IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3. Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3. Cell (0092-8674) 2013 imex curation Betschinger J., Nichols J., Dietmann S., Corrin P.D., Paddison P.J., Smith A. Source of constructs obtained from the author. austin.smith@cscr.cam.ac.uk,jb579@cam.ac.uk 09-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells ch-ip chromatin immunoprecipitation assay ch-ip nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Betschinger J., Nichols J., Dietmann S., Corrin P.D., Paddison P.J., Smith A. Source of constructs obtained from the author. austin.smith@cscr.cam.ac.uk,jb579@cam.ac.uk Accepted 2013-AUG-08 by MGT 09-Aug-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3. Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3. Cell (0092-8674) 2013 imex curation Betschinger J., Nichols J., Dietmann S., Corrin P.D., Paddison P.J., Smith A. Source of constructs obtained from the author. austin.smith@cscr.cam.ac.uk,jb579@cam.ac.uk 09-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Betschinger J., Nichols J., Dietmann S., Corrin P.D., Paddison P.J., Smith A. Source of constructs obtained from the author. austin.smith@cscr.cam.ac.uk,jb579@cam.ac.uk Accepted 2013-AUG-08 by MGT 09-Aug-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3. Exit from pluripotency is gated by intracellular redistribution of the bHLH transcription factor Tfe3. Cell (0092-8674) 2013 imex curation Betschinger J., Nichols J., Dietmann S., Corrin P.D., Paddison P.J., Smith A. Source of constructs obtained from the author. austin.smith@cscr.cam.ac.uk,jb579@cam.ac.uk 09-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-embryonic_stem cell Mus musculus embryonic stem cell es cells embryonic stem cells anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Betschinger J., Nichols J., Dietmann S., Corrin P.D., Paddison P.J., Smith A. Source of constructs obtained from the author. austin.smith@cscr.cam.ac.uk,jb579@cam.ac.uk Accepted 2013-AUG-08 by MGT 09-Aug-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation fnip1_mouse Folliculin-interacting protein 1 Fnip1 Kiaa1961 protein protein mouse Mus musculus Mouse MAPTLFQKLFSKRSGLGAPGRDARDPDCAFSWPLPEFDPSQIRLIVYQDCERRGRNVLFDSSVKRKNEDTSVSKLCNDAQVKVFGKCCQLKPGGDSSSSLDSSITLSSDGKDQCPKYQGSRCSSDANMLGEMMFGSVAMSYKGSTLKIHQIRSPPQLMLSKVFTARTGSSICGSLNTLQDSLEFINQDSNTLKADSSTVSNGLLGNIGLSQFCSPRRAFSEQGPLRLIRSASFFAVHSNPMDMPGRELNEDRDSGIARSASLSSLFITPFPSPNSSLTRSCASSYQRRWRRSQTTSLENGVFPRWSVEESFNLSDESCGPNPGIVRKKKIAIGVIFSLSKDEDENNKFNEFFFSHFPLFESHMNKLKSAIEQAMKMSRRSADASQRSLAYNRILDALTEFRTTICNLYTMPRIGEPVWLTMMSGTPEKNQLCHRFMKEFTFLMENASKNQFLPALITAVLTNHLAWVPTVMPNGQPPIKIFLEKHSSQSVGMLAKTHPYNPLWAQLGDLYGAIGSPVRLARTVVVGKRQDLVQRLLYFLTYFIRCSELQETHLLENGEDEAIVMPGTVITTTLEKGEIEESEYVLITMHRNKSSLLFKESEETRTPNCNCKYCSHPVLGQNTENVSQPEREDTQDNSKELLGISDECQKISPPDCQEENAVDVQQYRDKLRTCLDTKLETVVCTGSAPADKCVLSETCLEPREESWQNKELLDSDNHTGTAMRPTGIVVEKKPPDKNVPSAFSCEVTQTKVTFLIGDSMSPDSDTELRSQAVVDQINRHHSEPLKEDRGVADKHQESKITKDQSEDSDTQNIVSGESCELPCWSHSDPESMSLFDEYFNDDSIETRTIDDVPVKTSTDSKEYCCMLEYPKRLYTKTNKQKSELCKCIETVHQDSCNACFPQQDQRNSLSILVPHGDKESSDKKNAVGTEWDIPRNESSDSALGDSESEDTGPDIRRQAGGYCGGDQEDWTEEDEIPFPGSKLIEVSAVQPNIANFGRSLLGGYCSSYVPDFVLQGIGNDERLRQCLVSDLSHAVQHPVLDEPIAEAVCIIADMDKWTVQVASSQRRVTDNKLGKEVLVSSLVSNLLHSTLQLYKHNLSPNFCVMHLEDRLQELYFKSKMLSEYLRGQMRVHVKELGVVLGIESSDLPLLAAVASTHSPYVAQILL A03A39AC2FB53D29 err2_mouse_gene Mouse esrrb gene gene gene mouse Mus musculus Mouse klf2_mouse_gene Lung krueppel-like factor gene gene gene mouse Mus musculus Mouse flcn_mouse Folliculin Flcn Bhd Birt-Hogg-Dube syndrome protein homolog protein protein mouse Mus musculus Mouse MNAIVALCHFCELHGPRTLFCTEVLHAPLPQGAGSGDSPDQVEQAEEEEGGIQMSSRVRAHSPAEGASSESSSPGPKKSDMCEGCRSLAVGHPGYISHDKETSIKYVSHQHPNHPQLFSIVRQACVRSLSCEVCPGREGPIFFGDEQHGFVFSHTFFIKDSLARGFQRWYSIIAIMMDRIYLINSWPFLLGRIRGIISELQAKAFKVFEAEQFGCPQRAQRMNTAFTPFLHQRNGNAARSLTSLTSDDNLWACLHTSFAWLLKACGSRLTEKLLEGAPTEDTLVQMEKLADLEEESESWDNSEAEEEEKAPVTPEGAEGRELTSCPTESSFLSACGSWQPPKLTGFKSLRHMRQVLGAPSFRMLAWHVLMGNQVIWKSRDVNLVHSAFEVLRTMLPVGCVRIIPYSSQYEEAYRCNFLGLSPPVPIPAHVLASEFVVVVEVHTATRSNLHPAGCEDDQSLSKYEFVVTSGSPVAADRVGPTILNKIEAALTNQNLSVDVVDQCLICLKEEWMNKVKVLFKFTKVDSRPKEDTQKLLSVLGASEEDNVKLLKFWMTGLSKTYKSHLMSTVRSPTATESRS DD20DB9F7AFAC81C tfe3_mouse Transcription factor E3 Tfe3 Tcfe3 protein protein mouse Mus musculus Mouse MSHAAEPARDAVEASAEGPRAVFLLLEERRPAESAQLLSLNSLLPESGIVADIELENILDPDSFYELKSQPLFLRSSLPISLQATPTTPATLSASSSAGGSRTPAMSSSSSRVLLRQQLMRAQAQEQERRERREQAAAAPFPSPAPASPAISVIGVSAGGHTLSRPPPAQVPREVLKVQTHLENPTRYHLQQARRQQVKQYLSTTLGPKLASQALTPPPGPSSAQPLPAPETAHATGPTGSAPNSPMALLTIGSSSEKEIDDVIDEIISLESSYNDEMLSYLPGGTAGLQLPSTLPVSGNLLDVYSSQGVATPAITVSNSCPAELPNIKREISETEAKALLKERQKKDNHNLIERRRRFNINDRIKELGTLIPKSNDPEMRWNKGTILKASVDYIRKLQKEQQRSKDLESRQRSLEQANRSLQLRIQELELQAQIHGLPVPPNPGLLSLTTSSVSDSLKPEQLDIEEEGRPSTTFHVSGGPAQNAPPQQPPAPPSDALLDLHFPSDHLGDLGDPFHLGLEDILMEEEGMVGGLSGGALSPLRAASDPLLSSVSPAVSKASSRRSSFSMEEES DAB0487EC0F6E92D flcn-fnip1 2 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey physical association physical association Supp. Fig. 3B flcn-fnip1-1 3 4 unspecified role unspecified role prey prey 7 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 3D Interaction observed in both non-differentiated and differentiated cells. fnip1-flcn 3 4 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role prey prey physical association physical association Fig. 3D Interaction observed in both non-differentiated and differentiated cells. tfe3-err2 1 5 unspecified role unspecified role prey prey cis regulatory elements binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait association association Fig. 6A tfe3-klf2 1 8 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey cis regulatory elements binding region binding-associated region binding region undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 6A