IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Phosphorylation of EZH2 Activates STAT3 Signaling via STAT3 Methylation and Promotes Tumorigenicity of Glioblastoma Stem-like Cells. Phosphorylation of EZH2 Activates STAT3 Signaling via STAT3 Methylation and Promotes Tumorigenicity of Glioblastoma Stem-like Cells. Cancer Cell (1535-6108) 2013 imex curation Kim E., Kim M., Woo D.H., Shin Y., Shin J., Chang N., Oh Y.T., Kim H., Rheey J., Nakano I., Lee C., Joo K.M., Rich J.N., Nam D.H., Lee J. Cancer - Interactions investigated in the context of cancer Glioblastoma multiforme Stem-like cells (GSCs) thought to be a type of Cancer stem/propagating cells (CSCs). Interactions involving V5-STAT3 and Flag-EZH2 constructs (figures 4A and 7B) have not been included, as the origins of these constructs is not given in the publication, and the authors have not replied to requests for information regarding this matter. nsnam@skku.edu,leej7@ccf.org 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human-neuronal_precursor_cells(stem cells) Human Neuronal Precursor Cells/neural stem progenitor cells neuronal_precursor (stem cells) Neuronal Precursor Cells/neural stem progenitor cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kim E., Kim M., Woo D.H., Shin Y., Shin J., Chang N., Oh Y.T., Kim H., Rheey J., Nakano I., Lee C., Joo K.M., Rich J.N., Nam D.H., Lee J. Cancer - Interactions investigated in the context of cancer Glioblastoma multiforme Stem-like cells (GSCs) thought to be a type of Cancer stem/propagating cells (CSCs). Interactions involving V5-STAT3 and Flag-EZH2 constructs (figures 4A and 7B) have not been included, as the origins of these constructs is not given in the publication, and the authors have not replied to requests for information regarding this matter. nsnam@skku.edu,leej7@ccf.org Accepted 2013-JUL-22 by ORCHARD 02-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Phosphorylation of EZH2 Activates STAT3 Signaling via STAT3 Methylation and Promotes Tumorigenicity of Glioblastoma Stem-like Cells. Phosphorylation of EZH2 Activates STAT3 Signaling via STAT3 Methylation and Promotes Tumorigenicity of Glioblastoma Stem-like Cells. Cancer Cell (1535-6108) 2013 imex curation Kim E., Kim M., Woo D.H., Shin Y., Shin J., Chang N., Oh Y.T., Kim H., Rheey J., Nakano I., Lee C., Joo K.M., Rich J.N., Nam D.H., Lee J. Cancer - Interactions investigated in the context of cancer Glioblastoma multiforme Stem-like cells (GSCs) thought to be a type of Cancer stem/propagating cells (CSCs). Interactions involving V5-STAT3 and Flag-EZH2 constructs (figures 4A and 7B) have not been included, as the origins of these constructs is not given in the publication, and the authors have not replied to requests for information regarding this matter. nsnam@skku.edu,leej7@ccf.org 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human-u251 Homo sapiens u251 U2521 glioblastoma cell line anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kim E., Kim M., Woo D.H., Shin Y., Shin J., Chang N., Oh Y.T., Kim H., Rheey J., Nakano I., Lee C., Joo K.M., Rich J.N., Nam D.H., Lee J. Cancer - Interactions investigated in the context of cancer Glioblastoma multiforme Stem-like cells (GSCs) thought to be a type of Cancer stem/propagating cells (CSCs). Interactions involving V5-STAT3 and Flag-EZH2 constructs (figures 4A and 7B) have not been included, as the origins of these constructs is not given in the publication, and the authors have not replied to requests for information regarding this matter. nsnam@skku.edu,leej7@ccf.org Accepted 2013-JUL-22 by ORCHARD 02-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Phosphorylation of EZH2 Activates STAT3 Signaling via STAT3 Methylation and Promotes Tumorigenicity of Glioblastoma Stem-like Cells. Phosphorylation of EZH2 Activates STAT3 Signaling via STAT3 Methylation and Promotes