IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ An extracellular adhesion molecule complex patterns dendritic branching and morphogenesis. An extracellular adhesion molecule complex patterns dendritic branching and morphogenesis. Cell (0092-8674) 2013 imex curation Dong X., Liu O.W., Howell A.S., Shen K. kangshen@stanford.edu 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro aggregation aggregation assay aggregation tag fluorescence tag visualisation by fluorescence tag fluorescence Dong X., Liu O.W., Howell A.S., Shen K. I'll update the definition of 'aggregation' to include this use case as it looked stronger evidence than a standard co-localisation. kangshen@stanford.edu Accepted 2013-DEC-11 by PPORRAS Cell (0092-8674) 2013 Only protein-protein interactions imex curation An extracellular adhesion molecule complex patterns dendritic branching and morphogenesis. An extracellular adhesion molecule complex patterns dendritic branching and morphogenesis. Cell (0092-8674) 2013 imex curation Dong X., Liu O.W., Howell A.S., Shen K. kangshen@stanford.edu 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Dong X., Liu O.W., Howell A.S., Shen K. DMA-1 and MNR-1 could only be coprecipitated when lysates of SAX-7::HA, MNR-1::FLAG cotransfected cells were mixed with DMA-1::myc lysate and anti-HA beads kangshen@stanford.edu Accepted 2013-DEC-11 by PPORRAS Cell (0092-8674) 2013 Only protein-protein interactions imex curation q22075_caeel dma-1 CELE_T01G9.3 T01G9.3 protein protein caeel Caenorhabditis elegans MISYILLLFLSFLVLCSIALPSSCPNLCECDQNDSSWSVYCRKAIINDTIYAEILNQLPLTLRSLHIQPPSNRIGSNKLRWNDNINRFAQLRVLRLINCQIPAMSRSIRLPSLEVLDLHSNNIEHATMSNFGGMPKLRVLDLSSNHLNILPTGVFTYLRALRSLSLSNNTISDLSTNLLRGLNSLRVLRLDRNPIPIEHINELFTDVSQLDELYLNHCNLSSIYSLALDRIPQLRQLGIGGNNLKMVPTKELRSLPQLSVLDLSHNSIQEITACAFCNTNISKLDLSHNLLGISKDSPFNEDAFRTMPLRHLDLSFNHMNDFDSKWLGWAQEELTSIALSGNFLKNFEESWTYTLKSLIHLELAYNHIKFIPVQLPSRYYHLISLNISGNELTYLPDNINTLLPNVKTFDITANRFHTFSHTDLAFLNNVEQVYVDGNPWDCSCAIQGLQVHMRDRYAMRHILNYDNVRCATPSLVEGHSVLAITDVNDCAVLFGARYGLTQTSEMLILLAGVLLFAALLLMILGCIYFLRERQYKGSYVTREHSRTPLTMANTHSCSSSTNDTHGPLSPPFDPFLVSTETFKATPPLIPPAPPKPGSSYFGI 268AF1D42127DE0B q53u87_caeel sax-7 C18F3.2 CELE_C18F3.2 protein protein caeel Caenorhabditis elegans