IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The myosin chaperone UNC-45 is organized in tandem modules to support myofilament formation in C. elegans. The myosin chaperone UNC-45 is organized in tandem modules to support myofilament formation in C. elegans. Cell (0092-8674) 2013 imex curation Gazda L., Pokrzywa W., Hellerschmied D., Löwe T., Forné I., Mueller-Planitz F., Hoppe T., Clausen T. thorsten.hoppe@uni-koeln.de, clausen@imp.univie.ac.at 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Gazda L., Pokrzywa W., Hellerschmied D., Löwe T., Forné I., Mueller-Planitz F., Hoppe T., Clausen T. Proteins containing the UV-inducible crosslinking amino acid p-benzoyl-L-phenylalanine were UV-irradiated for 15-30 minutes thorsten.hoppe@uni-koeln.de, clausen@imp.univie.ac.at Accepted 2013-JUN-27 by PPORRAS 02-Aug-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation The myosin chaperone UNC-45 is organized in tandem modules to support myofilament formation in C. elegans. The myosin chaperone UNC-45 is organized in tandem modules to support myofilament formation in C. elegans. Cell (0092-8674) 2013 imex curation Gazda L., Pokrzywa W., Hellerschmied D., Löwe T., Forné I., Mueller-Planitz F., Hoppe T., Clausen T. thorsten.hoppe@uni-koeln.de, clausen@imp.univie.ac.at 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions caeel Caenorhabditis elegans anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Gazda L., Pokrzywa W., Hellerschmied D., Löwe T., Forné I., Mueller-Planitz F., Hoppe T., Clausen T. thorsten.hoppe@uni-koeln.de, clausen@imp.univie.ac.at Accepted 2013-JUN-27 by PPORRAS 02-Aug-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation The myosin chaperone UNC-45 is organized in tandem modules to support myofilament formation in C. elegans. The myosin chaperone UNC-45 is organized in tandem modules to support myofilament formation in C. elegans. Cell (0092-8674) 2013 imex curation Gazda L., Pokrzywa W., Hellerschmied D., Löwe T., Forné I., Mueller-Planitz F., Hoppe T., Clausen T. thorsten.hoppe@uni-koeln.de, clausen@imp.univie.ac.at 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink weight by comassie molecular weight estimation by coomasie staining weight by comassie Gazda L., Pokrzywa W., Hellerschmied D., Löwe T., Forné I., Mueller-Planitz F., Hoppe T., Clausen T. Proteins containing the UV-inducible crosslinking amino acid p-benzoyl-L-phenylalanine were UV-irradiated for 15-30 minutes. Protein complexes separated by SEC. thorsten.hoppe@uni-koeln.de, clausen@imp.univie.ac.at Accepted 2013-JUN-27 by PPORRAS 02-Aug-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation The myosin chaperone UNC-45 is organized in tandem modules to support myofilament formation in C. elegans. The myosin chaperone UNC-45 is organized in tandem modules to support myofilament formation in C. elegans. Cell (0092-8674) 2013 imex curation Gazda L., Pokrzywa W., Hellerschmied D., Löwe T., Forné I., Mueller-Planitz F., Hoppe T., Clausen T. thorsten.hoppe@uni-koeln.de, clausen@imp.univie.ac.at 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Gazda L., Pokrzywa W., Hellerschmied D., Löwe T., Forné I., Mueller-Planitz F., Hoppe T., Clausen T. thorsten.hoppe@uni-koeln.de, clausen@imp.univie.ac.at