IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structure and subunit topology of the INO80 chromatin remodeler and its nucleosome complex. Structure and subunit topology of the INO80 chromatin remodeler and its nucleosome complex. Cell (0092-8674) 2013 imex curation Tosi A., Haas C., Herzog F., Gilmozzi A., Berninghausen O., Ungewickell C., Gerhold C.B., Lakomek K., Aebersold R., Beckmann R., Hopfner K.P. beckmann@genzentrum.lmu.de, hopfner@genzentrum.lmu.de 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast pull down pull down anti tag western anti tag western blot anti tag western Tosi A., Haas C., Herzog F., Gilmozzi A., Berninghausen O., Ungewickell C., Gerhold C.B., Lakomek K., Aebersold R., Beckmann R., Hopfner K.P. beckmann@genzentrum.lmu.de, hopfner@genzentrum.lmu.de Accepted 2013-NOV-18 by MGT 22-Nov-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation Structure and subunit topology of the INO80 chromatin remodeler and its nucleosome complex. Structure and subunit topology of the INO80 chromatin remodeler and its nucleosome complex. Cell (0092-8674) 2013 imex curation Tosi A., Haas C., Herzog F., Gilmozzi A., Berninghausen O., Ungewickell C., Gerhold C.B., Lakomek K., Aebersold R., Beckmann R., Hopfner K.P. beckmann@genzentrum.lmu.de, hopfner@genzentrum.lmu.de 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. chromatography chromatography technology column chromatography chromatography weight by comassie molecular weight estimation by coomasie staining weight by comassie Tosi A., Haas C., Herzog F., Gilmozzi A., Berninghausen O., Ungewickell C., Gerhold C.B., Lakomek K., Aebersold R., Beckmann R., Hopfner K.P. No details given as to how this complex was purified. beckmann@genzentrum.lmu.de, hopfner@genzentrum.lmu.de Accepted 2013-NOV-18 by MGT 22-Nov-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation Structure and subunit topology of the INO80 chromatin remodeler and its nucleosome complex. Structure and subunit topology of the INO80 chromatin remodeler and its nucleosome complex. Cell (0092-8674) 2013 imex curation Tosi A., Haas C., Herzog F., Gilmozzi A., Berninghausen O., Ungewickell C., Gerhold C.B., Lakomek K., Aebersold R., Beckmann R., Hopfner K.P. beckmann@genzentrum.lmu.de, hopfner@genzentrum.lmu.de 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink sequence tag sequence tag identification sequence tag Tosi A., Haas C., Herzog F., Gilmozzi A., Berninghausen O., Ungewickell C., Gerhold C.B., Lakomek K., Aebersold R., Beckmann R., Hopfner K.P. Crosslinked with an equimolar mixture of d0- and d12-labeled disuccinimidyl suberate beckmann@genzentrum.lmu.de, hopfner@genzentrum.lmu.de Accepted 2013-NOV-18 by MGT 22-Nov-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation Structure and subunit topology of the INO80 chromatin remodeler and its nucleosome complex. Structure and subunit topology of the INO80 chromatin remodeler and its nucleosome complex. Cell (0092-8674) 2013 imex curation Tosi A., Haas C., Herzog F., Gilmozzi A., Berninghausen O., Ungewickell C., Gerhold C.B., Lakomek K., Aebersold R., Beckmann R., Hopfner K.P. beckmann@genzentrum.lmu.de, hopfner@genzentrum.lmu.de 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast crosslink cross-linking study crosslink sequence tag sequence tag identification sequence tag Tosi A., Haas C., Herzog F., Gilmozzi A., Berninghausen O., Ungewickell C., Gerhold C.B., Lakomek K., Aebersold R., Beckmann R., Hopfner K.P. Crosslinked with an equimolar mixture of d0- and d12-labeled disuccinimidyl suberate beckmann@genzentrum.lmu.de, hopfner@genzentrum.lmu.de Accepted 2013-NOV-18 by MGT 22-Nov-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation Structure and subunit topology of the INO80 chromatin remodeler and its nucleosome complex. Structure and subunit topology of the INO80 chromatin remodeler and its nucleosome complex. Cell (0092-8674) 2013 imex curation Tosi A., Haas C., Herzog F., Gilmozzi A., Berninghausen O., Ungewickell C., Gerhold C.B., Lakomek K., Aebersold R., Beckmann R., Hopfner K.P. beckmann@genzentrum.lmu.de, hopfner@genzentrum.lmu.de 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast electron microscopy electron microscopy Electron crystallography Electron cryomicroscopy Electron crystallography Electron cryomicroscopy predetermined predetermined participant predetermined Tosi A., Haas C., Herzog F., Gilmozzi A., Berninghausen O., Ungewickell C., Gerhold C.B., Lakomek K., Aebersold R., Beckmann R., Hopfner K.P. I've only included the DID tag data (Fig. 5A) as a comment as the structrues were not deposited, and what was in each complex is not clear. Both negative-stain (EMB-2385) and cryo-EM (EMD-2386) structures were obtained. beckmann@genzentrum.lmu.de, hopfner@genzentrum.lmu.de Accepted 2013-NOV-18 by MGT 22-Nov-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation Structure and subunit topology of the INO80 chromatin remodeler and its nucleosome complex. Structure and subunit topology of the INO80 chromatin remodeler and its nucleosome complex. Cell (0092-8674) 2013 imex curation Tosi A., Haas C., Herzog F., Gilmozzi A., Berninghausen O., Ungewickell C., Gerhold C.B., Lakomek K., Aebersold R., Beckmann R., Hopfner K.P. beckmann@genzentrum.lmu.de, hopfner@genzentrum.lmu.de 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Tosi A., Haas C., Herzog F., Gilmozzi A., Berninghausen O., Ungewickell C., Gerhold C.B., Lakomek K., Aebersold R., Beckmann R., Hopfner K.P. FLAG-elution was further purified to near homogeneity by MonoQ ion exchange and Superose 6 size exclusion chromatography beckmann@genzentrum.lmu.de, hopfner@genzentrum.lmu.de Accepted 2013-NOV-18 by MGT 22-Nov-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation Structure and subunit topology of the INO80 chromatin remodeler and its nucleosome complex. Structure and subunit topology of the INO80 chromatin remodeler and its nucleosome complex. Cell (0092-8674) 2013 imex curation Tosi A., Haas C., Herzog F., Gilmozzi A., Berninghausen O., Ungewickell C., Gerhold C.B., Lakomek K., Aebersold