InnateDB InnateDB The CD46-Jagged1 interaction is critical for human TH1 immunity. The CD46-Jagged1 interaction is critical for human TH1 immunity. Nat. Immunol. (1529-2908) 2012 imex curation Le Friec G., Sheppard D., Whiteman P., Karsten C.M., Shamoun S.A., Laing A., Bugeon L., Dallman M.J., Melchionna T., Chillakuri C., Smith R.A., Drouet C., Couzi L., Fremeaux-Bacchi V., Köhl J., Waddington S.N., McDonnell J.M., Baker A., Handford P.A., Lea S.M., Kemper C. Species information for C3b was from Complement Technology product sheet. Notch-1(11-13) was determined from ref. 13, and CD46 was determined via correspondence. claudia.kemper@kcl.ac.uk 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions Curated by: InnateDB - a knowledge resource for innate immunity in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa predetermined predetermined participant predetermined Le Friec G., Sheppard D., Whiteman P., Karsten C.M., Shamoun S.A., Laing A., Bugeon L., Dallman M.J., Melchionna T., Chillakuri C., Smith R.A., Drouet C., Couzi L., Fremeaux-Bacchi V., Köhl J., Waddington S.N., McDonnell J.M., Baker A., Handford P.A., Lea S.M., Kemper C. Species information for C3b was from Complement Technology product sheet. Notch-1(11-13) was determined from ref. 13, and CD46 was determined via correspondence. claudia.kemper@kcl.ac.uk Accepted 2013-SEP-09 by PPORRAS 30-Sept-2013: Contacted by IntAct-Help. Nat. Immunol. (1529-2908) 2012 Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity The CD46-Jagged1 interaction is critical for human TH1 immunity. The CD46-Jagged1 interaction is critical for human TH1 immunity. Nat. Immunol. (1529-2908) 2012 imex curation Le Friec G., Sheppard D., Whiteman P., Karsten C.M., Shamoun S.A., Laing A., Bugeon L., Dallman M.J., Melchionna T., Chillakuri C., Smith R.A., Drouet C., Couzi L., Fremeaux-Bacchi V., Köhl J., Waddington S.N., McDonnell J.M., Baker A., Handford P.A., Lea S.M., Kemper C. Species information for C3b was from Complement Technology product sheet. Notch-1(11-13) was determined from ref. 13, and CD46 was determined via correspondence. claudia.kemper@kcl.ac.uk 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions Curated by: InnateDB - a knowledge resource for innate immunity in vitro In vitro nmr nuclear magnetic resonance NMR NMR nmr predetermined predetermined participant predetermined Le Friec G., Sheppard D., Whiteman P., Karsten C.M., Shamoun S.A., Laing A., Bugeon L., Dallman M.J., Melchionna T., Chillakuri C., Smith R.A., Drouet C., Couzi L., Fremeaux-Bacchi V., Köhl J., Waddington S.N., McDonnell J.M., Baker A., Handford P.A., Lea S.M., Kemper C. Species information for C3b was from Complement Technology product sheet. Notch-1(11-13) was determined from ref. 13, and CD46 was determined via correspondence. claudia.kemper@kcl.ac.uk Accepted 2013-SEP-09 by PPORRAS 30-Sept-2013: Contacted by