IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Molecular Architecture of the ATP-Dependent Chromatin-Remodeling Complex SWR1. Molecular Architecture of the ATP-Dependent Chromatin-Remodeling Complex SWR1. Cell (0092-8674) 2013 imex curation Nguyen V.Q., Ranjan A., Stengel F., Wei D., Aebersold R., Wu C., Leschziner A.E. I know we don't usually accept staining but this complex is subsequently looked at by EM so it seems silly not to take that data and not this. Affinity-purified sample was run through a combined glycerol and crosslinker gradient. Small aliquots from all the gradient fractions were treated to reverse the formaldehyde crosslinks and then analyzed by SDS-PAGE aleschziner@mcb.harvard.edu, 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast density sedimentation cosedimentation through density gradient density sedimentation weight by sybr molecular weight estimation by sybr staining safe DNA gel stain safe DNA gel stain weight by sybr Nguyen V.Q., Ranjan A., Stengel F., Wei D., Aebersold R., Wu C., Leschziner A.E. I know we don't usually accept staining but this complex is subsequently looked at by EM so it seems silly not to take that data and not this. Affinity-purified sample was run through a combined glycerol and crosslinker gradient. Small aliquots from all the gradient fractions were treated to reverse the formaldehyde crosslinks and then analyzed by SDS-PAGE aleschziner@mcb.harvard.edu, Accepted 2013-NOV-08 by MGT 22-Nov-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation Molecular Architecture of the ATP-Dependent Chromatin-Remodeling Complex SWR1. Molecular Architecture of the ATP-Dependent Chromatin-Remodeling Complex SWR1. Cell (0092-8674) 2013 imex curation Nguyen V.Q., Ranjan A., Stengel F., Wei D., Aebersold R., Wu C., Leschziner A.E. I know we don't usually accept staining but this complex is subsequently looked at by EM so it seems silly not to take that data and not this. Affinity-purified sample was run through a combined glycerol and crosslinker gradient. Small aliquots from all the gradient fractions were treated to reverse the formaldehyde crosslinks and then analyzed by SDS-PAGE aleschziner@mcb.harvard.edu, 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Nguyen V.Q., Ranjan A., Stengel F., Wei D., Aebersold R., Wu C., Leschziner A.E. I know we don't usually accept staining but this complex is subsequently looked at by EM so it seems silly not to take that data and not this. Affinity-purified sample was run through a combined glycerol and crosslinker gradient. Small aliquots from all the gradient fractions were treated to reverse the formaldehyde crosslinks and then analyzed by SDS-PAGE aleschziner@mcb.harvard.edu, Accepted 2013-NOV-08 by MGT 22-Nov-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation Molecular Architecture of the ATP-Dependent Chromatin-Remodeling Complex SWR1. Molecular Architecture of the ATP-Dependent Chromatin-Remodeling Complex SWR1. Cell (0092-8674) 2013 imex curation Nguyen V.Q., Ranjan A., Stengel F., Wei D., Aebersold R., Wu C., Leschziner A.E. I know we don't usually accept staining but this complex is subsequently looked at by EM so it seems silly not to take that data and not this. Affinity-purified sample was run through a combined glycerol and crosslinker gradient. Small aliquots from all the gradient fractions were treated to reverse the formaldehyde crosslinks and then analyzed by SDS-PAGE aleschziner@mcb.harvard.edu, 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast crosslink cross-linking study crosslink sequence tag sequence tag identification sequence tag Nguyen V.Q., Ranjan A., Stengel F., Wei D., Aebersold R., Wu C., Leschziner A.E. I know we don't usually accept staining but this complex is subsequently looked at by EM so it seems silly not to take that data and not this. Affinity-purified sample was run through a combined glycerol and crosslinker gradient. Small aliquots from all the gradient fractions were treated to reverse the formaldehyde crosslinks and then analyzed by SDS-PAGE aleschziner@mcb.harvard.edu, Accepted 2013-NOV-08 by MGT 22-Nov-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation Molecular Architecture of the ATP-Dependent Chromatin-Remodeling Complex SWR1. Molecular Architecture of the ATP-Dependent Chromatin-Remodeling Complex SWR1. Cell (0092-8674) 2013 imex curation Nguyen V.Q., Ranjan A., Stengel F., Wei D., Aebersold R., Wu C., Leschziner A.E. I know we don't usually accept staining but this complex is subsequently looked at by EM so it seems silly not to take that data and not