R., Beckmann R., Hopfner K.P. beckmann@genzentrum.lmu.de, hopfner@genzentrum.lmu.de 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions trini-high_5 Trichoplusia ni cell line from eggs high_5 Insect cell line from Trichoplusia ni egg cells Invitrogen supplies this cell line. Invitrogen supply this cell line. pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Tosi A., Haas C., Herzog F., Gilmozzi A., Berninghausen O., Ungewickell C., Gerhold C.B., Lakomek K., Aebersold R., Beckmann R., Hopfner K.P. beckmann@genzentrum.lmu.de, hopfner@genzentrum.lmu.de Accepted 2013-NOV-18 by MGT 22-Nov-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation ino80_yeast Chromatin-remodeling ATPase INO80 YGL150C G1880 INO80 Inositol-requiring protein 80 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLAVLLNKEDKDISDFSKTTAGKSAKKNSRERVADVAPTRVLDKKQAYLSQLNSEFNRIKRRDSIEQLYQDWKFINLQEFELISEWNQQSKDWQFDNTNDSQDLHFKKLYRDMSMINKEWAEYQSFKNANLSDIINEKDADEDEEDDEDELEDGEEDMEEDEASTGRHTNGKSMRGNGIQKSRKKDAAAAAAIGKAIKDDQTHADTVVTVNGDENEDGNNGEDEDNDNDNENNNDNDNDNENENDNDSDNDDEEENGEEDEEEEEIEDLDEEDFAAFEEQDDNDDEDFNPDVEKRRKRSSSSSSSTKLSMNSLSLITSKKINKNITINSDRPKIVRELIKMCNKNKHQKIKKRRFTNCIVTDYNPIDSKLNIKITLKQYHVKRLKKLINDAKREREREEALKNNVGLDGNDLDNDEDGSESHKRRKLNNNTANGADDANKRKFNTRHGLPTYGMKMNAKEARAIQRHYDNTYTTIWKDMARKDSTKMSRLVQQIQSIRSTNFRKTSSLCAREAKKWQSKNFKQIKDFQTRARRGIREMSNFWKKNEREERDLKKKIEKEAMEQAKKEEEEKESKRQAKKLNFLLTQTELYSHFIGRKIKTNELEGNNVSSNDSESQKNIDISALAPNKNDFHAIDFDNENDEQLRLRAAENASNALAETRAKAKQFDDHANAHEEEEEEDELNFQNPTSLGEITIEQPKILACTLKEYQLKGLNWLANLYDQGINGILADEMGLGKTVQSISVLAHLAENHNIWGPFLVVTPASTLHNWVNEISKFLPQFKILPYWGNANDRKVLRKFWDRKNLRYNKNAPFHVMVTSYQMVVTDANYLQKMKWQYMILDEAQAIKSSQSSRWKNLLSFHCRNRLLLTGTPIQNSMQELWALLHFIMPSLFDSHDEFNEWFSKDIESHAEANTKLNQQQLRRLHMILKPFMLRRVKKNVQSELGDKIEIDVLCDLTQRQAKLYQVLKSQISTNYDAIENAATNDSTSNSASNSGSDQNLINAVMQFRKVCNHPDLFERADVDSPFSFTTFGKTTSMLTASVANNNSSVISNSNMNLSSMSSNNISNGKFTDLIYSSRNPIKYSLPRLIYEDLILPNYNNDVDIANKLKNVKFNIFNPSTNYELCLFLSKLTGEPSLNEFFRVSTTPLLKRVIERTNGPKNTDSLSFKTITQELLEVTRNAPSEGVMASLLNVEKHAYEREYLNCIQRGYHPNVSAPPVTIEVLGSSHVTNSINNELFDPLISQALSDIPAITQYNMHVKKGIPVEDFPKTGLFPEPLNKNFSSNISMPSMDRFITESAKLRKLDELLVKLKSEGHRVLIYFQMTKMMDLMEEYLTYRQYNHIRLDGSSKLEDRRDLVHDWQTNPEIFVFLLSTRAGGLGINLTAADTVIFYDSDWNPTIDSQAMDRAHRLGQTRQVTVYRLLVRGTIEERMRDRAKQKEQVQQVVMEGKTQEKNIKTIEVGENDSEVTREGSKSISQDGIKEAASALA 017053C5A245337E 017053C5A245337E ies6_yeast Chromatin-remodeling complex subunit IES6 YEL044W SYGP-ORF13 IES6 Ino eighty subunit 6 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSGSRGNSSNSSVSNNSNNNNNNDGGDERLLFLRSVGERNEIGFPSRFKSAHYKKPTRRHKSARQLISDENKRINALLTKANKAAESSTAARRLVPKATYFSVEAPPSIRPAKKYCDVTGLKGFYKSPTNNIRYHNAEIYQLIVKPMAPGVDQEYLKLRGANFVLK 4B074DB4B10DC044 ies3_yeast Ino eighty subunit 3 IES3 L2131 YLR052W protein protein yeast Saccharomyces cerevisiae Baker's yeast MKFEDLLATNKQVQFAHAATQHYKSVKTPDFLEKDPHHKKFHNADGLNQQGSSTPSTATDANAASTASTHTNTTTFKRHIVAVDDISKMNYEMIKNSPGNVITNANQDEIDISTLKTRLYKDNLYAMNDNFLQAVNDQIVTLNAAEQDQETEDPDLSDDEKIDILTKIQENLLEEYQKLSQKERKWFILKELLLDANVELDLFSNRGRKASHPIAFGAVAIPTNVNANSLAFNRTKRRKINKNGLLENIL 5A0A2229D6C87ABF 5A0A2229D6C87ABF arp4_yeast Actin-related protein 4 ARP4 ACT3 YJL081C J1012 Actin-like protein ARP4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSNAALQVYGGDEVSAVVIDPGSYTTNIGYSGSDFPQSILPSVYGKYTADEGNKKIFSEQSIGIPRKDYELKPIIENGLVIDWDTAQEQWQWALQNELYLNSNSGIPALLTEPVWNSTENRKKSLEVLLEGMQFEACYLAPTSTCVSFAAGRPNCLVVDIGHDTCSVSPIVDGMTLSKSTRRNFIAGKFINHLIKKALEPKEIIPLFAIKQRKPEFIKKTFDYEVDKSLYDYANNRGFFQECKETLCHICPTKTLEETKTELSSTAKRSIESPWNEEIVFDNETRYGFAEELFLPKEDDIPANWPRSNSGVVKTWRNDYVPLKRTKPSGVNKSDKKVTPTEEKEQEAVSKSTSPAANSADTPNETGKRPLEEEKPPKENNELIGLADLVYSSIMSSDVDLRATLAHNVVLTGGTSSIPGLSDRLMTELNKILPSLKFRILTTGHTIERQYQSWLGGSILTSLGTFHQLWVGKKEYEEVGVERLLNDRFR 6DE1C8938C6C3DBB 6DE1C8938C6C3DBB nhp10_yeast Non-histone protein 10 HMO2 YD8119.05C YDL002C NHP10 High mobility group protein 2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSVEEKKRRLEELKDQNVVLGLAIQRSRLSVKRLKLEYGVLLERLESRIELDPELNCEDPLPTLASFKQELLTKPFRKSKTKRHKVKERDPNMPKRPTNAYLLYCEMNKERIRQNGSLDVTRDLAEGWKNLNEQDRKPYYKLYSEDRERYQMEMEIYNKKISNIDADDDKEENEQKIKNNEEGSSTKVADSKGGEDGSLVSSN EE6F488F78D32415 taf14_yeast Transcription initiation factor TFIID subunit 14 ANC1 CST10 YPL129W TAF14 SWP29 TAF30 TFG3 TBP-associated factor 14 TBP-associated factor 30 kDa Transcription initiation factor TFIIF 30 kDa subunit Transcription factor G 30 kDa subunit SWI/SNF chromatin-remodeling complex subunit TAF14 SWI/SNF complex subunit TAF14 SWI/SNF complex 29 kDa subunit Actin non-complementing mutant 1 Chromosome stability protein 10 protein protein yeast Saccharomyces cerevisiae Baker's yeast MVATVKRTIRIKTQQHILPEVPPVENFPVRQWSIEIVLLDDEGKEIPATIFDKVIYHLHPTFANPNRTFTDPPFRIEEQGWGGFPLDISVFLLEKAGERKIPHDLNFLQESYEVEHVIQIPLNKPLLTEELAKSGSTEETTANTGTIGKRRTTTNTTAEPKAKRAKTGSASTVKGSVDLEKLAFGLTKLNEDDLVGVVQMVTDNKTPEMNVTNNVEEGEFIIDLYSLPEGLLKSLWDYVKKNTE 60EA5EC2474B1B9C 60EA5EC2474B1B9C h3_drome Histone H3 His3 His3:CG31613 His3:CG33857 His3:CG33860 His3:CG33863 His3:CG33866 CG31613 CG33803 CG33806 CG33809 CG33812 CG33815 CG33818 CG33821 CG33824 CG33827 CG33830 CG33833 CG33836 CG33839 CG33842 CG33845 CG33848 CG33851 CG33854 CG33857 CG33860 CG33863 CG33866 His3:CG33803 His3:CG33806 His3:CG33809 His3:CG33812 His3:CG33815 His3:CG33818 His3:CG33821 His3:CG33824 His3:CG33827 His3:CG33830 His3:CG33833 His3:CG33836 His3:CG33839 His3:CG33842 His3:CG33845 His3:CG33848 His3:CG33851 His3:CG33854 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MARTKQTARKSTGGKAPRKQLATKAARKSAPATGGVKKPHRYRPGTVALREIRRYQKSTELLIRKLPFQRLVREIAQDFKTDLRFQSSAVMALQEASEAYLVGLFEDTNLCAIHAKRVTIMPKDIQLARRIRGERA DIP protein P02299 original sequence version: 100 6FD8508EA50A0EEC h2b_drome Histone H2B His2B His2B:CG17949 His2B:CG33868 His2B:CG33870 His2B:CG33872 His2B:CG33874 His2B:CG33876 His2B:CG33878 His2B:CG33880 His2B:CG33882 His2B:CG33884 His2B:CG33886 His2B:CG33888 His2B:CG33890 His2B:CG33892 His2B:CG33894 His2B:CG33896 His2B:CG33898 His2B:CG33900 His2B:CG33902 His2B:CG33904 His2B:CG33906 His2B:CG33908 His2B:CG33910 CG17949 CG33868 CG33870 CG33872 CG33874 CG33876 CG33878 CG33880 CG33882 CG33884 CG33886 CG33888 CG33890 CG33892 CG33894 CG33896 