IntAct-Help. Nat. Immunol. (1529-2908) 2012 Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity The CD46-Jagged1 interaction is critical for human TH1 immunity. The CD46-Jagged1 interaction is critical for human TH1 immunity. Nat. Immunol. (1529-2908) 2012 imex curation Le Friec G., Sheppard D., Whiteman P., Karsten C.M., Shamoun S.A., Laing A., Bugeon L., Dallman M.J., Melchionna T., Chillakuri C., Smith R.A., Drouet C., Couzi L., Fremeaux-Bacchi V., Köhl J., Waddington S.N., McDonnell J.M., Baker A., Handford P.A., Lea S.M., Kemper C. Species information for C3b was from Complement Technology product sheet. Notch-1(11-13) was determined from ref. 13, and CD46 was determined via correspondence. claudia.kemper@kcl.ac.uk 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions Curated by: InnateDB - a knowledge resource for innate immunity human-t-cell Homo sapiens t-cell Peripheral blood T-lymphocytes confocal microscopy confocal microscopy immunostaining immunostaining Le Friec G., Sheppard D., Whiteman P., Karsten C.M., Shamoun S.A., Laing A., Bugeon L., Dallman M.J., Melchionna T., Chillakuri C., Smith R.A., Drouet C., Couzi L., Fremeaux-Bacchi V., Köhl J., Waddington S.N., McDonnell J.M., Baker A., Handford P.A., Lea S.M., Kemper C. Species information for C3b was from Complement Technology product sheet. Notch-1(11-13) was determined from ref. 13, and CD46 was determined via correspondence. claudia.kemper@kcl.ac.uk Accepted 2013-SEP-09 by PPORRAS 30-Sept-2013: Contacted by IntAct-Help. Nat. Immunol. (1529-2908) 2012 Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity The CD46-Jagged1 interaction is critical for human TH1 immunity. The CD46-Jagged1 interaction is critical for human TH1 immunity. Nat. Immunol. (1529-2908) 2012 imex curation Le Friec G., Sheppard D., Whiteman P., Karsten C.M., Shamoun S.A., Laing A., Bugeon L., Dallman M.J., Melchionna T., Chillakuri C., Smith R.A., Drouet C., Couzi L., Fremeaux-Bacchi V., Köhl J., Waddington S.N., McDonnell J.M., Baker A., Handford P.A., Lea S.M., Kemper C. Species information for C3b was from Complement Technology product sheet. Notch-1(11-13) was determined from ref. 13, and CD46 was determined via correspondence. claudia.kemper@kcl.ac.uk 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions Curated by: InnateDB - a knowledge resource for innate immunity in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Le Friec G., Sheppard D., Whiteman P., Karsten C.M., Shamoun S.A., Laing A., Bugeon L., Dallman M.J., Melchionna T., Chillakuri C., Smith R.A., Drouet C., Couzi L., Fremeaux-Bacchi V., Köhl J., Waddington S.N., McDonnell J.M., Baker A., Handford P.A., Lea S.M., Kemper C. Species information for C3b was from Complement Technology product sheet. Notch-1(11-13) was determined from ref. 13, and CD46 was determined via correspondence. claudia.kemper@kcl.ac.uk Accepted 2013-SEP-09 by PPORRAS 30-Sept-2013: