IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Spikar, a novel drebrin-binding protein, regulates the formation and stabilization of dendritic spines. Spikar, a novel drebrin-binding protein, regulates the formation and stabilization of dendritic spines. J. Neurochem. (0022-3042) 2013 imex curation Yamazaki H., Kojima N., Kato K., Hirose E., Iwasaki T., Mizui T., Takahashi H., Hanamura K., Roppongi R.T., Koibuchi N., Sekino Y., Mori N., Shirao T. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease tshirao@med.gunma-u.ac.jp 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions rat-primary_hippocampal_neuron Rat primary hippocampal neuron primary hippocampal neurons Rat primary hippocampal neurons fluorescence imaging fluorescence microscopy fluorescence imaging immunocytochemistry immunocytochemistry Yamazaki H., Kojima N., Kato K., Hirose E., Iwasaki T., Mizui T., Takahashi H., Hanamura K., Roppongi R.T., Koibuchi N., Sekino Y., Mori N., Shirao T. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. tshirao@med.gunma-u.ac.jp Accepted 2014-JAN-06 by ORCHARD rat brain cDNA library J. Neurochem. (0022-3042) 2013 Only protein-protein interactions imex curation Spikar, a novel drebrin-binding protein, regulates the formation and stabilization of dendritic spines. Spikar, a novel drebrin-binding protein, regulates the formation and stabilization of dendritic spines. J. Neurochem. (0022-3042) 2013 imex curation Yamazaki H., Kojima N., Kato K., Hirose E., Iwasaki T., Mizui T., Takahashi H., Hanamura K., Roppongi R.T., Koibuchi N., Sekino Y., Mori N., Shirao T. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease tshirao@med.gunma-u.ac.jp 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. fluorescence imaging fluorescence microscopy fluorescence imaging tag fluorescence tag visualisation by fluorescence tag fluorescence Yamazaki H., Kojima N., Kato K., Hirose E., Iwasaki T., Mizui T., Takahashi H., Hanamura K., Roppongi R.T., Koibuchi N., Sekino Y., Mori N., Shirao T. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. tshirao@med.gunma-u.ac.jp Accepted 2014-JAN-06 by ORCHARD rat brain cDNA library J. Neurochem. (0022-3042) 2013 Only protein-protein interactions imex curation Spikar, a novel drebrin-binding protein, regulates the formation and stabilization of dendritic spines. Spikar, a novel drebrin-binding protein, regulates the formation and stabilization of dendritic spines. J. Neurochem. (0022-3042) 2013 imex curation Yamazaki H., Kojima N., Kato K., Hirose E., Iwasaki T., Mizui T., Takahashi H., Hanamura K., Roppongi R.T., Koibuchi N., Sekino Y., Mori N., Shirao T. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease tshirao@med.gunma-u.ac.jp 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Yamazaki H., Kojima N., Kato K., Hirose E., Iwasaki T., Mizui T., Takahashi H., Hanamura K., Roppongi R.T., Koibuchi N., Sekino Y., Mori N., Shirao T. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. tshirao@med.gunma-u.ac.jp Accepted 2014-JAN-06 by ORCHARD rat brain cDNA library J. Neurochem. (0022-3042) 2013 Only protein-protein interactions imex curation Spikar, a novel drebrin-binding protein, regulates the formation and stabilization of dendritic spines. Spikar, a novel drebrin-binding protein, regulates the formation and stabilization of dendritic spines. J. Neurochem. (0022-3042) 2013 imex curation Yamazaki H., Kojima N., Kato K., Hirose E., Iwasaki T., Mizui T., Takahashi H., Hanamura K., Roppongi R.T., Koibuchi N., Sekino Y., Mori N., Shirao T. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease tshirao@med.gunma-u.ac.jp 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Yamazaki H., Kojima N., Kato K., Hirose E., Iwasaki T., Mizui T., Takahashi H., Hanamura K., Roppongi R.T., Koibuchi N., Sekino Y., Mori N., Shirao T. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Spikar has been entered as 'expressed in' as Rat hippocampus, however no cell type/line or origin is given for this molecule. tshirao@med.gunma-u.ac.jp Accepted 2014-JAN-06 by ORCHARD J. Neurochem. (0022-3042) 2013 Only protein-protein interactions imex curation Spikar, a novel drebrin-binding protein, regulates the formation and stabilization of dendritic spines. Spikar, a novel drebrin-binding protein, regulates the formation and stabilization of dendritic spines. J. Neurochem. (0022-3042) 2013 imex curation Yamazaki H., Kojima N., Kato K., Hirose E., Iwasaki T., Mizui T., Takahashi H., Hanamura K., Roppongi R.T., Koibuchi N., Sekino Y., Mori N., Shirao T. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease tshirao@med.gunma-u.ac.jp 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Yamazaki H., Kojima N., Kato K., Hirose E., Iwasaki T., Mizui T., Takahashi H., Hanamura K., Roppongi R.T., Koibuchi N., Sekino Y., Mori N., Shirao T. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Supplementary Figure 5 the diagrams have been labelled "a" and "b" yet the legend refers to "e" and "f". tshirao@med.gunma-u.ac.jp Accepted 2014-JAN-06 by ORCHARD J. Neurochem. (0022-3042) 2013 Only protein-protein interactions imex curation dreb_rat Drebrin Dbn1 Developmentally-regulated brain protein protein protein rat Rattus norvegicus (Rat) Rat MAGVSFSGHRLELLAAYEEVIREESAADWALYTYEDGSDDLKLAASGEGGLQELSGHFENQKVMYGFCSVKDSQAALPKYVLINWVGEDVPDARKCACASHVAKVAEFFQGVDVIVNASSVEDIDAGAIGQRLSNGLARLSSPVLHRLRLREDENAEPVGTTYQKTDAAVEMKRINREQFWEQAKKEEELRKEEERKKALDARLRFEQERMEQERQEQEERERRYREREQQIEEHRRKQQSLEAEEAKRRLKDQSIFGDQRDEEEESQMKKSESEVEEAAAIIAQRPDNPREFFRQQERVASASGGSCDAPSPFNHRPGRPYCPFIKASDSGPSSSSSSSSSPPRTPFPYITCHRTPNLSSSLPCSHLDSHRRMAPTPIPTRSPSDSSTASTPITEQIERALDEVTSSQPPPPPPPPPPAQEAQESAPRLDGEEVCKEAKVAAAPQVWAGCAEEPPRAQEPPLLQSSPTEDLMCTESPEQAVLAASPEPDASVTSVADAHAADTIETTTATTATTIADNVTPAAASLIDLWPGNGEEASTPQAEPRVPTPPSGAEASLAEVPLLNEAAQEPLPPVGEGCANLLNFDELPEPPATFCDPEEEAEGEPLAASQVLTMPSALEEVDQVLEQELEPEPHLLTNGETTQKEGTQQASEGYFSQSQEEEFAQSEEPCAKAPPPVFYNKPPEIDITCWDADPVPEEEEGFEGGD B5279BF6EB7B80AA a8c4g9_rat Zmynd8 protein protein rat Rattus norvegicus (Rat) Rat MDISTRSKDPGSTERTAQKRKVPSPPHSSNGHSPQDSSTSPIKKKKKPGLLNSSSKDQSELRHGPFYYMKQPLTTDPVDVVPQDGRNDFYCWVCHREGQVLCCELCPRVYHAKCLRLTSEPEGDWFCPECEKITVAECIETQSKAMTMLTIEQLSYLLKFAIQKMKQPGTDAFQKPVPLEQHPDYAEYIFHPMDLCTLEKNAKKKMYGCTEAFLADAKWILHNCIIYNGGNHKLTQIAKVVIKICEHEMNEIEVCPECYLAACQKRDNWFCEPCSNPHPLVWAKLKGFPFWPAKALRDKDGQVDARFFGQHDRAWVPVNNCYLMSKEIPFSVKKTKSIFNSAMQEMEVYVENIRKKFGVFNYSPFRTPYTPNSQYQMLLDPSNPSAGTAKTDKQEKVKLNFDMTASPKIILSKPLLSGGAGRRISLSDMPRSPTSTNSSVHTGSDVEQDPEKKAPSSHFSASEESMDFLDKSTASPASTKTGQAGSLSGSPKPFSPQAPTPIMTKPDKTSTATTGSILNLNLDRSKAEMDLKELSESVQQQSAPVPLISPKRQIRSRFQLNLDKTIESCKAQLGINEISEDVYTAVEHSDSEDSEKSESSDSEYVSDEEQKPKNEPEDPEDKEGSRVDKEAPAIKRKPKPSNQVEVKEEVKSNSPVGEKVDPAPAKDKASPEPEKDFAEKAKPSPHPTKDKLKGKDETDSPTVHLGLDSDSESELVIDLGEDPSGREGRKNKKEPKVPSPKQDAIGKPPPPTPAGNQSPPETPVLTRSATQAPAAGVTVAATTSTMSTVTVTAPATAAVTGSPVKKQRPLLPKETVPAVQRVVWNASSKFQTSSQKWHMQKIQRQQQQQQQQQQQQQSQQQQPQSSQGTRYQTRQAVKAVQQKEVTQSPSTSTITLVTSTQPAALVSSSGSASTLASVINADLPIATASADVAADIAKYTSKMMDAIKGTMTEIYNDLSKNTTGSTIAEIRRLRIEIEKLQWLHQQELAEMKHNLELTMAEMRQSLEQERDRLIAEVKKQLELEKQQAVDETKKKQWCANCKKEAIFYCCWNTSYCDYPCQQAHWPEHMKSCTQSATAPQQEADAEANQETGNKTSQGSSSNTQSAPSEPASTPKEKETSSSSSNSSSTEKSKDSNSTLDLSGSRETPSSILLGSNQGSDHSRSSKAGLWSSSEEKRGSSRSEHSAGTSTKNLMPKESRESRLDTFWD B25CB7EF2D41B352 dbn1-zmynd8 3 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association no figure dbn1-zmynd8-1 4 7 unspecified role unspecified role prey prey n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role bait bait n_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) physical association physical association 1D Spikar - entered as 'expressed in' as Rat hippo - the most likely cell type - Spikar transfected 293 cells are used later on, however. dbn1-zmynd8-2 4 7 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role bait bait n_terminus gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 20 23 physical association physical association 1E Spikar - entered as 'expressed in' as Rat hippo - the most likely cell type - Spikar transfected 293 cells are used later on, however. dbn1-zmynd8-4 1 7 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization 2D dbn1-zmynd8-5 1 6 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization 2E dbn1-zmynd8-6 1 7 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role neutral component neutral component colocalization colocalization 6D/E dbn1-zmynd8-6 2 6 unspecified role unspecified role neutral component neutral component region rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role neutral component neutral component n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization 6A/B/C dbn1-zmynd8-7 5 7 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 6 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Supp 5 A (E) dbn1-zmynd8-8 5 6 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 7 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 49 47 physical association physical association Supp 5 B (F)