MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Structural basis for recognition of the third SH3 domain of full-length R85 (R85FL)/ponsin by ataxin-7. Structural basis for recognition of the third SH3 domain of full-length R85 (R85FL)/ponsin by ataxin-7. FEBS Lett. (0014-5793) 2013 imex curation Jiang Y.J., Zhou C.J., Zhou Z.R., Wu M., Hu H.Y. Only protein-protein interactions human Homo sapiens Human fluorescence imaging fluorescence microscopy fluorescence imaging experimental particp experimental participant identification experimental particp Jiang Y.J., Zhou C.J., Zhou Z.R., Wu M., Hu H.Y. FEBS Lett. (0014-5793) 2013 imex curation Structural basis for recognition of the third SH3 domain of full-length R85 (R85FL)/ponsin by ataxin-7. Structural basis for recognition of the third SH3 domain of full-length R85 (R85FL)/ponsin by ataxin-7. FEBS Lett. (0014-5793) 2013 imex curation Jiang Y.J., Zhou C.J., Zhou Z.R., Wu M., Hu H.Y. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Jiang Y.J., Zhou C.J., Zhou Z.R., Wu M., Hu H.Y. FEBS Lett. (0014-5793) 2013 imex curation Structural basis for recognition of the third SH3 domain of full-length R85 (R85FL)/ponsin by ataxin-7. Structural basis for recognition of the third SH3 domain of full-length R85 (R85FL)/ponsin by ataxin-7. FEBS Lett. (0014-5793) 2013 imex curation Jiang Y.J., Zhou C.J., Zhou Z.R., Wu M., Hu H.Y. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Jiang Y.J., Zhou C.J., Zhou Z.R., Wu M., Hu H.Y. FEBS Lett. (0014-5793) 2013 imex curation Structural basis for recognition of the third SH3 domain of full-length R85 (R85FL)/ponsin by ataxin-7. Structural basis for recognition of the third SH3 domain of full-length R85 (R85FL)/ponsin by ataxin-7. FEBS Lett. (0014-5793) 2013 imex curation Jiang Y.J., Zhou C.J., Zhou Z.R., Wu M., Hu H.Y. Only protein-protein interactions in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Jiang Y.J., Zhou C.J., Zhou Z.R., Wu M., Hu H.Y. FEBS Lett. (0014-5793) 2013 imex curation srbs1_human Sorbin and SH3 domain-containing protein 1 KIAA0894 Ponsin c-Cbl-associated protein SH3 domain protein 5 SH3P12 SORBS1 KIAA1296 SH3D5 protein protein human Homo sapiens Human MSSECDGGSKAVMNGLAPGSNGQDKATADPLRARSISAVKIIPVKTVKNASGLVLPTDMDLTKICTGKGAVTLRASSSYRETPSSSPASPQETRQHESKPGLEPEPSSADEWRLSSSADANGNAQPSSLAAKGYRSVHPNLPSDKSQDATSSSAAQPEVIVVPLYLVNTDRGQEGTARPPTPLGPLGCVPTIPATASAASPLTFPTLDDFIPPHLQRWPHHSQPARASGSFAPISQTPPSFSPPPPLVPPAPEDLRRVSEPDLTGAVSSTDSSPLLNEVSSSLIGTDSQAFPSVSKPSSAYPSTTIVNPTIVLLQHNREQQKRLSSLSDPVSERRVGEQDSAPTQEKPTSPGKAIEKRAKDDSRRVVKSTQDLSDVSMDEVGIPLRNTERSKDWYKTMFKQIHKLNRDTPEENPYFPTYKFPELPEIQQTSEEDNPYTPTYQFPASTPSPKSEDDDSDLYSPRYSFSEDTKSPLSVPRSKSEMSYIDGEKVVKRSATLPLPARSSSLKSSSERNDWEPPDKKVDTRKYRAEPKSIYEYQPGKSSVLTNEKMSRDISPEEIDLKNEPWYKFFSELEFGKPPPKKIWDYTPGDCSILPREDRKTNLDKDLSLCQTELEADLEKMETLNKAPSANVPQSSAISPTPEISSETPGYIYSSNFHAVKRESDGAPGDLTSLENERQIYKSVLEGGDIPLQGLSGLKRPSSSASTKDSESPRHFIPADYLESTEEFIRRRHDDKEKLLADQRRLKREQEEADIAARRHTGVIPTHHQFITNERFGDLLNIDDTAKRKSGSEMRPARAKFDFKAQTLKELPLQKGDIVYIYKQIDQNWYEGEHHGRVGIFPRTYIELLPPAEKAQPKKLTPVQVLEYGEAIAKFNFNGDTQVEMSFRKGERITLLRQVDENWYEGRIPGTSRQGIFPITYVDVIKRPLVKNPVDYMDLPFSSSPSRSATASPQFSSHSKLITPAPSSLPHSRRALSPEMHAVTSEWISLTVGVPGRRSLALTPPLPPLPEASIYNTDHLALSPRASPSLSLSLPHLSWSDRPTPRSVASPLALPSPHKTYSLAPTSQASLHMNGDGGVHTPSSGIHQDSFLQLPLGSSDSVISQLSDAFSSQSKRQPWREESGQYERKAERGAGERGPGGPKISKKSCLKPSDVVRCLSTEQRLSDLNTPEESRPGKPLGSAFPGSEAEQTERHRGGEQAGRKAARRGGSQQPQAQQRRVTPDRSQTSQDLFSYQALYSYIPQNDDELELRDGDIVDVMEKCDDGWFVGTSRRTKQFGTFPGNYVKPLYL 70DA4169B6D82F06 atx7_human Ataxin-7 Spinocerebellar ataxia type 7 protein ATXN7 SCA7 protein protein human Homo sapiens Human MSERAADDVRGEPRRAAAAAGGAAAAAARQQQQQQQQQQPPPPQPQRQQHPPPPPRRTRPEDGGPGAASTSAAAMATVGERRPLPSPEVMLGQSWNLWVEASKLPGKDGTELDESFKEFGKNREVMGLCREDMPIFGFCPAHDDFYLVVCNDCNQVVKPQAFQSHYERRHSSSSKPPLAVPPTSVFSFFPSLSKSKGGSASGSNRSSSGGVLSASSSSSKLLKSPKEKLQLRGNTRPMHPIQQSRVPHGRIMTPSVKVEKIHPKMDGTLLKSAVGPTCPATVSSLVKPGLNCPSIPKPTLPSPGQILNGKGLPAPPTLEKKPEDNSNNRKFLNKRLSEREFDPDIHCGVIDLDTKKPCTRSLTCKTHSLTQRRAVQGRRKRFDVLLAEHKNKTREKELIRHPDSQQPPQPLRDPHPAPPRTSQEPHQNPHGVIPSESKPFVASKPKPHTPSLPRPPGCPAQQGGSAPIDPPPVHESPHPPLPATEPASRLSSEEGEGDDKEESVEKLDCHYSGHHPQPASFCTFGSRQIGRGYYVFDSRWNRLRCALNLMVEKHLNAQLWKKIPPVPSTTSPISTRIPHRTNSVPTSQCGVSYLAAATVSTSPVLLSSTCISPNSKSVPAHGTTLNAQPAASGAMDPVCSMQSRQVSSSSSSPSTPSGLSSVPSSPMSRKPQKLKSSKSLRPKESSGNSTNCQNASSSTSGGSGKKRKNSSPLLVHSSSSSSSSSSSSHSMESFRKNCVAHSGPPYPSTVTSSHSIGLNCVTNKANAVNVRHDQSGRGPPTGSPAESIKRMSVMVNSSDSTLSLGPFIHQSNELPVNSHGSFSHSHTPLDKLIGKKRKCSPSSSSINNSSSKPTKVAKVPAVNNVHMKHTGTIPGAQGLMNSSLLHQPKARP 