UniProt UniProt http://www.uniprot.org An unusual arrangement of two 14-3-3-like domains in the SMG5-SMG7 heterodimer is required for efficient nonsense-mediated mRNA decay. An unusual arrangement of two 14-3-3-like domains in the SMG5-SMG7 heterodimer is required for efficient nonsense-mediated mRNA decay. Genes Dev. (0890-9369) 2013 imex curation Jonas S., Weichenrieder O., Izaurralde E. http://www.gen esdev.org/cgi/doi /10.1101/gad.206672.112. Figure 6 - I have not annotated interactions with SMG-7 14-3-3 - like domain mutations because the author has not indicated precisely what these mutations are. It maybe that he is referring to mutations G100E and G103E however this has not been stated in the paper or supp materials. Figure 3B. elisa.izaurralde@tuebingen.mpg.de 29-Jun-2015: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. pull down pull down western blot western blot Immuno blot Immuno blot Jonas S., Weichenrieder O., Izaurralde E. http://www.gen esdev.org/cgi/doi /10.1101/gad.206672.112. Figure 6 - I have not annotated interactions with SMG-7 14-3-3 - like domain mutations because the author has not indicated precisely what these mutations are. It maybe that he is referring to mutations G100E and G103E however this has not been stated in the paper or supp materials. Figure 3B. elisa.izaurralde@tuebingen.mpg.de Accepted 2015-JUN-25 by ORCHARD Genes Dev. (0890-9369) 2013 Only protein-protein interactions imex curation An unusual arrangement of two 14-3-3-like domains in the SMG5-SMG7 heterodimer is required for efficient nonsense-mediated mRNA decay. An unusual arrangement of two 14-3-3-like domains in the SMG5-SMG7 heterodimer is required for efficient nonsense-mediated mRNA decay. Genes Dev. (0890-9369) 2013 imex curation Jonas S., Weichenrieder O., Izaurralde E. http://www.gen esdev.org/cgi/doi /10.1101/gad.206672.112. Figure 6 - I have not annotated interactions with SMG-7 14-3-3 - like domain mutations because the author has not indicated precisely what these mutations are. It maybe that he is referring to mutations G100E and G103E however this has not been stated in the paper or supp materials. Figure 3B. elisa.izaurralde@tuebingen.mpg.de 29-Jun-2015: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Jonas S., Weichenrieder O., Izaurralde E. http://www.gen esdev.org/cgi/doi /10.1101/gad.206672.112. Figure 6 - I have not annotated interactions with SMG-7 14-3-3 - like domain mutations because the author has not indicated precisely what these mutations are. It maybe that he is referring to mutations G100E and G103E however this has not been stated in the paper or supp materials. Figure 3B. elisa.izaurralde@tuebingen.mpg.de Accepted 2015-JUN-25 by ORCHARD Genes Dev. (0890-9369) 2013 Only protein-protein interactions imex curation An unusual arrangement of two 14-3-3-like domains in the SMG5-SMG7 heterodimer is required for efficient nonsense-mediated mRNA decay. An unusual arrangement of two 14-3-3-like domains in the SMG5-SMG7 heterodimer is required for efficient nonsense-mediated mRNA decay. Genes Dev. (0890-9369) 2013 imex curation Jonas S., Weichenrieder O., Izaurralde E. http://www.gen esdev.org/cgi/doi /10.1101/gad.206672.112. Figure 6 - I have not annotated interactions with SMG-7 14-3-3 - like domain mutations because the author has not indicated precisely what these mutations are. It maybe that he is referring to mutations