IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Characterizing affinity epitopes between prion protein and β-amyloid using an epitope mapping immunoassay. Characterizing affinity epitopes between prion protein and β-amyloid using an epitope mapping immunoassay. Exp. Mol. Med. (1226-3613) 2013 imex curation Kang M., Kim S.Y., An S.S., Ju Y.R. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease Alzheimers - Interactions investigated in the context of Alzheimers disease seong.an@gmail.com 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa predetermined predetermined participant predetermined Kang M., Kim S.Y., An S.S., Ju Y.R. Parkinsons - Interactions investigated in the context of Parkinsons disease A sulfhydryl group from a cysteine at the end of each synthetic peptide was conjugated through a maleimide reaction onto a maleimide-activated plate. seong.an@gmail.com Accepted 2013-NOV-28 by ORCHARD Exp. Mol. Med. (1226-3613) 2013 Only protein-protein interactions imex curation there are dimers, trimers and tetramers shown (nothing more) - so have entered all.(M. Wt of monomer is 4.5 kDa and this fits with the values shown on Fig 4A) Characterizing affinity epitopes between prion protein and β-amyloid using an epitope mapping immunoassay. Characterizing affinity epitopes between prion protein and β-amyloid using an epitope mapping immunoassay. Exp. Mol. Med. (1226-3613) 2013 imex curation Kang M., Kim S.Y., An S.S., Ju Y.R. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease Alzheimers - Interactions investigated in the context of Alzheimers disease seong.an@gmail.com 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro electrophoresis electrophoretic mobility-based method electrophoresis weight silver stain molecular weight estimation by silver staining weight silver stain Kang M., Kim S.Y., An S.S., Ju Y.R. Parkinsons - Interactions investigated in the context of Parkinsons disease seong.an@gmail.com Accepted 2013-NOV-28 by ORCHARD Exp. Mol. Med. (1226-3613) 2013 Only protein-protein interactions imex curation there are dimers, trimers and tetramers shown (nothing more) - so have entered all.(M. Wt of monomer is 4.5 kDa and this fits with the values shown on Fig 4A) Characterizing affinity epitopes between prion protein and β-amyloid using an epitope mapping immunoassay. Characterizing affinity epitopes between prion protein and β-amyloid using an epitope mapping immunoassay. Exp. Mol. Med. (1226-3613) 2013 imex curation Kang M., Kim S.Y., An S.S., Ju Y.R. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease Alzheimers - Interactions investigated in the context of Alzheimers disease seong.an@gmail.com 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down antibody detection identification by antibody antibody detection Kang M., Kim S.Y., An S.S., Ju Y.R. Parkinsons - Interactions investigated in the context of Parkinsons disease PrPC was added to Ab42- -conjugated magnetic beads. seong.an@gmail.com Accepted 2013-NOV-28 by ORCHARD Exp. Mol. Med. (1226-3613) 2013 Only protein-protein interactions imex curation there are dimers, trimers and tetramers shown (nothing more) - so have entered all.(M. Wt of monomer is 4.5 kDa and this fits with the values shown on Fig 4A) prio_human Major prion protein PrP27-30 PrP33-35C ASCR PRNP PRIP PRP ALTPRP protein protein human Homo sapiens Human MANLGCWMLVLFVATWSDLGLCKKRPKPGGWNTGGSRYPGQGSPGGNRYPPQGGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQPHGGGWGQGGGTHSQWNKPSKPKTNMKHMAGAAAAGAVVGGLGGYMLGSAMSRPIIHFGSDYEDRYYRENMHRYPNQVYYRPMDEYSNQNNFVHDCVNITIKQHTVTTTTKGENFTETDVKMMERVVEQMCITQYERESQAYYQRGSSMVLFSSPPVILLISFLIFLIVG DIP protein P04156 original sequence version: 125 DIP protein P04156 original sequence version: 122 DIP protein P04156 original sequence version: 147 DIP protein P04156 original sequence version: 141 DIP protein P04156 original sequence version: 166 DIP protein P04156-1 original sequence version: 166 43DB596BAAA66484 p05067-pro_0000000093 Amyloid-beta protein 40 APP A4 AD1 ABPP Alzheimer disease amyloid protein Cerebral vascular amyloid peptide PreA4 Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVV 672 711 D563D7858C3768F1 p05067-pro_0000000092 Amyloid-beta protein 42 Alzheimer disease amyloid protein Cerebral vascular amyloid peptide APP A4 AD1 ABPP PreA4 Protease nexin-II APPI Amyloid precursor protein Amyloid-beta A4 protein Alzheimer disease amyloid A4 protein homolog Amyloid-beta (A4) precursor protein protein protein human Homo sapiens Human DAEFRHDSGYEVHHQKLVFFAEDVGSNKGAIIGLMVGGVVIA 672 713 3AC85563D7858C37 prnp-app-4 1 6 unspecified role unspecified role prey prey chemical synthesis Chemical synthesis (Chemical synthesis) 4 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Table 1 prnp-app-3 1 4 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 5 unspecified role unspecified role prey prey chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Table 1i Monomeric ab40 bound to PrPC app-prnp-2 3 4 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role bait bait chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Fig 5 app-1 2 6 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Fig 4A app-1 2 6 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Fig 4A app-1 2 6 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Fig 4A