MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Tdrkh is essential for spermatogenesis and participates in primary piRNA biogenesis in the germline. Tdrkh is essential for spermatogenesis and participates in primary piRNA biogenesis in the germline. EMBO J. (0261-4189) 2013 imex curation Saxe J.P., Chen M., Zhao H., Lin H. haifan.lin@yale.edu 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions mouse-testis Mus musculus testis testis anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Saxe J.P., Chen M., Zhao H., Lin H. haifan.lin@yale.edu Accepted 2014-JAN-27 by MARTA EMBO J. (0261-4189) 2013 Only protein-protein interactions imex curation piwl2_mouse Piwi-like protein 2 Piwil2 Mili protein protein mouse Mus musculus Mouse MDPVRPLFRGPTPVHPSQCVRMPGCWPQAPRPLEPAWGRAGPAGRGLVFRKPEDSSPPLQPVQKDSVGLVSMFRGMGLDTAFRPPSKREVPPLGRGVLGRGLSANMVRKDREEPRSSLPDPSVLAAGDSKLAEASVGWSRMLGRGSSEVSLLPLGRAASSIGRGMDKPPSAFGLTARDPPRLPQPPALSPTSLHSADPPPVLTMERKEKELLVKQGSKGTPQSLGLNLIKIQCHNEAVYQYHVTFSPSVECKSMRFGMLKDHQSVTGNVTAFDGSILYLPVKLQQVVELKSQRKTDDAEISIKIQLTKILEPCSDLCIPFYNVVFRRVMKLLDMKLVGRNFYDPTSAMVLQQHRLQIWPGYAASIRRTDGGLFLLADVSHKVIRNDSVLDVMHAIYQQNKEHFQDECSKLLVGSIVITRYNNRTYRIDDVDWNKTPKDSFVMSDGKEITFLEYYSKNYGITVKEDDQPLLIHRPSERQNNHGMLLKGEILLLPELSFMTGIPEKMKKDFRAMKDLTQQINLSPKQHHGALECLLQRISQNETASNELTRWGLSLHKDVHKIEGRLLPMERINLRNTSFVTSEDLNWVKEVTRDASILTIPMHFWALFYPKRAMDQARELVNMLEKIAGPIGMRISPPAWVELKDDRIETYIRTIQSLLGVEGKIQMVVCIIMGTRDDLYGAIKKLCCVQSPVPSQVINVRTIGQPTRLRSVAQKILLQMNCKLGGELWGVDIPLKQLMVIGMDVYHDPSRGMRSVVGFVASINLTLTKWYSRVVFQMPHQEIVDSLKLCLVGSLKKYYEVNHCLPEKIVVYRDGVSDGQLKTVANYEIPQLQKCFEAFDNYHPKMVVFVVQKKISTNLYLAAPDHFVTPSPGTVVDHTITSCEWVDFYLLAHHVRQGCGIPTHYICVLNTANLSPDHMQRLTFKLCHMYWNWPGTIRVPAPCKYAHKLAFLSGQILHHEPAIQLCGNLFFL DIP protein Q8CDG1 original sequence version: 50 mint 01E143C6513310FB tdrkh_mouse Tudor and KH domain-containing protein Tdrkh Tdrd2 Tudor domain-containing protein 2 protein protein mouse Mus musculus Mouse MSTERTSWTNLSTIQKIALGLGIPASATVAYILYRRYRESREERLTFVGEDDIEIEMRVPQEAVKLIIGRQGANIKQLRKQTGARIDVDTEDVGDERVLLISGFPVQVCKAKAAIHQILTENTPVFEQLSVPQRSVGRIIGRGGETIRSICKASGAKITCDKESEGTLLLSRLIKISGTQKEVAAAKHLILEKVSEDEELRKRIAHSAETRVPRKQPISVRREEVTEPGGAGEAALWKNTNSSMGPATPLEVPLRKGGGDMVVVGPKEGSWEKPNDDSFQNSGAQSSPETSMFEIPSPDFSFHADEYLEVYVSASEHPNHFWIQIIGSRSLQLDKLVSEMTQHYENSLPEDLTVHVGDIVAAPLSTNGSWYRARVLGTLENGNLDLYFVDFGDNGDCALKDLRALRSDFLSLPFQAIECSLARIAPTGEEWEEEALDEFDRLTHCADWKPLVAKISSYVQTGISTWPKIYLYDTSDEKKLDIGLELVRKGYAVELPEDMEENRTVPNMLKDMATETDDSLASILTETKKSPEEMPHTLSCLSLSEAASMSGDDNLEDDLF 57AB7B6F56D33128 piwl1_mouse Piwi-like