarboxylase 65 kDa isoform protein protein rat Rattus norvegicus (Rat) Rat MASPGSGFWSFGSEDGSGDPENPGTARAWCQVAQKFTGGIGNKLCALLYGDSEKPAESGGSVTSRAATRKVACTCDQKPCSCPKGDVNYALLHATDLLPACEGERPTLAFLQDVMNILLQYVVKSFDRSTKVIDFHYPNELLQEYNWELADQPQNLEEILTHCQTTLKYAIKTGHPRYFNQLSTGLDMVGLAADWLTSTANTNMFTYEIAPVFVLLEYVTLKKMREIIGWPGGSGDGIFSPGGAISNMYAMLIARYKMFPEVKEKGMAAVPRLIAFTSEHSHFSLKKGAAALGIGTDSVILIKCDERGKMIPSDLERRILEVKQKGFVPFLVSATAGTTVYGAFDPLLAVADICKKYKIWMHVDAAWGGGLLMSRKHKWKLNGVERANSVTWNPHKMMGVPLQCSALLVREEGLMQSCNQMHASYLFQQDKHYDLSYDTGDKALQCGRHVDVFKLWLMWRAKGTTGFEAHIDKCLELAEYLYNIIKNREGYEMVFDGKPQHTNVCFWFVPPSLRVLEDNEERMSRLSKVAPVIKARMMEYGTTMVSYQPLGDKVNFFRMVISNPAATHQDIDFLIEEIERLGQDL C04040B7BA7B37D1 gad1-gad1 3 5 unspecified role unspecified role prey prey 39-593 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P18088:p.Lys45Ala mutation mutation certain certain sequence position certain certain certain sequence position certain K A Wed May 02 20:18:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys45ala' Tue Jun 30 16:50:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys45Ala' 5 unspecified role unspecified role bait bait physical association physical association Fig. 2a gad1-gad2-1 3 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait 102-593 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 3b, lanes 6 & 10 gad1-gad2-2 2 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association Fig. 3a The interaction is detected in both the cytosolic and membrane fraction. gad1-gad2-6 1 6 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 6