IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Construction of a novel oligonucleotide array-based transcription factor interaction assay platform and its uses for profiling STAT1 cofactors in mouse fibroblast cells Construction of a novel oligonucleotide array-based transcription factor interaction assay platform and its uses for profiling STAT1 cofactors in mouse fibroblast cells Proteomics (1615-9853) 2013 imex curation Zeng L., Sun Y., Xie L., Wei L., Ren Y., Zhao J., Qin W., Mitchelson K., Cheng J. The authors state thalis3t3 and 3t3-L1 were used in the publication, but no details as to which cell line was used in which experiment were given. 2013-03-01: Y Sun, National Engineering Research Center for Beijing Biochip Technology, Beijing. can be checked soonish.. Manuscript draft and other information concerning this submission can be found in ebi/sp/pro6/intact/local/data/curation-material/2013/sun-2013/ Interactions detected using Targeted Immuno-Co-precipitation - Oligonucleotide Array-based TF Assay (TIC-OATFA). Similar to OATFA, the TIC-OATFA assay also involves use of a set of various cis-DNA element sequences as probes that can be bound by their corresponding active TFs in a nuclear extract. In detail, the addition of antibody against a specific target TF results in the co-precipitation of other TF proteins associated in a complex with the target. The cis-element DNA sequence probes which are bound by the individual TFs are also precipitated in such complexes, whilst any unbound probes are eliminated. The TF bound DNA probes are then purified, amplified, and analyzed by array hybridization to determine both their identity and abundance. The targeted immuno-co-precipitation step ensures the identity of the principal TF which is under examination, whilst the co-precipitated active TFs are identified by OATFA assay. A nuclear extract from IFN-gamma treated cells was added to a Probe Mix (a set of double-stranded oligonucleotide probes that contain a known library of cis-elements). After immunoprecipitation with anti-STAT1, bound DNA probes were recovered and amplified using a Cy5 labeled primer. Hybridisation to a DNA microarray (TIC-OATFA platform containing 240 consensus TF binding sequences) was carried out. Fluorescent labels were detected using a scanner (LuxScan). Interaction including NFkB and NFAT could not be included, since antibodies cannot ascertain the identity of the proteins within their families. Information about usf1-stat1 interaction was provided by the authors upon email request. ymsun@capitalbio.com 19-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells anti bait coip anti bait coimmunoprecipitation anti bait coip tag fluorescence tag visualisation by fluorescence tag fluorescence Zeng L., Sun Y., Xie L., Wei L., Ren Y., Zhao J., Qin W., Mitchelson K., Cheng J. The authors state thalis3t3 and 3t3-L1 were used in the publication, but no details as to which cell line was used in which experiment were given. 2013-03-01: Y Sun, National Engineering Research Center for Beijing Biochip Technology, Beijing. can be checked soonish.. Interactions detected using Targeted Immuno-Co-precipitation - Oligonucleotide Array-based TF Assay (TIC-OATFA). Similar to OATFA, the TIC-OATFA assay also involves use of a set of various cis-DNA element sequences as probes that can be bound by their corresponding active TFs in a nuclear extract. In detail, the addition of antibody against a specific target TF results in the co-precipitation of other TF proteins associated in a complex