bhf-ucl The human delta1261 mutation of the HERG potassium channel results in a truncated protein that contains a subunit interaction domain and decreases the channel expression. The human delta1261 mutation of the HERG potassium channel results in a truncated protein that contains a subunit interaction domain and decreases the channel expression. J. Biol. Chem. (0021-9258) 1997 imex curation Li X., Xu J., Li M. min_li@qmail.bs.jhu.edu 09-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions ecolx Escherichia coli molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight silver stain molecular weight estimation by silver staining weight silver stain Li X., Xu J., Li M. min_li@qmail.bs.jhu.edu Accepted 2013-AUG-07 by ORCHARD 09-Aug-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 1997 imex curation The human delta1261 mutation of the HERG potassium channel results in a truncated protein that contains a subunit interaction domain and decreases the channel expression. The human delta1261 mutation of the HERG potassium channel results in a truncated protein that contains a subunit interaction domain and decreases the channel expression. J. Biol. Chem. (0021-9258) 1997 imex curation Li X., Xu J., Li M. min_li@qmail.bs.jhu.edu 09-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Li X., Xu J., Li M. min_li@qmail.bs.jhu.edu Accepted 2013-AUG-07 by ORCHARD 09-Aug-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 1997 imex curation The human delta1261 mutation of the HERG potassium channel results in a truncated protein that contains a subunit interaction domain and decreases the channel expression. The human delta1261 mutation of the HERG potassium channel results in a truncated protein that contains a subunit interaction domain and decreases the channel expression. J. Biol. Chem. (0021-9258) 1997 imex curation Li X., Xu J., Li M. min_li@qmail.bs.jhu.edu 09-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink weight silver stain molecular weight estimation by silver staining weight silver stain Li X., Xu J., Li M. min_li@qmail.bs.jhu.edu Accepted 2013-AUG-07 by ORCHARD 09-Aug-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 1997 imex curation kcnh2_human Potassium voltage-gated channel subfamily H member 2 Voltage-gated potassium channel subunit Kv11.1 Ether-a-go-go-related gene potassium channel 1 Eag homolog KCNH2 ERG ERG1 HERG protein protein human Homo sapiens Human MPVRRGHVAPQNTFLDTIIRKFEGQSRKFIIANARVENCAVIYCNDGFCELCGYSRAEVMQRPCTCDFLHGPRTQRRAAAQIAQALLGAEERKVEIAFYRKDGSCFLCLVDVVPVKNEDGAVIMFILNFEVVMEKDMVGSPAHDTNHRGPPTSWLAPGRAKTFRLKLPALLALTARESSVRSGGAGGAGAPGAVVVDVDLTPAAPSSESLALDEVTAMDNHVAGLGPAEERRALVGPGSPPRSAPGQLPSPRAHSLNPDASGSSCSLARTRSRESCASVRRASSADDIEAMRAGVLPPPPRHASTGAMHPLRSGLLNSTSDSDLVRYRTISKIPQITLNFVDLKGDPFLASPTSDREIIAPKIKERTHNVTEKVTQVLSLGADVLPEYKLQAPRIHRWTILHYSPFKAVWDWLILLLVIYTAVFTPYSAAFLLKETEEGPPATECGYACQPLAVVDLIVDIMFIVDILINFRTTYVNANEEVVSHPGRIAVHYFKGWFLIDMVAAIPFDLLIFGSGSEELIGLLKTARLLRLVRVARKLDRYSEYGAAVLFLLMCTFALIAHWLACIWYAIGNMEQPHMDSRIGWLHNLGDQIGKPYNSSGLGGPSIKDKYVTALYFTFSSLTSVGFGNVSPNTNSEKIFSICVMLIGSLMYASIFGNVSAIIQRLYSGTARYHTQMLRVREFIRFHQIPNPLRQRLEEYFQHAWSYTNGIDMNAVLKGFPECLQADICLHLNRSLLQHCKPFRGATKGCLRALAMKFKTTHAPPGDTLVHAGDLLTALYFISRGSIEILRGDVVVAILGKNDIFGEPLNLYARPGKSNGDVRALTYCDLHKIHRDDLLEVLDMYPEFSDHFWSSLEITFNLRDTNMIPGSPGSTELEGGFSRQRKRKLSFRRRTDKDTEQPGEVSALGPGRAGAGPSSRGRPGGPWGESPSSGPSSPESSEDEGPGRSSSPLRLVPFSSPRPPGEPPGGEPLMEDCEKSSDTCNPLSGAFSGVSNIFSFWGDSRGRQYQELPRCPAPTPSLLNIPLSSPGRRPRGDVESRLDALQRQLNRLETRLSADMATVLQLLQRQMTLVPPAYSAVTTPGPGPTSTSPLLPVSPLPTLTLDSLSQVSQFMACEELPPGAPELPQEGPTRRLSLPGQLGALTSQPLHRHGSDPGS D03BD4F657641FBA kcnh2-kcnh2 2 HERG 4 unspecified role unspecified role unspecified role unspecified role purified purified range gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n_terminus sufficient to bind sufficient binding region sufficient to bind n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction Fig. 2A kcnh2-kcnh2-3 1 HERG 4 unspecified role unspecified role unspecified role unspecified role purified purified region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n_terminus sufficient to bind sufficient binding region sufficient to bind n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal certain certain sequence position certain ecolx Escherichia coli 4 unspecified role unspecified role unspecified role unspecified role purified purified n-terminus sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli 12 14 direct interaction direct interaction 1B kcnh2-1 2 HERG 4 unspecified role unspecified role unspecified role unspecified role purified purified n_terminus sufficient to bind sufficient binding region sufficient to bind n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal certain certain sequence position certain range gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecolx Escherichia coli direct interaction direct interaction Fig. 2A kcnh2-1 3 HERG 4 unspecified role unspecified role unspecified role unspecified role purified purified n_terminus sufficient to bind sufficient binding region sufficient to bind n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction Fig. 2B kcnh2-1 3 HERG 4 unspecified role unspecified role unspecified role unspecified role n_terminus sufficient to bind sufficient binding region sufficient to bind n-terminal n-terminal position amino-terminus n-terminus n-term n-term amino-terminus n-terminus n-terminal certain certain sequence position certain ecolx Escherichia coli direct interaction direct interaction Fig. 2B