IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine. Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine. Cell (0092-8674) 2013 imex curation Stinson B.M., Nager A.R., Glynn S.E., Schmitz K.R., Baker T.A., Sauer R.T. Protease assays not captured as only involved an artefactual substrate. bobsauer@mit.edu 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Stinson B.M., Nager A.R., Glynn S.E., Schmitz K.R., Baker T.A., Sauer R.T. Until we can do sub-complexes, representing the 2 trimers with one mutated subunit is beyond what can reasonably be achieved. Done my best to represent it. Protease assays not captured as only involved an artefactual substrate. bobsauer@mit.edu Accepted 2013-SEP-19 by MGT 30-Sept-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine. Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine. Cell (0092-8674) 2013 imex curation Stinson B.M., Nager A.R., Glynn S.E., Schmitz K.R., Baker T.A., Sauer R.T. Protease assays not captured as only involved an artefactual substrate. bobsauer@mit.edu 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Stinson B.M., Nager A.R., Glynn S.E., Schmitz K.R., Baker T.A., Sauer R.T. Protease assays not captured as only involved an artefactual substrate. bobsauer@mit.edu Accepted 2013-SEP-19 by MGT 30-Sept-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine. Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine. Cell (0092-8674) 2013 imex curation Stinson B.M., Nager A.R., Glynn S.E., Schmitz K.R., Baker T.A., Sauer R.T. Protease assays not captured as only involved an artefactual substrate. bobsauer@mit.edu 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro fluorescence fluorescence technology fluorescence tag fluorescence tag visualisation by fluorescence tag fluorescence Stinson B.M., Nager A.R., Glynn S.E., Schmitz K.R., Baker T.A., Sauer R.T. Protease assays not captured as only involved an artefactual substrate. bobsauer@mit.edu Accepted 2013-SEP-19 by MGT 30-Sept-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine. Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine. Cell (0092-8674) 2013 imex curation Stinson B.M., Nager A.R., Glynn S.E., Schmitz K.R., Baker T.A., Sauer R.T. Protease assays not captured as only involved an artefactual substrate. bobsauer@mit.edu 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro atpase assay atpase assay predetermined predetermined participant predetermined Stinson B.M., Nager A.R., Glynn S.E., Schmitz K.R., Baker T.A., Sauer R.T. Protease assays not captured as only involved an artefactual substrate. bobsauer@mit.edu Accepted 2013-SEP-19 by MGT 30-Sept-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine. Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine. Cell (0092-8674) 2013 imex curation Stinson B.M., Nager A.R., Glynn S.E., Schmitz K.R., Baker T.A., Sauer R.T. Protease assays not captured as only involved an artefactual substrate. bobsauer@mit.edu 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro fluorescence fluorescence technology fluorescence predetermined predetermined participant predetermined Stinson B.M., Nager A.R., Glynn S.E., Schmitz K.R., Baker T.A., Sauer R.T. I think the fluorescent dye is in each of the 3 subunits in different runs, so I have not added it or the mutation you need to enable it to bind - just left it as an exp-mod comment. Protease assays not captured as only involved an artefactual substrate. bobsauer@mit.edu Accepted 2013-SEP-19 by MGT 30-Sept-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine. Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine. Cell (0092-8674) 2013 imex curation Stinson B.M., Nager A.R., Glynn S.E., Schmitz K.R., Baker T.A., Sauer R.T. Protease assays not captured as only involved an artefactual substrate. bobsauer@mit.edu 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Stinson B.M., Nager A.R., Glynn S.E., Schmitz K.R., Baker T.A., Sauer R.T. Protease assays not captured as only involved an artefactual substrate. bobsauer@mit.edu Accepted 2013-SEP-19 by MGT 30-Sept-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine. Nucleotide binding and conformational switching in the hexameric ring of a AAA+ machine. Cell (0092-8674) 2013 imex curation Stinson B.M., Nager A.R., Glynn S.E., Schmitz K.R., Baker T.A., Sauer R.T. Protease assays not captured as only involved an artefactual substrate. bobsauer@mit.edu 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro fluorescence fluorescence technology fluorescence predetermined predetermined participant predetermined Stinson B.M., Nager A.R., Glynn S.E., Schmitz K.R., Baker T.A., Sauer R.T. I think the fluorescent dye is in each of the 3 subunits in different runs, so I have not added it or the mutation you need to enable it to bind - just left it as an exp-mod comment. Protease assays not captured as only involved an artefactual substrate. bobsauer@mit.edu Accepted 2013-SEP-19 by MGT 30-Sept-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation atp ATP Adenosine 5'-triphosphate Adenosine triphosphate ADENOSINE-5'-TRIPHOSPHATE Adephos Adetol Adynol Atipi ATP Atriphos Cardenosine Fosfobion Glucobasin H4atp Myotriphos Triadenyl Triphosphaden adenosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) adenosine 5'-[gamma-thio]triphosphate Adenosine 5'-[gamma-thio]triphosphate small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) adp adenosine 