with the target. The cis-element DNA sequence probes which are bound by the individual TFs are also precipitated in such complexes, whilst any unbound probes are eliminated. The TF bound DNA probes are then purified, amplified, and analyzed by array hybridization to determine both their identity and abundance. The targeted immuno-co-precipitation step ensures the identity of the principal TF which is under examination, whilst the co-precipitated active TFs are identified by OATFA assay. A nuclear extract from IFN-gamma treated cells was added to a Probe Mix (a set of double-stranded oligonucleotide probes that contain a known library of cis-elements). After immunoprecipitation with anti-STAT1, bound DNA probes were recovered and amplified using a Cy5 labeled primer. Hybridisation to a DNA microarray (TIC-OATFA platform containing 240 consensus TF binding sequences) was carried out. Fluorescent labels were detected using a scanner (LuxScan). Interaction including NFkB and NFAT could not be included, since antibodies cannot ascertain the identity of the proteins within their families. Information about usf1-stat1 interaction was provided by the authors upon email request. ymsun@capitalbio.com Accepted 2013-APR-03 by MGT 19-Jun-2013: Contacted by IntAct-Help. Proteomics (1615-9853) 2013 Only protein-protein interactions imex curation Construction of a novel oligonucleotide array-based transcription factor interaction assay platform and its uses for profiling STAT1 cofactors in mouse fibroblast cells Construction of a novel oligonucleotide array-based transcription factor interaction assay platform and its uses for profiling STAT1 cofactors in mouse fibroblast cells Proteomics (1615-9853) 2013 imex curation Zeng L., Sun Y., Xie L., Wei L., Ren Y., Zhao J., Qin W., Mitchelson K., Cheng J. The authors state thalis3t3 and 3t3-L1 were used in the publication, but no details as to which cell line was used in which experiment were given. 2013-03-01: Y Sun, National Engineering Research Center for Beijing Biochip Technology, Beijing. can be checked soonish.. Manuscript draft and other information concerning this submission can be found in ebi/sp/pro6/intact/local/data/curation-material/2013/sun-2013/ Interactions detected using Targeted Immuno-Co-precipitation - Oligonucleotide Array-based TF Assay (TIC-OATFA). Similar to OATFA, the TIC-OATFA assay also involves use of a set of various cis-DNA element sequences as probes that can be bound by their corresponding active TFs in a nuclear extract. In detail, the addition of antibody against a specific target TF results in the co-precipitation of other TF proteins associated in a complex with the target. The cis-element DNA sequence probes which are bound by the individual TFs are also precipitated in such complexes, whilst any unbound probes are eliminated. The TF bound DNA probes are then purified, amplified, and analyzed by array hybridization to determine both their identity and abundance. The targeted immuno-co-precipitation step ensures the identity of the principal TF which is under examination, whilst the co-precipitated active TFs are identified by OATFA assay. A nuclear extract from IFN-gamma treated cells was added to a Probe Mix (a set of double-stranded oligonucleotide probes that contain a known library of cis-elements). After immunoprecipitation with anti-STAT1, bound DNA probes were recovered and amplified using a Cy5 labeled primer. Hybridisation to a DNA microarray (TIC-OATFA platform containing 240 consensus TF binding sequences) was carried