IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ A bacterial virulence protein promotes pathogenicity by inhibiting the bacterium's own F1Fo ATP synthase. A bacterial virulence protein promotes pathogenicity by inhibiting the bacterium's own F1Fo ATP synthase. Cell (0092-8674) 2013 imex curation Lee E.J., Pontes M.H., Groisman E.A. Proteins were synthesized using cell-free PURExpress in vitro protein synthesis system in the presence of 0.12 mg/ml proteoliposomes for 2 hr at 37C eduardo.groisman@yale.edu 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Lee E.J., Pontes M.H., Groisman E.A. Proteins were synthesized using cell-free PURExpress in vitro protein synthesis system in the presence of 0.12 mg/ml proteoliposomes for 2 hr at 37C eduardo.groisman@yale.edu Accepted 2013-DEC-03 by PPORRAS Cell (0092-8674) 2013 Only protein-protein interactions imex curation A bacterial virulence protein promotes pathogenicity by inhibiting the bacterium's own F1Fo ATP synthase. A bacterial virulence protein promotes pathogenicity by inhibiting the bacterium's own F1Fo ATP synthase. Cell (0092-8674) 2013 imex curation Lee E.J., Pontes M.H., Groisman E.A. Proteins were synthesized using cell-free PURExpress in vitro protein synthesis system in the presence of 0.12 mg/ml proteoliposomes for 2 hr at 37C eduardo.groisman@yale.edu 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions salt1 Salmonella typhimurium (strain 14028s / SGSC 2262) crosslink cross-linking study crosslink ms participant Identification by mass spectrometry ms participant Lee E.J., Pontes M.H., Groisman E.A. Proteins were synthesized using cell-free PURExpress in vitro protein synthesis system in the presence of 0.12 mg/ml proteoliposomes for 2 hr at 37C eduardo.groisman@yale.edu Accepted 2013-DEC-03 by PPORRAS Cell (0092-8674) 2013 Only protein-protein interactions imex curation A bacterial virulence protein promotes pathogenicity by inhibiting the bacterium's own F1Fo ATP synthase. A bacterial virulence protein promotes pathogenicity by inhibiting the bacterium's own F1Fo ATP synthase. Cell (0092-8674) 2013 imex curation Lee E.J., Pontes M.H., Groisman E.A. Proteins were synthesized using cell-free PURExpress in vitro protein synthesis system in the presence of 0.12 mg/ml proteoliposomes for 2 hr at 37C eduardo.groisman@yale.edu 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions salt1 Salmonella typhimurium (strain 14028s / SGSC 2262) anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Lee E.J., Pontes M.H., Groisman E.A. Proteins were synthesized using cell-free PURExpress in vitro protein synthesis system in the presence of 0.12 mg/ml proteoliposomes for 2 hr at 37C eduardo.groisman@yale.edu Accepted 2013-DEC-03 by PPORRAS Cell (0092-8674) 2013 Only protein-protein interactions imex curation a0a0f6bam8_salt1 Protein HflC hflC STM14_5245 protein protein salt1 Salmonella typhimurium (strain 14028s / SGSC 2262) MRKSVIAIIIIVLVVLYMSVFVVKEGERGITLRFGKVLRDDENKPLVYAPGLHFKIPFIESVKMLDARIQTMDNQADRFVTKEKKDLIVDSYIKWRISDFSRYYLATGGGDISQAEVLLKRKFSDRLRSEIGRLDVKDIVTDSRGRLTLEVRDALNSGSAGTDDEVATPAADDAIAEAAERVTAETKGKVPVINPNSMAALGIEVVDVRIKQINLPTEVSEAIYNRMRAEREAVARRHRSQGQEEAEKLRAAADYEVTKTLAEAERQGRIMRGEGDAEAAKLFADAFSQDPDFYAFIRSLRAYEKSFEGNQDVMVLSPDSDFFRYMKTPSSTTR 