bhf-ucl The 39-kDa receptor-associated protein regulates ligand binding by the very low density lipoprotein receptor. The 39-kDa receptor-associated protein regulates ligand binding by the very low density lipoprotein receptor. J. Biol. Chem. (0021-9258) 1994 imex curation Battey F.D., Gåfvels M.E., FitzGerald D.J., Argraves W.S., Chappell D.A., Strauss J.F., Strickland D.K. Cardiac - Interactions involving cardiac related proteins No email contact for author is mentioned in the paper. The expression system for human RAP was traced to the publication PMID 1374383 to be E Coli as per citation by author. The sources of the following proteins were determined as follows: RAP and VLDLR are both human as stated by author. 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra Battey F.D., Gåfvels M.E., FitzGerald D.J., Argraves W.S., Chappell D.A., Strauss J.F., Strickland D.K. Cardiac - Interactions involving cardiac related proteins No email contact for author is mentioned in the paper. Physical association instead of direct interaction because at point of first contact between the two proteins, RAP was purified while VLDLR was only partically purified on the RAP-sepharose column. In figure 3A, LRP was not annotated as a binding partner because it was identified solely based on molecular weight i.e. antibodies were not used. The expression system for human RAP was traced to the publication PMID 1374383 to be E Coli as per citation by author. Accepted 2013-DEC-04 by ORCHARD J. Biol. Chem. (0021-9258) 1994 imex curation The 39-kDa receptor-associated protein regulates ligand binding by the very low density lipoprotein receptor. The 39-kDa receptor-associated protein regulates ligand binding by the very low density lipoprotein receptor. J. Biol. Chem. (0021-9258) 1994 imex curation Battey F.D., Gåfvels M.E., FitzGerald D.J., Argraves W.S., Chappell D.A., Strauss J.F., Strickland D.K. Cardiac - Interactions involving cardiac related proteins No email contact for author is mentioned in the paper. The expression system for human RAP was traced to the publication PMID 1374383 to be E Coli as per citation by author. The sources of the following proteins were determined as follows: RAP and VLDLR are both human as stated by author. 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom polyclonal western polyclonal antibody western blot polyclonal western Battey F.D., Gåfvels M.E., FitzGerald D.J., Argraves W.S., Chappell D.A., Strauss J.F., Strickland D.K. Cardiac - Interactions involving cardiac related proteins No email contact for author is mentioned in the paper. I chose physical association instead of direct interaction because at point of first contact between the two proteins, RAP was purified while VLDLR was a lysate preparation. The expression system for human RAP was traced to the publication PMID 1374383 to be E Coli as per citation by author. Accepted 2013-DEC-04 by ORCHARD J. Biol. Chem. (0021-9258) 1994 imex curation The 39-kDa receptor-associated protein regulates ligand binding by the very low density lipoprotein receptor. The 39-kDa receptor-associated protein regulates ligand binding by the very low density lipoprotein receptor. J. Biol. Chem. (0021-9258) 1994 imex curation Battey F.D., Gåfvels M.E., FitzGerald D.J., Argraves W.S., Chappell D.A., Strauss J.F., Strickland D.K. Cardiac - Interactions involving cardiac related proteins No email contact for author is mentioned in the paper. The expression system for human RAP was traced to the publication PMID 1374383 to be E Coli as per citation by author. The sources of the following proteins were determined as follows: RAP and VLDLR are both human as stated by author. 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro far western blotting far western blotting Affinity blotting Affinity blotting polyclonal western polyclonal antibody western blot polyclonal western Battey F.D., Gåfvels M.E., FitzGerald D.J., Argraves W.S., Chappell D.A., Strauss J.F., Strickland D.K. Cardiac - Interactions involving cardiac related proteins No email contact for author is mentioned in the paper. No email contact for author is mentioned in the paper. No email contact for author is mentioned in the paper. Physical association instead of direct interaction is due to the VLDLR being a lysate prepration. The expression system for human RAP was traced to the publication PMID 1374383 to be E Coli as per citation by author. Accepted 2013-DEC-04 by ORCHARD J. Biol. Chem. (0021-9258) 1994 imex curation The 39-kDa receptor-associated protein regulates ligand binding by the very low density lipoprotein receptor. The 39-kDa receptor-associated protein regulates ligand binding by the very low density lipoprotein receptor. J. Biol. Chem. (0021-9258) 1994 imex curation Battey F.D., Gåfvels M.E., FitzGerald D.J., Argraves W.S., Chappell D.A., Strauss J.F., Strickland D.K. Cardiac - Interactions involving cardiac related proteins No email contact for author is mentioned in the paper. The expression system for human RAP was traced to the publication PMID 1374383 to be E Coli as per citation by author. The sources of the following proteins were determined as follows: RAP and VLDLR are both human as stated by author. 