out. Fluorescent labels were detected using a scanner (LuxScan). Interaction including NFkB and NFAT could not be included, since antibodies cannot ascertain the identity of the proteins within their families. Information about usf1-stat1 interaction was provided by the authors upon email request. ymsun@capitalbio.com 19-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Zeng L., Sun Y., Xie L., Wei L., Ren Y., Zhao J., Qin W., Mitchelson K., Cheng J. The authors state thalis3t3 and 3t3-L1 were used in the publication, but no details as to which cell line was used in which experiment were given. 2013-03-01: Y Sun, National Engineering Research Center for Beijing Biochip Technology, Beijing. can be checked soonish.. Interactions detected using Targeted Immuno-Co-precipitation - Oligonucleotide Array-based TF Assay (TIC-OATFA). Similar to OATFA, the TIC-OATFA assay also involves use of a set of various cis-DNA element sequences as probes that can be bound by their corresponding active TFs in a nuclear extract. In detail, the addition of antibody against a specific target TF results in the co-precipitation of other TF proteins associated in a complex with the target. The cis-element DNA sequence probes which are bound by the individual TFs are also precipitated in such complexes, whilst any unbound probes are eliminated. The TF bound DNA probes are then purified, amplified, and analyzed by array hybridization to determine both their identity and abundance. The targeted immuno-co-precipitation step ensures the identity of the principal TF which is under examination, whilst the co-precipitated active TFs are identified by OATFA assay. A nuclear extract from IFN-gamma treated cells was added to a Probe Mix (a set of double-stranded oligonucleotide probes that contain a known library of cis-elements). After immunoprecipitation with anti-STAT1, bound DNA probes were recovered and amplified using a Cy5 labeled primer. Hybridisation to a DNA microarray (TIC-OATFA platform containing 240 consensus TF binding sequences) was carried out. Fluorescent labels were detected using a scanner (LuxScan). Interaction including NFkB and NFAT could not be included, since antibodies cannot ascertain the identity of the proteins within their families. Information about usf1-stat1 interaction was provided by the authors upon email request. ymsun@capitalbio.com Accepted 2013-APR-03 by MGT 19-Jun-2013: Contacted by IntAct-Help. Proteomics (1615-9853) 2013 Only protein-protein interactions imex curation Construction of a novel oligonucleotide array-based transcription factor interaction assay platform and its uses for profiling STAT1 cofactors in mouse fibroblast cells Construction of a novel oligonucleotide array-based transcription factor interaction assay platform and its uses for profiling STAT1 cofactors in mouse fibroblast cells Proteomics (1615-9853) 2013 imex curation Zeng L., Sun Y., Xie L., Wei L., Ren Y., Zhao J., Qin W., Mitchelson K., Cheng J. The authors state thalis3t3 and 3t3-L1 were used in the publication, but no details as to which cell line was used in which experiment were given. 