4C9A5456863C6CEF a0a0f6bam7_salt1 Protein HflK hflK STM14_5244 protein protein salt1 Salmonella typhimurium (strain 14028s / SGSC 2262) MAWNQPGNNGQDRDPWGSSKPGSNSGGNGNKGGRDQGPPDLDDIFRKLSKKLGGFGGGKGTGSGGGSSSQGPRPQLGGRIVAIAAAAVVIIWAASGFYTIKEAERGVVTRFGKFSHLVEPGLNWKPTFIDDVTPVNVEAVRELAASGVMLTSDENVVRVEMNVQYRVTDPQKYLFSVTSPDDSLRQATDSALRGVIGKYTMDRILTEGRTVIRSDTQRELEETIKPYNMGITLLDVNFQAARPPEEVKAAFDDAIAARENEQQYIREAEAYTNEVQPRANGQAQRILEEARAYKTQTILEAQGEVARFAKILPEYKAAPQITRERLYIETMEKVLSHTRKVLVNDKSGNLMVLPLDQMLKGGNAPAAKSDNSASNLLRLPPSTKSNASGASNTSSSNQGDIMDQRRANAQRNDYQRQGE 7263D54F23B0DA95 mgtc_salt1 Protein MgtC mgtC STM14_4538 protein protein salt1 Salmonella typhimurium (strain 14028s / SGSC 2262) MEERMLMFPYILNLLAAMLLGALIGAERQWRQRMAGLRTNALVATGAAVFILSSMTTSPDSPGRIAAQIVSGIGFLGAGVIMREGMNVRGLNTAATLWCSAGIGVLCGLGQFKNALAATIIILCANILLREAAQRINQLPISAEGEKRYILKVTCNKEDESAVRQWLLNIVKEAAICLQGLGSVPAQEQGYKEIRAELVGHADYRKTRELIISRIGDNDNITAIHWSIDSQ 247DB4DCC3BFDB3D a0a0f6b8r9_salt1 Magnesium-transporting ATPase, P-type 1 mgtB Mg(2+) transport ATPase, P-type 1 STM14_4536 protein protein salt1 Salmonella typhimurium (strain 14028s / SGSC 2262) MTDMNIENRKLNRPASENDKQHKKVFPIEAEAFHSPEETLARLNSHRQGLTIEEASERLKVYGRNEVAHEQVPPALIQLLQAFNNPFIYVLMALAGVSFITDYWLPLRRGEETDLTGVLIILTMVSLSGLLRFWQEFRTNRAAQALKKMVRTTATVLRRGPGNIGAVQEEIPIEELVPGDVVFLAAGDLVPADVRLLASRDLFISQSILSGESLPVEKYDVMADVAGKDSEQLPDKDKSLLDLGNICLMGTNVTSGRAQAVVVATGSRTWFGSLAKSIVGTRTQTAFDRGVNSVSWLLIRFMLIMVPVVLLINGFSKGDWVEASLFALAVAVGLTPEMLPMIVSSNLAKGAIAMSRRKVIVKRLNAIQNFGAMDVLCTDKTGTLTQDNIFLEHHLDVSGVKSSRVLMLAWLNSSSQSGARNVMDRAILRFGEGRIAPSTKARFIKRDELPFDFVRRRVSVLVEDAQHGDRCLICKGAVEEMMMVATHLREGDRVVALTETRRELLLAKTEDYNAQGFRVLLIATRKLDGSGNNPTLSVEDETELTIEGMLTFLDPPKESAGKAIAALRDNGVAVKVLTGDNPVVTARICLEVGIDTHDILTGTQVEAMSDAELASEVEKRAVFARLTPLQKTRILQALQKNGHTVGFLGDGINDAPALRDADVGISVDSAADIAKESSDIILLEKDLMVLEEGVIKGRETFGNIIKYLNMTASSNFGNVFSVLVASAFIPFLPMLAIHLLIQNLMYDISQLSLPWDKMDKEFLRKPRKWDAKNIGRFMLWIGPTSSIFDITTFALMWYVFAANNVEAQALFQSGWFIEGLLSQTLVVHMLRTQKIPFIQSRATLPVLLTTGLIMAIGIYIPFSPLGAMVGLEPLPLSYFPWLVATLLSYCLVAQGMKRFYIKRFGQWF 9C5451BC6E2BA7C2 q8z2e7_salti G4Y55_000607 G7161_003210 G9264_003958 G9C68_003135 G9X37_002783 GND71_000122 G2244_00610 G3982_004372 G3V49_003652 G4I71_000155 G4L28_004359 G4P30_000122 G2227_03055 G4Y41_000019 CAJ76_00605 CAJ80_00125 CAJ85_14310 CAL67_17060 CB224_00335 CC884_15735 CC907_13375 CC972_01005 D4Z37_15570 D6Q30_19775 DKA88_14960 DNM71_00625 DNV09_09405 DS261_00625 DUQ91_00625 DUV11_00625 DVG36_13630 EBC38_00650 EID90_15455 ELQ62_12450 EU445_00610 EVI00_03195 F9R11_03150 F9Y21_03110 FI137_18190 GDI29_03150 ZZ17_00010 STY4100 t3823 protein protein salti Salmonella typhi MMNNARINQPIFAGASDVKRTKSPRAPTLEDVARSAGLSPMTVSRALNSPQLVRPKTVEKVMQAVRVTGYIPNALAGGLASRRSKLIAVVVPQINNNMFVDTIQSLSDELARRGYHILLCVAGYTEQTEAELVATLLSRRPDGVVLTGIHHTIELKKVILNAAIPVVEIWDLTPTPLDMLVGFSHEKVGQATGEYLLSKGYRRPGLLWTADRRAAQRKQGLCSVLQRHAIHAVPQVDVPLPASLSLGRSGLSQLFDEGTFDVIVCSSDTLAQGAMMEAESRGLRIPHDLAVIGFGDLDFAASNRPSITTVSVDRRAIGQRAATLLADRIEQKPCAEAIVDIGFHLIERESA 6589EEED64F394E0 a0a0f6b781_salt1 ATP-dependent