Tumorigenicity of Glioblastoma Stem-like Cells. Cancer Cell (1535-6108) 2013 imex curation Kim E., Kim M., Woo D.H., Shin Y., Shin J., Chang N., Oh Y.T., Kim H., Rheey J., Nakano I., Lee C., Joo K.M., Rich J.N., Nam D.H., Lee J. Cancer - Interactions investigated in the context of cancer Glioblastoma multiforme Stem-like cells (GSCs) thought to be a type of Cancer stem/propagating cells (CSCs). Interactions involving V5-STAT3 and Flag-EZH2 constructs (figures 4A and 7B) have not been included, as the origins of these constructs is not given in the publication, and the authors have not replied to requests for information regarding this matter. nsnam@skku.edu,leej7@ccf.org 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human-u87 Human U-87 MG glioblastoma cell line u87 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kim E., Kim M., Woo D.H., Shin Y., Shin J., Chang N., Oh Y.T., Kim H., Rheey J., Nakano I., Lee C., Joo K.M., Rich J.N., Nam D.H., Lee J. Cancer - Interactions investigated in the context of cancer Glioblastoma multiforme Stem-like cells (GSCs) thought to be a type of Cancer stem/propagating cells (CSCs). Interactions involving V5-STAT3 and Flag-EZH2 constructs (figures 4A and 7B) have not been included, as the origins of these constructs is not given in the publication, and the authors have not replied to requests for information regarding this matter. nsnam@skku.edu,leej7@ccf.org Accepted 2013-JUL-22 by ORCHARD 02-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation Phosphorylation of EZH2 Activates STAT3 Signaling via STAT3 Methylation and Promotes Tumorigenicity of Glioblastoma Stem-like Cells. Phosphorylation of EZH2 Activates STAT3 Signaling via STAT3 Methylation and Promotes Tumorigenicity of Glioblastoma Stem-like Cells. Cancer Cell (1535-6108) 2013 imex curation Kim E., Kim M., Woo D.H., Shin Y., Shin J., Chang N., Oh Y.T., Kim H., Rheey J., Nakano I., Lee C., Joo K.M., Rich J.N., Nam D.H., Lee J. Cancer - Interactions investigated in the context of cancer Glioblastoma multiforme Stem-like cells (GSCs) thought to be a type of Cancer stem/propagating cells (CSCs). Interactions involving V5-STAT3 and Flag-EZH2 constructs (figures 4A and 7B) have not been included, as the origins of these constructs is not given in the publication, and the authors have not replied to requests for information regarding this matter. nsnam@skku.edu,leej7@ccf.org 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human-csc Human Cancer Stem Cells csc Cancer Stem Cells anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kim E., Kim M., Woo D.H., Shin Y., Shin J., Chang N., Oh Y.T., Kim H., Rheey J., Nakano I., Lee C., Joo K.M., Rich J.N., Nam D.H., Lee J. Cancer - Interactions investigated in the context of cancer Glioblastoma multiforme Stem-like cells (GSCs) thought to be a type of Cancer stem/propagating cells (CSCs). Interactions involving V5-STAT3 and Flag-EZH2 constructs (figures 4A and 7B) have not been included, as the origins of these constructs is not given in the publication, and the authors have not replied to requests for information regarding this matter. nsnam@skku.edu,leej7@ccf.org Accepted 2013-JUL-22 by ORCHARD 02-Aug-2013: Contacted by IntAct-Help. Cancer Cell (1535-6108) 2013 Only protein-protein interactions imex curation suz12_human Polycomb protein SUZ12 CHET9 JJAZ1 KIAA0160 SUZ12 Suppressor of zeste 12 protein homolog Joined to JAZF1 protein Chromatin precipitated E2F target 9 protein protein protein human Homo sapiens Human