IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ 14-3-3 proteins interact with a hybrid prenyl-phosphorylation motif to inhibit G proteins. 14-3-3 proteins interact with a hybrid prenyl-phosphorylation motif to inhibit G proteins. Cell (0092-8674) 2013 imex curation Riou P., Kjær S., Garg R., Purkiss A., George R., Cain R.J., Bineva G., Reymond N., McColl B., Thompson A.J., O'Reilly N., McDonald N.Q., Parker P.J., Ridley A.J. Source of constructs obtained from the authors. Interactions identified using the non-specific 14-3-3 antibody were not captured. peter.parker@cancer.org.uk, anne.ridley@kcl.ac.uk 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Riou P., Kjær S., Garg R., Purkiss A., George R., Cain R.J., Bineva G., Reymond N., McColl B., Thompson A.J., O'Reilly N., McDonald N.Q., Parker P.J., Ridley A.J. Source of constructs obtained from the authors. Interactions identified using the non-specific 14-3-3 antibody were not captured. peter.parker@cancer.org.uk, anne.ridley@kcl.ac.uk Accepted 2013-NOV-07 by PPORRAS 22-Nov-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation 14-3-3 proteins interact with a hybrid prenyl-phosphorylation motif to inhibit G proteins. 14-3-3 proteins interact with a hybrid prenyl-phosphorylation motif to inhibit G proteins. Cell (0092-8674) 2013 imex curation Riou P., Kjær S., Garg R., Purkiss A., George R., Cain R.J., Bineva G., Reymond N., McColl B., Thompson A.J., O'Reilly N., McDonald N.Q., Parker P.J., Ridley A.J. Source of constructs obtained from the authors. Interactions identified using the non-specific 14-3-3 antibody were not captured. peter.parker@cancer.org.uk, anne.ridley@kcl.ac.uk 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 pull down pull down anti tag western anti tag western blot anti tag western Riou P., Kjær S., Garg R., Purkiss A., George R., Cain R.J., Bineva G., Reymond N., McColl B., Thompson A.J., O'Reilly N., McDonald N.Q., Parker P.J., Ridley A.J. Source of constructs obtained from the authors. Interactions identified using the non-specific 14-3-3 antibody were not captured. peter.parker@cancer.org.uk, anne.ridley@kcl.ac.uk Accepted 2013-NOV-07 by PPORRAS 22-Nov-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation 14-3-3 proteins interact with a hybrid prenyl-phosphorylation motif to inhibit G proteins. 14-3-3 proteins interact with a hybrid prenyl-phosphorylation motif to inhibit G proteins. Cell (0092-8674) 2013 imex curation Riou P., Kjær S., Garg R., Purkiss A., George R., Cain R.J., Bineva G., Reymond N., McColl B., Thompson A.J., O'Reilly N., McDonald N.Q., Parker P.J., Ridley A.J. Source of constructs obtained from the authors. Interactions identified using the non-specific 14-3-3 antibody were not captured. peter.parker@cancer.org.uk, anne.ridley@kcl.ac.uk 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Riou P., Kjær S., Garg R., Purkiss A., George R., Cain R.J., Bineva G., Reymond N., McColl B., Thompson A.J., O'Reilly N., McDonald N.Q., Parker P.J., Ridley A.J. Source of constructs obtained from the authors. Interactions identified using the non-specific 14-3-3 antibody were not captured. peter.parker@cancer.org.uk, anne.ridley@kcl.ac.uk Accepted 2013-NOV-07 by PPORRAS 22-Nov-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation 14-3-3 proteins interact with a hybrid prenyl-phosphorylation motif to inhibit G proteins. 