2013-03-01: Y Sun, National Engineering Research Center for Beijing Biochip Technology, Beijing. can be checked soonish.. Manuscript draft and other information concerning this submission can be found in ebi/sp/pro6/intact/local/data/curation-material/2013/sun-2013/ Interactions detected using Targeted Immuno-Co-precipitation - Oligonucleotide Array-based TF Assay (TIC-OATFA). Similar to OATFA, the TIC-OATFA assay also involves use of a set of various cis-DNA element sequences as probes that can be bound by their corresponding active TFs in a nuclear extract. In detail, the addition of antibody against a specific target TF results in the co-precipitation of other TF proteins associated in a complex with the target. The cis-element DNA sequence probes which are bound by the individual TFs are also precipitated in such complexes, whilst any unbound probes are eliminated. The TF bound DNA probes are then purified, amplified, and analyzed by array hybridization to determine both their identity and abundance. The targeted immuno-co-precipitation step ensures the identity of the principal TF which is under examination, whilst the co-precipitated active TFs are identified by OATFA assay. A nuclear extract from IFN-gamma treated cells was added to a Probe Mix (a set of double-stranded oligonucleotide probes that contain a known library of cis-elements). After immunoprecipitation with anti-STAT1, bound DNA probes were recovered and amplified using a Cy5 labeled primer. Hybridisation to a DNA microarray (TIC-OATFA platform containing 240 consensus TF binding sequences) was carried out. Fluorescent labels were detected using a scanner (LuxScan). Interaction including NFkB and NFAT could not be included, since antibodies cannot ascertain the identity of the proteins within their families. Information about usf1-stat1 interaction was provided by the authors upon email request. ymsun@capitalbio.com 19-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Zeng L., Sun Y., Xie L., Wei L., Ren Y., Zhao J., Qin W., Mitchelson K., Cheng J. The authors state thalis3t3 and 3t3-L1 were used in the publication, but no details as to which cell line was used in which experiment were given. 2013-03-01: Y Sun, National Engineering Research Center for Beijing Biochip Technology, Beijing. can be checked soonish.. Interactions detected using Targeted Immuno-Co-precipitation - Oligonucleotide Array-based TF Assay (TIC-OATFA). Similar to OATFA, the TIC-OATFA assay also involves use of a set of various cis-DNA element sequences as probes that can be bound by their corresponding active TFs in a nuclear extract. In detail, the addition of antibody against a specific target TF results in the co-precipitation of other TF proteins associated in a complex with the target. The cis-element DNA sequence probes which are bound by the individual TFs are also precipitated in such complexes, whilst any unbound probes are eliminated. The TF bound DNA probes are then purified, amplified, and analyzed by array hybridization to determine both their identity and abundance. The targeted immuno-co-precipitation step ensures the identity of the principal TF which is under examination, whilst the co-precipitated active TFs are identified by OATFA assay. A nuclear extract from IFN-gamma treated cells was added to a Probe Mix (a set of double-stranded oligonucleotide probes that contain a known library of cis-elements). After immunoprecipitation with anti-STAT1, bound DNA probes were recovered and amplified using a Cy5 labeled primer. Hybridisation to a DNA microarray (TIC-OATFA platform containing 240 consensus TF binding sequences) was carried out. Fluorescent labels were detected using a scanner (LuxScan). Interaction including NFkB and NFAT could not be included, since antibodies cannot ascertain the identity of the proteins within their families. Information about usf1-stat1 interaction was provided by the authors upon email request. ymsun@capitalbio.com Accepted 2013-APR-03 