protein 1 Piwil1 Miwi protein protein mouse Mus musculus Mouse MTGRARARARGRARGQETVQHVGAAASQQPGYIPPRPQQSPTEGDLVGRGRQRGMVVGATSKSQELQISAGFQELSLAERGGRRRDFHDLGVNTRQNLDHVKESKTGSSGIIVKLSTNHFRLTSRPQWALYQYHIDYNPLMEARRLRSALLFQHEDLIGRCHAFDGTILFLPKRLQHKVTEVFSQTRNGEHVRITITLTNELPPTSPTCLQFYNIIFRRLLKIMNLQQIGRNYYNPSDPIDIPNHRLVIWPGFTTSILQYENNIMLCTDVSHKVLRSETVLDFMFNLYQQTEEHKFQEQVSKELIGLIVLTKYNNKTYRVDDIDWDQNPKSTFKKADGSEVSFLEYYRKQYNQEITDLKQPVLVSQPKRRRGPGGTLPGPAMLIPELCYLTGLTDKMRNDFNVMKDLAVHTRLTPEQRQREVGRLIDYIHKDDNVQRELRDWGLSFDSNLLSFSGRILQSEKIHQGGKTFDYNPQFADWSKETRGAPLISVKPLDNWLLIYTRRNYEAANSLIQNLFKVTPAMGIQMKKAIMIEVDDRTEAYLRALQQKVTSDTQIVVCLLSSNRKDKYDAIKKYLCTDCPTPSQCVVARTLGKQQTVMAIATKIALQMNCKMGGELWRVDMPLKLAMIVGIDCYHDTTAGRRSIAGFVASINEGMTRWFSRCVFQDRGQELVDGLKVCLQAALRAWSGCNEYMPSRVIVYRDGVGDGQLKTLVNYEVPQFLDCLKSVGRGYNPRLTVIVVKKRVNARFFAQSGGRLQNPLPGTVIDVEVTRPEWYDFFIVSQAVRSGSVSPTHYNVIYDSSGLKPDHIQRLTYKLCHVYYNWPGVIRVPAPCQYAHKLAFLVGQSIHREPNLSLSNRLYYL DIP protein Q9JMB7 original sequence version: 95 DIP protein Q9JMB7 original sequence version: 69 mint Probable component of some RISC complex (RNA-induced silencing), which plays a role in spermatogenesis and in germ cell production, proliferation and maintenance. RISC complexes mediate RNA cleavage and translational silencing. Overexpression may cause malignant germline development. May be associated with proliferating activity of cancer cells. When suppressed, inhibits the growth of gastric cancer cells and induced cell cycle arrest in G2/M phase. Inhibits endoribonuclease DICER1 activity 45588D13284CCC4C piwil1-tdrkh 1 4 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 3 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association f1c f1d In adult testes, Tdrkh and Miwi reciprocally co-immunoprecipitated each other (Figure 1C and D), but no in vivo interaction between Mili and Tdrkh was noted (Figure 1C). Furthermore, Tdrkh and Miwi2 co-localize in pre-natal testes (Supplementary Figure 3h). Thus, Tdrkh specifically interacts with Miwi and Miwi2. piwil2-piwil1 1 2 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level 4 western blot western blot Immuno blot Immuno blot western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association f1c In adult testes, Tdrkh and Miwi reciprocally co-immunoprecipitated each other (Figure 1C and D), but no in vivo interaction between Mili and Tdrkh was noted (Figure 1C). Furthermore, Tdrkh and Miwi2 co-localize in pre-natal testes (Supplementary Figure 3h). Thus, Tdrkh specifically interacts with Miwi and Miwi2.