14-3-3 proteins interact with a hybrid prenyl-phosphorylation motif to inhibit G proteins. Cell (0092-8674) 2013 imex curation Riou P., Kjær S., Garg R., Purkiss A., George R., Cain R.J., Bineva G., Reymond N., McColl B., Thompson A.J., O'Reilly N., McDonald N.Q., Parker P.J., Ridley A.J. Source of constructs obtained from the authors. Interactions identified using the non-specific 14-3-3 antibody were not captured. peter.parker@cancer.org.uk, anne.ridley@kcl.ac.uk 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Riou P., Kjær S., Garg R., Purkiss A., George R., Cain R.J., Bineva G., Reymond N., McColl B., Thompson A.J., O'Reilly N., McDonald N.Q., Parker P.J., Ridley A.J. Source of constructs obtained from the authors. Interactions identified using the non-specific 14-3-3 antibody were not captured. peter.parker@cancer.org.uk, anne.ridley@kcl.ac.uk Accepted 2013-NOV-07 by PPORRAS 22-Nov-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation 14-3-3 proteins interact with a hybrid prenyl-phosphorylation motif to inhibit G proteins. 14-3-3 proteins interact with a hybrid prenyl-phosphorylation motif to inhibit G proteins. Cell (0092-8674) 2013 imex curation Riou P., Kjær S., Garg R., Purkiss A., George R., Cain R.J., Bineva G., Reymond N., McColl B., Thompson A.J., O'Reilly N., McDonald N.Q., Parker P.J., Ridley A.J. Source of constructs obtained from the authors. Interactions identified using the non-specific 14-3-3 antibody were not captured. peter.parker@cancer.org.uk, anne.ridley@kcl.ac.uk 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Riou P., Kjær S., Garg R., Purkiss A., George R., Cain R.J., Bineva G., Reymond N., McColl B., Thompson A.J., O'Reilly N., McDonald N.Q., Parker P.J., Ridley A.J. Source of constructs obtained from the authors. Interactions identified using the non-specific 14-3-3 antibody were not captured. peter.parker@cancer.org.uk, anne.ridley@kcl.ac.uk Accepted 2013-NOV-07 by PPORRAS 22-Nov-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation 14-3-3 proteins interact with a hybrid prenyl-phosphorylation motif to inhibit G proteins. 14-3-3 proteins interact with a hybrid prenyl-phosphorylation motif to inhibit G proteins. Cell (0092-8674) 2013 imex curation Riou P., Kjær S., Garg R., Purkiss A., George R., Cain R.J., Bineva G., Reymond N., McColl B., Thompson A.J., O'Reilly N., McDonald N.Q., Parker P.J., Ridley A.J. Source of constructs obtained from the authors. Interactions identified using the non-specific 14-3-3 antibody were not captured. peter.parker@cancer.org.uk, anne.ridley@kcl.ac.uk 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions chlae-cos_7 Cercopithecus aethiops simian cells transformed with SV40 Grivet cos_7 cercopithecus aethiops, by transformation with SV40 anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Riou P., Kjær S., Garg R., Purkiss A., George R., Cain R.J., Bineva G., Reymond N., McColl B., Thompson A.J., O'Reilly N., McDonald N.Q., Parker P.J., Ridley A.J. Source of constructs obtained from the authors. Interactions identified using the non-specific 14-3-3 antibody were not captured. peter.parker@cancer.org.uk, anne.ridley@kcl.ac.uk Accepted 2013-NOV-07 by PPORRAS 22-Nov-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation 14-3-3 proteins interact with a hybrid prenyl-phosphorylation motif to inhibit G proteins. 