Accepted 2013-JUN-27 by PPORRAS 02-Aug-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation The myosin chaperone UNC-45 is organized in tandem modules to support myofilament formation in C. elegans. The myosin chaperone UNC-45 is organized in tandem modules to support myofilament formation in C. elegans. Cell (0092-8674) 2013 imex curation Gazda L., Pokrzywa W., Hellerschmied D., Löwe T., Forné I., Mueller-Planitz F., Hoppe T., Clausen T. thorsten.hoppe@uni-koeln.de, clausen@imp.univie.ac.at 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Gazda L., Pokrzywa W., Hellerschmied D., Löwe T., Forné I., Mueller-Planitz F., Hoppe T., Clausen T. thorsten.hoppe@uni-koeln.de, clausen@imp.univie.ac.at Accepted 2013-JUN-27 by PPORRAS 02-Aug-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation The myosin chaperone UNC-45 is organized in tandem modules to support myofilament formation in C. elegans. The myosin chaperone UNC-45 is organized in tandem modules to support myofilament formation in C. elegans. Cell (0092-8674) 2013 imex curation Gazda L., Pokrzywa W., Hellerschmied D., Löwe T., Forné I., Mueller-Planitz F., Hoppe T., Clausen T. thorsten.hoppe@uni-koeln.de, clausen@imp.univie.ac.at 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro crosslink cross-linking study crosslink sequence tag sequence tag identification sequence tag Gazda L., Pokrzywa W., Hellerschmied D., Löwe T., Forné I., Mueller-Planitz F., Hoppe T., Clausen T. Proteins containing the UV-inducible crosslinking amino acid p-benzoyl-L-phenylalanine were UV-irradiated for 15-30 minutes thorsten.hoppe@uni-koeln.de, clausen@imp.univie.ac.at Accepted 2013-JUN-27 by PPORRAS 02-Aug-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation The myosin chaperone UNC-45 is organized in tandem modules to support myofilament formation in C. elegans. The myosin chaperone UNC-45 is organized in tandem modules to support myofilament formation in C. elegans. Cell (0092-8674) 2013 imex curation Gazda L., Pokrzywa W., Hellerschmied D., Löwe T., Forné I., Mueller-Planitz F., Hoppe T., Clausen T. thorsten.hoppe@uni-koeln.de, clausen@imp.univie.ac.at 02-Aug-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column weight by comassie molecular weight estimation by coomasie staining weight by comassie Gazda L., Pokrzywa W., Hellerschmied D., Löwe T., Forné I., Mueller-Planitz F., Hoppe T., Clausen T. thorsten.hoppe@uni-koeln.de, clausen@imp.univie.ac.at Accepted 2013-JUN-27 by PPORRAS 02-Aug-2013: Contacted by IntAct-Help. Cell (0092-8674) 2013 Only protein-protein interactions imex curation g5eg62_caeel unc-45 CELE_F30H5.1 F30H5.1 protein protein caeel Caenorhabditis elegans MVARVQTAEEIRDEGNAAVKDQDYIKADELYTEALQLTTDEDKALRPVLYRNRAMARLKRDDFEGAQSDCTKALEFDGADVKALFRRSLAREQLGNVGPAFQDAKEALRLSPNDKGIVEVLQRLVKANNDKIKQTTSLANKVTDMEKLAFRGEAKDTEQKMTALNNLLVLCRESESGATGVWNQGALVPFVLNLINDASENEEVTVTAIRILDETIKNSVRCMKFLAMHDPDGPKSVRFVCRLMCKKSTKDFVDATGILVQRVFNAMAKMDRQKEMKPDPEVAEANKIWIIRVLLELQEMLQDPKVGAVQRETCIDLFLKNLMHMDGGIPRGWSWKFVEERGLLALLDVASQIPELCEYPVSAETRQHVAICLQRLEEDMVFDTKRTIFKEKVDMFFNALISRCTNDDEGHKYRIKLSCFLITMLQGPVDIGINLITNDQLTPIMLEMAASQDHLMQGIAAELIVATVSKHERAINMLKVGIPVLRALYDSEDPTVKVRALVGLCKIGAAGGDDISKATMKEEAVISLAKTCKKFLLETEKYSVDIRRYACEGLSYLSLDADVKEWIVDDSLLLKALVLLAKKAGALCVYTLATIYANLSNAFEKPKVDEEMVKLAQFAKHHVPETHPKDTEEYVEKRVRALVEEGAVPACVAVSKTESKNALELIARSLLAFAEYEDLRGRIIAEGGTVLCLRLTKEASGEGKIKAGHAIAKLGAKADPMISFPGQRAYEVVKPLCDLLHPDVEGKANYDSLLTLTNLASVSDSIRGRILKEKAIPKIEEFWFMTDHEHLRAAAAELLLNLLFFEKFYEETVAPGTDRLKLWVLYSAEVEEERLSRASAAGFAILTEDENACARIMDEIKSWPEVFKDIAMHEDAETQRRGLMGIANIMHSSNKLCSEIVSSEVFRVLVAVTKLGTINQERAGSTEQAKRGLEAAEKFGLIKATDREIYERENQMSTIQE