MAPQKHGGGGGGGSGPSAGSGGGGFGGSAAVAAATASGGKSGGGSCGGGGSYSASSSSSAAAAAGAAVLPVKKPKMEHVQADHELFLQAFEKPTQIYRFLRTRNLIAPIFLHRTLTYMSHRNSRTNIKRKTFKVDDMLSKVEKMKGEQESHSLSAHLQLTFTGFFHKNDKPSPNSENEQNSVTLEVLLVKVCHKKRKDVSCPIRQVPTGKKQVPLNPDLNQTKPGNFPSLAVSSNEFEPSNSHMVKSYSLLFRVTRPGRREFNGMINGETNENIDVNEELPARRKRNREDGEKTFVAQMTVFDKNRRLQLLDGEYEVAMQEMEECPISKKRATWETILDGKRLPPFETFSQGPTLQFTLRWTGETNDKSTAPIAKPLATRNSESLHQENKPGSVKPTQTIAVKESLTTDLQTRKEKDTPNENRQKLRIFYQFLYNNNTRQQTEARDDLHCPWCTLNCRKLYSLLKHLKLCHSRFIFNYVYHPKGARIDVSINECYDGSYAGNPQDIHRQPGFAFSRNGPVKRTPITHILVCRPKRTKASMSEFLESEDGEVEQQRTYSSGHNRLYFHSDTCLPLRPQEMEVDSEDEKDPEWLREKTITQIEEFSDVNEGEKEVMKLWNLHVMKHGFIADNQMNHACMLFVENYGQKIIKKNLCRNFMLHLVSMHDFNLISIMSIDKAVTKLREMQQKLEKGESASPANEEITEEQNGTANGFSEINSKEKALETDSVSGVSKQSKKQKL DIP protein Q15022 original sequence version: 92 DIP protein Q15022 original sequence version: 88 DIP protein Q15022 original sequence version: 91 DIP protein Q15022 original sequence version: 106 A8830EBC3FD38D56 stat3_human Signal transducer and activator of transcription 3 Acute-phase response factor STAT3 APRF protein protein human Homo sapiens Human MAQWNQLQQLDTRYLEQLHQLYSDSFPMELRQFLAPWIESQDWAYAASKESHATLVFHNLLGEIDQQYSRFLQESNVLYQHNLRRIKQFLQSRYLEKPMEIARIVARCLWEESRLLQTAATAAQQGGQANHPTAAVVTEKQQMLEQHLQDVRKRVQDLEQKMKVVENLQDDFDFNYKTLKSQGDMQDLNGNNQSVTRQKMQQLEQMLTALDQMRRSIVSELAGLLSAMEYVQKTLTDEELADWKRRQQIACIGGPPNICLDRLENWITSLAESQLQTRQQIKKLEELQQKVSYKGDPIVQHRPMLEERIVELFRNLMKSAFVVERQPCMPMHPDRPLVIKTGVQFTTKVRLLVKFPELNYQLKIKVCIDKDSGDVAALRGSRKFNILGTNTKVMNMEESNNGSLSAEFKHLTLREQRCGNGGRANCDASLIVTEELHLITFETEVYHQGLKIDLETHSLPVVVISNICQMPNAWASILWYNMLTNNPKNVNFFTKPPIGTWDQVAEVLSWQFSSTTKRGLSIEQLTTLAEKLLGPGVNYSGCQITWAKFCKENMAGKGFSFWVWLDNIIDLVKKYILALWNEGYIMGFISKERERAILSTKPPGTFLLRFSESSKEGGVTFTWVEKDISGKTQIQSVEPYTKQQLNNMSFAEIIMGYKIMDATNILVSPLVYLYPDIPKEEAFGKYCRPESQEHPEADPGSAAPYLKTKFICVTPTTCSNTIDLPMSPRTLDSLMQFGNNGEGAEPSAGGQFESLTFDMELTSECATSPM 6C00632211C8012D DIP protein P40763 original sequence version: 157 DIP protein P40763-1 original sequence version: 147 DIP protein P40763-1 original sequence version: 135 DIP protein P40763-1 original sequence version: 157 DIP protein P40763 original sequence version: 165 DIP protein P40763 original sequence version: 182 DIP protein P40763 original sequence version: 135 6C00632211C8012D ezh2_human Histone-lysine N-methyltransferase EZH2 KMT6 Enhancer of zeste homolog 2 ENX-1 Lysine N-methyltransferase 6 EZH2 protein protein human Homo sapiens Human MGQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFSSNRQKILERTEILNQEWKQRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEKYKELTEQQLPGALPPECTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTPPKRPGGRRRGRLPNNSSRPSTPTINVLESKDTDSDREAGTETGGENNDKEEEEKKDETSSSSEANSRCQTPIKMKPNIEPPENVEWSGAEASMFRVLIGTYYDNFCAIARLIGTKTCRQVYEFRVKESSIIAPAPAEDVDTPPRKKKRKHRLWAAHCRKIQLKKDGSSNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCGAADHWDSKNVSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREMEIP DIP protein Q15910 original sequence version: 97 DIP protein Q15910 original sequence version: 94 DIP protein Q15910 original sequence version: 92 DIP protein Q15910 original sequence version: 96 DIP protein Q15910 original sequence version: 122 DIP protein Q15910 original sequence version: 100 1B5029EB9D509BE5 ezh2-stat3-2 4 5 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey association association 1A/B/D stat3-ezh2-1 4 5 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey association association 1A/B/C, 5C, Supp fig1 serum and/or growth factors LY294002 -a PI3K/AKT pathway inhibitor An association between EZH2 and STAT3 was prominent in GSCs, but lost in the (differentiated) cells cultured in the presence of serum or the absence of growth factors. stat3-ezh2-2 1 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey physical association physical association 1C An association between EZH2 and STAT3 was prominent, but lost in the (differentiated) NPC cells cultured in the presence of serum or the absence of growth factors. serum and/or growth factors ezh2-stat3-3 3 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association 1D Weak reaction ezh2-stat3-4 2 6 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait physical association physical association 1D Weak reaction