14-3-3 proteins interact with a hybrid prenyl-phosphorylation motif to inhibit G proteins. Cell (0092-8674) 2013 imex curation Riou P., Kjær S., Garg R., Purkiss A., George R., Cain R.J., Bineva G., Reymond N., McColl B., Thompson A.J., O'Reilly N., McDonald N.Q., Parker P.J., Ridley A.J. Source of constructs obtained from the authors. Interactions identified using the non-specific 14-3-3 antibody were not captured. peter.parker@cancer.org.uk, anne.ridley@kcl.ac.uk 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Riou P., Kjær S., Garg R., Purkiss A., George R., Cain R.J., Bineva G., Reymond N., McColl B., Thompson A.J., O'Reilly N., McDonald N.Q., Parker P.J., Ridley A.J. Source of constructs obtained from the authors. Interactions identified using the non-specific 14-3-3 antibody were not captured. peter.parker@cancer.org.uk, anne.ridley@kcl.ac.uk Accepted 2013-NOV-07 by PPORRAS 22-Nov-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation 14-3-3 proteins interact with a hybrid prenyl-phosphorylation motif to inhibit G proteins. 14-3-3 proteins interact with a hybrid prenyl-phosphorylation motif to inhibit G proteins. Cell (0092-8674) 2013 imex curation Riou P., Kjær S., Garg R., Purkiss A., George R., Cain R.J., Bineva G., Reymond N., McColl B., Thompson A.J., O'Reilly N., McDonald N.Q., Parker P.J., Ridley A.J. Source of constructs obtained from the authors. Interactions identified using the non-specific 14-3-3 antibody were not captured. peter.parker@cancer.org.uk, anne.ridley@kcl.ac.uk 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Riou P., Kjær S., Garg R., Purkiss A., George R., Cain R.J., Bineva G., Reymond N., McColl B., Thompson A.J., O'Reilly N., McDonald N.Q., Parker P.J., Ridley A.J. I have assumed the same construct is used here as described in the PDB entry. Source of constructs obtained from the authors. Interactions identified using the non-specific 14-3-3 antibody were not captured. peter.parker@cancer.org.uk, anne.ridley@kcl.ac.uk Accepted 2013-NOV-07 by PPORRAS 22-Nov-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation 1433s_chlae_protein 14-3-3 protein sigma protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey Similar to P31947 An amino acid sequence which is not represented in UniProt 1433e_human 14-3-3 protein epsilon YWHAE protein protein human Homo sapiens Human MDDREDLVYQAKLAEQAERYDEMVESMKKVAGMDVELTVEERNLLSVAYKNVIGARRASWRIISSIEQKEENKGGEDKLKMIREYRQMVETELKLICCDILDVLDKHLIPAANTGESKVFYYKMKGDYHRYLAEFATGNDRKEAAENSLVAYKAASDIAMTELPPTHPIRLGLALNFSVFYYEILNSPDRACRLAKAAFDDAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDMQGDGEEQNKEALQDVEDENQ DIP protein P62258 original sequence version: 130 DIP protein P62258 original sequence version: 132 DIP protein P62258 original sequence version: 91 07817CCBD1F75B26 rap1a_human Ras-related protein Rap-1A GTP-binding protein smg p21A Ras-related protein Krev-1 C21KG G-22K RAP1A KREV1 protein protein human Homo sapiens Human MREYKLVVLGSGGVGKSALTVQFVQGIFVEKYDPTIEDSYRKQVEVDCQQCMLEILDTAGTEQFTAMRDLYMKNGQGFALVYSITAQSTFNDLQDLREQILRVKDTEDVPMILVGNKCDLEDERVVGKEQGQNLARQWCNCAFLESSAKSKINVNEIFYDLVRQINRKTPVEKKKPKKKSCLLL DIP protein P62834 original sequence version: 37 DIP protein P62834 original sequence version: 93 42C39290C98E0A92 1433g_human 14-3-3 protein gamma YWHAG Protein kinase C inhibitor protein 1 protein protein human Homo sapiens Human MVDREQLVQKARLAEQAERYDDMAAAMKNVTELNEPLSNEERNLLSVAYKNVVGARRSSWRVISSIEQKTSADGNEKKIEMVRAYREKIEKELEAVCQDVLSLLDNYLIKNCSETQYESKVFYLKMKGDYYRYLAEVATGEKRATVVESSEKAYSEAHEISKEHMQPTHPIRLGLALNYSVFYYEIQNAPEQACHLAKTAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDQQDDDGGEGNN DIP protein P61981 original sequence version: 126 DIP protein P61981 original sequence version: 135 B0D16C6DE1F4455D 1433f_human 14-3-3 protein eta YWHAH YWHA1 Protein AS1 