5'-(trihydrogen diphosphate) (ADP) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) clpx_ecoli ATP-dependent Clp protease ATP-binding subunit ClpX clpX lopC b0438 JW0428 ATP-dependent unfoldase ClpX protein protein ecoli Escherichia coli (strain K12) MTDKRKDGSGKLLYCSFCGKSQHEVRKLIAGPSVYICDECVDLCNDIIREEIKEVAPHRERSALPTPHEIRNHLDDYVIGQEQAKKVLAVAVYNHYKRLRNGDTSNGVELGKSNILLIGPTGSGKTLLAETLARLLDVPFTMADATTLTEAGYVGEDVENIIQKLLQKCDYDVQKAQRGIVYIDEIDKISRKSDNPSITRDVSGEGVQQALLKLIEGTVAAVPPQGGRKHPQQEFLQVDTSKILFICGGAFAGLDKVISHRVETGSGIGFGATVKAKSDKASEGELLAQVEPEDLIKFGLIPEFIGRLPVVATLNELSEEALIQILKEPKNALTKQYQALFNLEGVDLEFRDEALDAIAKKAMARKTGARGLRSIVEAALLDTMYDLPSMEDVEKVVIDESVIDGQSKPLLIYGKPEAQQASGE DIP protein P0A6H1 original sequence version: 53 DIP protein P0A6H1 original sequence version: 90 DIP protein P0A6H1 original sequence version: 101 9DEF1B0786E42B6F clpx-1 2 11 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. Table 1 4.1A (4I34) 4.5A (4I5O) 5.7A (4I63) 4.5A (4I9K) Working/free 27.4/30.8 (4I34) 28.6/32.3 (4I5O) 30.7/31.5 (4I63) 32.2/35.2 (4I9K) adenosine_5___g-clpx 2 9 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. Table 1 3.8A Working/free 26.2/29.6 adp-clpx 2 10 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp. Table 1 3.7A Working/free 27.5/33.4 (4I4L) clpx-1 3 11 unspecified role unspecified role neutral component neutral component cys-76 fluorescent dye fluorescent dye label fluorescent dye certain certain sequence position certain certain certain sequence position certain region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0A6H1:p.Lys330Cys mutation mutation certain certain sequence position certain certain certain sequence position certain K C Wed May 02 18:59:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys330cys' Tue Jun 30 20:12:09 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys330Cys' cys-330 fluorescent dye fluorescent dye label fluorescent dye certain certain sequence position certain certain certain sequence position certain P0A6H1:p.Asp76Cys mutation mutation certain certain sequence position certain certain certain sequence position certain D C Wed May 02 17:28:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp76cys' Tue Jun 30 15:47:10 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp76Cys' P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 15:57:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 17:39:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain cys-330 fluorescent dye fluorescent dye label fluorescent dye certain certain sequence position certain certain certain sequence position certain cys-76 fluorescent dye fluorescent dye label fluorescent dye certain certain sequence position certain certain certain sequence position certain P0A6H1:p.Lys330Cys mutation mutation certain certain sequence position certain certain certain sequence position certain K C Wed May 02 19:45:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys330cys' Tue Jun 30 17:03:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys330Cys' P0A6H1:p.Asp76Cys mutation mutation certain certain sequence position certain certain certain sequence position certain D C Wed May 02 14:26:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asp76cys' Tue Jun 30 16:57:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asp76Cys' P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 17:27:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 20:09:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' ecoli Escherichia coli (strain K12) 26 32 direct interaction direct interaction Fig. 1D atp-clpx-1 4 11 enzyme enzyme neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 19:05:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 17:16:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 11 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 19:47:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 17:24:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' P0A6H1:p.Val78_Ile79delinsAlaAla mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain VI AA Thu Jul 07 12:16:38 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val78_ile79ala Fri Jul 22 10:52:53 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val78ala,ile79ala' Wed May 02 17:32:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_ile78-79ala_ala' Tue Jun 30 19:57:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val78_Ile79delinsAlaAla' P0A6H1:p.[Val78_Ile79delinsAlaAla;Glu185Gln] mutation disrupting rate mutation disrupting interaction rate mutation disrupting rate certain certain sequence position certain certain certain sequence position certain VI AA certain certain sequence position certain certain certain sequence position certain E Q Thu Jul 07 12:16:40 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val78_ile79ala_glu185gln Fri Jul 22 10:59:15 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val78ala,ile79ala,glu185gln' Wed May 02 14:34:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_ile78-79ala_ala,glu185gln' Tue Jun 30 18:26:03 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val78_Ile79delinsAlaAla;Glu185Gln]' ecoli Escherichia coli (strain K12) 41 44 atpase reaction atpase reaction Fig. 2D, Supp Fig. 1B, 3B Either Mg2+ or Co2+ required for function atp-clpx-2 4 11 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 15:43:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 20:24:40 