MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Ubiquilin4 is an adaptor protein that recruits Ubiquilin1 to the autophagy machinery. Ubiquilin4 is an adaptor protein that recruits Ubiquilin1 to the autophagy machinery. EMBO Rep. (1469-221X) 2013 imex curation Yun Lee D., Arnott D., Brown E.J. Interactions using an antibody to LC3 (Cell Signaling Technology), have not been included as the antibody target is not defined. brown.eric@gene.com 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Yun Lee D., Arnott D., Brown E.J. Interactions using an antibody to LC3 (Cell Signaling Technology), have not been included as the antibody target is not defined. brown.eric@gene.com Accepted 2013-JUL-30 by MGT 02-Aug-2013: Contacted by IntAct-Help. EMBO Rep. (1469-221X) 2013 Only protein-protein interactions imex curation Ubiquilin4 is an adaptor protein that recruits Ubiquilin1 to the autophagy machinery. Ubiquilin4 is an adaptor protein that recruits Ubiquilin1 to the autophagy machinery. EMBO Rep. (1469-221X) 2013 imex curation Yun Lee D., Arnott D., Brown E.J. Interactions using an antibody to LC3 (Cell Signaling Technology), have not been included as the antibody target is not defined. brown.eric@gene.com 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yun Lee D., Arnott D., Brown E.J. Interactions using an antibody to LC3 (Cell Signaling Technology), have not been included as the antibody target is not defined. brown.eric@gene.com Accepted 2013-JUL-30 by MGT 02-Aug-2013: Contacted by IntAct-Help. EMBO Rep. (1469-221X) 2013 Only protein-protein interactions imex curation Ubiquilin4 is an adaptor protein that recruits Ubiquilin1 to the autophagy machinery. Ubiquilin4 is an adaptor protein that recruits Ubiquilin1 to the autophagy machinery. EMBO Rep. (1469-221X) 2013 imex curation Yun Lee D., Arnott D., Brown E.J. Interactions using an antibody to LC3 (Cell Signaling Technology), have not been included as the antibody target is not defined. brown.eric@gene.com 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Yun Lee D., Arnott D., Brown E.J. Interactions using an antibody to LC3 (Cell Signaling Technology), have not been included as the antibody target is not defined. brown.eric@gene.com Accepted 2013-JUL-30 by MGT 02-Aug-2013: Contacted by IntAct-Help. EMBO Rep. (1469-221X) 2013 Only protein-protein interactions imex curation Ubiquilin4 is an adaptor protein that recruits Ubiquilin1 to the autophagy machinery. Ubiquilin4 is an adaptor protein that recruits Ubiquilin1 to the autophagy machinery. EMBO Rep. (1469-221X) 2013 imex curation Yun Lee D., Arnott D., Brown E.J. Interactions using an antibody to LC3 (Cell Signaling Technology), have not been included as the antibody target is not defined. brown.eric@gene.com 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Yun Lee D., Arnott D., Brown E.J. Interactions using an antibody to LC3 (Cell Signaling Technology), have not been included as the antibody target is not defined. brown.eric@gene.com Accepted 2013-JUL-30 by MGT 02-Aug-2013: Contacted by IntAct-Help. EMBO Rep. (1469-221X) 2013 Only protein-protein interactions imex curation Ubiquilin4 is an adaptor protein that recruits Ubiquilin1 to the autophagy machinery. Ubiquilin4 is an adaptor protein that recruits Ubiquilin1 to the autophagy machinery. EMBO Rep. (1469-221X) 2013 imex curation Yun Lee D., Arnott D., Brown E.J. Interactions using an antibody to LC3 (Cell Signaling Technology), have not been included as the antibody target is not defined. brown.eric@gene.com 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip sequence tag sequence tag identification sequence tag Yun Lee D., Arnott D., Brown E.J. Interactions using an antibody to LC3 (Cell Signaling Technology), have not been included as the antibody target is not defined. brown.eric@gene.com Accepted 2013-JUL-30 by MGT 02-Aug-2013: Contacted by IntAct-Help. EMBO Rep. (1469-221X) 2013 Only protein-protein interactions imex curation Ubiquilin4 is an adaptor protein that recruits Ubiquilin1 to the autophagy machinery. Ubiquilin4 is an