this. Affinity-purified sample was run through a combined glycerol and crosslinker gradient. Small aliquots from all the gradient fractions were treated to reverse the formaldehyde crosslinks and then analyzed by SDS-PAGE aleschziner@mcb.harvard.edu, 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy predetermined predetermined participant predetermined Nguyen V.Q., Ranjan A., Stengel F., Wei D., Aebersold R., Wu C., Leschziner A.E. I know we don't usually accept staining but this complex is subsequently looked at by EM so it seems silly not to take that data and not this. Affinity-purified sample was run through a combined glycerol and crosslinker gradient. Small aliquots from all the gradient fractions were treated to reverse the formaldehyde crosslinks and then analyzed by SDS-PAGE EMD-5638 (SWR1 + nucleosome) could not be captured as a list or participants could not be traced. aleschziner@mcb.harvard.edu, Accepted 2013-NOV-08 by MGT 22-Nov-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation Molecular Architecture of the ATP-Dependent Chromatin-Remodeling Complex SWR1. Molecular Architecture of the ATP-Dependent Chromatin-Remodeling Complex SWR1. Cell (0092-8674) 2013 imex curation Nguyen V.Q., Ranjan A., Stengel F., Wei D., Aebersold R., Wu C., Leschziner A.E. I know we don't usually accept staining but this complex is subsequently looked at by EM so it seems silly not to take that data and not this. Affinity-purified sample was run through a combined glycerol and crosslinker gradient. Small aliquots from all the gradient fractions were treated to reverse the formaldehyde crosslinks and then analyzed by SDS-PAGE aleschziner@mcb.harvard.edu, 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast density sedimentation cosedimentation through density gradient density sedimentation weight by comassie molecular weight estimation by coomasie staining weight by comassie Nguyen V.Q., Ranjan A., Stengel F., Wei D., Aebersold R., Wu C., Leschziner A.E. I know we don't usually accept staining but this complex is subsequently looked at by EM so it seems silly not to take that data and not this. Affinity-purified sample was run through a combined glycerol and crosslinker gradient. Small aliquots from all the gradient fractions were treated to reverse the formaldehyde crosslinks and then analyzed by SDS-PAGE aleschziner@mcb.harvard.edu, Accepted 2013-NOV-08 by MGT 22-Nov-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation Molecular Architecture of the ATP-Dependent Chromatin-Remodeling Complex SWR1. Molecular Architecture of the ATP-Dependent Chromatin-Remodeling Complex SWR1. Cell (0092-8674) 2013 imex curation Nguyen V.Q., Ranjan A., Stengel F., Wei D., Aebersold R., Wu C., Leschziner A.E. I know we don't usually accept staining but this complex is subsequently looked at by EM so it seems silly not to take that data and not this. Affinity-purified sample was run through a combined glycerol and crosslinker gradient. Small aliquots from all the gradient fractions were treated to reverse the formaldehyde crosslinks and then analyzed by SDS-PAGE aleschziner@mcb.harvard.edu, 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast density sedimentation cosedimentation through density gradient density sedimentation weight silver stain molecular weight estimation by silver staining weight silver stain Nguyen V.Q., Ranjan A., Stengel F., Wei D., Aebersold R., Wu C., Leschziner A.E. I know we don't usually accept staining but this complex is subsequently looked at by EM so it seems silly not to take that data and not this. Affinity-purified sample was run through a combined glycerol and crosslinker gradient. Small aliquots from all the gradient fractions were treated to reverse the formaldehyde crosslinks and then analyzed by SDS-PAGE aleschziner@mcb.harvard.edu, Accepted 2013-NOV-08 by MGT 22-Nov-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation Molecular Architecture of the ATP-Dependent Chromatin-Remodeling Complex SWR1. Molecular Architecture of the ATP-Dependent Chromatin-Remodeling Complex SWR1. Cell (0092-8674) 2013 imex curation Nguyen V.Q., Ranjan A., Stengel F., Wei D., Aebersold R., Wu C., Leschziner A.E. I know we don't usually accept staining but this complex is subsequently looked at by EM so it seems silly not to take that data and not this. Affinity-purified sample was run through a combined glycerol and crosslinker gradient. Small aliquots from all the gradient fractions were treated to reverse the formaldehyde crosslinks and then analyzed by SDS-PAGE aleschziner@mcb.harvard.edu, 