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  <entry>
    <source releaseDate="2022-02-03">
      <names>
        <shortLabel>MINT</shortLabel>
        <fullName>MINT, Dpt of Biology, University of Rome Tor Vergata</fullName>
        <alias type="synonym" typeAc="MI:1041">MINT</alias>
      </names>
      <bibref>
        <xref>
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        </xref>
      </bibref>
      <xref>
        <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0471" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="mint" dbAc="MI:0471" id="MINT-80017" refType="identity" refTypeAc="MI:0356"/>
        <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1579228" refType="identity" refTypeAc="MI:0356"/>
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        <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
      </xref>
      <attributeList>
        <attribute name="url" nameAc="MI:0614">http://mint.bio.uniroma2.it/mint</attribute>
        <attribute name="url" nameAc="MI:0614">url&amp;gt;http://mint.bio.uniroma2.it/mint/</attribute>
        <attribute name="url" nameAc="MI:0614">https://mint.bio.uniroma2.it</attribute>
        <attribute name="id-validation-regexp" nameAc="MI:0628">MINT-[0-9]+</attribute>
        <attribute name="search-url" nameAc="MI:0615">https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac}</attribute>
        <attribute name="definition">The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules.</attribute>
        <attribute name="url" nameAc="MI:0614">http://mint.bio.uniroma2.it</attribute>
      </attributeList>
    </source>
    <experimentList>
      <experimentDescription id="1">
        <names>
          <fullName>Potassium ion-dependent trehalose phosphorylase from halophilic&#xd;
Bacillus selenitireducens MLS10</fullName>
        </names>
        <bibref>
          <xref>
            <primaryRef db="pubmed" dbAc="MI:0446" id="24021648" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-8697764" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="imex" dbAc="MI:0670" id="IM-21409" refType="imex-primary" refTypeAc="MI:0662"/>
          </xref>
          <attributeList>
            <attribute name="publication title" nameAc="MI:1091">Potassium ion-dependent trehalose phosphorylase from halophilic&#xd;
Bacillus selenitireducens MLS10</attribute>
            <attribute name="journal" nameAc="MI:0885">FEBS Lett. (0014-5793)</attribute>
            <attribute name="publication year" nameAc="MI:0886">2013</attribute>
            <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
            <attribute name="author-list" nameAc="MI:0636">Nihira T., Saito Y., Chiku K., Kitaoka M., Ohtsubo K., Nakai H.</attribute>
            <attribute name="contact-email" nameAc="MI:0634">nakai@agr.niigata-u.ac.jp</attribute>
            <attribute name="author-announcement">30-Sept-2013: Contacted by IntAct-Help.</attribute>
            <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
            <attribute name="imex curation" nameAc="MI:0959"/>
          </attributeList>
        </bibref>
        <xref>
          <primaryRef db="pubmed" dbAc="MI:0446" id="24021648" refType="primary-reference" refTypeAc="MI:0358"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-21409" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <hostOrganismList>
          <hostOrganism ncbiTaxId="-1">
            <names>
              <shortLabel>in vitro</shortLabel>
              <fullName>In vitro</fullName>
            </names>
          </hostOrganism>
        </hostOrganismList>
        <interactionDetectionMethod>
          <names>
            <shortLabel>molecular sieving</shortLabel>
            <fullName>molecular sieving</fullName>
            <alias type="go synonym" typeAc="MI:0303">Gel Filtration</alias>
            <alias type="go synonym" typeAc="MI:0303">Size Exclusion Chromatography</alias>
            <alias type="synonym" typeAc="MI:1041">Size Exclusion Chromatography</alias>
            <alias type="synonym" typeAc="MI:1041">Gel Filtration</alias>