DE9B7B8CC21B29E7 hsp90_caeel Heat shock protein 90 daf-21 Abnormal dauer formation protein 21 C47E8.5 protein protein caeel Caenorhabditis elegans MSENAETFAFQAEIAQLMSLIINTFYSNKEIYLRELISNASDALDKIRYQALTEPSELDTGKELFIKITPNKEEKTLTIMDTGIGMTKADLVNNLGTIAKSGTKAFMEALQAGADISMIGQFGVGFYSAFLVADKVVVTSKNNDDDSYQWESSAGGSFVVRPFNDPEVTRGTKIVMHIKEDQIDFLEERKIKEIVKKHSQFIGYPIKLVVEKEREKEVEDEEAVEAKDEEKKEGEVENVADDADKKKTKKIKEKYFEDEELNKTKPIWTRNPDDISNEEYAEFYKSLSNDWEDHLAVKHFSVEGQLEFRALLFVPQRAPFDLFENKKSKNSIKLYVRRVFIMENCEELMPEYLNFIKGVVDSEDLPLNISREMLQQSKILKVIRKNLVKKCMELIDEVAEDKDNFKKFYEQFGKNLKLGIHEDSTNRKKLSDFLRYSTSAGDEPTSLKEYVSRMKENQTQIYYITGESKDVVAASAFVERVKSRGFEVLYMCDPIDEYCVQQLKEYDGKKLVSVTKEGLELPETEEEKKKFEEDKVAYENLCKVIKDILEKKVEKVGVSNRLVSSPCCIVTSEYGWSANMERIMKAQALRDSSTMGYMAAKKHLEINPDHAIMKTLRDRVEVDKNDKTVKDLVVLLFETALLASGFSLEEPQSHASRIYRMIKLGLDIGDDEIEDSAVPSSCTAEAKIEGAEEDASRMEEVD 2B0E975A24074811 myo4_caeel Myosin-4 unc-54 myo-4 Myosin heavy chain B Uncoordinated protein 54 F11C3.3 protein protein caeel Caenorhabditis elegans MEHEKDPGWQYLRRTREQVLEDQSKPYDSKKNVWIPDPEEGYLAGEITATKGDQVTIVTARGNEVTLKKELVQEMNPPKFEKTEDMSNLSFLNDASVLHNLRSRYAAMLIYTYSGLFCVVINPYKRLPIYTDSCARMFMGKRKTEMPPHLFAVSDEAYRNMLQDHENQSMLITGESGAGKTENTKKVICYFAAVGASQQEGGAEVDPNKKKVTLEDQIVQTNPVLEAFGNAKTVRNNNSSRFGKFIRIHFNKHGRLASCDIEHYLLEKSRVIRQAPGERCYHIFYQIYSDFRPELKKELLLDLPIKDYWFVAQAELIIDGIDDVEEFQLTDEAFDILNFSAVEKQDCYRLMSAHMHMGNMKFKQRPREEQAEPDGTDEAEKASNMYGIGCEEFLKALTKPRVKVGTEWVSKGQNCEQVNWAVGAMAKGLYSRVFNWLVKKCNLTLDQKGIDRDYFIGVLDIAGFEIFDFNSFEQLWINFVNEKLQQFFNHHMFVLEQEEYAREGIQWVFIDFGLDLQACIELIEKPLGIISMLDEECIVPKATDLTLASKLVDQHLGKHPNFEKPKPPKGKQGEAHFAMRHYAGTVRYNCLNWLEKNKDPLNDTVVSAMKQSKGNDLLVEIWQDYTTQEEAAAKAKEGGGGGKKKGKSGSFMTVSMLYRESLNNLMTMLNKTHPHFIRCIIPNEKKQSGMIDAALVLNQLTCNGVLEGIRICRKGFPNRTLHPDFVQRYAILAAKEAKSDDDKKKCAEAIMSKLVNDGSLSEEMFRIGLTKVFFKAGVLAHLEDIRDEKLATILTGFQSQIRWHLGLKDRKRRMEQRAGLLIVQRNVRSWCTLRTWEWFKLYGKVKPMLKAGKEAEELEKINDKVKALEDSLAKEEKLRKELEESSAKLVEEKTSLFTNLESTKTQLSDAEERLAKLEAQQKDASKQLSELNDQLADNEDRTADVQRAKKKIEAEVEALKKQIQDLEMSLRKAESEKQSKDHQIRSLQDEMQQQDEAIAKLNKEKKHQEEINRKLMEDLQSEEDKGNHQNKVKAKLEQTLDDLEDSLEREKRARADLDKQKRKVEGELKIAQENIDESGRQRHDLENNLKKKESELHSVSSRLEDEQALVSKLQRQIKDGQSRISELEEELENERQSRSKADRAKSDLQRELEELGEKLDEQGGATAAQVEVNKKREAELAKLRRDLEEANMNHENQLGGLRKKHTDAVAELTDQLDQLNKAKAKVEKDKAQAVRDAEDLAAQLDQETSGKLNNEKLAKQFELQLTELQSKADEQSRQLQDFTSLKGRLHSENGDLVRQLEDAESQVNQLTRLKSQLTSQLEEARRTADEEARERQTVAAQAKNYQHEAEQLQESLEEEIEGKNEILRQLSKANADIQQWKARFEGEGLLKADELEDAKRRQAQKINELQEALDAANSKNASLEKTKSRLVGDLDDAQVDVERANGVASALEKKQKGFDKIIDEWRKKTDDLAAELDGAQRDLRNTSTDLFKAKNAQEELAEVVEGLRRENKSLSQEIKDLTDQLGEGGRSVHEMQKIIRRLEIEKEELQHALDEAEAALEAEESKVLRAQVEVSQIRSEIEKRIQEKEEEFENTRKNHARALESMQASLETEAKGKAELLRIKKKLEGDINELEIALDHANKANADAQKNLKRYQEQVRELQLQVEEEQRNGADTREQFFNAEKRATLLQSEKEELLVANEAAERARKQAEYEAADARDQANEANAQVSSLTSAKRKLEGEIQAIHADLDETLNEYKAAEERSKKAIADATRLAEELRQEQEHSQHVDRLRKGLEQQLKEIQVRLDEAEAAALKGGKKVIAKLEQRVRELESELDGEQRRFQDANKNLGRADRRVRELQFQVDEDKKNFERLQDLIDKLQQKLKTQKKQVEEAEELANLNLQKYKQLTHQLEDAEERADQAENSLSKMRSKSRASASVAPGLQSSASAAVIRSPSRARASDF