G100E and G103E however this has not been stated in the paper or supp materials. Figure 3B. elisa.izaurralde@tuebingen.mpg.de 29-Jun-2015: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. pull down pull down anti tag western anti tag western blot anti tag western Jonas S., Weichenrieder O., Izaurralde E. http://www.gen esdev.org/cgi/doi /10.1101/gad.206672.112. Figure 6 - I have not annotated interactions with SMG-7 14-3-3 - like domain mutations because the author has not indicated precisely what these mutations are. It maybe that he is referring to mutations G100E and G103E however this has not been stated in the paper or supp materials. Figure 3B. elisa.izaurralde@tuebingen.mpg.de Accepted 2015-JUN-25 by ORCHARD Genes Dev. (0890-9369) 2013 Only protein-protein interactions imex curation An unusual arrangement of two 14-3-3-like domains in the SMG5-SMG7 heterodimer is required for efficient nonsense-mediated mRNA decay. An unusual arrangement of two 14-3-3-like domains in the SMG5-SMG7 heterodimer is required for efficient nonsense-mediated mRNA decay. Genes Dev. (0890-9369) 2013 imex curation Jonas S., Weichenrieder O., Izaurralde E. http://www.gen esdev.org/cgi/doi /10.1101/gad.206672.112. Figure 6 - I have not annotated interactions with SMG-7 14-3-3 - like domain mutations because the author has not indicated precisely what these mutations are. It maybe that he is referring to mutations G100E and G103E however this has not been stated in the paper or supp materials. Figure 3B. elisa.izaurralde@tuebingen.mpg.de 29-Jun-2015: Contacted by IntAct-Help. Only protein-protein interactions ecoli Escherichia coli (strain K12) pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Jonas S., Weichenrieder O., Izaurralde E. http://www.gen esdev.org/cgi/doi /10.1101/gad.206672.112. Figure 6 - I have not annotated interactions with SMG-7 14-3-3 - like domain mutations because the author has not indicated precisely what these mutations are. It maybe that he is referring to mutations G100E and G103E however this has not been stated in the paper or supp materials. Figure 3B. elisa.izaurralde@tuebingen.mpg.de Accepted 2015-JUN-25 by ORCHARD Genes Dev. (0890-9369) 2013 Only protein-protein interactions imex curation smg7_human Protein SMG7 SMG-7 homolog EST1-like protein C SMG7 KIAA0250 C1orf16 EST1C protein protein human Homo sapiens Human MSLQSAQYLRQAEVLKADMTDSKLGPAEVWTSRQALQDLYQKMLVTDLEYALDKKVEQDLWNHAFKNQITTLQGQAKNRANPNRSEVQANLSLFLEAASGFYTQLLQELCTVFNVDLPCRVKSSQLGIISNKQTHTSAIVKPQSSSCSYICQHCLVHLGDIARYRNQTSQAESYYRHAAQLVPSNGQPYNQLAILASSKGDHLTTIFYYCRSIAVKFPFPAASTNLQKALSKALESRDEVKTKWGVSDFIKAFIKFHGHVYLSKSLEKLSPLREKLEEQFKRLLFQKAFNSQQLVHVTVINLFQLHHLRDFSNETEQHTYSQDEQLCWTQLLALFMSFLGILCKCPLQNESQEESYNAYPLPAVKVSMDWLRLRPRVFQEAVVDERQYIWPWLISLLNSFHPHEEDLSSISATPLPEEFELQGFLALRPSFRNLDFSKGHQGITGDKEGQQRRIRQQRLISIGKWIADNQPRLIQCENEVGKLLFITEIPELILEDPSEAKENLILQETSVIESLAADGSPGLKSVLSTSRNLSNNCDTGEKPVVTFKENIKTREVNRDQGRSFPPKEVRRDYSKGITVTKNDGKKDNNKRKTETKKCTLEKLQETGKQNVAVQVKSQTELRKTPVSEARKTPVTQTPTQASNSQFIPIHHPGAFPPLPSRPGFPPPTYVIPPPVAFSMGSGYTFPAGVSVPGTFLQPTAHSPAGNQVQAGKQSHIPYSQQRPSGPGPMNQGPQQSQPPSQQPLTSLPAQPTAQSTSQLQVQALTQQQQSPTKAVPALGKSPPHHSGFQQYQQADASKQLWNPPQVQGPLGKIMPVKQPYYLQTQDPIKLFEPSLQPPVMQQQPLEKKMKPFPMEPYNHNPSEVKVPEFYWDSSYSMADNRSVMAQQANIDRRGKRSPGVFRPEQDPVPRMPFEKSLLEKPSELMSHSSSFLSLTGFSLNQERYPNNSMFNEVYGKNLTSSSKAELSPSMAPQETSLYSLFEGTPWSPSLPASSDHSTPASQSPHSSNPSSLPSSPPTHNHNSVPFSNFGPIGTPDNRDRRTADRWKTDKPAMGGFGIDYLSATSSSESSWHQASTPSGTWTGHGPSMEDSSAVLMESLKSIWSSSMMHPGPSALEQLLMQQKQKQQRGQGTMNPPH