by MGT 19-Jun-2013: Contacted by IntAct-Help. Proteomics (1615-9853) 2013 Only protein-protein interactions imex curation stat1_mouse Signal transducer and activator of transcription 1 Stat1 protein protein mouse Mus musculus Mouse MSQWFELQQLDSKFLEQVHQLYDDSFPMEIRQYLAQWLEKQDWEHAAYDVSFATIRFHDLLSQLDDQYSRFSLENNFLLQHNIRKSKRNLQDNFQEDPVQMSMIIYNCLKEERKILENAQRFNQAQEGNIQNTVMLDKQKELDSKVRNVKDQVMCIEQEIKTLEELQDEYDFKCKTSQNREGEANGVAKSDQKQEQLLLHKMFLMLDNKRKEIIHKIRELLNSIELTQNTLINDELVEWKRRQQSACIGGPPNACLDQLQTWFTIVAETLQQIRQQLKKLEELEQKFTYEPDPITKNKQVLSDRTFLLFQQLIQSSFVVERQPCMPTHPQRPLVLKTGVQFTVKSRLLVKLQESNLLTKVKCHFDKDVNEKNTVKGFRKFNILGTHTKVMNMEESTNGSLAAELRHLQLKEQKNAGNRTNEGPLIVTEELHSLSFETQLCQPGLVIDLETTSLPVVVISNVSQLPSGWASILWYNMLVTEPRNLSFFLNPPCAWWSQLSEVLSWQFSSVTKRGLNADQLSMLGEKLLGPNAGPDGLIPWTRFCKENINDKNFSFWPWIDTILELIKNDLLCLWNDGCIMGFISKERERALLKDQQPGTFLLRFSESSREGAITFTWVERSQNGGEPDFHAVEPYTKKELSAVTFPDIIRNYKVMAAENIPENPLKYLYPNIDKDHAFGKYYSRPKEAPEPMELDDPKRTGYIKTELISVSEVHPSRLQTTDNLLPMSPEEFDEMSRIVGPEFDSMMSTV DIP protein P42225 original sequence version: 131 DIP protein P42225 original sequence version: 71 249D919952BE65F1 usf1_mouse Upstream stimulatory factor 1 Usf1 Usf Major late transcription factor 1 protein protein mouse Mus musculus Mouse MKGQQKTAETEEGTVQIQEGAVATGEDPTSVAIASIQSAATFPDPNVKYVFRTENGGQVMYRVIQVSEGQLDGQTEGSGAISGYPATQSMTQAVIQGAFTSDDAVDTEGAAAETHYTYFPSTAVGDGSGGTTSGSTTAVVTTQGSEALLGQATPPSTGQFFVMMSPQEVLQGGSQRSIAPRTHPYSPKSEAPRTTRDEKRRAQHNEVERRRRDKINNWIVQLSKIIPDCSMESTKSGQSKGGILSKACDYIQELRQSNHRLSEELQGLDQLQLDNDVLRQQVEDLKNKNLLLRAQLRHHGLEVVIKNDSN 7D0AF808A2FDFA1C nf1_mouse Neurofibromin Nf1 Neurofibromatosis-related protein NF-1 protein protein mouse Mus musculus Mouse MAAHRPVEWVQAVVSRFDEQLPIKTGQQNTHTKVSTEHNKECLINISKYKFSLVISGLTTILKNVNNMRIFGEAAEKNLYLSQLIILDTLEKCLAGQPKDTMRLDETMLVKQLLPEICHFLHTCREGNQHAAELRNSASGVLFSLSCNNFNAVFSRISTRLQELTVCSEDNVDVHDIELLQYINVDCAKLKRLLKETAFKFKALKKVAQLAVINSLEKAFWNWVENYPDEFTKLYQIPQTDMAECAEKLFDLVDGFAESTKRKAAVWPLQIILLILCPEIIQDISKDVVDESNINKKLFLDSLRKALAGHGGSRQLTESAAIACVKLCKASTYINWEDNSVIFLLVQSMVVDLKNLLFNPSKPFSRGSQPADVDLMIDCLVSCFRISPHNNQHFKICLAQNSPSTFHYVLVNSLHRIITNSALDWWPKIDAVYCHSVELRNMFGETLHKAVQGCGAHPAIRMAPSLTFKEKVTSLKFKEKPTDLETRSYKCLLLSMVKLIHADPKLLLCNPRKQGPETQSSTAELITGLVQLVPQSHMPEVAQEAMEALLVLHQLDSIDLWNPDAPVETFWEISSQMLFYICKKLTSHQMLSSTEILKWLREILICRNKFLLKNKQADRSSCHSLYLYGVGCEMSATGNTTQMSVDHDEFLRACTPGASLRKGRGNSSMDSTAGCSGTPPICRQAQTKLEVALYMFLWNPDTEAVLVAMSCFRHLCEEADIRCGVDEVSVHNFLPNYNTFMEFASVSNMMSTGRAALQKRVMALLRRIEHPTAGNIEAWEDTHAKWEQATKLILNYPKAKMEDGQAAESLHKTIVKRRMSHVSGGGSIDLSDTDSLQEWINMTGFLCALGGVCLQQRSSSGLATYSPPMGAVSERKGSMISVMSSEGNIDSPVSRFMDRLLSLMVCNHEKVGLQIRTNVKDLVGLELSPALYPMLFNKLKNTISKFFDSQGQVLLSDSNTQFVEQTIAIMKNLLDNHTEGSSEHLGQASIETMMLNLVRYVRVLGNMVHAIQIKTKLCQLVEVMMARRDDLSFCQEMKFRNKMVEYLTDWVMGTSNQAADDDIKCLTRDLDQASMEAVVSLLAGLPLQPEEGDGVELMEAKSQLFLKYFTLFMNLLNDCSEVEDENAQTGGRKRGMSRRLASLRHCTVLAMSNLLNANVDSGLMHSIGLGYHKDLQTRATFMEVLTKILQQGTEFDTLAETVLADRFERLVELVTMMGDQGELPIAMALANVVPCSQWDELARVLVTLFDSRHLLYQLLWNMFSKEVELADSMQTLFRGNSLASKIMTFCFKVYGATYLQKLLDPLLRVIITSSDWQHVSFEVDPTRLEPSESLEENQRNLLQMTEKFFHAIISSSSEFPSQLRSVCHCLYQATCHSLLNKATVKERKENKKSVVSQRFPQNSIGAVGSAMFLRFINPAIVSPYEAGILDKKPPPRIERGLKLMSKVLQSIANHVLFTKEEHMRPFNDFVKSNFDLARRFFLDIASDCPTSDAVNHSLSFISDGNVLALHRLLWNNQEKIGQYLSSNRDHKAVGRRPFDKMATLLAYLGPPEHKPVADTHWSSLNLTSSKFEEFMTRHQVHEKEEFKALKTLSIFYQAGTSKAGNPIFYYVARRFKTGQINGDLLIYHVLLTLKPYYAKPYEIVVDLTHTGPSNRFKTDFLSKWFVVFPGFAYDNVSAVYIYNCNSWVREYTKYHERLLTGLKGSKRLIFIDCPGKLAEHIEHEQQKLPAATLALEEDLKVFHNALKLAHKDTKVSIKVGSTAVQVTSAERTKVLGQSVFLNDIYYASEIEEICLVDENQFTLTIANQGTPLTFMHQECEAIVQSIIHIRTRWELSQPDSIPQHTKIRPKDVPGTLLNIALLNLGSSDPSLRSAAYNLLCALTCTFNLKIEGQLLETSGLCIPANNTLFIVSISKTLAANEPHLTLEFLEECISGFSKSSIELKHLCLEYMTPWLSNLVRFCKHNDDAKRQRVTAILDKLITMTINEKQMYPSIQAKIWGSLGQITDLLDVVLDSFIKTSATGGLGSIKAEVMADTAVALASGNVKLVSSKVIGRMCKIIDKTCLSPTPTLEQHLMWDDIAILARYMLMLSFNNSLDVAAHLPYLFHVVTFLVATGPLSLRASTHGLLINIIHSLCTCSQLHFSEETKQVLRLSLTEFSLPKFYLLFGISKVKSAAVIAFRSSYRDRSFSPGSYERETFALTSLETVTEALLEIMEACMRDIPTCKWLDQWTELAQRFAFQYNPSLQPRALVVFGCISKRVSHGQIKQIIRILSKALESCLKGPDTYNSQVLIESTVIALTKLQPLLNKDSPLHKALFWVAVAVLQLDEVNLYSAGTALLEQNLHTLDSLRIFNDKSPEEVFMAIRNPLEWHCKQMDHFVGLNFNSNFNFALVGHLLKGYRHPSPAIVARTVRILHTLLTLVNKHRNCDKFEVNTQSVAYLAALLTVSEEVRSRCSLKHRKSLLLTDISMENVPMDTYPIHHGDPSYRTLKETQPWSSPKGSEGYLAATYPAVGQTSPRARKSMSLDMGQPSQANTKKLLGTRKSFDHLISDTKAPKRQEMESGITTPPKMRRVAETDYEMETQRIPSSQQHPHLRKVSVSESNVLLDEEVLTDPKIQALLLTVLATLVKYTTDEFDQRILYEYLAEASVVFPKVFPVVHNLLDSKINTLLSLCQDPNLLNPIHGIVQSVVYHEESPPQYQTSYLQSFGFNGLWRFAGPFSKQTQIPDYAELIVKFLDALIDTYLPGIDEETSEESLLTPTSPYPPALQSQLSITANLNLSNSMTSLATSQHSPGLDKENVELSPTAGHCNSGRTRHGSASQVQKQRSAGSFKRNSIKKIV 06650BDCDC531002 tbp_mouse TATA-box-binding protein Tbp Tfiid TATA sequence-binding protein TATA-binding factor TATA-box factor Transcription initiation factor TFIID TBP subunit protein protein mouse Mus musculus Mouse MDQNNSLPPYAQGLASPQGAMTPGIPIFSPMMPYGTGLTPQPIQNTNSLSILEEQQRQQQQQQQQQQQQQAVATAAASVQQSTSQQPTQGASGQTPQLFHSQTLTTAPLPGTTPLYPSPMTPMTPITPATPASESSGIVPQLQNIVSTVNLGCKLDLKTIALRARNAEYNPKRFAAVIMRIREPRTTALIFSSGKMVCTGAKSEEQSRLAARKYARVVQKLGFPAKFLDFKIQNMVGSCDVKFPIRLEGLVLTHQQFSSYEPELFPGLIYRMIKPRIVLLIFVSGKVVLTGAKVRAEIYEAFENIYPILKGFRKTT A33D776160B4A393 usf2_mouse Upstream stimulatory factor 2 Upstream transcription factor 2 Major late transcription factor 2 Usf2 protein protein mouse Mus musculus Mouse MDMLDPGLDPASSATAAAAASHDKGPEAEEGVELQEGGDGPGAEEQTAVAIASVQQAAFGDHNIQYQFRTESNGGQVTYRVVQVTDGQLDGQGDAAGAVSVVSTAAFAGGQQAVTQVGVDGAAQRPGPAAASVPTGPAAPFPLAVIQNPFSNGGSPAAEAVSGEARFAYFPASSVGDTTAVSVQTTDQSLQAGGQFYVMMTPQDVLQTGTQRTIAPRTHPYSPKIDGTRTPRDERRRAQHNEVERRRRDKINNWIVQLSKIIPDCHADNSKTGASKGGILSKACDYIRELRQTNQRMQETFKEAERLQMDNELLRQQIEELKNENALLRAQLQQHNLEMVGESTRQ E9D216BC25F9447B nfe2_mouse Transcription factor NF-E2 45 kDa subunit Nfe2 Leucine zipper protein NF-E2 Nuclear factor, erythroid-derived 2 45 kDa subunit p45 NF-E2 protein protein mouse Mus musculus Mouse MPPCPPQQNRNRLSQLPVGELGEMELTWQEIMSITELQGLNVPSETSFEPQAPTPYPGPLPPPTYCPCSIHPDAGFSLPPPSYELPASTPHVPELPYSYGNVAIPVSKPLTLSGLLNEPLPDHLALLDIGLPVGQPKPQEDPESDSGLSLNYSDAESLELEGMEAGRRRSEYVDMYPVEYPYSLMPNSLAHPNYTLPPTETPLALESSSGPVRAKPAVRGEAGSRDERRALAMKIPFPTDKIVNLPVDDFNELLAQYPLTESQLALVRDIRRRGKNKVAAQNCRKRKLETIVQLERELERLSSERERLLRARGEADRTLEVMRQQLAELYHDIFQHLRDESGNSYSPEEYVLQQAADGAIFLVPRGTKMEATD DIP protein Q07279 original sequence version: 72 DIP protein Q07279 original sequence version: 75 47E505F78B6EEB65 stat1-nf1 1 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role prey prey 8 unspecified role unspecified role prey prey 5 unspecified role unspecified role prey prey 4 unspecified role unspecified role bait bait association association 2a and 2b usf2-stat1 3 8 unspecified role unspecified role bait bait 4 unspecified role unspecified role prey prey physical association physical association 3c IFN-gamma usf2-stat1-1 2 8 unspecified role unspecified role bait bait purified purified n-terminal gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 4 unspecified role unspecified role prey prey subcellular prep subcellular preparation subcellular prep mouse-3t3 Mus musculus embryo cells 3t3 Mouse embryo fibroblast cells physical association physical association 3d usf1-stat1-2 3 4 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association 3c IFN-gamma