9AEA787D77103C5F atxn7-sorbs1 3 Atx7 6 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined SH3C 5 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey purified purified flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction homomint domino mint f1a f1d f1e The results show that Atx7 PRR can interact with the third SH3 domain (as referred to SH3C) (Fig. 1D), but it neither binds with the first domain (SH3A) (Fig. 1B) nor the second (SH3B) (Fig. 1C). SH3 domain canonically binds with such amino-acid sequence as R/KxxPxxP (class I) or PxxPxR/K (class II) [10,12]. So we dissected the Atx7 PRR sequence into two parts, namely Atx7_PP1 and Atx7_PP2 (Fig. 1A), each containing a class-II consensus peptide motif. Intriguingly, SH3C only interacts with Atx7_PP2 but not with Atx7_PP1 (Fig. 1E). atxn7-sorbs1-1 4 Atx7 6 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain SH3C 5 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction homomint domino mint The atomic coordinates and structure factors of free and PP2-bound SHC3 have been deposited in the PDB under codes 2LJ0 and 2LJ1, respectively. st1 f2c f3b f3c f3d f4a f4b sf2a sf2f atxn7-sorbs1-2 2 Atx7 PP2 6 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain SH3C 5 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction homomint domino mint t1 f3a sf1b atxn7-sorbs1-3 2 Atx7 PP12 6 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain SH3C 5 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction homomint domino mint t1 sf1a atxn7-sorbs1-4 2 SH3C 5 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Atx7 PP2 6 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified O15265:p.Arg56Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Tue Jul 12 10:16:34 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 18:55:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg56ala' Tue Jun 30 16:42:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg56Ala' binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction t1 homomint domino mint atxn7-sorbs1-5 2 SH3C 5 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Atx7 PP2 6 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified O15265:p.Arg57Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Tue Jul 12 10:16:30 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 18:15:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg57ala' Tue Jun 30 16:18:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg57Ala' binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction t1 homomint domino mint atxn7-sorbs1-6 2 SH3C 5 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Atx7 PP2 6 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain O15265:p.Thr58Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T A Tue Jul 12 10:54:47 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 18:02:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr58ala' Tue Jun 30 19:14:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr58Ala' direct interaction direct interaction t1 homomint domino mint atxn7-sorbs1-7 2 Atx7 PP2 6 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain O15265:p.Arg59Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Tue Jul 12 10:55:03 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 19:46:08 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg59ala' Tue Jun 30 15:34:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg59Ala' SH3C 5 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction t1 homomint domino mint atxn7-sorbs1-8 2 Atx7 PP2 6 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain SH3C 5 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified Q9BX66:p.Tyr1240Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain Y A Tue Jul 12 10:55:56 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 