E152AFAFEF8F3A42 g5ef47_caeel smg-7 CELE_Y73B6A.4 Y73B6A.4 protein protein caeel Caenorhabditis elegans MSDEWEQLTVELRKIPRGTEAAPQYLRHLMKMFVADFETAVSKRFDVKFWNKLKSMMDEITKAMENDRLVNHNVQNLAIGFLTDLSLLVHYHYEIPNYGNDISKQLTWTPDVFLNRKPIKSKKNSRVFMAYVLLRMGDLMRYKENYPKAQEYYEQSCRINPADGAVWNQLGLISSLGAKNLESVYFHTRALHATMEFPTASGGLTNIFKNFANRDISRPMPIKDLYLSCLGRIHFLLEIEDSSVHLQKIGEEAATSKEMIVPLMSVYKHLEDGTELEQRAVEYVKTIWCTAYRSLLKTLDDYKEESKKLADVPHLLHILALLLCAPKLLRGIEDQTEDEVTSICEWLLCANCDEKIKDSDAFGYFHCLQRIQYPLTRTQLAQKLVEIEDEDESKDSDQSTIPEEDQKSLSTDTSRIQNEDSEPESESESSDEEVLQRGRRRGRNPNLDESVETDEDEF AD7B83B041B80E47 smg5_human Protein SMG5 SMG5 EST1B KIAA1089 EST1-like protein B LPTS-RP1 LPTS-interacting protein SMG-5 homolog protein protein human Homo sapiens Human MSQGPPTGESSEPEAKVLHTKRLYRAVVEAVHRLDLILCNKTAYQEVFKPENISLRNKLRELCVKLMFLHPVDYGRKAEELLWRKVYYEVIQLIKTNKKHIHSRSTLECAYRTHLVAGIGFYQHLLLYIQSHYQLELQCCIDWTHVTDPLIGCKKPVSASGKEMDWAQMACHRCLVYLGDLSRYQNELAGVDTELLAERFYYQALSVAPQIGMPFNQLGTLAGSKYYNVEAMYCYLRCIQSEVSFEGAYGNLKRLYDKAAKMYHQLKKCETRKLSPGKKRCKDIKRLLVNFMYLQSLLQPKSSSVDSELTSLCQSVLEDFNLCLFYLPSSPNLSLASEDEEEYESGYAFLPDLLIFQMVIICLMCVHSLERAGSKQYSAAIAFTLALFSHLVNHVNIRLQAELEEGENPVPAFQSDGTDEPESKEPVEKEEEPDPEPPPVTPQVGEGRKSRKFSRLSCLRRRRHPPKVGDDSDLSEGFESDSSHDSARASEGSDSGSDKSLEGGGTAFDAETDSEMNSQESRSDLEDMEEEEGTRSPTLEPPRGRSEAPDSLNGPLGPSEASIASNLQAMSTQMFQTKRCFRLAPTFSNLLLQPTTNPHTSASHRPCVNGDVDKPSEPASEEGSESEGSESSGRSCRNERSIQEKLQVLMAEGLLPAVKVFLDWLRTNPDLIIVCAQSSQSLWNRLSVLLNLLPAAGELQESGLALCPEVQDLLEGCELPDLPSSLLLPEDMALRNLPPLRAAHRRFNFDTDRPLLSTLEESVVRICCIRSFGHFIARLQGSILQFNPEVGIFVSIAQSEQESLLQQAQAQFRMAQEEARRNRLMRDMAQLRLQLEVSQLEGSLQQPKAQSAMSPYLVPDTQALCHHLPVIRQLATSGRFIVIIPRTVIDGLDLLKKEHPGARDGIRYLEAEFKKGNRYIRCQKEVGKSFERHKLKRQDADAWTLYKILDSCKQLTLAQGAGEEDPSGMVTIITGLPLDNPSVLSGPMQAALQAAAHASVDIKNVLDFYKQWKEIG B6F8F9B53CF42195 g5ecf1_caeel smg-5 CELE_W02D3.8 W02D3.8 protein protein caeel Caenorhabditis elegans MQKSDEVTEKFKRYCNQLEKYGQTENVHSPVMAMLRRKGRKQLIEIMKRDGDCTSSINKLWIVGYYHPFQFFIRDKEKNMAIAVLLTMFCGELQEMLSLPDDKYPALWNMYIGDFHRYMPDEEIQKCLAVGYYSRAIDLDPNQGRAFHVLAGLRADLNVAQKLRLMILGQLADAPYKKGTELLEYLKFPQKESTDKLMVDFVIWALNEKSKRMDYQMTGIKIVNEFKAEIEQKLEFDWSLIMSTCRLASKLAMKKFGFQQFYNCFDTISTLYITIYSRTISSKCLLAEAISWISDSAEILGHLDEQKNEPHFQKLSVFAKTKWNELNDLVMNHINSVFTSMSLTINPSISMTSFLLNGPISEPNVEFLSQLINYLVSVEFPPMEIIHDREESGPLLRRINQSEQKRLDIQIKTQNDEVNREDWRPVYVLMDYDVIVDKIRIALKIWDIDDFICILPSTVLDELDYQKTKNRAVRPVIRALMELQAEGKIVLKKCDNERSCAEQLVQSARRSAEDHKHIVAFLCKNPSEQKEMEGVTFYDIDQFYMKYLE 304B9C6C05A3EC08 rent1_human Regulator of nonsense transcripts 1 UPF1 RENT1 KIAA0221 ATP-dependent helicase RENT1 Nonsense mRNA reducing factor 1 Up-frameshift suppressor 1 homolog protein protein human Homo sapiens Human MSVEAYGPSSQTLTFLDTEEAELLGADTQGSEFEFTDFTLPSQTQTPPGGPGGPGGGGAGGPGGAGAGAAAGQLDAQVGPEGILQNGAVDDSVAKTSQLLAELNFEEDEEDTYYTKDLPIHACSYCGIHDPACVVYCNTSKKWFCNGRGNTSGSHIVNHLVRAKCKEVTLHKDGPLGETVLECYNCGCRNVFLLGFIPAKADSVVVLLCRQPCASQSSLKDINWDSSQWQPLIQDRCFLSWLVKIPSEQEQLRARQITAQQINKLEELWKENPSATLEDLEKPGVDEEPQHVLLRYEDAYQYQNIFGPLVKLEADYDKKLKESQTQDNITVRWDLGLNKKRIAYFTLPKTDSGNEDLVIIWLRDMRLMQGDEICLRYKGDLAPLWKGIGHVIKVPDNYGDEIAIELRSSVGAPVEVTHNFQVDFVWKSTSFDRMQSALKTFAVDETSVSGYIYHKLLGHEVEDVIIKCQLPKRFTAQGLPDLNHSQVYAVKTVLQRPLSLIQGPPGTGKTVTSATIVYHLARQGNGPVLVCAPSNIAVDQLTEKIHQTGLKVVRLCAKSREAIDSPVSFLALHNQIRNMDSMPELQKLQQLKDETGELSSADEKRYRALKRTAERELLMNADVICCTCVGAGDPRLAKMQFRSILIDESTQATEPECMVPVVLGAKQLILVGDHCQLGPVVMCKKAAKAGLSQSLFERLVVLGIRPIRLQVQYRMHPALSAFPSNIFYEGSLQNGVTAADRVKKGFDFQWPQPDKPMFFYVTQGQEEIASSGTSYLNRTEAANVEKITTKLLKAGAKPDQIGIITPYEGQRSYLVQYMQFSGSLHTKLYQEVEIASVDAFQGREKDFIILSCVRANEHQGIGFLNDPRRLNVALTRARYGVIIVGNPKALSKQPLWNHLLNYYKEQKVLVEGPLNNLRESLMQFSKPRKLVNTINPGARFMTTAMYDAREAIIPGSVYDRSSQGRPSSMYFQTHDQIGMISAGPSHVAAMNIPIPFNLVMPPMPPPGYFGQANGPAAGRGTPKGKTGRGGRQKNRFGLPGPSQTNLPNSQASQDVASQPFSQGALTQGYISMSQPSQMSQPGLSQPELSQDSYLGDEFKSQIDVALSQDSTYQGERAYQHGGVTGLSQY