protein protein human Homo sapiens Human MGDREQLLQRARLAEQAERYDDMASAMKAVTELNEPLSNEDRNLLSVAYKNVVGARRSSWRVISSIEQKTMADGNEKKLEKVKAYREKIEKELETVCNDVLSLLDKFLIKNCNDFQYESKVFYLKMKGDYYRYLAEVASGEKKNSVVEASEAAYKEAFEISKEQMQPTHPIRLGLALNFSVFYYEIQNAPEQACLLAKQAFDDAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDQQDEEAGEGN DIP protein Q04917 original sequence version: 166 DIP protein Q04917 original sequence version: 168 DIP protein Q04917 original sequence version: 175 D70FBC100C45D6E5 rhg05_human Rho GTPase-activating protein 5 ARHGAP5 RHOGAP5 Rho-type GTPase-activating protein 5 p190-B protein protein human Homo sapiens Human MMAKNKEPRPPSYTISIVGLSGTEKDKGNCGVGKSCLCNRFVRSKADEYYPEHTSVLSTIDFGGRVVNNDHFLYWGDIIQNSEDGVECKIHVIEQTEFIDDQTFLPHRSTNLQPYIKRAAASKLQSAEKLMYICTDQLGLEQDFEQKQMPEGKLNVDGFLLCIDVSQGCNRKFDDQLKFVNNLFVQLSKSKKPVIIAATKCDECVDHYLREVQAFASNKKNLLVVETSARFNVNIETCFTALVQMLDKTRSKPKIIPYLDAYKTQRQLVVTATDKFEKLVQTVRDYHATWKTVSNKLKNHPDYEEYINLEGTRKARNTFSKHIEQLKQEHIRKRREEYINTLPRAFNTLLPNLEEIEHLNWSEALKLMEKRADFQLCFVVLEKTPWDETDHIDKINDRRIPFDLLSTLEAEKVYQNHVQHLISEKRRVEMKEKFKKTLEKIQFISPGQPWEEVMCFVMEDEAYKYITEADSKEVYGRHQREIVEKAKEEFQEMLFEHSELFYDLDLNATPSSDKMSEIHTVLSEEPRYKALQKLAPDRESLLLKHIGFVYHPTKETCLSGQNCTDIKVEQLLASSLLQLDHGRLRLYHDSTNIDKVNLFILGKDGLAQELANEIRTQSTDDEYALDGKIYELDLRPVDAKSPYFLSQLWTAAFKPHGCFCVFNSIESLSFIGEFIGKIRTEASQIRKDKYMANLPFTLILANQRDSISKNLPILRHQGQQLANKLQCPFVDVPAGTYPRKFNETQIKQALRGVLESVKHNLDVVSPIPANKDLSEADLRIVMCAMCGDPFSVDLILSPFLDSHSCSAAQAGQNNSLMLDKIIGEKRRRIQITILSYHSSIGVRKDELVHGYILVYSAKRKASMGMLRAFLSEVQDTIPVQLVAVTDSQADFFENEAIKELMTEGEHIATEITAKFTALYSLSQYHRQTEVFTLFFSDVLEKKNMIENSYLSDNTRESTHQSEDVFLPSPRDCFPYNNYPDSDDDTEAPPPYSPIGDDVQLLPTPSDRSRYRLDLEGNEYPIHSTPNCHDHERNHKVPPPIKPKPVVPKTNVKKLDPNLLKTIEAGIGKNPRKQTSRVPLAHPEDMDPSDNYAEPIDTIFKQKGYSDEIYVVPDDSQNRIKIRNSFVNNTQGDEENGFSDRTSKSHGERRPSKYKYKSKTLFSKAKSYYRRTHSDASDDEAFTTSKTKRKGRHRGSEEDPLLSPVETWKGGIDNPAITSDQELDDKKMKKKTHKVKEDKKQKKKTKNFNPPTRRNWESNYFGMPLQDLVTAEKPIPLFVEKCVEFIEDTGLCTEGLYRVSGNKTDQDNIQKQFDQDHNINLVSMEVTVNAVAGALKAFFADLPDPLIPYSLHPELLEAAKIPDKTERLHALKEIVKKFHPVNYDVFRYVITHLNRVSQQHKINLMTADNLSICFWPTLMRPDFENREFLSTTKIHQSVVETFIQQCQFFFYNGEIVETTNIVAPPPPSNPGQLVEPMVPLQLPPPLQPQLIQPQLQTDPLGII GTPase-activating protein for Rho family members. May play a role in the reduction of the p21rasGTPase-activating potential of p120GAP mint homomint 2E01B5B1D007984D rnd3_human Rho-related GTP-binding protein RhoE RND3 ARHE RHO8 RHOE Rho family GTPase 3 Rnd3 Rho-related GTP-binding protein Rho8 Protein MemB protein protein human Homo sapiens Human MKERRASQKLSSKSIMDPNQNVKCKIVVVGDSQCGKTALLHVFAKDCFPENYVPTVFENYTASFEIDTQRIELSLWDTSGSPYYDNVRPLSYPDSDAVLICFDISRPETLDSVLKKWKGEIQEFCPNTKMLLVGCKSDLRTDVSTLVELSNHRQTPVSYDQGANMAKQIGAATYIECSALQSENSVRDIFHVATLACVNKTNKNVKRNKSQRATKRISHMPSRPELSAVATDLRKDKAKSCTVM DD2D4021CD42BACC 1433b_chlae_protein 14-3-3 protein beta/alpha protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey An amino acid sequence which is not represented in UniProt 1433s_human 14-3-3 protein sigma Stratifin Epithelial cell marker protein 1 HME1 SFN protein protein human Homo sapiens Human MERASLIQKAKLAEQAERYEDMAAFMKGAVEKGEELSCEERNLLSVAYKNVVGGQRAAWRVLSSIEQKSNEEGSEEKGPEVREYREKVETELQGVCDTVLGLLDSHLIKEAGDAESRVFYLKMKGDYYRYLAEVATGDDKKRIIDSARSAYQEAMDISKKEMPPTNPIRLGLALNFSVFHYEIANSPEEAISLAKTTFDEAMADLHTLSEDSYKDSTLIMQLLRDNLTLWTADNAGEEGGEAPQEPQS DIP protein P31947 original sequence version: 84 7F4B44E3AA59ECE6 1433t_human 14-3-3 protein theta YWHAQ 14-3-3 protein tau 14-3-3 protein T-cell Protein HS1 protein protein human Homo sapiens Human