CG33898 CG33900 CG33902 CG33904 CG33906 CG33908 CG33910 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MPPKTSGKAAKKAGKAQKNITKTDKKKKRKRKESYAIYIYKVLKQVHPDTGISSKAMSIMNSFVNDIFERIAAEASRLAHYNKRSTITSREIQTAVRLLLPGELAKHAVSEGTKAVTKYTSSK DIP protein P02283 original sequence version: 88 0774D25F34003062 act_yeast Actin ACT1 ABY1 END7 YFL039C protein protein yeast Saccharomyces cerevisiae Baker's yeast MDSEVAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGIMVGMGQKDSYVGDEAQSKRGILTLRYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPMNPKSNREKMTQIMFETFNVPAFYVSIQAVLSLYSSGRTTGIVLDSGDGVTHVVPIYAGFSLPHAILRIDLAGRDLTDYLMKILSERGYSFSTTAEREIVRDIKEKLCYVALDFEQEMQTAAQSSSIEKSYELPDGQVITIGNERFRAPEALFHPSVLGLESAGIDQTTYNSIMKCDVDVRKELYGNIVMSGGTTMFPGIAERMQKEITALAPSSMKVKIIAPPERKYSVWIGGSILASLTTFQQMWISKQEYDESGPSIVHHKCF 87AC19B0B0BC9E71 87AC19B0B0BC9E71 ruvb1_yeast RuvB-like protein 1 RVB1 TIP49-homology protein 1 TIP49a homolog TIH1 TIP49A YDR190C protein protein yeast Saccharomyces cerevisiae Baker's yeast MVAISEVKENPGVNSSNSGAVTRTAAHTHIKGLGLDESGVAKRVEGGFVGQIEAREACGVIVDLIKAKKMSGRAILLAGGPSTGKTALALAISQELGPKVPFCPLVGSELYSVEVKKTETLMENFRRAIGLRIKETKEVYEGEVTELTPEDAENPLGGYGKTISHVIVGLKSAKGTKTLRLDPTIYESIQREKVSIGDVIYIEANTGAVKRVGRSDAYATEFDLETEEYVPLPKGEVHKKKEIVQDVTLHDLDVANARPQGGQDVISMMGQLLKPKKTEITEKLRQEVNKVVAKYIDQGVAELIPGVLFIDEVNMLDIEIFTYLNKALESNIAPVVVLASNRGMTTVRGTEDVISPHGVPPDLIDRLLIVRTLPYDKDEIRTIIERRATVERLQVESSALDLLATMGTETSLRYALQLLAPCGILAQTSNRKEIVVNDVNEAKLLFLDAKRSTKILETSANYL 93D6ECB7006AF0F2 93D6ECB7006AF0F2 ies2_yeast Ino eighty subunit 2 N1315 YNL215W IES2 protein protein yeast Saccharomyces cerevisiae Baker's yeast MDSEASDIEAELSDSVSAGGEEYIDDDDYTEDIDDQIVTAKSSRRTARRSVPKGVRTSKRIRDKELSVEVDEDYDEEEDVLSPSKKRHLHTRSMDKRQVAATASEKSDIGDSKGNDGEIEDGILEEEESLEKELNRGGGKEVEKSEESYYAQNDVGQKGEEEQDGESGGYEDNEPSISKESDELVSVVNGNGNEEDDEVEATKENTTDSTRSTTTRSKMLLDLLEDGGSKKKLTDEEIQLRRAENARKRKNLSEKRLEEEKQDTINKLLKKRAGKSRSHLPNDDEKNDGSSSFVKPRRPYNSEGMTRILRRYEEDLFCTF 6F0AA04387A3B3E8 6F0AA04387A3B3E8 ies1_yeast Ino eighty subunit 1 IES1 YFL013C protein protein yeast Saccharomyces cerevisiae Baker's yeast MGKRVYDPIHDTFQLREDNSDETKADSPMQSVKSGSQEEASPSSIQSETETVTTKSIPVIHEIEIDDKNDDDSTQSEEENTNILLNFEPSTVPEATGASTATGPVTTNTVRRKPKESNASKYNRHLKKPDGEPFNRKDIQFSFMQELLMDKRQIFTNVLKPLYKNSIVPINIDGDKLSINVTDKEYDARTFVFNDKLTFAQLYVLTIATSIKCSKILRDKLLLDQQVAFSTCVLALLVNIGRLNTTINFYLEMTSQLRTFHSVPVLQLHANDPKLLQDTPRLKSILKNLPWGNEQLSLMETYKKVDQNDGEVDTVNKFNIINMLFSICDNSGLIDKRFLSKYVEVESKAQEQDMVDEQNEVKETEAENEKQESKAAYATTLFDILDYSKYEPKDRSNILIWLLYIHLETNLSQEEVEESVRFFNGLEDGAPAGKFILRCTERSYDTDPEDELEFGANQRIKRREFMSKMEEGRKRERTNVTEVKKPSIGGDKSEEDGEGEDDKSEETVEETRSLLTPTPILESSSPMTLNRKKVTPQLPKVTPAAPTETEEEITSAAIIDKNDLNLTPLKKYNSSATVNKVDKLISLDLNKHVSENGKTQEEFLADLKKSQVPNRLKRRDIGLIKIFNEFEDIPVASVLGIRGKKRKKFKDNLLGFETDFMKNLGASKKVLLNKIERAEIDDEEATAMFKLE 9C0084A36431B241 9C0084A36431B241 ies4_yeast Ino eighty subunit 4 YOR189W IES4 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQESSVLSESQEQLANNPKIEDTSPPSANSRDNSKPVLPWDYKNKAIEIKSFSGYKVNFTGWIRRDVREERQRGSEFTASDVKGSDDKATRKKEPADEDPEVKQLEKEGEDGLDS 554493F28D1151C0 ruvb2_yeast RuvB-like protein 2 TIP49-homology protein 2 TIP49b homolog RVB2 TIH2 TIP49B YPL235W P1060 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSIQTSDPNETSDLKSLSLIAAHSHITGLGLDENLQPRPTSEGMVGQLQARRAAGVILKMVQNGTIAGRAVLVAGPPSTGKTALAMGVSQSLGKDVPFTAIAGSEIFSLELSKTEALTQAFRKSIGIKIKEETELIEGEVVEIQIDRSITGGHKQGKLTIKTTDMETIYELGNKMIDGLTKEKVLAGDVISIDKASGKITKLGRSFARSRDYDAMGADTRFVQCPEGELQKRKTVVHTVSLHEIDVINSRTQGFLALFTGDTGEIRSEVRDQINTKVAEWKEEGKAEIVPGVLFIDEVHMLDIECFSFINRALEDEFAPIVMMATNRGVSKTRGTNYKSPHGLPLDLLDRSIIITTKSYNEQEIKTILSIRAQEEEVELSSDALDLLTKTGVETSLRYSSNLISVAQQIAMKRKNNTVEVEDVKRAYLLFLDSARSVKYVQENESQYIDDQGNVQISIAKSADPDAMDTTE 0D8EFBA7EC711AB8 0D8EFBA7EC711AB8 ies5_yeast Ino eighty subunit 5 YER092W IES5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MPSKDPESVIDKEIRKISARNDELIKQDGTLKREYTTLLRKVSSVITVLNSIDDADTGSAETELPRLISQATVEKVPELKWYNDQISLITEKLEDDEDIEVPEELMDAYTLYKETPLLYNDTHTP 777B96A00E9FF6E2 arp5_yeast Actin-related protein 5 ARP5 YNL059C N2430 YNL2430C Actin-like protein ARP5 protein protein yeast Saccharomyces cerevisiae Baker's yeast MSSRDASLTPLKAVVIDDPPLRQTPEPFDEQSAYNPQSPIAIDFGSSKLRAGFVNHATPTHIFPNALTKFRDRKLNKNFTFVGNDTLLDQAVRSQSRSPFDGPFVTNWNLTEEILDYTFHHLGVVPDNGIPNPILLTERLATVQSQRTNWYQILFETYNVPGVTFGIDSLFSFYNYNPSGNKTGLVISCGHEDTNVIPVVDGAGILTDAKRINWGGHQAVDYLNDLMALKYPYFPTKMSYLQYETMYKDYCYVSRNYDEDIEKILTLENLDTNDVVVEAPFTEVLQPQKTEEELRIQAEKRKETGKRLQEQARLKRMEKLVQKQEEFEYFSKVRDQLIDEPKKKVLSVLQNAGFDDERDFKKYLHSLEQSLKKAQMVEAEDDSHLDEMNEDKTAQKFDLLDIADEDLNEDQIKEKRKQRFLKASQDARQKAKEEKERVAKEEEEKKLKEQQWRETDLNGWIKDKRLKLNKLIKRRKEKLKLRDEMKDRKSQVSQNRMKNLASLAEDNVKQGAKRNRHQATIDNDPNDTFGANDEDWLIYTDITQNPEAFEEALEYEYKDIVELERLLLEHDPNFTEEDTLEAQYDWRNSILHLFLRGPRPHDSENIHEQHQMHLNVERIRVPEVIFQPTMGGQDQAGICELSETILLKKFGSQPGKLSQTSIDMVNNVLITGGNAKVPGLKERIVKEFTGFLPTGTNITVNMSSDPSLDAWKGMAALARNEEQYRKTVISKKEYEEYGPEYIKEHKLGNTKYFED D8748B9096B32B53 arp8_yeast Actin-like protein ARP8 ARP8 YOR3348C YOR141C protein protein yeast Saccharomyces cerevisiae Baker's yeast MSQEEAESSIIYEEPIDIPLEDDDDEDELEEENSVPLSSQADQENAENESDDSVDNVVGSETPRSVTGLSVDPRDVADEEDEDEEGEDEDEDEDDNDVDNEDENDNDNANENENELGSSRDKRAPPAVQTSKRYKKYPKLDPAKAPPGKKVPLHLLEKRRLGRIKAAEEFAKTLKKIGIEKVETTTLPATGLFQPLMLINQKNYSSDYLKKDDQIFALRDRKFLRNNNTSQISSTNTPDVIDLKSLPHSEASAAPLNDEIDLNDPTATIVIHPGSNSIKIGFPKDDHPVVVPNCVAVPKKWLDLENSEHVENVCLQREQSEEFNNIKSEMEKNFRERMRYYKRKVPGNAHEQVVSFNENSKPEIISEKNDPSPIEWIFDDSKLYYGSDALRCVDEKFVIRKPFRGGSFNVKSPYYKSLAELISDVTKLLEHALNSETLNVKPTKFNQYKVVLVIPDIFKKSHVETFIRVLLTELQFQAVAIIQESLATCYGAGISTSTCVVNIGAAETRIACVDEGTVLEHSAITLDYGGDDITRLFALFLLQSDFPLQDWKIDSKHGWLLAERLKKNFTTFQDADVAVQLYNFMNRSPNQPTEKYEFKLFDEVMLAPLALFFPQIFKLIRTSSHKNSSLEFQLPESRDLFTNELNDWNSLSQFESKEGNLYCDLNDDLKILNRILDAHNIIDQLQDKPENYGNTLKENFAPLEKAIVQSIANASITADVTRMNSFYSNILIVGGSSKIPALDFILTDRINIWRPSLLSSASFPQFYKKLTKEIKDLEGHYVNAPDKTEDENKQILQAQIKEKIVEELEEQHQNIEHQNGNEHIFPVSIIPPPRDMNPALIIWKGASVLAQIKLVEELFITNSDWDVHGSRILQYKCIFTY 8174851B6B077A19 8174851B6B077A19 h2a_drome Histone H2A His2A H2a His2A:CG31618 CG31618 His2A:CG33808 His2A:CG33814 His2A:CG33817 His2A:CG33820 His2A:CG33823 His2A:CG33826 His2A:CG33829 His2A:CG33832 His2A:CG33835 His2A:CG33838 His2A:CG33841 His2A:CG33844 