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Battey F.D., Gåfvels M.E., FitzGerald D.J., Argraves W.S., Chappell D.A., Strauss J.F., Strickland D.K. Cardiac - Interactions involving cardiac related proteins No email contact for author is mentioned in the paper. The expression system for human RAP was traced to the publication PMID 1374383 to be E Coli as per citation by author. Interactions with VLDL could not be captured as the origin of this protein was not given. Accepted 2013-DEC-04 by ORCHARD J. Biol. Chem. (0021-9258) 1994 imex curation vldlr_human Very low-density lipoprotein receptor VLDLR protein protein human Homo sapiens Human MGTSALWALWLLLALCWAPRESGATGTGRKAKCEPSQFQCTNGRCITLLWKCDGDEDCVDGSDEKNCVKKTCAESDFVCNNGQCVPSRWKCDGDPDCEDGSDESPEQCHMRTCRIHEISCGAHSTQCIPVSWRCDGENDCDSGEDEENCGNITCSPDEFTCSSGRCISRNFVCNGQDDCSDGSDELDCAPPTCGAHEFQCSTSSCIPISWVCDDDADCSDQSDESLEQCGRQPVIHTKCPASEIQCGSGECIHKKWRCDGDPDCKDGSDEVNCPSRTCRPDQFECEDGSCIHGSRQCNGIRDCVDGSDEVNCKNVNQCLGPGKFKCRSGECIDISKVCNQEQDCRDWSDEPLKECHINECLVNNGGCSHICKDLVIGYECDCAAGFELIDRKTCGDIDECQNPGICSQICINLKGGYKCECSRGYQMDLATGVCKAVGKEPSLIFTNRRDIRKIGLERKEYIQLVEQLRNTVALDADIAAQKLFWADLSQKAIFSASIDDKVGRHVKMIDNVYNPAAIAVDWVYKTIYWTDAASKTISVATLDGTKRKFLFNSDLREPASIAVDPLSGFVYWSDWGEPAKIEKAGMNGFDRRPLVTADIQWPNGITLDLIKSRLYWLDSKLHMLSSVDLNGQDRRIVLKSLEFLAHPLALTIFEDRVYWIDGENEAVYGANKFTGSELATLVNNLNDAQDIIVYHELVQPSGKNWCEEDMENGGCEYLCLPAPQINDHSPKYTCSCPSGYNVEENGRDCQSTATTVTYSETKDTNTTEISATSGLVPGGINVTTAVSEVSVPPKGTSAAWAILPLLLLVMAAVGGYLMWRNWQHKNMKSMNFDNPVYLKTTEEDLSIDIGRHSASVGHTYPAISVVSTDDDLA DIP protein P98155 original sequence version: 154 DIP protein P98155 original sequence version: 115 DIP protein P98155 original sequence version: 113 8BAC29438A78C2B8 amrp_human Alpha-2-macroglobulin receptor-associated protein Low density lipoprotein receptor-related protein-associated protein 1 LRPAP1 A2MRAP protein protein human Homo sapiens Human MAPRRVRSFLRGLPALLLLLLFLGPWPAASHGGKYSREKNQPKPSPKRESGEEFRMEKLNQLWEKAQRLHLPPVRLAELHADLKIQERDELAWKKLKLDGLDEDGEKEARLIRNLNVILAKYGLDGKKDARQVTSNSLSGTQEDGLDDPRLEKLWHKAKTSGKFSGEELDKLWREFLHHKEKVHEYNVLLETLSRTEEIHENVISPSDLSDIKGSVLHSRHTELKEKLRSINQGLDRLRRVSHQGYSTEAEFEEPRVIDLWDLAQSANLTDKELEAFREELKHFEAKIEKHNHYQKQLEIAHEKLRHAESVGDGERVSRSREKHALLEGRTKELGYTVKKHLQDLSGRISRARHNEL DIP protein P30533 original sequence version: 105 DIP protein P30533 original sequence version: 103 D56A7DA754125CFA lrpap1-vldlr-2 3 6 unspecified role unspecified role bait bait purified purified escherichia coli dh5[alpha] escherichia coli dh5[alpha] 5 unspecified role unspecified role prey prey cell lysate cell lysate crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association Figures 2 A, B, C and D lrpap1-vldlr-6 2 6 predetermined predetermined participant predetermined polyclonal western polyclonal antibody western blot polyclonal western unspecified role unspecified role bait bait purified purified escherichia coli dh5[alpha] escherichia coli dh5[alpha] 5 unspecified role unspecified role prey prey cell lysate cell lysate crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association Figure 3 C vldlr-lrpap1 4 5 unspecified role unspecified role bait bait partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. 6 unspecified role unspecified role prey prey purified purified escherichia coli dh5[alpha] escherichia coli dh5[alpha] direct interaction direct interaction Figure 4 A lrpap1-vldlr-5 1 6 unspecified role unspecified role bait bait purified purified 125i radiolabel 125i radiolabel 125i radiolabel I125 125I 125I I125 undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined escherichia coli dh5[alpha] escherichia coli dh5[alpha] 5 polyclonal western polyclonal antibody western blot polyclonal western weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra unspecified role unspecified role prey prey partially purified partially purified crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. physical association physical association Figure 3 A