zinc metalloprotease FtsH hflB ftsH STM14_3981 protein protein salt1 Salmonella typhimurium (strain 14028s / SGSC 2262) MAKNLILWLVIAVVLMSVFQSFGPSESNGRKVDYSTFLQEVNQDQVREARINGREINVTKKDSNRYTTYIPINDPKLLDNLLTKNVKVVGEPPEEPSLLASIFISWFPMLLLIGVWIFFMRQMQGGGGKGAMSFGKSKARMLTEDQIKTTFADVAGCDEAKEEVAELVEYLREPSRFQKLGGKIPKGVLMVGPPGTGKTLLAKAIAGEAKVPFFTISGSDFVEMFVGVGASRVRDMFEQAKKAAPCIIFIDEIDAVGRQRGAGLGGGHDEREQTLNQMLVEMDGFEGNEGIIVIAATNRPDVLDPALLRPGRFDRQVVVGLPDVRGREQILKVHMRRVPLATDIDAAIIARGTPGFSGADLANLVNEAALFAARGNKRVVSMVEFEKAKDKIMMGAERRSMVMTEAQKESTAYHEAGHAIIGRLVPEHDPVHKVTIIPRGRALGVTFFLPEGDAISASRQKLESQISTLYGGRLAEEIIYGVEHVSTGASNDIKVATNLARNMVTQWGFSEKLGPLLYAEEEGEVFLGRSVAKAKHMSDETARIIDQEVKALIERNYNRARQILTDNMDILHAMKDALMKYETIDAPQIDDLMARREVRPPAGWEDPNGTNNSDSNGTPQAPRPVDEPRTPNPGNTMSEQLGDK 0F4195A2CB40CF98 a0a0f6b941_salt1 ATP synthase subunit a atpB ATP synthase F0 sector subunit a F-ATPase subunit 6 STM14_4667 protein protein salt1 Salmonella typhimurium (strain 14028s / SGSC 2262) MASENMTPQEYIGHHLNNLQLDLRTFSLVDPQNPPATFWTLNIDSMFFSVVLGLLFLVMFRSVAKKATSGVPGKFQTAIELIVGFVHGSVKDMYHGKSKLIAPLALTIFVWVFLMNLMDLLPIDLLPYIAEHWLGLPATRVVPSADVNITLSMALGVFILILFYSIKMKGIGGFAKELTLQPFNHWAFIPVNLILEGVSLLSKPVSLGLRLFGNMYAGELIFILIAGLLPWWSQWILNVPWAIFHILIITLQAFIFMVLTIVYLSMASEEH 4A5FAA208BC75E9B phop_salt1 Virulence transcriptional regulatory protein PhoP phoP STM14_1409 protein protein salt1 Salmonella typhimurium (strain 14028s / SGSC 2262) MMRVLVVEDNALLRHHLKVQLQDSGHQVDAAEDAREADYYLNEHLPDIAIVDLGLPDEDGLSLIRRWRSSDVSLPVLVLTAREGWQDKVEVLSSGADDYVTKPFHIEEVMARMQALMRRNSGLASQVINIPPFQVDLSRRELSVNEEVIKLTAFEYTIMETLIRNNGKVVSKDSLMLQLYPDAELRESHTIDVLMGRLRKKIQAQYPHDVITTVRGQGYLFELR 3A90B2FDC328C7B2 mgtc-atpb-3 2 8 unspecified role unspecified role prey prey 4 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 11 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Supp. Fig 1A mgtc-atpb-1 3 6 unspecified role unspecified role bait bait c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role prey prey c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Supp. Fig 2A mgtc-atpb-2 1 10 unspecified role unspecified role prey prey c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range in vitro In vitro 6 unspecified role unspecified role bait bait D0ZLQ7:p.Asn92Thr mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain N T Wed May 02 19:24:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn92thr' Tue Jun 30 16:33:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn92Thr' c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range in vitro In vitro direct interaction direct interaction Fig. 1, Supp Fig. 3 mgtc-atpb-2 1 10 unspecified role unspecified role bait bait c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range in vitro In vitro 6 unspecified role unspecified role prey prey c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range in vitro In vitro direct interaction direct interaction Fig. 1, Supp. Fig 3 atpb-mgtc 3 10 unspecified role unspecified role bait bait c-terminal ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range 6 unspecified role unspecified role prey prey c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Supp. Fig 2A