16:09:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1240ala' Tue Jun 30 17:55:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr1240Ala' binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction t1 homomint domino mint atxn7-sorbs1-9 2 SH3C 5 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q9BX66:p.Asp1247Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain D A Tue Jul 12 10:19:00 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 19:14:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp1247ala' Tue Jun 30 17:16:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp1247Ala' Atx7 PP2 6 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction t1 homomint domino mint atxn7-sorbs1-10 2 SH3C 5 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q9BX66:p.Asp1248Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain D A Tue Jul 12 10:19:10 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 20:22:05 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp1248ala' Tue Jun 30 16:48:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp1248Ala' Atx7 PP2 6 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction t1 homomint domino mint atxn7-sorbs1-11 2 Atx7 PP2 6 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain SH3C 5 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified Q9BX66:p.Asp1266Ala mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain D A Tue Jul 12 10:19:17 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 17:09:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp1266ala' Tue Jun 30 19:07:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp1266Ala' binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction t1 homomint domino mint atxn7-sorbs1-12 2 Atx7 PP2 6 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain SH3C 5 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q9BX66:p.Tyr1286Ala mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain Y A Tue Jul 12 10:11:14 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 17:29:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1286ala' Tue Jun 30 18:10:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr1286Ala' direct interaction direct interaction t1 homomint domino mint atxn7-sorbs1-13 2 SH3C 5 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q9BX66:p.Thr1281Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain T A Tue Jul 12 10:51:49 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 16:58:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr1281ala' Tue Jun 30 17:55:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr1281Ala' Atx7 PP2 6 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction t1 homomint domino mint atxn7-sorbs1-14 1 Atx7100Q 6 tag fluorescence tag visualisation by fluorescence tag fluorescence experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined SH3C 5 anti tag immunost anti tag immunostaining anti tag immunost experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain flag tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q9BX66:p.Asp1266Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain D A Tue Jul 12 10:51:57 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 18:47:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp1266ala' Tue Jun 30 16:51:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp1266Ala' colocalization colocalization f5 homomint domino mint Normal Atx7 (Atx710Q) is a nucleus localized protein, while R85FL is distributed mainly in cytosol (Fig. 5, 1st row). The polyQ expanded Atx7 (Atx7100Q) forms nuclear inclusion bodies, which can sequester R85FL into the nucleus (Fig. 5, 2nd row). As a control, double mutation (I16A/Q18A) in the SH3C moiety of R85FL, which does not alter the interaction with Atx7_PP2, can also be sequestered into the nucleus by Atx7100Q (Fig. 5, 3rd row). However, the D39A mutant in the SH3C moiety of R85FL that significantly reduces the binding affinity to the PP2 motif of Atx7 cannot be sequestered by Atx7100Q (Fig. 5, 4th row). Moreover, deletion of SH3C in R85FL significantly abolishes sequestration of R85FL into the nucleus by Atx7100Q (Fig. 5, 5th row).