            <alias type="synonym" typeAc="MI:1041">Sizing column</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0071" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1175" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="7708014" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionDetectionMethod>
        <participantIdentificationMethod>
          <names>
            <shortLabel>predetermined</shortLabel>
            <fullName>predetermined participant</fullName>
            <alias type="synonym" typeAc="MI:1041">predetermined</alias>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0396" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1465" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </participantIdentificationMethod>
        <attributeList>
          <attribute name="author-list" nameAc="MI:0636">Nihira T., Saito Y., Chiku K., Kitaoka M., Ohtsubo K., Nakai H.</attribute>
          <attribute name="contact-email" nameAc="MI:0634">nakai@agr.niigata-u.ac.jp</attribute>
          <attribute name="accepted">Accepted 2013-AUG-28 by LPERFETTO</attribute>
          <attribute name="author-announcement">30-Sept-2013: Contacted by IntAct-Help.</attribute>
          <attribute name="journal" nameAc="MI:0885">FEBS Lett. (0014-5793)</attribute>
          <attribute name="publication year" nameAc="MI:0886">2013</attribute>
          <attribute name="full coverage" nameAc="MI:0957">Only protein-protein interactions</attribute>
          <attribute name="imex curation" nameAc="MI:0959"/>
          <attribute name="curation depth" nameAc="MI:0955">imex curation</attribute>
        </attributeList>
      </experimentDescription>
    </experimentList>
    <interactorList>
      <interactor id="2">
        <names>
          <shortLabel>d6xsd4_bacie</shortLabel>
          <alias type="locus name" typeAc="MI:0305">Bsel_1207</alias>
        </names>
        <xref>
          <primaryRef db="uniprotkb" dbAc="MI:0486" id="D6XSD4" version="TrEMBL_15" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="ensemblbacteria" id="ADH98720" version="TrEMBL_56" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="intact" dbAc="MI:0469" id="EBI-8697787" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="refseq" dbAc="MI:0481" id="WP_013172144.1"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR012341"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR008928"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR011013"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR005194"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR005195"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR005196"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR017045"/>
          <secondaryRef db="ensemblbacteria" id="ADH98720" version="TrEMBL_56" refType="transcript"/>
          <secondaryRef db="ensemblbacteria" id="Bsel_1207" version="TrEMBL_56" refType="gene"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0005975" version="TrEMBL_56"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0016787" version="TrEMBL_56"/>
          <secondaryRef db="go" dbAc="MI:0448" id="GO:0030246" version="TrEMBL_56"/>
          <secondaryRef db="interpro" dbAc="MI:0449" id="IPR037018"/>
        </xref>
        <interactorType>
          <names>
            <shortLabel>protein</shortLabel>
            <fullName>protein</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0326" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-619654" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
            <secondaryRef db="so" dbAc="MI:0601" id="SO:0000358" refType="see-also" refTypeAc="MI:0361"/>
          </xref>
        </interactorType>
        <organism ncbiTaxId="439292">
          <names>
            <shortLabel>bacie</shortLabel>
            <fullName>Bacillus selenitireducens (strain ATCC 700615 / DSM 15326 / MLS10)</fullName>
          </names>
        </organism>