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' ecoli Escherichia coli (strain K12) 8 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) atpase reaction atpase reaction Supp. Fig. 1B adenosine_5__-clpx-1 4 11 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 16:39:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 15:42:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' ecoli Escherichia coli (strain K12) 9 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 11 enzyme enzyme neutral component neutral component P0A6H1:p.Val78_Ile79delinsAlaAla mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain VI AA Thu Jul 07 12:17:30 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val78_ile79ala Fri Jul 22 11:02:05 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val78ala,ile79ala' Wed May 02 18:49:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_ile78-79ala_ala' Tue Jun 30 17:41:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val78_Ile79delinsAlaAla' P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 15:26:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 17:29:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 55 61 atpase reaction atpase reaction Fig. 2E, Supp. figure 3C adp-clpx 1 11 unspecified role unspecified role neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 20:32:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 18:52:35 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Supp. Fig. 2B adp-clpx-1 1 11 unspecified role unspecified role neutral component neutral component P0A6H1:p.Val78_Ile79delinsAlaAla mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain VI AA Thu Jul 07 12:16:03 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val78_ile79ala Fri Jul 22 11:07:55 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val78ala,ile79ala' Wed May 02 19:33:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_ile78-79ala_ala' Tue Jun 30 17:20:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val78_Ile79delinsAlaAla' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 14:46:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 18:47:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 16:12:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 18:41:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp Fig. 2A atp-clpx-3 4 8 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 11 enzyme enzyme neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 15:26:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 17:01:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) atpase reaction atpase reaction Fig.6C A dimer comprised of ClpX lacking a stretch of N-terminal sequence, was only 75% active, a glu185gln mutation made this construct totally inactive. clpx-2 6 11 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 16:47:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 17:59:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 6B, Supp. Fig. 2C Removal of a stretch of N-terminal sequence reduced this to a dimeric interaction. clpx-3 6 11 unspecified role unspecified role neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 14:45:53 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 19:01:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 15:06:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 17:55:23 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' P0A6H1:p.Val78_Ile79delinsAlaAla mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain VI AA Thu Jul 07 12:16:59 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val78_ile79ala Fri Jul 22 11:13:24 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val78ala,ile79ala' Wed May 02 18:53:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_ile78-79ala_ala' Tue Jun 30 20:15:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val78_Ile79delinsAlaAla' ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp Fig. 2C adp-clpx-2 5 11 unspecified role unspecified role neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 17:54:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 18:36:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' P0A6H1:p.Val78_Ile79delinsAlaAla mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain VI AA Thu Jul 07 12:17:17 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val78_ile79ala Fri Jul 22 11:03:27 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val78ala,ile79ala' Wed May 02 15:09:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_ile78-79ala_ala' Tue Jun 30 19:50:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val78_Ile79delinsAlaAla' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 16:10:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 16:13:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp Fig. 3F, 3G Binding to the rightmost wild-type subunit subunit was tight (Kapp 2-14 uM), binding to the leftmost wild-type subunit was weaker (Kapp 60-90 uM), and binding to the VI subunit was even weaker (Kapp 430 uM) or undetectable. atp-clpx-4 5 11 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 19:33:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 17:47:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' P0A6H1:p.Val78_Ile79delinsAlaAla mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain VI AA Thu Jul 07 12:17:01 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val78_ile79ala Fri Jul 22 11:13:33 