IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structure of the mouse sex peptide pheromone ESP1 reveals a molecular basis for specific binding to the class C G-protein-coupled vomeronasal receptor. Structure of the mouse sex peptide pheromone ESP1 reveals a molecular basis for specific binding to the class C G-protein-coupled vomeronasal receptor. J. Biol. Chem. (0021-9258) 2013 imex curation Yoshinaga S., Sato T., Hirakane M., Esaki K., Hamaguchi T., Haga-Yamanaka S., Tsunoda M., Kimoto H., Shimada I., Touhara K., Terasawa H. terasawa@gpo.kumamoto-u.ac.jp 28-Oct_2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Yoshinaga S., Sato T., Hirakane M., Esaki K., Hamaguchi T., Haga-Yamanaka S., Tsunoda M., Kimoto H., Shimada I., Touhara K., Terasawa H. terasawa@gpo.kumamoto-u.ac.jp Accepted 2013-OCT-16 by ORCHARD 28-Oct_2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2013 Only protein-protein interactions imex curation esp1_mouse Exocrine gland-secreted peptide 1 Esp1 Gm6084 protein protein mouse Mus musculus Mouse MTSLPVLLFLIILLLPSMITEGRVLTQTGKEATIFADQKTNHEADLKNPDPQEVQRALARILCALGELDKLVKDQANAGQQEFKLPKDFTGRSKCRSLGRIK 1A149532653E91C5 v2116_mouse Vomeronasal type-2 receptor 116 Vmn2r116 V2rp5 Vomeronasal type-2 receptor P5 protein protein mouse Mus musculus Mouse MFTLIFLFLFLNIPLLVADFISPRCFWKMKQNEYRDRHHGTGCIFLILAIQQPVKKEYFSHILNIQTHTENHKYALALAFSIYEINRNPDLLPNMSLIFIFSADSCEWESELSLIRFGLQNSDNLPNYLCEELTKCILALTNMNWATTVTLHTILSNFLSDQLLHITYGTFHPALSDHEKFPYLHQMASDHTSLALALVSFIIHFGWNWVGLVISDSDQGIQFLSYLRREMEKYTLCFAFVNMIPLNINLYMSRAEVYYNQIMTSSTNVVIIYGDTDSTLAVSFRMWESLGIQRLWITTSQWDVSPSMKDFTFGNKYGTFAFEQHHSEISGFKHFVQTLNSVKCPDEYLVKLEWMHFNCEVSASKCKTLKNCSSNHSLKWLMVHTFDMAFIEESYYIYNAVYAFAHVLHQFTFQKFDNLPKDNGKEHNYSCKKLYSYLRKNHFINPVGDRVSMNQRDKLQEEYDIVYIWNFPQGLGLRVKIGMFSPYFPNGQQVHLSEDMLKWARGSTQVPTSMCSADCGPGSRKFRMDGMAACCFHCKPCPENEISNETNVDNCVQCPEDQYANTEQNHCIRKAVVFLSYEEPLGVALSLLSLCFSAFTTVVLGIFVKHHNTPIVKANNRTLTYLLLISLIFCFLCPLLFIGHPNSATCILQQLTFGVVFTVSLSTVLAKTITVVLAFKIIASQRMMKYFLISGAINYIIPICILIQVIVCAVWLRASPPSVDIDAHSEHGQIIIVCHKGSVNAFYCVLGYLAILAFGSFTLAFLSRNLPGAFNEAKSITFSMLVFCSVWVTFIPVYHSTKGKVMVAVEIFSTLASSAGMLGCIFVPKCYTILFRQDQNSLEMIRVKSSSNVHVS E9E246C276AF9761 gm6084-vmn2r116 1 2 unspecified role unspecified role bait bait in vitro In vitro 3 unspecified role unspecified role prey prey region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association Fig.5