DIP protein Q92900 original sequence version: 137 DIP protein Q92900 original sequence version: 76 DIP protein Q92900 original sequence version: 101 DIP protein Q92900 original sequence version: 102 DIP protein Q92900 original sequence version: 135 DIP protein Q92900-1 original sequence version: 123 DIP protein Q92900 original sequence version: 132 6CCA6FE42B15BA28 smg_5-smg_7-4 4 8 unspecified role unspecified role neutral component neutral component gst gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag exp feature detect experimental feature detection exp feature detect n-term range n-terminal range n-term range n-term range n-terminal range n-term range G5ECF1:p.Gly91Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G E Wed May 02 19:43:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly91glu' Tue Jun 30 18:43:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly91Glu' ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component region mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag exp feature detect experimental feature detection exp feature detect undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined G5EF47:p.Gly80Glu mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain G E Wed May 02 14:41:40 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly80glu' Tue Jun 30 18:56:53 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly80Glu' ecoli Escherichia coli (strain K12) physical association physical association 3A Rworking 0.222 Rfree 0.249 Fig. 3a smg_5-smg_7-1 3 6 unspecified role unspecified role prey prey g80 required to bind necessary binding region required to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain v5 v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait q94 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ha ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined h116 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain g91 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Figure 3B. smg5-smg7-2 3 5 unspecified role unspecified role prey prey Q92540:p.Gly100Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G E Wed May 02 17:13:28 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly100glu' Tue Jun 30 20:11:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly100Glu' Q92540:p.Thr103Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain T E Wed May 02 19:19:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr103glu' Tue Jun 30 18:45:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr103Glu' ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait gly120glu required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain G E v5 tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sbp sbp undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 3C, 6B smg5-smg7-1 3 5 unspecified role unspecified role prey prey yfp yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 7 unspecified role unspecified role bait