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast crosslink cross-linking study crosslink weight by sybr molecular weight estimation by sybr staining safe DNA gel stain safe DNA gel stain weight by sybr Nguyen V.Q., Ranjan A., Stengel F., Wei D., Aebersold R., Wu C., Leschziner A.E. I know we don't usually accept staining but this complex is subsequently looked at by EM so it seems silly not to take that data and not this. Affinity-purified sample was run through a combined glycerol and crosslinker gradient. Small aliquots from all the gradient fractions were treated to reverse the formaldehyde crosslinks and then analyzed by SDS-PAGE aleschziner@mcb.harvard.edu, Accepted 2013-NOV-08 by MGT 22-Nov-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation taf14_yeast Transcription initiation factor TFIID subunit 14 ANC1 CST10 YPL129W TAF14 SWP29 TAF30 TFG3 TBP-associated factor 14 TBP-associated factor 30 kDa Transcription initiation factor TFIIF 30 kDa subunit Transcription factor G 30 kDa subunit SWI/SNF chromatin-remodeling complex subunit TAF14 SWI/SNF complex subunit TAF14 SWI/SNF complex 29 kDa subunit Actin non-complementing mutant 1 Chromosome stability protein 10 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVATVKRTIRIKTQQHILPEVPPVENFPVRQWSIEIVLLDDEGKEIPATIFDKVIYHLHPTFANPNRTFTDPPFRIEEQGWGGFPLDISVFLLEKAGERKIPHDLNFLQESYEVEHVIQIPLNKPLLTEELAKSGSTEETTANTGTIGKRRTTTNTTAEPKAKRAKTGSASTVKGSVDLEKLAFGLTKLNEDDLVGVVQMVTDNKTPEMNVTNNVEEGEFIIDLYSLPEGLLKSLWDYVKKNTE 60EA5EC2474B1B9C 60EA5EC2474B1B9C arp5_yeast Actin-related protein 5 ARP5 YNL059C N2430 YNL2430C Actin-like protein ARP5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSRDASLTPLKAVVIDDPPLRQTPEPFDEQSAYNPQSPIAIDFGSSKLRAGFVNHATPTHIFPNALTKFRDRKLNKNFTFVGNDTLLDQAVRSQSRSPFDGPFVTNWNLTEEILDYTFHHLGVVPDNGIPNPILLTERLATVQSQRTNWYQILFETYNVPGVTFGIDSLFSFYNYNPSGNKTGLVISCGHEDTNVIPVVDGAGILTDAKRINWGGHQAVDYLNDLMALKYPYFPTKMSYLQYETMYKDYCYVSRNYDEDIEKILTLENLDTNDVVVEAPFTEVLQPQKTEEELRIQAEKRKETGKRLQEQARLKRMEKLVQKQEEFEYFSKVRDQLIDEPKKKVLSVLQNAGFDDERDFKKYLHSLEQSLKKAQMVEAEDDSHLDEMNEDKTAQKFDLLDIADEDLNEDQIKEKRKQRFLKASQDARQKAKEEKERVAKEEEEKKLKEQQWRETDLNGWIKDKRLKLNKLIKRRKEKLKLRDEMKDRKSQVSQNRMKNLASLAEDNVKQGAKRNRHQATIDNDPNDTFGANDEDWLIYTDITQNPEAFEEALEYEYKDIVELERLLLEHDPNFTEEDTLEAQYDWRNSILHLFLRGPRPHDSENIHEQHQMHLNVERIRVPEVIFQPTMGGQDQAGICELSETILLKKFGSQPGKLSQTSIDMVNNVLITGGNAKVPGLKERIVKEFTGFLPTGTNITVNMSSDPSLDAWKGMAALARNEEQYRKTVISKKEYEEYGPEYIKEHKLGNTKYFED D8748B9096B32B53 swc7_yeast SWR1-complex protein 7 SWC7 AWS1 YLR385C protein protein yeast Saccharomyces cerevisiae Baker's yeast MDCPSNVVLLLLQLVLQRQQTLAHRDKSVDLQTLLKDPVIDNDVLVEFKTHKLVQLYGPQYCRDISLRGLKTMVTDIFANGIPKNAQSSGNDQPVTVVDLANYYYMQRINELQNTELPQLKEALLTRLEHMI 92CCE2FF3DCE72F1 92CCE2FF3DCE72F1 arp8_yeast Actin-like protein ARP8 ARP8 YOR3348C YOR141C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQEEAESSIIYEEPIDIPLEDDDDEDELEEENSVPLSSQADQENAENESDDSVDNVVGSETPRSVTGLSVDPRDVADEEDEDEEGEDEDEDEDDNDVDNEDENDNDNANENENELGSSRDKRAPPAVQTSKRYKKYPKLDPAKAPPGKKVPLHLLEKRRLGRIKAAEEFAKTLKKIGIEKVETTTLPATGLFQPLMLINQKNYSSDYLKKDDQIFALRDRKFLRNNNTSQISSTNTPDVIDLKSLPHSEASAAPLNDEIDLNDPTATIVIHPGSNSIKIGFPKDDHPVVVPNCVAVPKKWLDLENSEHVENVCLQREQSEEFNNIKSEMEKNFRERMRYYKRKVPGNAHEQVVSFNENSKPEIISEKNDPSPIEWIFDDSKLYYGSDALRCVDEKFVIRKPFRGGSFNVKSPYYKSLAELISDVTKLLEHALNSETLNVKPTKFNQYKVVLVIPDIFKKSHVETFIRVLLTELQFQAVAIIQESLATCYGAGISTSTCVVNIGAAETRIACVDEGTVLEHSAITLDYGGDDITRLFALFLLQSDFPLQDWKIDSKHGWLLAERLKKNFTTFQDADVAVQLYNFMNRSPNQPTEKYEFKLFDEVMLAPLALFFPQIFKLIRTSSHKNSSLEFQLPESRDLFTNELNDWNSLSQFESKEGNLYCDLNDDLKILNRILDAHNIIDQLQDKPENYGNTLKENFAPLEKAIVQSIANASITADVTRMNSFYSNILIVGGSSKIPALDFILTDRINIWRPSLLSSASFPQFYKKLTKEIKDLEGHYVNAPDKTEDENKQILQAQIKEKIVEELEEQHQNIEHQNGNEHIFPVSIIPPPRDMNPALIIWKGASVLAQIKLVEELFITNSDWDVHGSRILQYKCIFTY 8174851B6B077A19 8174851B6B077A19 ruvb2_yeast RuvB-like protein 2 TIP49-homology protein 2 TIP49b homolog RVB2 TIH2 TIP49B YPL235W P1060 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSIQTSDPNETSDLKSLSLIAAHSHITGLGLDENLQPRPTSEGMVGQLQARRAAGVILKMVQNGTIAGRAVLVAGPPSTGKTALAMGVSQSLGKDVPFTAIAGSEIFSLELSKTEALTQAFRKSIGIKIKEETELIEGEVVEIQIDRSITGGHKQGKLTIKTTDMETIYELGNKMIDGLTKEKVLAGDVISIDKASGKITKLGRSFARSRDYDAMGADTRFVQCPEGELQKRKTVVHTVSLHEIDVINSRTQGFLALFTGDTGEIRSEVRDQINTKVAEWKEEGKAEIVPGVLFIDEVHMLDIECFSFINRALEDEFAPIVMMATNRGVSKTRGTNYKSPHGLPLDLLDRSIIITTKSYNEQEIKTILSIRAQEEEVELSSDALDLLTKTGVETSLRYSSNLISVAQQIAMKRKNNTVEVEDVKRAYLLFLDSARSVKYVQENESQYIDDQGNVQISIAKSADPDAMDTTE 0D8EFBA7EC711AB8 0D8EFBA7EC711AB8 af9_yeast Protein AF-9 homolog N1966 YNL107W YAF9 protein protein yeast Saccharomyces cerevisiae Baker's yeast MAPTISKRIKTLSVSRPIIYGNTAKKMGSVKPPNAPAEHTHLWTIFVRGPQNEDISYFIKKVVFKLHDTYPNPVRSIEAPPFELTETGWGEFDINIKVYFVEEANEKVLNFYHRLRLHPYANPVPNSDNGNEQNTTDHNSKDAEVSSVYFDEIVFNEPNEEFFKILMSRPGNLLPSNKTDDCVYSKQLEQEEIDRIEIGIEKVDKEIDELKQKLENLVKQEAINGS 