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val78ala,ile79ala' Wed May 02 14:48:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_ile78-79ala_ala' Tue Jun 30 19:49:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val78_Ile79delinsAlaAla' ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 11 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 15:33:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 18:38:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 3C, Supp Fig. 3G Binding to the rightmost wild-type subunit subunit was tight (Kapp 2-14 uM), binding to the leftmost wild-type subunit was weaker (Kapp 60-90 uM), and binding to the VI subunit was even weaker (Kapp 430 uM) or undetectable adenosine_5__-clpx-2 5 11 enzyme enzyme neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 17:14:03 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 16:12:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' P0A6H1:p.Val78_Ile79delinsAlaAla mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain VI AA Thu Jul 07 12:06:54 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val78_ile79ala Fri Jul 22 10:51:21 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val78ala,ile79ala' Wed May 02 18:23:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_ile78-79ala_ala' Tue Jun 30 15:56:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val78_Ile79delinsAlaAla' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 11 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 17:44:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 18:33:43 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 3B, Supp fig. 3G Binding to the rightmost wild-type subunit subunit was tight (Kapp 2-14 uM), binding to the leftmost wild-type subunit was weaker (Kapp 60-90 uM), and binding to the VI subunit was even weaker (Kapp 430 uM) or undetectable adp-clpx-3 7 11 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0A6H1:p.Val78_Ile79delinsAlaAla mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain VI AA Thu Jul 07 12:17:23 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val78_ile79ala Fri Jul 22 11:13:43 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val78ala,ile79ala' Wed May 02 16:47:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_ile78-79ala_ala' Tue Jun 30 16:40:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val78_Ile79delinsAlaAla' P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 18:26:19 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 18:21:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' ecoli Escherichia coli (strain K12) 10 unspecified role unspecified role neutral component neutral component 11 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 15:01:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 19:53:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' ecoli Escherichia coli (strain K12) direct interaction direct interaction Supp Fig. 4C, Supp 4D The first nucleotide-binding events cause allosteric changes in both bound (rightmost) and unbound (leftmost) subunits of the ring, possibly altering the hinge conformations and rigidifying the domain-domain interfaces in nucleotide loadable subunits. adenosine_5__-clpx-3 7 9 unspecified role unspecified role neutral component neutral component 11 enzyme enzyme neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 14:58:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 19:21:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 enzyme enzyme neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 18:11:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 17:19:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0A6H1:p.Val78_Ile79delinsAlaAla mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain VI AA Thu Jul 07 12:16:09 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val78_ile79ala Fri Jul 22 10:49:43 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val78ala,ile79ala' Wed May 02 16:06:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_ile78-79ala_ala' Tue Jun 30 15:41:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val78_Ile79delinsAlaAla' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 4B, Supp Fig. 4D The first nucleotide-binding events cause allosteric changes in both bound (rightmost) and unbound (leftmost) subunits of the ring, possibly altering the hinge conformations and rigidifying the domain-domain interfaces in nucleotide loadable subunits. atp-clpx-5 7 11 unspecified role unspecified role neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 15:46:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 16:21:57 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 15:50:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 19:58:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0A6H1:p.Val78_Ile79delinsAlaAla mutation decreasing rate mutation decreasing interaction rate mutation decreasing rate certain certain sequence position certain certain certain sequence position certain VI AA Thu Jul 07 12:15:58 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was val78_ile79ala Fri Jul 22 11:05:37 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val78ala,ile79ala' Wed May 02 14:27:43 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val_ile78-79ala_ala' Tue Jun 30 17:27:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val78_Ile79delinsAlaAla' ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component direct interaction direct interaction Fig. 4C, Supp. Fig. 4D The first nucleotide-binding events cause allosteric changes in both bound (rightmost) and unbound (leftmost) subunits of the ring, possibly altering the hinge conformations and rigidifying the domain-domain interfaces in nucleotide loadable subunits. 