His2A:CG33847 His2A:CG33850 His2A:CG33862 His2A:CG33865 CG33808 CG33814 CG33817 CG33820 CG33823 CG33826 CG33829 CG33832 CG33835 CG33838 CG33841 CG33844 CG33847 CG33850 CG33862 CG33865 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSGRGKGGKVKGKAKSRSNRAGLQFPVGRIHRLLRKGNYAERVGAGAPVYLAAVMEYLAAEVLELAGNAARDNKKTRIIPRHLQLAIRNDEELNKLLSGVTIAQGGVLPNIQAVLLPKKTEKKA DIP protein P84051 original sequence version: 39 725BA63C393155F6 arp5_human Actin-related protein 5 Sarcoma antigen NY-SAR-16 ACTR5 ARP5 protein protein human Homo sapiens Human MAANVFPFRDARAAPDPVLEAGPVAHGPLPVPLVLDNGSFQVRAGWACPGQDPGPEPRLQFRAVCARGRGGARGASGPQVGNALGSLEPLRWMLRSPFDRNVPVNLELQELLLDYSFQHLGVSSQGCVDHPIVLTEAVCNPLYSRQMMSELLFECYGIPKVAYGIDSLFSFYHNKPKNSMCSGLIISSGYQCTHVLPILEGRLDAKNCKRINLGGSQAAGYLQRLLQLKYPGHLAAITLSRMEEILHEHSYIAEDYVEELHKWRCPDYYENNVHKMQLPFSSKLLGSTLTSEEKQERRQQQLRRLQELNARRREEKLQLDQERLDRLLYVQELLEDGQMDQFHKALIELNMDSPEELQSYIQKLSIAVEQAKQKILQAEVNLEVDVVDSKPETPDLEQLEPSLEDVESMNDFDPLFSEETPGVEKPVTTVQPVFNLAAYHQLFVGTERIRAPEIIFQPSLIGEEQAGIAETLQYILDRYPKDIQEMLVQNVFLTGGNTMYPGMKARMEKELLEMRPFRSSFQVQLASNPVLDAWYGARDWALNHLDDNEVWITRKEYEEKGGEYLKEHCASNIYVPIRLPKQASRSSDAQASSKGSAAGGGGAGEQA DIP protein Q9H9F9 original sequence version: 80 DA3E50AE37BCC363 h4_drome Histone H4 H4 His4r H4r CG3379 His4:CG31611 CG31611 His4 His4:CG33869 His4:CG33871 His4:CG33873 His4:CG33875 His4:CG33877 His4:CG33879 His4:CG33881 His4:CG33883 His4:CG33885 His4:CG33887 His4:CG33889 His4:CG33891 His4:CG33893 His4:CG33895 His4:CG33897 His4:CG33899 His4:CG33901 His4:CG33903 His4:CG33905 His4:CG33907 His4:CG33909 CG33869 CG33871 CG33873 CG33875 CG33877 CG33879 CG33881 CG33883 CG33885 CG33887 CG33889 CG33891 CG33893 CG33895 CG33897 CG33899 CG33901 CG33903 CG33905 CG33907 CG33909 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MTGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG DIP protein P84040 original sequence version: 97 DIP protein P84040 original sequence version: 36 DIP protein P84040 original sequence version: 50 DF3EB26393BD208C ino80-act1-2 6 16 unspecified role unspecified role prey prey 21 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 19 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 13 unspecified role unspecified role prey prey physical association physical association Supp. Fig. 1A, 1B Physical interaction because f down-stream column chromatography - MS is described in M&M. ino80-act1-3 5 23 unspecified role unspecified role neutral component neutral component 22 unspecified role unspecified role neutral component neutral component 20 unspecified role unspecified role neutral component neutral component 19 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component 17 unspecified role unspecified role neutral component neutral component 16 unspecified role unspecified role neutral component neutral component 21 unspecified role unspecified role neutral component neutral component 12 unspecified role unspecified role neutral component neutral component 8 unspecified role unspecified role neutral component neutral component c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 9 unspecified role unspecified role neutral component neutral component 13 unspecified role unspecified role neutral component neutral component 18 unspecified role unspecified role neutral component neutral component 24 unspecified role unspecified role neutral component neutral component physical association physical association Fig. 1, 5A, Supp.Fig. 1 D-I DID-tag on Arp4 protrudes out at the foot of INO80. The DID-tag on Nhp10 protrudes out at the body of INO80. The DID-tag of Ies6 and Ies2 protrude at the neck of INO80. ino80-act1-4 4 16 unspecified role unspecified role neutral component neutral component lys-315 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-328 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-326 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 17 unspecified role unspecified role neutral component neutral component lys-31 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-134 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-31 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-171 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-171 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-295 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-174 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-174 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-66 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-177 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-290 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-454 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-454 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-174 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-31 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-290 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-290 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-432 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-450 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 21 unspecified role unspecified role neutral component neutral component lys-154 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-157 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-181 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-231 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-281 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-414 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-59 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-338 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-157 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-275 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-154 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-183 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-281 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-123 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-157 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-233 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-183 