1FE08F72942061D1 1FE08F72942061D1 swr1_yeast Helicase SWR1 Swi2/Snf2-related 1 SWR1 YDR334W D9651.6 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTTSRKSHAKDKKAGGEQDLADLKFRYDLLTNELFHLREFVSLVDYDPTHFNDSESFQKFLRETHLSLEERGEKFTDDVAKKGTNGDLTRRRRNLRTSTVVSSETTNEKKGDIELKLESIAPLVRNKCEELKYKLSDHSNRKSIVPQKRPIQHLKKREAAKSLKFKSERKENPLPLHEHIAEERYDHIAKVEEPSEAFTIKCPSDDSSFENTSEHYSDNFYFTTSSEEEDIKKKRGRKKKKPRIKLVVHPPKQTITNPLHVVKPGYESLHEYIASFKSLEDDLTLEEYNKYIDEQRRLLSRLKKGIENGALKYDKETDSLQPITSKEIKTIITYKPDPISYFYKQQDLQIHTDHLINQGIHMSKLFRSSTKARIARAKKVSQMIEQHFKHVAGAEERKAKEEERHKKSLARFAVQAVKKRWNMAEKAYRILRKDEEEQLKRIEGKQHLSKMLEKSTQLLEAQLNQVNDDGRSSTPSSDSNDVLSESDDDMDDELSTSSDEDEEVDADVGLENSPASTEATPTDESLNLIQLKEKYGHFNGSSTVYDSRNKDEKFPTLDKHESSSSESSVMTGEESSIYSSSENESQNENDRESDDKTPSVGLSALFGKGEESDGDLDLDDSEDFTVNSSSVEGEELEKDQVDNSAATFERAGDFVHTQNENRDDIKDVEEDAETKVQEEQLSVVDVPVPSLLRGNLRTYQKQGLNWLASLYNNHTNGILADEMGLGKTIQTISLLAYLACEKENWGPHLIVVPTSVLLNWEMEFKRFAPGFKVLTYYGSPQQRKEKRKGWNKPDAFHVCIVSYQLVVQDQHSFKRKRWQYMVLDEAHNIKNFRSTRWQALLNFNTQRRLLLTGTPLQNNLAELWSLLYFLMPQTVIDGKKVSGFADLDAFQQWFGRPVDKIIETGQNFGQDKETKKTVAKLHQVLRPYLLRRLKADVEKQMPAKYEHIVYCKLSKRQRFLYDDFMSRAQTKATLASGNFMSIVNCLMQLRKVCNHPNLFEVRPILTSFVLEHCVASDYKDVERTLLKLFKKNNQVNRVDLDFLNLVFTLNDKDLTSYHAEEISKLTCVKNFVEEVNKLRETNKQLQEEFGEASFLNFQDANQYFKYSNKQKLEGTVDMLNFLKMVNKLRCDRRPIFGKNLIDLLTKDRRVKYDKSSIIDNELIKPLQTRVLDNRKIIDTFAVLTPSAVSLDMRKLALGLNDDSSVGENTRLKVMQNCFEVSNPLHQLQTKLTIAFPDKSLLQYDCGKLQKLAILLQQLKDNGHRALIFTQMTKVLDVLEQFLNYHGYLYMRLDGATKIEDRQILTERFNTDSRITVFILSSRSGGLGINLTGADTVIFYDSDWNPAMDKQCQDRCHRIGQTRDVHIYRFVSEHTIESNILKKANQKRQLDNVVIQEGDFTTDYFSKLSVRDLLGSELPENASGGDKPLIADADVAAKDPRQLERLLAQAEDEDDVKAANLAMREVEIDNDDFDESTEKKAANEEEENHAELDEYEGTAHVDEYMIRFIANGYYY 156E3BB5978905C8 156E3BB5978905C8 vps72_yeast Vacuolar protein sorting-associated protein 72 SWR complex protein 2 VPS72 SWC2 YDR485C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSDEGADKSLDTNTEFIIQTRSRRSNAGNKLQKLLEQELRDIESTKRQISSYKNGNDDEEDEIGLLFQEDEDDEDFEMMAKDDDDEGEEKEDETQSIRKEPSQASSEQAADDLMFSSSESEDSSNENDEDAEEKEIRRQELLSRKKRNKRLQKGPVVIKKQKPKPKSGEAIPRSHHTHEQLNAETLLLNTRRTSKRSSVMENTMKVYEKLSKAEKKRKIIQERIRKHKEQESQHMLTQEERLRIAKETEKLNILSLDKFKEQEVWKKENRLALQKRQKQKFQPNETILQFLSTAWLMTPAMELEDRKYWQEQLNKRDKKKKKYPRKPKKNLNLGKQDASDDKKRESEESIKNDGDVNSLGENSSSVHNQKRIEETSTNDTVEGESSPDAAVSRVNSDELKPTALPDVTLDAIANKQSTVDEAPNSQPQKNIITNEQKITNVGEPIQNLHNEEIKDEMVSALESRENTFENSSPAAQVVSQRDNSATPTPSNSTGTEDTILISPDTDIKGEPEPCLKTEGIENLSHNVPQETKSNTDVSFLKQVTFTDHPQVAIIDTEESPSKKDTANVDESSAENSLSTQTYEGPEQLTSRNFVTLYDFPNAPPNLKDFNTNLFGDRWSYTNGLSATQRPQDMKTVFHSILPSPPQSSVPSPTVDISLDLSALANFPSFGEYDKKIVHQINTEINKDLEIKIKTQPPTGVFLANGIRKKCLITNKECQYFDPRTGVPYSDVEAYKIIQRIQDPISKEEGRSDIKRDETTNEDSDDQVRFKWFGFKNGGIYLDLSQRPAKGVPEGF 6D3053EBB610DB95 6D3053EBB610DB95 act_yeast Actin ACT1 ABY1 END7 YFL039C protein protein yeast Saccharomyces cerevisiae Baker's yeast MDSEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGIMVGMGQKDSYVGDEAQSKRGILTLRYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPMNPKSNREKMTQIMFETFNVPAFYVSIQAVLSLYSSGRTTGIVLDSGDGVTHVVPIYAGFSLPHAILRIDLAGRDLTDYLMKILSERGYSFSTTAEREIVRDIKEKLCYVALDFEQEMQTAAQSSSIEKSYELPDGQVITIGNERFRAPEALFHPSVLGLESAGIDQTTYNSIMKCDVDVRKELYGNIVMSGGTTMFPGIAERMQKEITALAPSSMKVKIIAPPERKYSVWIGGSILASLTTFQQMWISKQEYDESGPSIVHHKCF 87AC19B0B0BC9E71 87AC19B0B0BC9E71 ies6_yeast Chromatin-remodeling complex subunit IES6 YEL044W SYGP-ORF13 IES6 Ino eighty subunit 6 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSGSRGNSSNSSVSNNSNNNNNNDGGDERLLFLRSVGERNEIGFPSRFKSAHYKKPTRRHKSARQLISDENKRINALLTKANKAAESSTAARRLVPKATYFSVEAPPSIRPAKKYCDVTGLKGFYKSPTNNIRYHNAEIYQLIVKPMAPGVDQEYLKLRGANFVLK 4B074DB4B10DC044 arp4_yeast Actin-related protein 4 ARP4 ACT3 YJL081C J1012 Actin-like protein ARP4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNAALQVYGGDEVSAVVIDPGSYTTNIGYSGSDFPQSILPSVYGKYTADEGNKKIFSEQSIGIPRKDYELKPIIENGLVIDWDTAQEQWQWALQNELYLNSNSGIPALLTEPVWNSTENRKKSLEVLLEGMQFEACYLAPTSTCVSFAAGRPNCLVVDIGHDTCSVSPIVDGMTLSKSTRRNFIAGKFINHLIKKALEPKEIIPLFAIKQRKPEFIKKTFDYEVDKSLYDYANNRGFFQECKETLCHICPTKTLEETKTELSSTAKRSIESPWNEEIVFDNETRYGFAEELFLPKEDDIPANWPRSNSGVVKTWRNDYVPLKRTKPSGVNKSDKKVTPTEEKEQEAVSKSTSPAANSADTPNETGKRPLEEEKPPKENNELIGLADLVYSSIMSSDVDLRATLAHNVVLTGGTSSIPGLSDRLMTELNKILPSLKFRILTTGHTIERQYQSWLGGSILTSLGTFHQLWVGKKEYEEVGVERLLNDRFR 6DE1C8938C6C3DBB 6DE1C8938C6C3DBB swc3_yeast SWR1-complex protein 3 SWC3 FUN36 SWC1 YAL011W protein protein yeast Saccharomyces cerevisiae Baker's yeast