        <sequence>MSWHISSKALDQDQLLVDESLFAQGNGYLGIRGNFEEGYAADMTTIRGAYLNAFHDVVPIEYGEKLFGFPDTQQKLVNNIDVQSIRIIIDGDDFSLFQGEILDFERFLYMKDGYSERRIHWKSPAGKEVSIHFYRMISFTDKELFVQWIDIEPISQVQEIEIVSTVDGDVSNFVEASDPRVASGHAKRLHVTDVQDKGPYMQVSDETEVSKLKVSATTWVSASREALKESRSSFEGKVEERRTFSGEGSLRYEKRSVYTDTYRHESSPSEAGFELAKRLSAVSLDELIRGQRAYLNRFWQTSDIRIAGDDKLQEGIRFNLYQLLQSVGKEPTSNISAKGLSGEGYEGHYFWDTEIYMYPPFLMTDPEIAKNLLLHRFSLLDAARERAKEMGHRQGALFPWRTISGGECSAFFPAGSAQYHISADIAYSFIQYYLTTGDEVFLADYGAELLVETARLWIDTGHMKDGRFRIDDVTGPDEYTCIVNNNYYTNSMAKHNLEWAVKAMALVEERHPTVYKNLAARLAVTDDELKAFREASESMYYPYSDSHRIHAQDDTFLNKQVWDIEGTPKEEFPLLLHYHPLTLYRYQVCKQADTVLSHFLLEDEADEETIRRSYDYYEQVTTHDSSLSFCVFSIMAAKLGYEKKAYEYFSETARLDLDNTHKNTKDGLHMANMGGTWLALVSGFGGMRVKETGLHFAPRLPEAWDSLSFQIQYQGRVIALDIGRDKTVYTLTEGDVINMYHHGKLFTLTEQQTLLHA</sequence>
        <attributeList>
          <attribute name="crc64">AE37D8D367AA3B88</attribute>
        </attributeList>
      </interactor>
    </interactorList>
    <interactionList>
      <interaction id="3" imexId="IM-21409-1">
        <names>
          <shortLabel>d6xsd4</shortLabel>
        </names>
        <xref>
          <primaryRef db="intact" dbAc="MI:0469" id="EBI-8697783" refType="identity" refTypeAc="MI:0356"/>
          <secondaryRef db="imex" dbAc="MI:0670" id="IM-21409-1" refType="imex-primary" refTypeAc="MI:0662"/>
        </xref>
        <experimentList>
          <experimentRef>1</experimentRef>
        </experimentList>
        <participantList>
          <participant id="4">
            <names>
              <alias type="author assigned name" typeAc="MI:0345">Bsel_1207</alias>
            </names>
            <interactorRef>2</interactorRef>
            <biologicalRole>
              <names>
                <shortLabel>unspecified role</shortLabel>
                <fullName>unspecified role</fullName>
              </names>
              <xref>
                <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="intact" dbAc="MI:0469" id="EBI-77781" refType="identity" refTypeAc="MI:0356"/>
                <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
              </xref>
            </biologicalRole>
            <experimentalRoleList>
              <experimentalRole>
                <names>
                  <shortLabel>unspecified role</shortLabel>
                  <fullName>unspecified role</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0499" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1383079" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalRole>
            </experimentalRoleList>
            <experimentalPreparationList>
              <experimentalPreparation>
                <names>
                  <shortLabel>purified</shortLabel>
                  <fullName>purified</fullName>
                </names>
                <xref>
                  <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0350" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="intact" dbAc="MI:0469" id="EBI-1537811" refType="identity" refTypeAc="MI:0356"/>
                  <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
                </xref>
              </experimentalPreparation>
            </experimentalPreparationList>
            <hostOrganismList>
              <hostOrganism ncbiTaxId="83333">
                <names>
                  <shortLabel>ecoli</shortLabel>
                  <fullName>Escherichia coli (strain K12)</fullName>
                </names>
              </hostOrganism>
            </hostOrganismList>
            <stoichiometry value="2"/>
          </participant>
        </participantList>
        <interactionType>
          <names>
            <shortLabel>direct interaction</shortLabel>
            <fullName>direct interaction</fullName>
          </names>
          <xref>
            <primaryRef db="psi-mi" dbAc="MI:0488" id="MI:0407" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="intact" dbAc="MI:0469" id="EBI-608833" refType="identity" refTypeAc="MI:0356"/>
            <secondaryRef db="pubmed" dbAc="MI:0446" id="14755292" refType="primary-reference" refTypeAc="MI:0358"/>
          </xref>
        </interactionType>
        <attributeList>
          <attribute name="figure legend" nameAc="MI:0599">f3</attribute>
        </attributeList>
      </interaction>
    </interactionList>
  </entry>
</entrySet>