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-198 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-338 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-357 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 24 unspecified role unspecified role neutral component neutral component lys-688 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-688 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-132 sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain lys-176 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-176 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-176 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-176 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-176 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-202 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-211 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-775 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-132 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-132 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-132 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-158 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-158 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-165 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-165 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-165 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-176 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-176 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-396 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-132 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-158 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-165 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-172 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-172 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-176 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-132 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-132 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 12 unspecified role unspecified role neutral component neutral component lys-178 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-137 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-160 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-32 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-87 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-87 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-14 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-74 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-109 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-35 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-192 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 23 unspecified role unspecified role neutral component neutral component lys-731 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-731 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-751 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-467 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-751 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-445 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 13 unspecified role unspecified role neutral component neutral component lys-149 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-161 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-166 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-149 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-166 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-149 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 18 unspecified role unspecified role neutral component neutral component lys-248 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-261 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-267 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-275 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-275 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-286 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-286 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-250 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-275 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-232 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-250 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-261 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-275 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-275 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-286 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-286 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-53 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-295 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 22 unspecified role unspecified role neutral component neutral component lys-16 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-16 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-16 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 9 unspecified role unspecified role neutral component neutral component lys-126 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-54 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-55 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-126 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-80 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-97 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-114 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role neutral component neutral component c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range lys-1082 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-1082 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-370 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-424 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-483 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-794 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-443 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-478 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-456 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-505 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-545 