MPAVLRTRSKESSIEQKPASRTRTRSRRGKRGRDDDDDDDDEESDDAYDEVGNDYDEYASRAKLATNRPFEIVAGLPASVELPNYNSSLTHPQSIKNSGVLYDSLVSSRRTWVQGEMFELYWRRPKKIVSESTPAATESPTSGTIPLIRDKMQKMCDCVMSGGPHTFKVRLFILKNDKIEQKWQDEQELKKKEKELKRKNDAEAKRLRMEERKRQQMQKKIAKEQKLQLQKENKAKQKLEQEALKLKRKEEMKKLKEQNKNKQGSPSSSMHDPRMIMNLNLMAQEDPKLNTLMETVAKGLANNSQLEEFKKFIEIAKKRSLEENPVNKRPSVTTTRPAPPSKAKDVAEDHRLNSITLVKSSKTAATEPEPKKADDENAEKQQSKEAKTTAESTQVDVKKEEEDVKEKGVKSEDTQKKEDNQVVPKRKRRKNAIKEDKDMQLTAFQQKYVQGAEIILEYLEFTHSRYYLPKKSVVEFLEDTDEIIISWIVIHNSKEIEKFKTKKIKAKLKADQKLNKEDAKPGSDVEKEVSFNPLFEADCPTPLYTPMTMKLSGIHKRFNQIIRNSVSPMEEVVKEMEKILQIGTRLSGYNLWYQLDGYDDEALSESLRFELNEWEHAMRSRRHKR A4F13DB85B657D59 A4F13DB85B657D59 ies3_yeast Ino eighty subunit 3 IES3 L2131 YLR052W protein protein yeast Saccharomyces cerevisiae Baker's yeast MKFEDLLATNKQVQFAHAATQHYKSVKTPDFLEKDPHHKKFHNADGLNQQGSSTPSTATDANAASTASTHTNTTTFKRHIVAVDDISKMNYEMIKNSPGNVITNANQDEIDISTLKTRLYKDNLYAMNDNFLQAVNDQIVTLNAAEQDQETEDPDLSDDEKIDILTKIQENLLEEYQKLSQKERKWFILKELLLDANVELDLFSNRGRKASHPIAFGAVAIPTNVNANSLAFNRTKRRKINKNGLLENIL 5A0A2229D6C87ABF 5A0A2229D6C87ABF arp6_yeast Actin-like protein ARP6 ARP6 L2393 L9449.13 YLR085C protein protein yeast Saccharomyces cerevisiae Baker's yeast METPPIVIDNGSYEIKFGPSTNKKPFRALNALAKDKFGTSYLSNHIKNIKDISSITFRRPHELGQLTLWELESCIWDYCLFNPSEFDGFDLKEGKGHHLVASESCMTLPELSKHADQVIFEEYEFDSLFKSPVAVFVPFTKSYKGEMRTISGKDEDIDIVRGNSDSTNSTSSESKNAQDSGSDYHDFQLVIDSGFNCTWIIPVLKGIPYYKAVKKLDIGGRFLTGLLKETLSFRHYNMMDETILVNNIKEQCLFVSPVSYFDSFKTKDKHALEYVLPDFQTSFLGYVRNPRKENVPLPEDAQIITLTDELFTIPETFFHPEISQITKPGIVEAILESLSMLPEIVRPLMVGNIVCTGGNFNLPNFAQRLAAELQRQLPTDWTCHVSVPEGDCALFGWEVMSQFAKTDSYRKARVTREEYYEHGPDWCTKHRFGYQNWI 92D201F62D04232B 92D201F62D04232B vps71_yeast Vacuolar protein sorting-associated protein 71 YML041C YM8054.02C VPS71 SWC6 SWR complex protein 6 protein protein yeast Saccharomyces cerevisiae Baker's yeast MKALVEEIDKKTYNPDIYFTSLDPQARRYTSKKINKQGTISTSRPVKRINYSLADLEARLYTSRSEGDGNSISRQDDRNSKNSHSFEERYTQQEILQSDRRFMELNTENFSDLPNVPTLLSDLTGVPRDRIESTTKPISQTSDGLSALMGGSSFVKEHSKYGHGWVLKPETLREIQLSYKSTKLPKPKRKNTNRIVALKKVLSSKRNLHSFLDSALLNLMDKNVIYHNVYNKRYFKVLPLITTCSICGGYDSISSCVNCGNKICSVSCFKLHNETRCRNR D95B57C72ACB648A D95B57C72ACB648A swc4_yeast SWR1-complex protein 4 SWC4 EAF2 GOD1 ESA1-associated factor 2 YGR002C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSSDIFDVLNIKQKSRSPTNGQVSVPSSSAANRPKPQVTGMQRELFNLLGENQPPVVIKSGNNFKEKMLSTSKPSPWSFVEFKANNSVTLRHWVKGSKELIGDTPKESPYSKFNQHLSIPSFTKEEYEAFMNENEGTQKSVESEKNHNENFTNEKKDESKNSWSFEEIEYLFNLCKKYDLRWFLIFDRYSYNNSRTLEDLKEKFYYTCRNYFKASDPSNPLLSSLNFSAEKEIERKKYLQRLLSRSAAEIAEEEALVVESKKFEMAAKRTLAERESLLRLLDSPHSDQTITQYLTSQGMSQLYNALLADKTRKRKHDLNIPENPWMKQQQQFAQHRQLQQLNVKKSEVKENLSPKKTKRQRQEMQTALKRKSESAYAEQLLKDFNSDERKALGVITHGEKLSPGVYLRSTKLSTFKPALQNKILAILQELSLPSRPVMPSFDVMERQEELLKKINTLIDLKKHVDKYEAGMSITK 512CB8DF3171E7A3 512CB8DF3171E7A3 nhp10_yeast Non-histone protein 10 HMO2 YD8119.05C YDL002C NHP10 High mobility group protein 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSVEEKKRRLEELKDQNVVLGLAIQRSRLSVKRLKLEYGVLLERLESRIELDPELNCEDPLPTLASFKQELLTKPFRKSKTKRHKVKERDPNMPKRPTNAYLLYCEMNKERIRQNGSLDVTRDLAEGWKNLNEQDRKPYYKLYSEDRERYQMEMEIYNKKISNIDADDDKEENEQKIKNNEEGSSTKVADSKGGEDGSLVSSN EE6F488F78D32415 ruvb1_yeast RuvB-like protein 1 RVB1 TIP49-homology protein 1 TIP49a homolog TIH1 TIP49A YDR190C protein protein yeast Saccharomyces cerevisiae Baker's yeast MVAISEVKENPGVNSSNSGAVTRTAAHTHIKGLGLDESGVAKRVEGGFVGQIEAREACGVIVDLIKAKKMSGRAILLAGGPSTGKTALALAISQELGPKVPFCPLVGSELYSVEVKKTETLMENFRRAIGLRIKETKEVYEGEVTELTPEDAENPLGGYGKTISHVIVGLKSAKGTKTLRLDPTIYESIQREKVSIGDVIYIEANTGAVKRVGRSDAYATEFDLETEEYVPLPKGEVHKKKEIVQDVTLHDLDVANARPQGGQDVISMMGQLLKPKKTEITEKLRQEVNKVVAKYIDQGVAELIPGVLFIDEVNMLDIEIFTYLNKALESNIAPVVVLASNRGMTTVRGTEDVISPHGVPPDLIDRLLIVRTLPYDKDEIRTIIERRATVERLQVESSALDLLATMGTETSLRYALQLLAPCGILAQTSNRKEIVVNDVNEAKLLFLDAKRSTKILETSANYL 93D6ECB7006AF0F2 93D6ECB7006AF0F2 ies2_yeast Ino eighty subunit 2 N1315 YNL215W IES2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDSEASDIEAELSDSVSAGGEEYIDDDDYTEDIDDQIVTAKSSRRTARRSVPKGVRTSKRIRDKELSVEVDEDYDEEEDVLSPSKKRHLHTRSMDKRQVAATASEKSDIGDSKGNDGEIEDGILEEEESLEKELNRGGGKEVEKSEESYYAQNDVGQKGEEEQDGESGGYEDNEPSISKESDELVSVVNGNGNEEDDEVEATKENTTDSTRSTTTRSKMLLDLLEDGGSKKKLTDEEIQLRRAENARKRKNLSEKRLEEEKQDTINKLLKKRAGKSRSHLPNDDEKNDGSSSFVKPRRPYNSEGMTRILRRYEEDLFCTF 6F0AA04387A3B3E8 6F0AA04387A3B3E8 bdf1_yeast Bromodomain-containing factor 1 L8084.18 YLR399C BDF1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MTDITPVQNDVDVNGNNVNDDVSSNLKRPIDQGDPSNGLAEEENPANNQLHLKKARLDGDALTSSPAGLAENGIEGATLAANGENGYNATGSGAEDEQQGLKKEEGGQGTKQEDLDENSKQELPMEVPKEPAPAPPPEPDMNNLPQNPIPKHQQKHALLAIKAVKRLKDARPFLQPVDPVKLDIPFYFNYIKRPMDLSTIERKLNVGAYEVPEQITEDFNLMVNNSIKFNGPNAGISQMARNIQASFEKHMLNMPAKDAPPVIAKGRRSSAQEDAPIVIRRAQTHNGRPKRTIHPPKSKDIYPYESKKPKSKRLQQAMKFCQSVLKELMAKKHASYNYPFLEPVDPVSMNLPTYFDYVKEPMDLGTIAKKLNDWQYQTMEDFERDVRLVFKNCYTFNPDGTIVNMMGHRLEEVFNSKWADRPNLDDYDSDEDSRTQGDYDDYESEYSESDIDETIITNPAIQYLEEQLARMKVELQQLKKQELEKIRKERRLARGSKKRGKRSKGRSGSKNASSKGRRDKKNKLKTVVTYDMKRIITERINDLPTSKLERAIDIIKKSMPNISEDDEVELDLDTLDNHTILTLYNTFFRQYESSSGASNGLDGTSGVTRDASSLSPTSAGSRKRRSKALSQEEQSRQIEKIKNKLAILDSASPLSQNGSPGQIQSAAHNGFSSSSDDDVSSESEEE 