Contacted by IntAct-Help. Nat. Immunol. (1529-2908) 2012 Only protein-protein interactions imex curation Curated by: InnateDB - a knowledge resource for innate immunity notc1_human Neurogenic locus notch homolog protein 1 Translocation-associated notch protein TAN-1 NOTCH1 TAN1 protein protein human Homo sapiens Human MPPLLAPLLCLALLPALAARGPRCSQPGETCLNGGKCEAANGTEACVCGGAFVGPRCQDPNPCLSTPCKNAGTCHVVDRRGVADYACSCALGFSGPLCLTPLDNACLTNPCRNGGTCDLLTLTEYKCRCPPGWSGKSCQQADPCASNPCANGGQCLPFEASYICHCPPSFHGPTCRQDVNECGQKPGLCRHGGTCHNEVGSYRCVCRATHTGPNCERPYVPCSPSPCQNGGTCRPTGDVTHECACLPGFTGQNCEENIDDCPGNNCKNGGACVDGVNTYNCRCPPEWTGQYCTEDVDECQLMPNACQNGGTCHNTHGGYNCVCVNGWTGEDCSENIDDCASAACFHGATCHDRVASFYCECPHGRTGLLCHLNDACISNPCNEGSNCDTNPVNGKAICTCPSGYTGPACSQDVDECSLGANPCEHAGKCINTLGSFECQCLQGYTGPRCEIDVNECVSNPCQNDATCLDQIGEFQCICMPGYEGVHCEVNTDECASSPCLHNGRCLDKINEFQCECPTGFTGHLCQYDVDECASTPCKNGAKCLDGPNTYTCVCTEGYTGTHCEVDIDECDPDPCHYGSCKDGVATFTCLCRPGYTGHHCETNINECSSQPCRHGGTCQDRDNAYLCFCLKGTTGPNCEINLDDCASSPCDSGTCLDKIDGYECACEPGYTGSMCNINIDECAGNPCHNGGTCEDGINGFTCRCPEGYHDPTCLSEVNECNSNPCVHGACRDSLNGYKCDCDPGWSGTNCDINNNECESNPCVNGGTCKDMTSGYVCTCREGFSGPNCQTNINECASNPCLNQGTCIDDVAGYKCNCLLPYTGATCEVVLAPCAPSPCRNGGECRQSEDYESFSCVCPTGWQGQTCEVDINECVLSPCRHGASCQNTHGGYRCHCQAGYSGRNCETDIDDCRPNPCHNGGSCTDGINTAFCDCLPGFRGTFCEEDINECASDPCRNGANCTDCVDSYTCTCPAGFSGIHCENNTPDCTESSCFNGGTCVDGINSFTCLCPPGFTGSYCQHDVNECDSQPCLHGGTCQDGCGSYRCTCPQGYTGPNCQNLVHWCDSSPCKNGGKCWQTHTQYRCECPSGWTGLYCDVPSVSCEVAAQRQGVDVARLCQHGGLCVDAGNTHHCRCQAGYTGSYCEDLVDECSPSPCQNGATCTDYLGGYSCKCVAGYHGVNCSEEIDECLSHPCQNGGTCLDLPNTYKCSCPRGTQGVHCEINVDDCNPPVDPVSRSPKCFNNGTCVDQVGGYSCTCPPGFVGERCEGDVNECLSNPCDARGTQNCVQRVNDFHCECRAGHTGRRCESVINGCKGKPCKNGGTCAVASNTARGFICKCPAGFEGATCENDARTCGSLRCLNGGTCISGPRSPTCLCLGPFTGPECQFPASSPCLGGNPCYNQGTCEPTSESPFYRCLCPAKFNGLLCHILDYSFGGGAGRDIPPPLIEEACELPECQEDAGNKVCSLQCNNHACGWDGGDCSLNFNDPWKNCTQSLQCWKYFSDGHCDSQCNSAGCLFDGFDCQRAEGQCNPLYDQYCKDHFSDGHCDQGCNSAECEWDGLDCAEHVPERLAAGTLVVVVLMPPEQLRNSSFHFLRELSRVLHTNVVFKRDAHGQQMIFPYYGREEELRKHPIKRAAEGWAAPDALLGQVKASLLPGGSEGGRRRRELDPMDVRGSIVYLEIDNRQCVQASSQCFQSATDVAAFLGALASLGSLNIPYKIEAVQSETVEPPPPAQLHFMYVAAAAFVLLFFVGCGVLLSRKRRRQHGQLWFPEGFKVSEASKKKRREPLGEDSVGLKPLKNASDGALMDDNQNEWGDEDLETKKFRFEEPVVLPDLDDQTDHRQWTQQHLDAADLRMSAMAPTPPQGEVDADCMDVNVRGPDGFTPLMIASCSGGGLETGNSEEEEDAPAVISDFIYQGASLHNQTDRTGETALHLAARYSRSDAAKRLLEASADANIQDNMGRTPLHAAVSADAQGVFQILIRNRATDLDARMHDGTTPLILAARLAVEGMLEDLINSHADVNAVDDLGKSALHWAAAVNNVDAAVVLLKNGANKDMQNNREETPLFLAAREGSYETAKVLLDHFANRDITDHMDRLPRDIAQERMHHDIVRLLDEYNLVRSPQLHGAPLGGTPTLSPPLCSPNGYLGSLKPGVQGKKVRKPSSKGLACGSKEAKDLKARRKKSQDGKGCLLDSSGMLSPVDSLESPHGYLSDVASPPLLPSPFQQSPSVPLNHLPGMPDTHLGIGHLNVAAKPEMAALGGGGRLAFETGPPRLSHLPVASGTSTVLGSSSGGALNFTVGGSTSLNGQCEWLSRLQSGMVPNQYNPLRGSVAPGPLSTQAPSLQHGMVGPLHSSLAASALSQMMSYQGLPSTRLATQPHLVQTQQVQPQNLQMQQQNLQPANIQQQQSLQPPPPPPQPHLGVSSAASGHLGRSFLSGEPSQADVQPLGPSSLAVHTILPQESPALPTSLPSSLVPPVTAAQFLTPPSQHSYSSPVDNTPSHQLQVPEHPFLTPSPESPDQWSSSSPHSNVSDWSEGVSSPPTSMQSQIARIPEAFK DIP protein P46531 original sequence