MEKTELIQKAKLAEQAERYDDMATCMKAVTEQGAELSNEERNLLSVAYKNVVGGRRSAWRVISSIEQKTDTSDKKLQLIKDYREKVESELRSICTTVLELLDKYLIANATNPESKVFYLKMKGDYFRYLAEVACGDDRKQTIDNSQGAYQEAFDISKKEMQPTHPIRLGLALNFSVFYYEILNNPELACTLAKTAFDEAIAELDTLNEDSYKDSTLIMQLLRDNLTLWTSDSAGEECDAAEGAEN DIP protein P27348 original sequence version: 164 DIP protein P27348 original sequence version: 173 175534325E9E37C4 1433g_chlae_protein 14-3-3 protein gamma protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey An amino acid sequence which is not represented in UniProt Similar to P61982 1433e_chlae_protein protein protein chlae Chlorocebus aethiops Cercopithecus aethiops Grivet Green monkey Similar to P62260 1433z_human 14-3-3 protein zeta/delta YWHAZ Protein kinase C inhibitor protein 1 protein protein human Homo sapiens Human MDKNELVQKAKLAEQAERYDDMAACMKSVTEQGAELSNEERNLLSVAYKNVVGARRSSWRVVSSIEQKTEGAEKKQQMAREYREKIETELRDICNDVLSLLEKFLIPNASQAESKVFYLKMKGDYYRYLAEVAAGDDKKGIVDQSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLSEESYKDSTLIMQLLRDNLTLWTSDTQGDEAEAGEGGEN DIP protein P63104 original sequence version: 144 D464DF2286BBFE60 rnd3_mouse Rho-related GTP-binding protein RhoE Rnd3 Arhe Rhoe Rho family GTPase 3 Rnd3 protein protein mouse Mus musculus Mouse MKERRASQKLSSKSIMDPNQNVKCKIVVVGDSQCGKTALLHVFAKDCFPENYVPTVFENYTASFEIDTQRIELSLWDTSGSPYYDNVRPLSYPDSDAVLICFDISRPETLDSVLKKWKGEIQEFCPNTKMLLVGCKSDLRTDVSTLVELSNHRQTPVSYDQGANMAKQIGAATYIECSALQSENSVRDIFHVATLACVNKTNKNVKRNKSQRATKRISHMPSRPELSAVATDLRKDKAKSCTVM DD2D4021CD42BACC 1433b_human 14-3-3 protein beta/alpha YWHAB Protein kinase C inhibitor protein 1 Protein 1054 protein protein human Homo sapiens Human MTMDKSELVQKAKLAEQAERYDDMAAAMKAVTEQGHELSNEERNLLSVAYKNVVGARRSSWRVISSIEQKTERNEKKQQMGKEYREKIEAELQDICNDVLELLDKYLIPNATQPESKVFYLKMKGDYFRYLSEVASGDNKQTTVSNSQQAYQEAFEISKKEMQPTHPIRLGLALNFSVFYYEILNSPEKACSLAKTAFDEAIAELDTLNEESYKDSTLIMQLLRDNLTLWTSENQGDEGDAGEGEN DIP protein P31946 original sequence version: 171 DIP protein P31946 original sequence version: 97 DIP protein P31946 original sequence version: 119 DIP protein P31946 original sequence version: 122 DIP protein P31946 original sequence version: 180 6BE1A9BF97468017 rnd3-1433b_chlae_p-1 6 19 unspecified role unspecified role prey prey 16 unspecified role unspecified role prey prey 22 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey 20 unspecified role unspecified role prey prey association association Supp. Table 1 rnd3-ywhaq 5 18 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 22 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. Fig. 1A, 1B staurosporine calyculin A rnd3-ywhab 5 22 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 23 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1A, Supp. Fig 1A, 1B Staurosporine calyculin A Calf intestinal phosphatase rnd3-ywhag 5 22 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 12 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. Fig. 1A, 1B calyculin A Staurosporine ywhag-rnd3 5 12 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 22 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. Fig. 1A ywhaq-rnd3 5 18 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 22 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. Fig. 1A ywhab-rnd3 5 22 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 23 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. Fig 1A rnd3-arhgap5 5 14 unspecified role unspecified role prey prey p region required to bind necessary binding region required to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 22 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P61588:p.Thr55Val mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain T V Wed May 02 17:40:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr55val' Tue Jun 30 17:13:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr55Val' physical association physical association Supp. Fig. 3B arhgap5-rnd3 5 14 unspecified role unspecified role bait bait region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined p region required to bind necessary binding region required to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 22 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. Fig. 3C Interaction not observed in presence of 14-3-3beta protein (YWHAB) rnd3-ywhab-1 5 23 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 22 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P61588:p.[Ser210Ala;Thr214Ala;Ser218Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain S A Wed May 02 17:59:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser210ala,thr214ala,ser218ala' Tue Jun 30 18:32:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser210Ala;Thr214Ala;Ser218Ala]' ser-210 (possible) opser O-phospho-L-serine (2S)-2-amino-3-(phosphonooxy)propanoic acid 2-amino-3-hydroxypropanoic acid 3-phosphate 2-azanyl-3-(phosphonooxy)propanoic acid ACT_SITE Phosphoserine intermediate MOD_RES Phosphoserine O-phospho-L-serine O-phosphonoserine O-phosphorylated L-serine O3-phosphoserine serine phosphate ester OPSer Phospho Phospho Seryl Phosphorylation phosphorylations certain certain sequence position certain certain certain sequence position certain physical association physical association Supp. Fig. 3C ywhae-rnd3 5 22 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. Fig. 1A rnd3-ywhae 5 22 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 10 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1A Supp. Fig. 1A, 1B staurosporine calyculin A rnd3-ywhaz 5 22 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 21 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1A Supp. Fig. 1A, 1B staurosporine calyculin A ywhah-rnd3 5 22 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 13 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. Fig. 1A rnd3-ywhah 5 13 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 22 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. Fig. 1A, 1B staurosporine calyculin A ywhaz-rnd3 5 21 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 22 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. Fig. 1A rnd3-ywhaz 5 21 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 22 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. Fig. 1A, 1B calyculin A Staurosporine sfn-rnd3-1 5 22 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 17 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. Fig. 1A ywhab-rnd3-1 2 23 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 22 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 5A ywhab-rnd3-2 2 23 competitor competitor competitor prey prey ser-240 (required) oglycoser O-glycosyl-L-serine OGlycoSer certain certain sequence position certain certain certain sequence position certain cys-241 (required) farnres farnesylated residue FarnRes Farnesyl Farnesylation certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 23 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 22 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 5A rnd3-ywhab-3 3 15 unspecified role unspecified role bait bait ser-240 (required) nphosres N-phosphorylated residue NPhosRes certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain cys-241 (required) farnres farnesylated residue FarnRes Farnesyl Farnesylation certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 23 unspecified role unspecified role prey prey region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 5B rnd3-ywhab-7 8 15 unspecified role unspecified role neutral component neutral component ser-240 (required) nphosres N-phosphorylated residue NPhosRes certain certain sequence position certain certain certain sequence position certain cys-241 (required) farnres farnesylated residue FarnRes Farnesyl Farnesylation certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 151 147 direct interaction direct interaction Fig. 5C Table 1, Supp. Fig. 5 Defarnesylation of Rnd3 increased the Kd to 1 uM. rnd3-ywhab-8 4 15 unspecified role unspecified role neutral component neutral component cys-241 (required) farnres farnesylated residue FarnRes Farnesyl Farnesylation certain certain sequence position certain certain certain sequence position certain ser-240 (required) nphosres N-phosphorylated residue NPhosRes certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. Table 4 2.3A Rworking = 0.24 Rfree = 0.27 P63104 decreases activity of P61587 rnd3-ywhaz-1 1 15 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cys-241 (required) farnres farnesylated residue FarnRes Farnesyl Farnesylation certain certain sequence position certain certain certain sequence position certain ser-240 (required) nphosres N-phosphorylated residue NPhosRes certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 168 165 direct interaction direct interaction Table 2 rnd3-ywhaz-2 1 15 unspecified role unspecified role bait bait cys-241 (required) ipcys isoprenylated cysteine IpCys certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ser-240 (required) nphosres N-phosphorylated residue NPhosRes certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 174 176 direct interaction direct interaction Table 2 rnd3-ywhaz-3 1 15 unspecified role unspecified role bait bait ser-240 (required) nphosres N-phosphorylated residue NPhosRes certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain cys-241 (required) sgergercys S-geranylgeranyl-L-cysteine 2-amino-3-(3,7,11,15-tetramethyl-2,6,10,14-hexadecatetraenylthio)propanoic acid LIPID S-geranylgeranyl cysteine S-geranylgeranyl-L-cysteine (2R)-2-amino-3-([(2E,6E,10Z)-12-hydroxy-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]sulfanyl)propanoic acid Geranyl-geranyl GeranylGeranyl S-geranylgeranyl SGergerCys geranylgeranylc certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 182 185 direct interaction direct interaction Table 2 rnd3-ywhaz-4 1 15 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ser-240 (required) nphosres N-phosphorylated residue NPhosRes certain certain sequence position certain certain certain sequence position certain cys-241 geranylated (required) modres protein modification ModRes certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 194 192 direct interaction direct interaction Table 2 rnd3-ywhaz-5 1 15 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ser-240 (required) nphosres N-phosphorylated residue NPhosRes certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Table 2 rnd3-ywhaz-6 3 15 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ser-240 (required) nphosres N-phosphorylated residue NPhosRes certain certain sequence position certain certain certain sequence position certain cys-241 (required) farnres