8CCD52F41F91D0DA 8CCD52F41F91D0DA swc5_yeast SWR1-complex protein 5 YBR231C YBR1529 SWC5 AOR1 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPEVETKIIPNEKEDEDEDGYIEEEDEDFQPEKDKLGGGSDDSDASDGGDDYDDGVNRDKGRNKVDYSRIESESGGLIKTRRARQAEEEYAKTHKYESLTVESIPAKVNSIWEELQEASKNRLLSSSGKVGSVLDGSKEARSTTAAQQEDKILIERNYKFAGETVHEKKWVSRSSAEGQEYLNSLKFKQQAPAAPVQLEKAVRTKSNESRQHLRRPLKRPPLLEQIISGGLRPKLTTLEKSQLDWASYVDRAGLNDELVLHNKDGFLARQEFLQRVGSAEDERYKELRRQQLAQQLQQDSEAS 985F66B3E3CB3F25 985F66B3E3CB3F25 ies4_yeast Ino eighty subunit 4 YOR189W IES4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQESSVLSESQEQLANNPKIEDTSPPSANSRDNSKPVLPWDYKNKAIEIKSFSGYKVNFTGWIRRDVREERQRGSEFTASDVKGSDDKATRKKEPADEDPEVKQLEKEGEDGLDS 554493F28D1151C0 ino80_yeast Chromatin-remodeling ATPase INO80 YGL150C G1880 INO80 Inositol-requiring protein 80 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLAVLLNKEDKDISDFSKTTAGKSAKKNSRERVADVAPTRVLDKKQAYLSQLNSEFNRIKRRDSIEQLYQDWKFINLQEFELISEWNQQSKDWQFDNTNDSQDLHFKKLYRDMSMINKEWAEYQSFKNANLSDIINEKDADEDEEDDEDELEDGEEDMEEDEASTGRHTNGKSMRGNGIQKSRKKDAAAAAAIGKAIKDDQTHADTVVTVNGDENEDGNNGEDEDNDNDNENNNDNDNDNENENDNDSDNDDEEENGEEDEEEEEIEDLDEEDFAAFEEQDDNDDEDFNPDVEKRRKRSSSSSSSTKLSMNSLSLITSKKINKNITINSDRPKIVRELIKMCNKNKHQKIKKRRFTNCIVTDYNPIDSKLNIKITLKQYHVKRLKKLINDAKREREREEALKNNVGLDGNDLDNDEDGSESHKRRKLNNNTANGADDANKRKFNTRHGLPTYGMKMNAKEARAIQRHYDNTYTTIWKDMARKDSTKMSRLVQQIQSIRSTNFRKTSSLCAREAKKWQSKNFKQIKDFQTRARRGIREMSNFWKKNEREERDLKKKIEKEAMEQAKKEEEEKESKRQAKKLNFLLTQTELYSHFIGRKIKTNELEGNNVSSNDSESQKNIDISALAPNKNDFHAIDFDNENDEQLRLRAAENASNALAETRAKAKQFDDHANAHEEEEEEDELNFQNPTSLGEITIEQPKILACTLKEYQLKGLNWLANLYDQGINGILADEMGLGKTVQSISVLAHLAENHNIWGPFLVVTPASTLHNWVNEISKFLPQFKILPYWGNANDRKVLRKFWDRKNLRYNKNAPFHVMVTSYQMVVTDANYLQKMKWQYMILDEAQAIKSSQSSRWKNLLSFHCRNRLLLTGTPIQNSMQELWALLHFIMPSLFDSHDEFNEWFSKDIESHAEANTKLNQQQLRRLHMILKPFMLRRVKKNVQSELGDKIEIDVLCDLTQRQAKLYQVLKSQISTNYDAIENAATNDSTSNSASNSGSDQNLINAVMQFRKVCNHPDLFERADVDSPFSFTTFGKTTSMLTASVANNNSSVISNSNMNLSSMSSNNISNGKFTDLIYSSRNPIKYSLPRLIYEDLILPNYNNDVDIANKLKNVKFNIFNPSTNYELCLFLSKLTGEPSLNEFFRVSTTPLLKRVIERTNGPKNTDSLSFKTITQELLEVTRNAPSEGVMASLLNVEKHAYEREYLNCIQRGYHPNVSAPPVTIEVLGSSHVTNSINNELFDPLISQALSDIPAITQYNMHVKKGIPVEDFPKTGLFPEPLNKNFSSNISMPSMDRFITESAKLRKLDELLVKLKSEGHRVLIYFQMTKMMDLMEEYLTYRQYNHIRLDGSSKLEDRRDLVHDWQTNPEIFVFLLSTRAGGLGINLTAADTVIFYDSDWNPTIDSQAMDRAHRLGQTRQVTVYRLLVRGTIEERMRDRAKQKEQVQQVVMEGKTQEKNIKTIEVGENDSEVTREGSKSISQDGIKEAASALA 017053C5A245337E 017053C5A245337E ies1_yeast Ino eighty subunit 1 IES1 YFL013C protein protein yeast Saccharomyces cerevisiae Baker's yeast MGKRVYDPIHDTFQLREDNSDETKADSPMQSVKSGSQEEASPSSIQSETETVTTKSIPVIHEIEIDDKNDDDSTQSEEENTNILLNFEPSTVPEATGASTATGPVTTNTVRRKPKESNASKYNRHLKKPDGEPFNRKDIQFSFMQELLMDKRQIFTNVLKPLYKNSIVPINIDGDKLSINVTDKEYDARTFVFNDKLTFAQLYVLTIATSIKCSKILRDKLLLDQQVAFSTCVLALLVNIGRLNTTINFYLEMTSQLRTFHSVPVLQLHANDPKLLQDTPRLKSILKNLPWGNEQLSLMETYKKVDQNDGEVDTVNKFNIINMLFSICDNSGLIDKRFLSKYVEVESKAQEQDMVDEQNEVKETEAENEKQESKAAYATTLFDILDYSKYEPKDRSNILIWLLYIHLETNLSQEEVEESVRFFNGLEDGAPAGKFILRCTERSYDTDPEDELEFGANQRIKRREFMSKMEEGRKRERTNVTEVKKPSIGGDKSEEDGEGEDDKSEETVEETRSLLTPTPILESSSPMTLNRKKVTPQLPKVTPAAPTETEEEITSAAIIDKNDLNLTPLKKYNSSATVNKVDKLISLDLNKHVSENGKTQEEFLADLKKSQVPNRLKRRDIGLIKIFNEFEDIPVASVLGIRGKKRKKFKDNLLGFETDFMKNLGASKKVLLNKIERAEIDDEEATAMFKLE 9C0084A36431B241 9C0084A36431B241 ies5_yeast Ino eighty subunit 5 YER092W IES5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPSKDPESVIDKEIRKISARNDELIKQDGTLKREYTTLLRKVSSVITVLNSIDDADTGSAETELPRLISQATVEKVPELKWYNDQISLITEKLEDDEDIEVPEELMDAYTLYKETPLLYNDTHTP 777B96A00E9FF6E2 swc7-act1 2 27 unspecified role unspecified role neutral component neutral component 22 unspecified role unspecified role neutral component neutral component 19 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component 14 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 21 unspecified role unspecified role neutral component neutral component 23 unspecified role unspecified role neutral component neutral component 16 unspecified role unspecified role neutral component neutral component 28 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component 25 unspecified role unspecified role neutral component neutral component 18 unspecified role unspecified role neutral component neutral component physical association physical association Fig. 3, Supp. Fig.1 Stoichiometry estimated from band intensity/ act1-arp4-7 4 16 unspecified role unspecified