MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it SAS-6 coiled-coil structure and interaction with SAS-5 suggest a regulatory mechanism in C. elegans centriole assembly. SAS-6 coiled-coil structure and interaction with SAS-5 suggest a regulatory mechanism in C. elegans centriole assembly. EMBO J. (0261-4189) 2012 imex curation Qiao R., Cabral G., Lettman MM., Dammermann A., Dong G. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Qiao R., Cabral G., Lettman MM., Dammermann A., Dong G. EMBO J. (0261-4189) 2012 Only protein-protein interactions imex curation SAS-6 coiled-coil structure and interaction with SAS-5 suggest a regulatory mechanism in C. elegans centriole assembly. SAS-6 coiled-coil structure and interaction with SAS-5 suggest a regulatory mechanism in C. elegans centriole assembly. EMBO J. (0261-4189) 2012 imex curation Qiao R., Cabral G., Lettman MM., Dammermann A., Dong G. Only protein-protein interactions in vitro In vitro sls static light scattering sls predetermined predetermined participant predetermined Qiao R., Cabral G., Lettman MM., Dammermann A., Dong G. EMBO J. (0261-4189) 2012 Only protein-protein interactions imex curation SAS-6 coiled-coil structure and interaction with SAS-5 suggest a regulatory mechanism in C. elegans centriole assembly. SAS-6 coiled-coil structure and interaction with SAS-5 suggest a regulatory mechanism in C. elegans centriole assembly. EMBO J. (0261-4189) 2012 imex curation Qiao R., Cabral G., Lettman MM., Dammermann A., Dong G. Only protein-protein interactions in vitro In vitro molecular sieving molecular sieving Gel Filtration Size Exclusion Chromatography Size Exclusion Chromatography Gel Filtration Sizing column predetermined predetermined participant predetermined Qiao R., Cabral G., Lettman MM., Dammermann A., Dong G. EMBO J. (0261-4189) 2012 Only protein-protein interactions imex curation SAS-6 coiled-coil structure and interaction with SAS-5 suggest a regulatory mechanism in C. elegans centriole assembly. SAS-6 coiled-coil structure and interaction with SAS-5 suggest a regulatory mechanism in C. elegans centriole assembly. EMBO J. (0261-4189) 2012 imex curation Qiao R., Cabral G., Lettman MM., Dammermann A., Dong G. Only protein-protein interactions in vitro In vitro itc isothermal titration calorimetry ITC ITC itc predetermined predetermined participant predetermined Qiao R., Cabral G., Lettman MM., Dammermann A., Dong G. EMBO J. (0261-4189) 2012 Only protein-protein interactions imex curation SAS-6 coiled-coil structure and interaction with SAS-5 suggest a regulatory mechanism in C. elegans centriole assembly. SAS-6 coiled-coil structure and interaction with SAS-5 suggest a regulatory mechanism in C. elegans centriole assembly. EMBO J. (0261-4189) 2012 imex curation Qiao R., Cabral G., Lettman MM., Dammermann A., Dong G. Only protein-protein interactions in vitro In vitro dls dynamic light scattering dls predetermined predetermined participant predetermined Qiao R., Cabral G., Lettman MM., Dammermann A., Dong G. EMBO J. (0261-4189) 2012 Only protein-protein interactions imex curation SAS-6 coiled-coil structure and interaction with SAS-5 suggest a regulatory mechanism in C. elegans centriole assembly. SAS-6 coiled-coil structure and interaction with SAS-5 suggest a regulatory mechanism in C. elegans centriole assembly. EMBO J. (0261-4189) 2012 imex curation Qiao R., Cabral G., Lettman MM., Dammermann A., Dong G. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction experimental particp experimental participant identification experimental particp Qiao R., Cabral G., Lettman MM., Dammermann A., Dong G. EMBO J. (0261-4189) 2012 Only protein-protein interactions imex curation sas5_caeel Spindle assembly abnormal protein 5 sas-5 F35B12.5 protein protein caeel Caenorhabditis elegans MNNYDDLPCSIYFKKPTVQEFVDQPRVFEDSEVPAFQEVLQLPQERTKPPVPSTQPIVAAVEVAKKKSCLSAPKPRKEPPSHPALRQKTVAFGKTVNVSQTVEGTSRNSKKVLASTMSAQNTTTTEEQAAENWRDAMKTELQTIRTEIQEETARRQEELNAQNLVKMQELMSNFFQKITIPKQQAIEPVEKDKENFHESPRQSRQQKPASKIASAREVIKRDGVIPPEALTIIEQRLRSDPMFRQQIDNVLADAECDANRAAYSPPPPMSEVRYGSGVNPALMRETLTVERSIRYDNGLASIDSRQWTNERRDNRAPDSYRTYEPDQPCHSLYQKGQSISYYPSEAAGKTTARNNRTGYYVEDSSDHEEDVVVNKRGQNYHEQAVPETPAERERRIREKYARRK D86EBE73413E5DAE sas6_caeel Spindle assembly abnormal protein 6 sas-6 Y45F10D.9 protein protein caeel Caenorhabditis elegans MTSKIALFDQTLIASLLQPLSLNQPDFKAYKTKVKLKISEQRNETSGEKELKFEISRSDDFEFLFSETLNNEKYQILARDHDLTVDFDAFPKVIIQHLLCKNIVKNLEEDGEVDARKKAGYHSIADPGKPTEINIILDAEKNFCSFELFSKTPISKGKIFSIKLHAVRGDHLISHLLKICSSQAVKLSTFYKSADELASLRQKCGDLEKQVEKLSGVKEEFEEMSEKFKELEDEVELVKEERENIRLLVEDKEDEVADLKQDTESLQKQLEENQEELEIVGNMLREEQGKVDQLQKRNVAHQKEIGKLRAELGTAQRNLEKADQLLKRNSQQQNQQSLDMRKLGELEADLKEKDSMVESLTETIGILRKELENEKLKAAENMDSFEKLSMENENLKEKIAHYRAQRFSPAPSGLPGLQTGLTNRLTPSFKPVLGPHTPYGANLNSRTPFRDNTTLNFQNSTIATPHAFRFNSQLIADETTGSSVTNTPPAQR 118ED9B5EF6357A6 sas-2 6 SAS-6 8 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Stoichiometry: 2.0 direct interaction direct interaction mint t1 f2 sf2 Coordinates and structure factors have been deposited in the Protein Data Bank (PDB) under accession code 4GKW. sas_6-sas_5-2 1 SAS-5 7 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined SAS-6 8 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction f3b mint sas_5-sas_6 1 SAS-5 7 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined SAS-6 8 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction mint f1g sf1 sas_6-sas_5-3 1 SAS-6 8 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined SAS-5 7 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction f1c mint sas_6-sas_5-4 1 SAS-6 8 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait purified purified O62479:p.Glu278Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A Tue Jul 12 10:49:50 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 15:10:24 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu278ala' Tue Jun 30 19:00:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu278Ala' O62479:p.Glu275Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A Tue Jul 12 10:06:57 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 