farnesylated residue FarnRes Farnesyl Farnesylation certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 207 212 direct interaction direct interaction Fig. 7A rnd3-ywhaz-7 3 15 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ser-240 (required) nphosres N-phosphorylated residue NPhosRes certain certain sequence position certain certain certain sequence position certain cys-241 (required) ipcys isoprenylated cysteine IpCys certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 220 216 direct interaction direct interaction Fig. 7A rnd3-ywhaz-8 3 15 unspecified role unspecified role bait bait ser-240 (required) nphosres N-phosphorylated residue NPhosRes certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined cys-241 (required) sgergercys S-geranylgeranyl-L-cysteine 2-amino-3-(3,7,11,15-tetramethyl-2,6,10,14-hexadecatetraenylthio)propanoic acid LIPID S-geranylgeranyl cysteine S-geranylgeranyl-L-cysteine (2R)-2-amino-3-([(2E,6E,10Z)-12-hydroxy-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]sulfanyl)propanoic acid Geranyl-geranyl GeranylGeranyl S-geranylgeranyl SGergerCys geranylgeranylc certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 227 229 direct interaction direct interaction Fig. 7A rnd3-ywhaz-9 3 15 unspecified role unspecified role bait bait cys-241 geranylated (required) modres protein modification ModRes certain certain sequence position certain certain certain sequence position certain region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ser-240 (required) nphosres N-phosphorylated residue NPhosRes certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 21 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 235 239 direct interaction direct interaction Fig. 7A rap1a-ywhaz 1 11 unspecified role unspecified role bait bait region biotin tag biotin tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain cys-181 sgergercys S-geranylgeranyl-L-cysteine 2-amino-3-(3,7,11,15-tetramethyl-2,6,10,14-hexadecatetraenylthio)propanoic acid LIPID S-geranylgeranyl cysteine S-geranylgeranyl-L-cysteine (2R)-2-amino-3-([(2E,6E,10Z)-12-hydroxy-3,7,11-trimethyldodeca-2,6,10-trien-1-yl]sulfanyl)propanoic acid Geranyl-geranyl GeranylGeranyl S-geranylgeranyl SGergerCys geranylgeranylc certain certain sequence position certain certain certain sequence position certain 21 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 243 246 direct interaction direct interaction Fig. 7C Removal of the geranylgeranylated modification resulted in the Rap1A peptide having micromolar affinity for the 14-3-3 protein. ywhab-rnd3-3 7 15 unspecified role unspecified role prey prey P61587:p.[Ser7Ala;Ser11Ala;Ser210Ala;Thr214Ala;Ser218Ala;Ser222Ala;Ser240Ala] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain S A certain certain sequence position certain certain certain sequence position certain T A certain certain sequence position certain certain certain sequence position certain S A Thu Jul 07 12:07:33 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was ser7_11_210_214_218_222_240ala Wed May 02 18:42:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser7ala,ser11ala,ser210ala,thr214ala,ser218ala,ser222ala,ser240ala' Tue Jun 30 18:13:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Ser7Ala;Ser11Ala;Ser210Ala;Thr214Ala;Ser218Ala;Ser222Ala;Ser240Ala]' region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chemical synthesis Chemical synthesis (Chemical synthesis) 23 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. Fig. 1C