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-1312 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-1350 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-24 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-443 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-427 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-443 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-460 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-108 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-119 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-443 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-119 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-798 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-803 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-1009 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-443 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-483 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-487 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-962 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-1350 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-1082 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-1150 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-1303 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-478 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-460 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-443 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-467 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-427 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-460 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-483 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-443 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-460 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-523 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-798 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-1312 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-427 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-505 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-443 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-427 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-1009 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-1009 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-1009 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-1082 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-443 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role neutral component neutral component lys-210 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-218 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-54 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-473 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-473 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-54 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-201 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-313 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-473 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-473 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-323 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-343 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-473 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-473 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-313 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-323 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-54 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-313 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role neutral component neutral component lys-182 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-182 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-39 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-182 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-182 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-34 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 20 unspecified role unspecified role neutral component neutral component lys-94 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-93 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-89 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-94 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-84 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-93 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-51 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-94 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-94 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-93 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-93 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-93 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-89 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-94 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-94 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-51 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-57 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-89 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-93 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-93 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-94 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-94 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-94 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 19 unspecified role unspecified role neutral component neutral component lys-598 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-609 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-609 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-674 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-690 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-609 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-580 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-609 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain 253 64 65 110 109 66 68 226 248 69 70 100 170 71 171 72 183 73 74 249 227 75 76 93 77 104 78 101 79 106 107 80 