D653C86050E846C6 hsp1_caeel Heat shock protein hsp-1 hsp-1 hsp70a F26D10.3 Heat shock 70 kDa protein A protein protein caeel Caenorhabditis elegans MSKHNAVGIDLGTTYSCVGVFMHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPHNTVFDAKRLIGRKFDDPAVQSDMKHWPFKVISAEGAKPKVQVEYKGENKIFTPEEISSMVLLKMKETAEAFLGTTVKDAVVTVPAYFNDSQRQATKDAGAIAGLNVLRIINEPTAAAIAYGLDKKGHGERNVLIFDLGGGTFDVSILTIEDGIFEVKSTAGDTHLGGEDFDNRMVNHFCAEFKRKHKKDLASNPRALRRLRTACERAKRTLSSSSQASIEIDSLFEGIDFYTNITRARFEELCADLFRSTMDPVEKSLRDAKMDKSQVHDIVLVGGSTRIPKVQKLLSDLFSGKELNKSINPDEAVAYGAAVQAAILSGDKSEAVQDLLLLDVAPLSLGIETAGGVMTALIKRNTTIPTKTAQTFTTYSDNQPGVLIQVYEGERAMTKDNNLLGKFELSGIPPAPRGVPQIEVTFDIDANGILNVSATDKSTGKQNKITITNDKGRLSKDDIERMVNEAEKYKADDEAQKDRIGAKNGLESYAFNLKQTIEDEKLKDKISPEDKKKIEDKCDEILKWLDSNQTAEKEEFEHQQKDLEGLANPIISKLYQSAGGAPPGAAPGGAAGGAGGPTIEEVD 5D7B46B812E7A1DA daf_21-unc_45 4 8 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Supp. Table 1 2.9A Rworking = 0.24 Rfree = 0.26 hsp_1-unc_45 4 8 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Supp. Table 1 3.6A Rworking = 0.23 Rfree = 0.28 daf_21-unc_45-1 5 9 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 8 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range G5EG62:p.Lys82Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K E Wed May 02 19:06:04 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys82glu' Tue Jun 30 17:34:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys82Glu' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 2A hsp_1-unc_45-1 5 8 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range G5EG62:p.Lys82Glu mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength certain certain sequence position certain certain certain sequence position certain K E Wed May 02 19:50:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys82glu' Tue Jun 30 19:53:02 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Lys82Glu' ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) direct interaction direct interaction Fig. 2A daf_21-unc_45-2 5 8 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) 9 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 2A daf_21-unc_45-3 7 9 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range c-terminal region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 2B, Supp. Fig. 2 hsp_1-unc_45-2 7 11 unspecified role unspecified role neutral component neutral component c-terminal region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 8 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 2B, Supp. 2A unc_45-daf_21 2 8 unspecified role unspecified role bait bait c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range 10 unspecified role unspecified role prey prey 9 unspecified role unspecified role prey prey association association Fig. 3D unc_45-daf_21-1 2 8 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range G5EG62:p.Asn758Tyr mutation mutation certain certain sequence position certain certain certain sequence position certain N Y Wed May 02 14:38:20 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'asn758tyr' Tue Jun 30 18:00:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Asn758Tyr' 10 unspecified role unspecified role prey prey physical