version: 173 DIP protein P46531 original sequence version: 103 DIP protein P46531 original sequence version: 109 DIP protein P46531 original sequence version: 127 DIP protein P46531 original sequence version: 137 DIP protein P46531 original sequence version: 186 DIP protein P46531 original sequence version: 150 DIP protein P46531 original sequence version: 142 DIP protein P46531 original sequence version: 181 E173C872D195F028 jag1_human Protein jagged-1 JAG1 JAGL1 protein protein human Homo sapiens Human MRSPRTRGRSGRPLSLLLALLCALRAKVCGASGQFELEILSMQNVNGELQNGNCCGGARNPGDRKCTRDECDTYFKVCLKEYQSRVTAGGPCSFGSGSTPVIGGNTFNLKASRGNDRNRIVLPFSFAWPRSYTLLVEAWDSSNDTVQPDSIIEKASHSGMINPSRQWQTLKQNTGVAHFEYQIRVTCDDYYYGFGCNKFCRPRDDFFGHYACDQNGNKTCMEGWMGPECNRAICRQGCSPKHGSCKLPGDCRCQYGWQGLYCDKCIPHPGCVHGICNEPWQCLCETNWGGQLCDKDLNYCGTHQPCLNGGTCSNTGPDKYQCSCPEGYSGPNCEIAEHACLSDPCHNRGSCKETSLGFECECSPGWTGPTCSTNIDDCSPNNCSHGGTCQDLVNGFKCVCPPQWTGKTCQLDANECEAKPCVNAKSCKNLIASYYCDCLPGWMGQNCDININDCLGQCQNDASCRDLVNGYRCICPPGYAGDHCERDIDECASNPCLNGGHCQNEINRFQCLCPTGFSGNLCQLDIDYCEPNPCQNGAQCYNRASDYFCKCPEDYEGKNCSHLKDHCRTTPCEVIDSCTVAMASNDTPEGVRYISSNVCGPHGKCKSQSGGKFTCDCNKGFTGTYCHENINDCESNPCRNGGTCIDGVNSYKCICSDGWEGAYCETNINDCSQNPCHNGGTCRDLVNDFYCDCKNGWKGKTCHSRDSQCDEATCNNGGTCYDEGDAFKCMCPGGWEGTTCNIARNSSCLPNPCHNGGTCVVNGESFTCVCKEGWEGPICAQNTNDCSPHPCYNSGTCVDGDNWYRCECAPGFAGPDCRININECQSSPCAFGATCVDEINGYRCVCPPGHSGAKCQEVSGRPCITMGSVIPDGAKWDDDCNTCQCLNGRIACSKVWCGPRPCLLHKGHSECPSGQSCIPILDDQCFVHPCTGVGECRSSSLQPVKTKCTSDSYYQDNCANITFTFNKEMMSPGLTTEHICSELRNLNILKNVSAEYSIYIACEPSPSANNEIHVAISAEDIRDDGNPIKEITDKIIDLVSKRDGNSSLIAAVAEVRVQRRPLKNRTDFLVPLLSSVLTVAWICCLVTAFYWCLRKRRKPGSHTHSASEDNTTNNVREQLNQIKNPIEKHGANTVPIKDYENKNSKMSKIRTHNSEVEEDDMDKHQQKARFAKQPAYTLVDREEKPPNGTPTKHPNWTNKQDNRDLESAQSLNRMEYIV DIP protein P78504 original sequence version: 95 F36EE9FBF64DF162 mcp_human Membrane cofactor protein MCP MIC10 TLX Trophoblast leukocyte common antigen CD46 protein protein human Homo sapiens Human MEPPGRRECPFPSWRFPGLLLAAMVLLLYSFSDACEEPPTFEAMELIGKPKPYYEIGERVDYKCKKGYFYIPPLATHTICDRNHTWLPVSDDACYRETCPYIRDPLNGQAVPANGTYEFGYQMHFICNEGYYLIGEEILYCELKGSVAIWSGKPPICEKVLCTPPPKIKNGKHTFSEVEVFEYLDAVTYSCDPAPGPDPFSLIGESTIYCGDNSVWSRAAPECKVVKCRFPVVENGKQISGFGKKFYYKATVMFECDKGFYLDGSDTIVCDSNSTWDPPVPKCLKVLPPSSTKPPALSHSVSTSSTTKSPASSASGPRPTYKPPVSNYPGYPKPEEGILDSLDVWVIAVIVIAIVVGVAVICVVPYRYLQRRKKKGTYLTDETHREVKFTSL DIP protein P15529 original sequence version: 143 85FE0CF100EA703E co3_human Complement C3 C3 CPAMD1 C3 and PZP-like alpha-2-macroglobulin domain-containing protein 1 protein protein human Homo sapiens Human