role neutral component neutral component 21 unspecified role unspecified role neutral component neutral component 23 unspecified role unspecified role neutral component neutral component 28 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component 25 unspecified role unspecified role neutral component neutral component 18 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component 14 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 22 unspecified role unspecified role neutral component neutral component 27 unspecified role unspecified role neutral component neutral component 19 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component physical association physical association Fig. 1D, 1E,Supp. Fig.1B 28A act1-arp4-8 7 13 unspecified role unspecified role neutral component neutral component 16 unspecified role unspecified role neutral component neutral component 19 unspecified role unspecified role neutral component neutral component 18 unspecified role unspecified role neutral component neutral component 25 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component 14 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component 22 unspecified role unspecified role neutral component neutral component 21 unspecified role unspecified role neutral component neutral component 27 unspecified role unspecified role neutral component neutral component 23 unspecified role unspecified role neutral component neutral component 28 unspecified role unspecified role neutral component neutral component physical association physical association Fig. 3 Stoichiometry estimated from band intensity/ arp4-arp5-2 5 30 unspecified role unspecified role neutral component neutral component 24 unspecified role unspecified role neutral component neutral component 26 unspecified role unspecified role neutral component neutral component 20 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component 25 unspecified role unspecified role neutral component neutral component 17 unspecified role unspecified role neutral component neutral component 32 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component 18 unspecified role unspecified role neutral component neutral component 31 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component 29 unspecified role unspecified role neutral component neutral component association association Supp. Fig 4 act1-arp4-9 1 31 unspecified role unspecified role neutral component neutral component 20 unspecified role unspecified role neutral component neutral component 18 unspecified role unspecified role neutral component neutral component 32 unspecified role unspecified role neutral component neutral component 26 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component 24 unspecified role unspecified role neutral component neutral component 29 unspecified role unspecified role neutral component neutral component 25 unspecified role unspecified role neutral component neutral component 9 unspecified role unspecified role neutral component neutral component 17 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component 30 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component 16 unspecified role unspecified role neutral component neutral component association association Supp. Fig 4 act1-arp4-11 6 14 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 16 unspecified role unspecified role neutral component neutral component 18 unspecified role unspecified role neutral component neutral component 25 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component 28 unspecified role unspecified role neutral component neutral component 23 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component 27 unspecified role unspecified role neutral component neutral component physical association physical association Supp. Fig. 5A Swc7 assumed to also be in complex in subsequent experiments but is not labelled as present on this gel. act1-arp4-14 4 25 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component 14 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 28 unspecified role unspecified role neutral component neutral component 16 unspecified role unspecified role neutral component neutral component 18 unspecified role unspecified role neutral component neutral component 27 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component 23 unspecified role unspecified role neutral component neutral component physical association physical association Fig.4C, Supp. Fig. 5B act1-arp4-13 6 14 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 25 unspecified role