MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it A glycolytic metabolon in Saccharomyces cerevisiae is stabilized by F-actin. A glycolytic metabolon in Saccharomyces cerevisiae is stabilized by F-actin. FEBS J. (1742-464X) 2013 Araiza-Olivera D., Chiquete-Felix N., Rosas-Lemus M., Sampedro JG., Peña A., Mujica A., Uribe-Carvajal S. suggested by lperfetto yeast Saccharomyces cerevisiae Baker's yeast anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Araiza-Olivera D., Chiquete-Felix N., Rosas-Lemus M., Sampedro JG., Peña A., Mujica A., Uribe-Carvajal S. suggested by lperfetto FEBS J. (1742-464X) 2013 A glycolytic metabolon in Saccharomyces cerevisiae is stabilized by F-actin. A glycolytic metabolon in Saccharomyces cerevisiae is stabilized by F-actin. FEBS J. (1742-464X) 2013 Araiza-Olivera D., Chiquete-Felix N., Rosas-Lemus M., Sampedro JG., Peña A., Mujica A., Uribe-Carvajal S. suggested by lperfetto in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom western blot western blot Immuno blot Immuno blot Araiza-Olivera D., Chiquete-Felix N., Rosas-Lemus M., Sampedro JG., Peña A., Mujica A., Uribe-Carvajal S. suggested by lperfetto FEBS J. (1742-464X) 2013 g3p1_yeast Glyceraldehyde-3-phosphate dehydrogenase 1 TDH1 GPD1 SSS2 YJL052W J1154 protein protein yeast Saccharomyces cerevisiae Baker's yeast MIRIAINGFGRIGRLVLRLALQRKDIEVVAVNDPFISNDYAAYMVKYDSTHGRYKGTVSHDDKHIIIDGVKIATYQERDPANLPWGSLKIDVAVDSTGVFKELDTAQKHIDAGAKKVVITAPSSSAPMFVVGVNHTKYTPDKKIVSNASCTTNCLAPLAKVINDAFGIEEGLMTTVHSMTATQKTVDGPSHKDWRGGRTASGNIIPSSTGAAKAVGKVLPELQGKLTGMAFRVPTVDVSVVDLTVKLEKEATYDQIKKAVKAAAEGPMKGVLGYTEDAVVSSDFLGDTHASIFDASAGIQLSPKFVKLISWYDNEYGYSARVVDLIEYVAKA 4C116C86EAF3DB70 4C116C86EAF3DB70 alf_yeast Fructose-bisphosphate aldolase FBA1 YKL320 Fructose-1,6-bisphosphate aldolase YKL060C protein protein yeast Saccharomyces cerevisiae Baker's yeast MGVEQILKRKTGVIVGEDVHNLFTYAKEHKFAIPAINVTSSSTAVAALEAARDSKSPIILQTSNGGAAYFAGKGISNEGQNASIKGAIAAAHYIRSIAPAYGIPVVLHSDHCAKKLLPWFDGMLEADEAYFKEHGEPLFSSHMLDLSEETDEENISTCVKYFKRMAAMDQWLEMEIGITGGEEDGVNNENADKEDLYTKPEQVYNVYKALHPISPNFSIAAAFGNCHGLYAGDIALRPEILAEHQKYTREQVGCKEEKPLFLVFHGGSGSTVQEFHTGIDNGVVKVNLDTDCQYAYLTGIRDYVLNKKDYIMSPVGNPEGPEKPNKKFFDPRVWVREGEKTMGAKITKSLETFRTTNTL C67E61BA5C7E8E4C C67E61BA5C7E8E4C pgk_yeast Phosphoglycerate kinase PGK1 YCR12W YCR012W protein protein yeast Saccharomyces cerevisiae Baker's yeast MSLSSKLSVQDLDLKDKRVFIRVDFNVPLDGKKITSNQRIVAALPTIKYVLEHHPRYVVLASHLGRPNGERNEKYSLAPVAKELQSLLGKDVTFLNDCVGPEVEAAVKASAPGSVILLENLRYHIEEEGSRKVDGQKVKASKEDVQKFRHELSSLADVYINDAFGTAHRAHSSMVGFDLPQRAAGFLLEKELKYFGKALENPTRPFLAILGGAKVADKIQLIDNLLDKVDSIIIGGGMAFTFKKVLENTEIGDSIFDKAGAEIVPKLMEKAKAKGVEVVLPVDFIIADAFSADANTKTVTDKEGIPAGWQGLDNGPESRKLFAATVAKAKTIVWNGPPGVFEFEKFAAGTKALLDEVVKSSAAGNTVIIGGGDTATVAKKYGVTDKISHVSTGGGASLELLEGKELPGVAFLSEKK 378AB944DB34EFF0 378AB944DB34EFF0 fba1-pgk1 2 PGK 5 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level GAPDH 3 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level ALD 4 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait purified purified association association f1a mint In the bound fraction, GAPDH, PGK, ALD and actin were detected (Fig. 1A), while the proteins we used as negative controls, ubiquitin and Hog1 were in the free fraction (Fig. 1B). Thus, immobilized S. cerevisiae-ALD retained actin and several yeast glycolytic enzymes, suggesting the existence of an actin-bound glycolytic complex fba1-pgk1-1 1 GAPDH 3 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level ALD 4 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level PGK 5 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep physiological level physiological level endogenous level endogenous endogenous endogenous level association association mint f4a f4c