association physical association Fig. 3D unc_45 1 8 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range G5EG62:p.Gln452Xaa mutation mutation certain certain sequence position certain certain certain sequence position certain Q X Glutamine 452 was mutated to unconventional amino acid p-benzoyl-L-phenylalanine (pBpa). Fri Jul 22 10:57:27 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'gln452unc' Wed May 02 19:17:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln452xaa' Tue Jun 30 19:27:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln452Xaa' G5EG62:p.[Val480Arg;Val484Arg] mutation mutation certain certain sequence position certain certain certain sequence position certain V R certain certain sequence position certain certain certain sequence position certain V R Wed May 02 15:21:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val480arg,val484arg' Tue Jun 30 16:44:04 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val480Arg;Val484Arg]' G5EG62:p.Leu121Trp mutation mutation certain certain sequence position certain certain certain sequence position certain L W Wed May 02 18:53:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu121trp' Tue Jun 30 15:52:51 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu121Trp' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 5A, Supp. Fig. 5A Chain formation depends strongly on UNC-45 concentration, with the longer chains occurring only at elevated protein concentration. unc_45-1 1 8 unspecified role unspecified role neutral component neutral component G5EG62:p.Gln452Xaa mutation mutation certain certain sequence position certain certain certain sequence position certain Q X Glutamine 452 was mutated to unconventional amino acid p-benzoyl-L-phenylalanine (pBpa). Fri Jul 22 11:00:12 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'gln452unc' Wed May 02 16:25:34 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln452xaa' Tue Jun 30 16:44:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln452Xaa' c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 5A, Supp Fig. 5A Chain formation depends strongly on UNC-45 concentration, with the longer chains occurring only at elevated protein concentration. unc_45-2 1 8 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range G5EG62:p.Gln452Xaa mutation mutation certain certain sequence position certain certain certain sequence position certain Q X Glutamine 452 was mutated to unconventional amino acid p-benzoyl-L-phenylalanine (pBpa). Fri Jul 22 10:59:08 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'gln452unc' Wed May 02 18:17:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln452xaa' Tue Jun 30 15:52:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln452Xaa' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 5A, Supp. Fig. 5A Chain formation depends strongly on UNC-45 concentration, with the longer chains occurring only at elevated protein concentration. unc_45-2 3 8 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range G5EG62:p.Gln452Xaa mutation mutation certain certain sequence position certain certain certain sequence position certain Q X Glutamine 452 was mutated to unconventional amino acid p-benzoyl-L-phenylalanine (pBpa). Fri Jul 22 11:04:28 