unspecified role neutral component neutral component 19 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component 22 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component physical association physical association Supp. Fig.5C rvb1-rvb2-10 4 22 unspecified role unspecified role neutral component neutral component 14 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role neutral component neutral component 19 unspecified role unspecified role neutral component neutral component 15 unspecified role unspecified role neutral component neutral component 25 unspecified role unspecified role neutral component neutral component physical association physical association Fig.4C, Supp. Fig.5D Stoichiometry estimated from band intensity/ act1-arp4-15 2 25 unspecified role unspecified role neutral component neutral component 18 unspecified role unspecified role neutral component neutral component 16 unspecified role unspecified role neutral component neutral component 27 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component 28 unspecified role unspecified role neutral component neutral component 23 unspecified role unspecified role neutral component neutral component 14 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role neutral component neutral component physical association physical association Fig. 4B rvb1-rvb2-11 2 12 unspecified role unspecified role neutral component neutral component 22 unspecified role unspecified role neutral component neutral component 14 unspecified role unspecified role neutral component neutral component region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 15 unspecified role unspecified role neutral component neutral component 19 unspecified role unspecified role neutral component neutral component 25 unspecified role unspecified role neutral component neutral component physical association physical association Fig.4B Stoichiometry estimated from band intensity/ act1-arp4-16 3 27 unspecified role unspecified role neutral component neutral component lys-504 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component lys-123 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-331 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 18 unspecified role unspecified role neutral component neutral component lys-195 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-323 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-335 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 22 unspecified role unspecified role neutral component neutral component 19 unspecified role unspecified role neutral component neutral component lys-407 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-498 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 25 unspecified role unspecified role neutral component neutral component lys-42 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-31 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-454 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 28 unspecified role unspecified role neutral component neutral component lys-205 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-138 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-205 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-138 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-205 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 16 unspecified role unspecified role neutral component neutral component lys-50 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-61 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 15 unspecified role unspecified role neutral component neutral component lys-218 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-228 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 21 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component lys-219 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 14 unspecified role unspecified role neutral component neutral component lys-418 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-371 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-722 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-1456 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-326 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 23 unspecified role unspecified role neutral component neutral component lys-262 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-238 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 171 198 199 174 175 187 177 183 212 178 186 179 182 206 185 204 207 189 202 190 191 195 192 208 193 196 205 211 physical association physical association Fig. 5, Supp. Table 2