MRSFIFLLLLTGHISSGSIDWSAIETPPQFVHDKNADTVYFKVEKSGMSDESAKNTPGNLLEQTIHCYANGNPRPSYRWKKDGKTFLPSMFPEKVVQKPGEGSLVFSRLDESDAGLYQCEAENSNGTAVDRPVRVQETWIRHFKTAEPEVVVVEVGDPYQRNCSPPASNPYARVYWILMGKEPGHFETISSSHISSNEQGTLFFHYVNETDFKSDRYYTCTAENIELKDYKFGNQFSLQITNNKRRSLQQMPPTEQYVNQSSPIALQGNQHKLHCFFSGYPAPKPRWFHNGREISEDSDAAGFRFESYGKTLVFNVTQDKAGKYDCRFATQQDIDRTFNVVVEAAPYWPLGPPPNTNTSEGEKVIFDCTTYGKPTPKVTFYKNGVEIESQKDDRYLIEGTRLTIFDVKKGTYGKGDNAVYQCKSENKHGWLWTNFYLNLLAFKPQLLIDPGKEEVEAVAGKKVTLECKFFASPNAAVKWEAPMISGSKGNQIPADAYGVGKLVFSEVTAAEEGEYECIGTNKYGQATGLITLKVRKPTIVQPFPRVEEVRMAGEEMRLACDATADNQLEVKYEWLVDGKSLPEDRISSGHYKIDDDHSLVISNPTQDDTAKYKCVVSTKLDQVEKEIKIQFKDVPVAVHSAWVQTCDKNSLTAFIKFEHYESIHTIAPIKEFWVQYQIDSETEGSQWRTHPVPSAAHPNDKIDNNLRHTTGDATVSLQPFGKYVFRVIARNSVGDSAARLAKDQCETPAKQPDKNPDEVAAKGTSPENIIVQWKPMSREEWNGADFHYVVKYRPKDEDQRVGDWKEVAVEDPFADRVTVNLDDEKDVKPFQPYEVQVQAVNSEGRTNVVPETVEGRTGEGVPSSIPSGLRVLEKSGTTVTLAWNGVDPQTANGNFTGYKITYWVDEADQDSDDSSEDDEDDEKRKFRWKRSIRVKRQSGIRKTVVFGPSATQGTLTDLKPATLNHAYIQVTNGAHEGSASDTIDFQTDEGVPSPVRSLRAYPMNSKVGGEKGVVVLVWKKPRQTNGKLARYEVEYCKTQNGKLVEKSCPRKQIDADSKEIRITGLENETPYRFILRAHTSAGEGDPNSSDATTLPETTAAGVDPGVPSLVENAIGDKYFNVSFRPAKYDDESRAPVGNTYEVQYKPQDADEWETVKPADDGLTVHVDGLSPGTKYDVRVAALQVDPEGGETTKTLSGISKITTTGTSSRERNVYLLILLLLILLLLLIICCICCVVCRQRGQNYPVSQREREQGREPILGKPDYKTDDDEKRSLTGSKAESETDSMAQYGDTDPGVFTEDGSFIAVSGQYVPQKSLMPAERPEKGSTSTFV A8AF1AC13582451A o45879_caeel mnr-1 CELE_W01F3.1 W01F3.1 protein protein caeel Caenorhabditis elegans MMISILLVFAVSTAYCRDYGREMRATRMSVVDFWKNETKGDGLNVRVAHGVNSWPDLVDQLHEPFLNKSMMIEGDVFMQAHRRPRHRAVPVMKADTKLADRITFKEWLREVATMNKAIKINFRSNEVVRPVLQDLYASQADPTSPVLQYPVILHANVFRSPRSVETEVDPSTFVEKAKDLFPDATLSLGWTKQSNFSHLHPKFKKLSWRQLFHILEYISRLDQPVMLSVRLSVAAHSKEQLLWLLGMDQSISLLLWSDAEDHVTNWTPIVELRRSTTKNRILYDLDPKHRKFLQTDSNEPIATPPTFSLQDWQAVEFSSSGSQLSTVVRSEKGPAFLGEPTALLLSKIPPPNFPSEQKISGKVHFLPKKGMENVDLDENSGVSIYLMDKVQDIDSPKIKNSLEVFIGFDGKIKITNGQLKNLPYYETKSVGQLPDSDCYGFEVTDMGWRVKLDAWTAECGNQKLRKRRTVSIELDTPFQKNRHLRNVVVSKKGDSAVDFLLEELQHKNAYNYRNPWITVVSTILLMIYLL E86C3006B8BC7D90 sax_7-dma_1 2 5 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. 3 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined extracellular region sufficient to bind sufficient binding region sufficient to bind range range range range drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. 4 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. association association Fig. 7E dma_1-o45879 1 5 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. 3 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. 4 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind range range certain certain sequence position certain region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. physical association physical association Fig. 7C, Supp. Fig. 6D