20:07:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu275ala' Tue Jun 30 18:45:59 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu275Ala' his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O62479:p.Glu276Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A Tue Jul 12 10:07:02 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 19:45:35 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu276ala' Tue Jun 30 18:56:16 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu276Ala' SAS-5 7 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction f3d mint sas_6-sas_5-5 1 SAS-5 7 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined SAS-6 8 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O62479:p.Glu287Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A Tue Jul 12 10:05:13 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 17:01:37 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu287ala' Tue Jun 30 18:48:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu287Ala' direct interaction direct interaction f3d mint sas_6-sas_5-6 1 SAS-6 8 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait purified purified O62479:p.Ile279Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I A Tue Jul 12 10:05:22 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 14:33:49 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile279ala' Tue Jun 30 17:30:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile279Ala' O62479:p.Met283Ala mutation decreasing interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain M A Tue Jul 12 10:48:51 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation decreasing' Wed May 02 19:28:41 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met283ala' Tue Jun 30 17:02:52 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met283Ala' his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined SAS-5 7 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction f3d mint sas_6-sas_5-7 1 SAS-6 8 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined SAS-5 7 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey purified purified Q20010:p.Arg397Cys mutation disrupting interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R C Tue Jul 12 10:24:12 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 19:23:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg397cys' Tue Jun 30 16:59:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg397Cys' mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction f3f mint sas_6-sas_5-8 1 SAS-5 7 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Q20010:p.Ile396Ala mutation disrupting interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I A Tue Jul 12 10:24:24 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 17:50:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile396ala' Tue Jun 30 17:54:50 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile396Ala' SAS-6 8 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction f3f mint sas_6-sas_5-9 1 SAS-5 7 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey purified purified Q20010:p.Tyr400Ala mutation disrupting interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y A Tue Jul 12 10:59:19 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 18:57:39 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr400ala' Tue Jun 30 19:38:07 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr400Ala' mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined SAS-6 8 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction f3f mint sas_6-sas_5-10 1 SAS-6 8 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait purified purified his tag his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain SAS-5 7 weight by comassie molecular weight estimation by coomasie staining weight by comassie experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey purified purified Q20010:p.Arg403Ala mutation disrupting interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Tue Jul 12 10:22:35 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 17:00:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg403ala' Tue Jun 30 18:46:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg403Ala' mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined direct interaction direct interaction f3f mint sas_5-sas_6-1 4 SAS-6 8 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain SAS-5 7 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q20010:p.Arg403Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R A Tue Jul 12 10:53:18 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 18:17:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg403ala' Tue Jun 30 19:08:41 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg403Ala' 85 83 direct interaction direct interaction mint sf4b sas_5-sas_6-2 4 SAS-5 7 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q20010:p.Tyr400Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain Y A Tue Jul 12 10:11:26 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 17:58:12 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr400ala' Tue Jun 30 19:44:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr400Ala' SAS-6 8 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 89 92 direct interaction direct interaction mint sf4b sas_5-sas_6-3 4 SAS-6 8 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain SAS-5 7 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q20010:p.Ile396Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I A Tue Jul 12 10:11:41 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 15:32:46 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile396ala' Tue Jun 30 18:39:13 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile396Ala' 95 97 direct interaction direct interaction mint sf4b sas_5-sas_6-4 4 SAS-6 8 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain SAS-5 7 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Q20010:p.Arg397Cys mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain R C Tue Jul 12 10:52:12 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 