172 81 82 247 83 250 84 105 94 85 86 95 192 88 89 189 90 198 91 228 92 230 96 173 174 97 195 98 229 99 102 181 103 199 260 111 112 295 213 113 214 114 206 115 116 215 117 207 118 240 254 119 261 120 235 121 236 122 238 123 239 124 125 204 205 126 127 293 283 128 290 129 259 130 287 131 292 132 288 133 134 289 135 282 294 136 137 237 164 138 186 140 141 305 310 142 306 143 274 144 145 275 146 185 147 309 304 148 276 149 150 187 190 152 153 191 154 193 234 155 156 194 203 157 251 159 160 200 270 161 162 269 163 258 166 219 167 220 168 208 169 209 175 218 241 176 177 221 178 222 179 210 242 180 182 277 267 201 202 268 211 273 212 300 303 216 217 308 223 284 286 224 225 301 263 231 264 232 257 233 243 296 297 244 245 298 299 246 255 279 281 256 262 285 265 291 266 280 272 307 physical association physical association Fig. 2, Supp. Table 2 Only inter-, not intra- xlinks captured. ino80-act1-5 6 11 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey 21 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey 19 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 12 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey physical association physical association Fig.3, Sup. Tables 3 and 4 ino80-act1-6 6 22 unspecified role unspecified role prey prey 19 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 18 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 21 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey physical association physical association Fig. 3B Interaction observed in Arp5-/- cells ino80-act1-9 6 13 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 21 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 19 unspecified role unspecified role prey prey 22 unspecified role unspecified role prey prey 12 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 23 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey physical association physical association Fig.3B Interaction observed in ARP8-/- cells. Taf14 and Actin were present in reduced amounts. ino80-act1-7 6 23 unspecified role unspecified role prey prey 21 unspecified role unspecified role prey prey 17 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 24 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 16 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 18 unspecified role unspecified role prey prey 13 unspecified role unspecified role prey prey physical association physical association Fig.3B Interaction observed in Nhp10-/- cells act1-arp4-19 3 13 unspecified role unspecified role neutral component neutral component lys-166 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-149 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-149 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-161 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-166 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeast Saccharomyces cerevisiae Baker's yeast 16 unspecified role unspecified role neutral component neutral component lys-213 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-213 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeast Saccharomyces cerevisiae Baker's yeast 10 unspecified role unspecified role neutral component neutral component lys-167 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-182 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeast Saccharomyces cerevisiae Baker's yeast 8 unspecified role unspecified role neutral component neutral component lys-198 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-393 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-572 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-962 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-28 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-460 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-487 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range lys-443 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-378 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-1082 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeast Saccharomyces cerevisiae Baker's yeast 24 unspecified role unspecified role neutral component neutral component lys-772 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeast Saccharomyces cerevisiae Baker's yeast 12 unspecified role unspecified role neutral component neutral component lys-85 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeast Saccharomyces cerevisiae Baker's yeast 11 unspecified role unspecified role neutral component neutral component lys-227 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-218 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeast Saccharomyces cerevisiae Baker's yeast 21 unspecified role unspecified role neutral component neutral component lys-298 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-198 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-89 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-357 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-198 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-154 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-233 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-233 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeast Saccharomyces cerevisiae Baker's yeast 20 unspecified role unspecified role neutral component neutral component lys-89 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeast Saccharomyces cerevisiae Baker's yeast 9 unspecified role unspecified role neutral component neutral component lys-55 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-54 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-61 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-54 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeast Saccharomyces cerevisiae Baker's yeast 22 unspecified role unspecified role neutral component neutral component lys-16 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-16 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-26 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeast Saccharomyces cerevisiae Baker's yeast 19 unspecified role unspecified role