MGPTSGPSLLLLLLTHLPLALGSPMYSIITPNILRLESEETMVLEAHDAQGDVPVTVTVHDFPGKKLVLSSEKTVLTPATNHMGNVTFTIPANREFKSEKGRNKFVTVQATFGTQVVEKVVLVSLQSGYLFIQTDKTIYTPGSTVLYRIFTVNHKLLPVGRTVMVNIENPEGIPVKQDSLSSQNQLGVLPLSWDIPELVNMGQWKIRAYYENSPQQVFSTEFEVKEYVLPSFEVIVEPTEKFYYIYNEKGLEVTITARFLYGKKVEGTAFVIFGIQDGEQRISLPESLKRIPIEDGSGEVVLSRKVLLDGVQNPRAEDLVGKSLYVSATVILHSGSDMVQAERSGIPIVTSPYQIHFTKTPKYFKPGMPFDLMVFVTNPDGSPAYRVPVAVQGEDTVQSLTQGDGVAKLSINTHPSQKPLSITVRTKKQELSEAEQATRTMQALPYSTVGNSNNYLHLSVLRTELRPGETLNVNFLLRMDRAHEAKIRYYTYLIMNKGRLLKAGRQVREPGQDLVVLPLSITTDFIPSFRLVAYYTLIGASGQREVVADSVWVDVKDSCVGSLVVKSGQSEDRQPVPGQQMTLKIEGDHGARVVLVAVDKGVFVLNKKNKLTQSKIWDVVEKADIGCTPGSGKDYAGVFSDAGLTFTSSSGQQTAQRAELQCPQPAARRRRSVQLTEKRMDKVGKYPKELRKCCEDGMRENPMRFSCQRRTRFISLGEACKKVFLDCCNYITELRRQHARASHLGLARSNLDEDIIAEENIVSRSEFPESWLWNVEDLKEPPKNGISTKLMNIFLKDSITTWEILAVSMSDKKGICVADPFEVTVMQDFFIDLRLPYSVVRNEQVEIRAVLYNYRQNQELKVRVELLHNPAFCSLATTKRRHQQTVTIPPKSSLSVPYVIVPLKTGLQEVEVKAAVYHHFISDGVRKSLKVVPEGIRMNKTVAVRTLDPERLGREGVQKEDIPPADLSDQVPDTESETRILLQGTPVAQMTEDAVDAERLKHLIVTPSGCGEQNMIGMTPTVIAVHYLDETEQWEKFGLEKRQGALELIKKGYTQQLAFRQPSSAFAAFVKRAPSTWLTAYVVKVFSLAVNLIAIDSQVLCGAVKWLILEKQKPDGVFQEDAPVIHQEMIGGLRNNNEKDMALTAFVLISLQEAKDICEEQVNSLPGSITKAGDFLEANYMNLQRSYTVAIAGYALAQMGRLKGPLLNKFLTTAKDKNRWEDPGKQLYNVEATSYALLALLQLKDFDFVPPVVRWLNEQRYYGGGYGSTQATFMVFQALAQYQKDAPDHQELNLDVSLQLPSRSSKITHRIHWESASLLRSEETKENEGFTVTAEGKGQGTLSVVTMYHAKAKDQLTCNKFDLKVTIKPAPETEKRPQDAKNTMILEICTRYRGDQDATMSILDISMMTGFAPDTDDLKQLANGVDRYISKYELDKAFSDRNTLIIYLDKVSHSEDDCLAFKVHQYFNVELIQPGAVKVYAYYNLEESCTRFYHPEKEDGKLNKLCRDELCRCAEENCFIQKSDDKVTLEERLDKACEPGVDYVYKTRLVKVQLSNDFDEYIMAIEQTIKSGSDEVQVGQQRTFISPIKCREALKLEEKKHYLMWGLSSDFWGEKPNLSYIIGKDTWVEHWPEEDECQDEENQKQCQDLGAFTESMVVFGCPN DIP protein P01024 original sequence version: 140 DIP protein P01024 original sequence version: 148 30C2832A9E75FFC4 cd46-c3 1 7 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind range range range range direct interaction direct interaction Fig. 1A. cd46-jag1 1 6 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind range range range range 7 unspecified role unspecified role bait bait direct interaction direct interaction Fig. 1A. jag1-notch1 1 6 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind range range range range direct interaction direct interaction Fig. 1B jag1-cd46 1 6 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey direct interaction direct interaction Fig. 1B cd46-jag1-1 4 7 unspecified role unspecified role bait bait region sufficient to bind sufficient binding region sufficient to bind range range range range 6 unspecified role unspecified role prey prey direct interaction direct interaction Fig. 2A, 2B, 2C, 2D. Kd for CD46(1-4) = 8 uM, CD46(1-3) = 8.8 uM. cd46-jag1-2 2 6 unspecified role unspecified role neutral component neutral component 7 unspecified role unspecified role neutral component neutral component region sufficient to bind sufficient binding region sufficient to bind range range range range direct interaction direct interaction Fig. 2E, 2F. cd46-jag1-3 3 7 unspecified role unspecified role neutral component neutral component 6 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 4C. jag1-notch1-1 3 6 unspecified role unspecified role neutral component neutral component 5 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig. 4C anti-CD3 and anti-CD46