adaptor protein that recruits Ubiquilin1 to the autophagy machinery. EMBO Rep. (1469-221X) 2013 imex curation Yun Lee D., Arnott D., Brown E.J. Interactions using an antibody to LC3 (Cell Signaling Technology), have not been included as the antibody target is not defined. brown.eric@gene.com 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Yun Lee D., Arnott D., Brown E.J. Interactions using an antibody to LC3 (Cell Signaling Technology), have not been included as the antibody target is not defined. brown.eric@gene.com Accepted 2013-JUL-30 by MGT 02-Aug-2013: Contacted by IntAct-Help. EMBO Rep. (1469-221X) 2013 Only protein-protein interactions imex curation ubql1_human Ubiquilin-1 Protein linking IAP with cytoskeleton 1 UBQLN1 DA41 PLIC1 protein protein human Homo sapiens Human MAESGESGGPPGSQDSAAGAEGAGAPAAAASAEPKIMKVTVKTPKEKEEFAVPENSSVQQFKEEISKRFKSHTDQLVLIFAGKILKDQDTLSQHGIHDGLTVHLVIKTQNRPQDHSAQQTNTAGSNVTTSSTPNSNSTSGSATSNPFGLGGLGGLAGLSSLGLNTTNFSELQSQMQRQLLSNPEMMVQIMENPFVQSMLSNPDLMRQLIMANPQMQQLIQRNPEISHMLNNPDIMRQTLELARNPAMMQEMMRNQDRALSNLESIPGGYNALRRMYTDIQEPMLSAAQEQFGGNPFASLVSNTSSGEGSQPSRTENRDPLPNPWAPQTSQSSSASSGTASTVGGTTGSTASGTSGQSTTAPNLVPGVGASMFNTPGMQSLLQQITENPQLMQNMLSAPYMRSMMQSLSQNPDLAAQMMLNNPLFAGNPQLQEQMRQQLPTFLQQMQNPDTLSAMSNPRAMQALLQIQQGLQTLATEAPGLIPGFTPGLGALGSTGGSSGTNGSNATPSENTSPTAGTTEPGHQQFIQQMLQALAGVNPQLQNPEVRFQQQLEQLSAMGFLNREANLQALIATGGDINAAIERLLGSQPS 8B4756B6113B7025 mlp3a_human Microtubule-associated proteins 1A/1B light chain 3A Microtubule-associated protein 1 light chain 3 alpha MAP1A/MAP1B light chain 3 A MAP1 light chain 3-like protein 1 Autophagy-related protein LC3 A Autophagy-related ubiquitin-like modifier LC3 A MAP1LC3A protein protein human Homo sapiens Human MPSDRPFKQRRSFADRCKEVQQIRDQHPSKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMSELVKIIRRRLQLNPTQAFFLLVNQHSMVSVSTPIADIYEQEKDEDGFLYMVYASQETFGF 48C1FBE8F7892AF3 ubql4_human Ubiquilin-4 UBQLN4 C1orf6 UBIN Ataxin-1 ubiquitin-like-interacting protein A1U Ataxin-1 interacting ubiquitin-like protein CIP75 Connexin43-interacting protein of 75 kDa protein protein human Homo sapiens Human MAEPSGAETRPPIRVTVKTPKDKEEIVICDRASVKEFKEEISRRFKAQQDQLVLIFAGKILKDGDTLNQHGIKDGLTVHLVIKTPQKAQDPAAATASSPSTPDPASAPSTTPASPATPAQPSTSGSASSDAGSGSRRSSGGGPSPGAGEGSPSATASILSGFGGILGLGSLGLGSANFMELQQQMQRQLMSNPEMLSQIMENPLVQDMMSNPDLMRHMIMANPQMQQLMERNPEISHMLNNPELMRQTMELARNPAMMQEMMRNQDRALSNLESIPGGYNALRRMYTDIQEPMFSAAREQFGNNPFSSLAGNSDSSSSQPLRTENREPLPNPWSPSPPTSQAPGSGGEGTGGSGTSQVHPTVSNPFGINAASLGSGMFNSPEMQALLQQISENPQLMQNVISAPYMRSMMQTLAQNPDFAAQMMVNVPLFAGNPQLQEQLRLQLPVFLQQMQNPESLSILTNPRAMQALLQIQQGLQTLQTEAPGLVPSLGSFGISRTPAPSAGSNAGSTPEAPTSSPATPATSSPTGASSAQQQLMQQMIQLLAGSGNSQVQTPEVRFQQQLEQLNSMGFINREANLQALIATGGDINAAIERLLGSQLS E57B9FFEF90793FE ubiq_human UBIQ_HUMAN protein protein human Homo sapiens Human MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG sqstm_human Sequestosome-1 SQSTM1 ORCA OSIL Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa Ubiquitin-binding protein p62 EBI3-associated protein of 60 kDa protein protein human Homo sapiens Human MASLTVKAYLLGKEDAAREIRRFSFCCSPEPEAEAEAAAGPGPCERLLSRVAALFPALRPGGFQAHYRDEDGDLVAFSSDEELTMAMSYVKDDIFRIYIKEKKECRRDHRPPCAQEAPRNMVHPNVICDGCNGPVVGTRYKCSVCPDYDLCSVCEGKGLHRGHTKLAFPSPFGHLSEGFSHSRWLRKVKHGHFGWPGWEMGPPGNWSPRPPRAGEARPGPTAESASGPSEDPSVNFLKNVGESVAAALSPLGIEVDIDVEHGGKRSRLTPVSPESSSTEEKSSSQPSSCCSDPSKPGGNVEGATQSLAEQMRKIALESEGRPEEQMESDNCSGGDDDWTHLSSKEVDPSTGELQSLQMPESEGPSSLDPSQEGPTGLKEAALYPHLPPEADPRLIESLSQMLSMGFSDEGGWLTRLLQTKNYDIGAALDTIQYSKHPPPL DIP protein Q13501 original sequence version: 139 DIP protein Q13501 original sequence version: 142 DIP protein Q13501 original sequence version: 163 DIP protein Q13501 original sequence version: 146 DIP protein Q13501 original sequence version: 162 DIP protein Q13501 original sequence version: 155 DIP protein Q13501 original sequence version: 140 462D94C171F337CD ubqln1-ubiq_human-1 5 Ubqln1 7 unspecified role unspecified role bait bait n terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n terminus s tag s tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Ubql4 9 unspecified role unspecified