neutral component neutral component lys-674 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeast Saccharomyces cerevisiae Baker's yeast 23 unspecified role unspecified role neutral component neutral component lys-489 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-315 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeast Saccharomyces cerevisiae Baker's yeast 18 unspecified role unspecified role neutral component neutral component lys-275 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-261 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-261 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-232 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-275 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-232 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeast Saccharomyces cerevisiae Baker's yeast 17 unspecified role unspecified role neutral component neutral component lys-290 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-31 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-290 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-66 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain yeast Saccharomyces cerevisiae Baker's yeast 14 unspecified role unspecified role neutral component neutral component lys-28 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-15 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-24 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-15 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-19 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-15 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-15 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-65 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-10 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-28 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-15 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-5 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 27 unspecified role unspecified role neutral component neutral component lys-13 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-6 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-9 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-9 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-32 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-45 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-9 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-60 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-45 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-6 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 15 unspecified role unspecified role neutral component neutral component lys-18 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-15 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-15 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-18 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-15 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-18 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-118 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-106 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-8 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-106 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-118 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-118 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-55 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-118 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-18 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-11 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-12 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-12 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-12 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 25 unspecified role unspecified role neutral component neutral component lys-75 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-75 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-13 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-15 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-11 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-9 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-9 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-122 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-122 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-122 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain lys-6 cross linker cross linker crosslinker crosslinker certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 495 373 374 480 483 375 376 481 482 377 379 471 488 380 382 461 383 473 385 456 457 386 387 485 486 388 389 466 390 467 391 468 393 479 476 394 395 451 397 449 458 399 401 445 446 402 404 443 441 405 406 455 407 462 477 408 493 409 494 410 411 452 413 454 415 444 459 416 417 475 450 418 420 489 421 474 422 491 447 424 472 426 427 470 460 429 430 490 431 465 448 432 478 433 463 434 442 436 487 437 492 438 469 439 physical association physical association Supp. Fig.5, Supp. Table 6 nhp10-ies3-2 7 8 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role prey prey 12 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 22 unspecified role unspecified role prey prey association association Supp. fig. 2E nhp10-ies1-2 1 19 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role bait bait region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. Fig. 2F, 4C act1-arp4-18 2 20 unspecified role unspecified role unspecified role unspecified role region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role unspecified role unspecified role hsa region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role unspecified role unspecified role 24 unspecified role unspecified role unspecified role unspecified role 16 unspecified role unspecified role unspecified role unspecified role association association Supp Fig 2G, 4C actr5-ies6 2 26 unspecified role unspecified role unspecified role unspecified role 9 unspecified role unspecified role unspecified role unspecified role region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Supp. fig. 2H, 4C