15:13:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg397cys' Tue Jun 30 18:46:01 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg397Cys' 103 101 direct interaction direct interaction mint sf4b sas_5-sas_6-5 4 SAS-5 7 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain SAS-6 8 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified O62479:p.Glu276Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A Tue Jul 12 10:11:17 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 15:27:32 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu276ala' Tue Jun 30 16:47:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu276Ala' binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain O62479:p.Glu275Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A Tue Jul 12 10:08:08 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 19:36:52 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu275ala' Tue Jun 30 19:47:26 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu275Ala' O62479:p.Glu278Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A Tue Jul 12 10:08:06 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 14:46:10 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu278ala' Tue Jun 30 19:06:17 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu278Ala' 107 110 direct interaction direct interaction mint sf4a sas_5-sas_6-6 4 SAS-6 8 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain O62479:p.Ile279Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain I A Tue Jul 12 10:08:24 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 16:18:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ile279ala' Tue Jun 30 19:29:36 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ile279Ala' O62479:p.Met283Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain M A Tue Jul 12 10:50:39 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 19:26:59 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'met283ala' Tue Jun 30 18:41:37 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Met283Ala' SAS-5 7 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 115 119 direct interaction direct interaction mint sf4a sas_5-sas_6-7 4 SAS-5 7 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain SAS-6 8 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified O62479:p.Glu287Ala mutation disrupting interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A Tue Jul 12 10:50:20 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 16:55:58 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu287ala' Tue Jun 30 20:22:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu287Ala' binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 122 125 direct interaction direct interaction mint sf4a sas_5-sas_6-8 4 SAS-5 7 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain SAS-6 8 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain O62479:p.Glu286Ala mutation disrupting interaction mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain E A Tue Jul 12 10:50:28 BST 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'mutation disrupting' Wed May 02 20:26:23 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu286ala' Tue Jun 30 17:49:49 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu286Ala' 128 130 direct interaction direct interaction mint sf4a sas_6-sas_6 4 SAS-6 8 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain SAS-6 8 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain 136 134 direct interaction direct interaction f5b mint To test this hypothesis, we carried out a dilution ITC experiment that has been used successfully for analysing the dissociation equilibrium of other proteins (Lovatt et al, 1996). In this assay, a series of small aliquots of concentrated SAS-6 CCD were injected into a large volume of buffer, which generated a sequence of endothermic heat pulses, characteristic of molecular dissociation (Figure 5B, upper panel). It has been reported that the Kd of the SAS-6 coiledcoil dimer is B0.9 mM (Kitagawa et al, 2011b). The fit dissociation curve had a Kdiss of 56.2±7.6 mM and DHdiss of 2.84±0.05 kcal/mol, which suggests a dimer-tetramer equilibrium (Figure 5B, lower panel). The tetrameric association of the SAS-6 CCD is apparently much weaker than the coiled-coil dimer and thus may not always survive the grid preparation for rotary metal shadowing as the coiled-coil dimer does. sas_5-sas_6-9 5 SAS-5 7 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain SAS-6 8 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction mint sf6c Moreover, examination using dynamic light scattering also indicates that the SAS-5 CTD shifts the equilibrium between two species of the SAS-6 CCD into one species when mixing the two proteins in a stoichiometric 1:1 ratio (Supplementary Figure S6). sas-2 5 SAS-6 8 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Stoichiometry: 2.0 direct interaction direct interaction mint sf6b Moreover, examination using dynamic light scattering also indicates that the SAS-5 CTD shifts the equilibrium between two species of the SAS-6 CCD into one species when mixing the two proteins in a stoichiometric 1:1 ratio (Supplementary Figure S6). sas_5-sas_6-10 2 SAS-5 7 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Stoichiometry: 2.1 SAS-6 8 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain Stoichiometry: 1.2 direct interaction direct interaction f5e mint As shown in Figure 5E, analysis of the complex by static light scattering (SLS) indicated that the complex is a hetero-trimer (Mw B80 kDa; Mws of the SAS-6 CCD monomer and the MBP SAS-5 CTD are 19 and 44 kDa, respectively). sas_5-sas_5-2 3 SAS-5 7 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain SAS-5 7 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component purified purified mbp tag mbp tag maltose binding protein tag maltose binding protein tag maltose binding protein tag mbp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction mint sf7b Using size exclusion chromatography and negative staining electron microscopy, we found that SAS-5 forms large aggregates (Supplementary Figure S7).