role prey prey 10 unspecified role unspecified role prey prey association association 1a and Supp. figure 1 ubqln1-ubqln4-1 1 Ubql4 9 unspecified role unspecified role prey prey over-expressed over expressed level over-expressed region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Ubqln1 7 unspecified role unspecified role bait bait over-expressed over expressed level over-expressed n terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association figure 1b, figure 4a, figure 4b Deletion of the UBL domain in Ubqln1 had no effect on its interaction with Ubqln4; by contrast, deletion of the UBA domain decreased its association with Ubqln4 (Fig 4A,B). ubqln1-ubqln4-2 2 Ubqln1 7 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level Ubql4 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association F1C ubqln1-ubqln4-3 6 Ubqln1 7 unspecified role unspecified role neutral component neutral component n terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Ubql4 9 unspecified role unspecified role neutral component neutral component n terminus rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range Compared with wild-type Ubqln4, expression of DUBL Ubqln4 only induced about half the number of Ubqln1 puncta (Fig 3E,F) colocalization colocalization 1d, 3e, 3f Deletion of the UBA domain of Ubqln4 decreased interaction with Ubqln1. map1lc3a-ubqln4-2 6 LC3 8 unspecified role unspecified role neutral component neutral component region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Ubql4 9 unspecified role unspecified role neutral component neutral component n_terminus rfp tag red fluorescent protein tag red fluorescent protein RFP red fluorescent protein RFP rfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization 2a map1lc3a-ubqln4-1 4 Ubql4 9 unspecified role unspecified role prey prey purified purified region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined LC3 8 unspecified role unspecified role bait bait purified purified direct interaction direct interaction 2D map1lc3a-sqstm1-2 4 p62 11 unspecified role unspecified role prey prey purified purified c_terminus his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) LC3 8 unspecified role unspecified role bait bait purified purified direct interaction direct interaction 2D ubqln1-ubqln4-4 1 Ubqln1 7 unspecified role unspecified role bait bait living cell living cell n terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag predetermined featur predetermined feature predetermined featur n-term range n-terminal range n-term range n-term range n-terminal range n-term range Ubql4 9 unspecified role unspecified role prey prey living cell living cell Q9NRR5:p.Ile55Ala mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I A Wed May 02 19:19:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile55ala' Tue Jun 30 17:23:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile55Ala' region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association 4c, 4d , 4e Deletion of the UBA and UBL domains of Ubqln4 also decreased interaction with Ubqln1. ubqln4-map1lc3a 1 LC3 8 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Ubql4 9 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Supp. 3a ubqln1-ubqln4-5 3 9 unspecified role unspecified role prey prey physiological level physiological level endogenous level endogenous endogenous endogenous level region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag predetermined featur predetermined feature predetermined featur undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait physiological level physiological level endogenous level endogenous endogenous endogenous level n_terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range n_terminus s tag s tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Supp. 5b ubqln4-ubqln4-1 1 9 unspecified role unspecified role prey prey living cell living cell region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role bait bait living cell living cell n terminus gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Supp 6a