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'gln452unc' Wed May 02 17:11:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln452xaa' Tue Jun 30 20:12:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln452Xaa' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 5B unc_45-2 3 8 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range G5EG62:p.Gln452Xaa mutation mutation certain certain sequence position certain certain certain sequence position certain Q X Glutamine 452 was mutated to unconventional amino acid p-benzoyl-L-phenylalanine (pBpa). Fri Jul 22 10:58:50 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'gln452unc' Wed May 02 19:10:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln452xaa' Tue Jun 30 16:09:29 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln452Xaa' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 5B unc_45-2 3 8 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range G5EG62:p.Gln452Xaa mutation mutation certain certain sequence position certain certain certain sequence position certain Q X Glutamine 452 was mutated to unconventional amino acid p-benzoyl-L-phenylalanine (pBpa). Fri Jul 22 10:50:21 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'gln452unc' Wed May 02 14:49:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln452xaa' Tue Jun 30 18:29:31 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln452Xaa' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 5B unc-1 3 8 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range G5EG62:p.Gln452Xaa mutation mutation certain certain sequence position certain certain certain sequence position certain Q X Glutamine 452 was mutated to unconventional amino acid p-benzoyl-L-phenylalanine (pBpa). Fri Jul 22 11:03:01 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'gln452unc' Wed May 02 18:19:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln452xaa' Tue Jun 30 16:44:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln452Xaa' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 5B unc_45-2 6 8 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range G5EG62:p.Gln452Xaa mutation mutation certain certain sequence position certain certain certain sequence position certain Q X Glutamine 452 was mutated to unconventional amino acid p-benzoyl-L-phenylalanine (pBpa). Fri Jul 22 11:08:30 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'gln452unc' Wed May 02 15:23:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln452xaa' Tue Jun 30 18:08:56 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln452Xaa' ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 5c UV-activated pBpa452 crosslinks to the Lys131/Ile132/Lys133 sequence unc_45-daf_21-2 2 10 unspecified role unspecified role prey prey 8 unspecified role unspecified role bait bait G5EG62:p.[Val480Arg;Val484Arg] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain V R certain certain sequence position certain certain certain sequence position certain V R Wed May 02 19:17:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val480arg,val484arg' Tue Jun 30 16:47:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Val480Arg;Val484Arg]' c-terminal flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association Supp. Fig. 6D