10 uM cp7-CFP-ssrA was present in the assay mix. atp-clpx-6 4 11 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0A6H1:p.Lys330Cys mutation mutation certain certain sequence position certain certain certain sequence position certain K C Wed May 02 19:13:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys330cys' Tue Jun 30 15:49:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys330Cys' P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 20:22:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 17:20:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' cys-330 fluorescent dye fluorescent dye label fluorescent dye certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 11 enzyme enzyme neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 14:55:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 18:23:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) atpase reaction atpase reaction Supp Fig. 4A Binding of a Oregon Green dye to a met363cys mutated residue in one subunit did not affect enzyme function atp-clpx-7 4 8 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 11 enzyme enzyme neutral component neutral component cys-363 oregon green label oregon green label certain certain sequence position certain certain certain sequence position certain P0A6H1:p.Met363Cys mutation mutation certain certain sequence position certain certain certain sequence position certain M C Wed May 02 17:07:33 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met363cys' Tue Jun 30 17:07:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met363Cys' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 15:44:26 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 19:54:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' ecoli Escherichia coli (strain K12) 11 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 16:57:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 18:38:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' ecoli Escherichia coli (strain K12) atpase reaction atpase reaction Supp Fig. 3E Binding of a Oregon Green dye to a met363cys mutated residue in one subunit did not affect enzyme function atp-clpx-8 4 11 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 16:16:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 18:41:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' ecoli Escherichia coli (strain K12) 11 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 17:48:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 19:39:14 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' P0A6H1:p.Glu205Cys mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain E C Wed May 02 20:19:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu205cys' Tue Jun 30 17:15:22 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu205Cys' ecoli Escherichia coli (strain K12) 11 enzyme enzyme neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 18:00:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 18:49:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0A6H1:p.Thr147Cys mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain T C Wed May 02 15:39:01 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr147cys' Tue Jun 30 19:00:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr147Cys' ecoli Escherichia coli (strain K12) 8 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 174 178 atpase reaction atpase reaction Fig. 5C Either Mg2+ or Co2+ required for function atp-clpx-9 4 11 enzyme enzyme neutral component neutral component P0A6H1:p.Thr147Cys mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain T C Wed May 02 17:16:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr147cys' Tue Jun 30 16:38:34 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr147Cys' P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 17:12:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 19:32:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 11 enzyme enzyme neutral component neutral component P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 16:13:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 17:55:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0A6H1:p.Glu205Cys mutation increasing rate mutation increasing interaction rate mutation increasing rate certain certain sequence position certain certain certain sequence position certain E C Wed May 02 16:40:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu205cys' Tue Jun 30 19:08:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu205Cys' ecoli Escherichia coli (strain K12) 11 enzyme enzyme neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain P0A6H1:p.Cys169Ser mutation mutation certain certain sequence position certain certain certain sequence position certain C S Wed May 02 18:17:54 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'cys169ser' Tue Jun 30 17:12:08 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Cys169Ser' ecoli Escherichia coli (strain K12) 182 189 atpase reaction atpase reaction Fig. 5C Either Mg2+ or Co2+ required for function