bait Q9UPR3:p.Gly120Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain G E Wed May 02 20:01:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly120glu' Tue Jun 30 15:49:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly120Glu' v5 tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sbp sbp undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Figure 6A. smg5-upf1-1 1 5 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey region nphosres N-phosphorylated residue NPhosRes undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined observed-ptm observed-ptm observed ptm observed-ptm 7 unspecified role unspecified role bait bait v5 tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sbp sbp undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Figure 6B smg7-upf1-1 3 5 unspecified role unspecified role bait bait k54 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain r141 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain y142 required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain v5 tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role prey prey ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association Figure 6A. smg7-smg5-1 1 7 unspecified role unspecified role prey prey ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 9 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait v5 tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sbp sbp undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q92540:p.Arg163Glu mutation mutation certain certain sequence position certain certain certain sequence position certain R E Wed May 02 16:41:25 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg163glu' Tue Jun 30 17:34:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg163Glu' Q92540:p.Lys66Glu mutation mutation certain certain sequence position certain certain certain sequence position certain K E Wed May 02 18:27:51 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys66glu' Tue Jun 30 16:51:18 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys66Glu' association association Figure 6C. upf1-smg5-3 1 7 unspecified role unspecified role prey prey 9 unspecified role unspecified role bait bait region v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region sbp sbp undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role prey prey association association Figure 6D smg5-smg7-3 3 5 unspecified role unspecified role prey prey region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 9 unspecified role unspecified role prey prey ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 7 unspecified role unspecified role bait bait v5 tag v5 tag v5 tag GKPIPNPLLGLDST epitope tag GKPIPNPLLGLDST epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sbp sbp undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Figure 6A smg_5-smg_7-5 2 8 unspecified role unspecified role neutral component neutral component gst gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag exp feature detect experimental feature detection exp feature detect n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 6 unspecified role unspecified role neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag exp feature detect experimental feature detection exp feature detect undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) direct interaction direct interaction 3A Rworking 0.222 Rfree 0.249 Supp. Table 1