MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it Cacnb4 directly couples electrical activity to gene expression, a process defective in juvenile epilepsy. Cacnb4 directly couples electrical activity to gene expression, a process defective in juvenile epilepsy. EMBO J. (0261-4189) 2012 imex curation Tadmouri A., Kiyonaka S., Barbado M., Rousset M., Fablet K., Sawamura S., Bahembera E., Pernet-Gallay K., Arnoult C., Miki T., Sadoul K., Gory-Faure S., Lambrecht C., Lesage F., Akiyama S., Khochbin S., Baulande S., Janssens V., Andrieux A., Dolmetsch R., Ronjat M., Mori Y., De Waard M. Only protein-protein interactions crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Tadmouri A., Kiyonaka S., Barbado M., Rousset M., Fablet K., Sawamura S., Bahembera E., Pernet-Gallay K., Arnoult C., Miki T., Sadoul K., Gory-Faure S., Lambrecht C., Lesage F., Akiyama S., Khochbin S., Baulande S., Janssens V., Andrieux A., Dolmetsch R., Ronjat M., Mori Y., De Waard M. EMBO J. (0261-4189) 2012 Only protein-protein interactions imex curation Cacnb4 directly couples electrical activity to gene expression, a process defective in juvenile epilepsy. Cacnb4 directly couples electrical activity to gene expression, a process defective in juvenile epilepsy. EMBO J. (0261-4189) 2012 imex curation Tadmouri A., Kiyonaka S., Barbado M., Rousset M., Fablet K., Sawamura S., Bahembera E., Pernet-Gallay K., Arnoult C., Miki T., Sadoul K., Gory-Faure S., Lambrecht C., Lesage F., Akiyama S., Khochbin S., Baulande S., Janssens V., Andrieux A., Dolmetsch R., Ronjat M., Mori Y., De Waard M. Only protein-protein interactions crigr-cho chinese hamster ovary cell line cho chinese hamster ovary cell line Also called LR73 cells. fluorescence imaging fluorescence microscopy fluorescence imaging experimental particp experimental participant identification experimental particp Tadmouri A., Kiyonaka S., Barbado M., Rousset M., Fablet K., Sawamura S., Bahembera E., Pernet-Gallay K., Arnoult C., Miki T., Sadoul K., Gory-Faure S., Lambrecht C., Lesage F., Akiyama S., Khochbin S., Baulande S., Janssens V., Andrieux A., Dolmetsch R., Ronjat M., Mori Y., De Waard M. EMBO J. (0261-4189) 2012 Only protein-protein interactions imex curation Cacnb4 directly couples electrical activity to gene expression, a process defective in juvenile epilepsy. Cacnb4 directly couples electrical activity to gene expression, a process defective in juvenile epilepsy. EMBO J. (0261-4189) 2012 imex curation Tadmouri A., Kiyonaka S., Barbado M., Rousset M., Fablet K., Sawamura S., Bahembera E., Pernet-Gallay K., Arnoult C., Miki T., Sadoul K., Gory-Faure S., Lambrecht C., Lesage F., Akiyama S., Khochbin S., Baulande S., Janssens V., Andrieux A., Dolmetsch R., Ronjat M., Mori Y., De Waard M. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip experimental particp experimental participant identification experimental particp Tadmouri A., Kiyonaka S., Barbado M., Rousset M., Fablet K., Sawamura S., Bahembera E., Pernet-Gallay K., Arnoult C., Miki T., Sadoul K., Gory-Faure S., Lambrecht C., Lesage F., Akiyama S., Khochbin S., Baulande S., Janssens V., Andrieux A., Dolmetsch R., Ronjat M., Mori Y., De Waard M. EMBO J. (0261-4189) 2012 Only protein-protein interactions imex curation Cacnb4 directly couples electrical activity to gene expression, a process defective in juvenile epilepsy. Cacnb4 directly couples electrical activity to gene expression, a process defective in juvenile epilepsy. EMBO J. (0261-4189) 2012 imex curation Tadmouri A., Kiyonaka S., Barbado M., Rousset M., Fablet K., Sawamura S., Bahembera E., Pernet-Gallay K., Arnoult C., Miki T., Sadoul K., Gory-Faure S., Lambrecht C., Lesage F., Akiyama S., Khochbin S., Baulande S., Janssens V., Andrieux A., Dolmetsch R., Ronjat M., Mori Y., De Waard M. Only protein-protein interactions mouse-brain Mus musculus brain brain Brain [cerebrum] anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Tadmouri A., Kiyonaka S., Barbado M., Rousset M., Fablet K., Sawamura S., Bahembera E., Pernet-Gallay K., Arnoult C., Miki T., Sadoul K., Gory-Faure S., Lambrecht C., Lesage F., Akiyama S., Khochbin S., Baulande S., Janssens V., Andrieux A., Dolmetsch R., Ronjat M., Mori Y., De Waard M. EMBO J. (0261-4189) 2012 Only protein-protein interactions imex curation Cacnb4 directly couples electrical activity to gene expression, a process defective in juvenile epilepsy. Cacnb4 directly couples electrical activity to gene expression, a process defective in juvenile epilepsy. EMBO J. (0261-4189) 2012 imex curation Tadmouri A., Kiyonaka S., Barbado M., Rousset M., Fablet K., Sawamura S., Bahembera E., Pernet-Gallay K., Arnoult C., Miki T., Sadoul K., Gory-Faure S., Lambrecht C., Lesage F., Akiyama S., Khochbin S., Baulande S., Janssens V., Andrieux A., Dolmetsch R., Ronjat M., Mori Y., De Waard M. Only protein-protein interactions yeast Saccharomyces cerevisiae Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Tadmouri A., Kiyonaka S., Barbado M., Rousset M., Fablet K., Sawamura S., Bahembera E., Pernet-Gallay K., Arnoult C., Miki T., Sadoul K., Gory-Faure S., Lambrecht C., Lesage F., Akiyama S., Khochbin S., Baulande S., Janssens V., Andrieux A., Dolmetsch R., Ronjat M., Mori Y., De Waard M. EMBO J. (0261-4189) 2012 Only protein-protein interactions imex curation cacb3_rat Voltage-dependent L-type calcium channel subunit beta-3 Cacnb3 Cacnlb3 Calcium channel voltage-dependent subunit beta 3 protein protein rat Rattus norvegicus (Rat) Rat MYDDSYVPGFEDSEAGSADSYTSRPSLDSDVSLEEDRESARREVESQAQQQLERAKHKPVAFAVRTNVSYCGVLDEECPVQGSGVNFEAKDFLHIKEKYSNDWWIGRLVKEGGDIAFIPSPQRLESIRLKQEQKARRSGNPSSLSDIGNRRSPPPSLAKQKQKQAEHVPPYDVVPSMRPVVLVGPSLKGYEVTDMMQKALFDFLKHRFDGRISITRVTADLSLAKRSVLNNPGKRTIIERSSARSSIAEVQSEIERIFELAKSLQLVVLDADTINHPAQLAKTSLAPIIVFVKVSSPKVLQRLIRSRGKSQMKHLTVQMMAYDKLVQCPPESFDVILDENQLDDACEHLAEYLEVYWRATHHPAPGPGMLGPPSAIPGLQNQQLLGERGEEHSPLERDSLMPSDEASESSRQAWTGSSQRSSRHLEEDYADAYQDLYQPHRQHTSGLPSANGHDPQDRLLAQDSEHDHNDRNWQRNRPWPKDSY DIP protein P54287 original sequence version: 89 mint 18815828E9C5C9CA cbx3_mouse Chromobox protein homolog 3 Cbx3 Heterochromatin protein 1 homolog gamma Modifier 2 protein M32 protein protein mouse Mus musculus Mouse MASNKTTLQKMGKKQNGKSKKVEEAEPEEFVVEKVLDRRVVNGKVEYFLKWKGFTDADNTWEPEENLDCPELIEDFLNSQKAGKEKDGTKRKSLSDSESDDSKSKKKRDAADKPRGFARGLDPERIIGATDSSGELMFLMKWKDSDEADLVLAKEANMKCPQIVIAFYEERLTWHSCPEDEAQ DIP protein P23198 original sequence version: 123 DIP protein P23198 original sequence version: 121 DIP protein P23198 original sequence version: 111 2FF3AD53B883E27E pp2ab_mouse Serine/threonine-protein phosphatase 2A catalytic subunit beta isoform Ppp2cb protein protein mouse Mus musculus Mouse MDDKAFTKELDQWVEQLNECKQLNENQVRTLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFRIGGKSPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYPERITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDYLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHVTRRTPDYFL PP2A can modulate the activity of phosphorylase B kinase casein kinase 2, mitogen-stimulated S6 kinase, and MAP-2 kinase mint homomint 51DA9EB0633FC191 cacb4_mouse Voltage-dependent L-type calcium channel subunit beta-4 Cacnb4 Cacnlb4 Calcium channel voltage-dependent subunit beta 4 protein protein mouse Mus musculus Mouse MSSSYGKNGAADGPHSPSSQVARGTTTRRSRLKRSDGSTTSTSFILRQGSADSYTSRPSDSDVSLEEDREAIRQEREQQAAIQLERAKSKPVAFAVKTNVSYCGALDEDVPVPSTAISFDAKDFLHIKEKYNNDWWIGRLVKEGCEIGFIPSPLRLENIRIQQEQKRGRFHGGKSSGNSSSSLGEMVSGTFRATPTTTAKQKQKVTEHIPPYDVVPSMRPVVLVGPSLKGYEVTDMMQKALFDFLKHRFDGRISITRVTADISLAKRSVLNNPSKRAIIERSNTRSSLAEVQSEIERIFELARSLQLVVLDADTINHPAQLIKTSLAPIIVHVKVSSPKVLQRLIKSRGKSQSKHLNVQLVAADKLAQCPPEMFDVILDENQLEDACEHLGEYLEAYWRATHTSSSTPMTPLLGRNVGSTALSPYPTAISGLQSQRMRHSNHSTENSPIERRSLMTSDENYHNERARKSRNRLSSSSQHSRDHYPLVEEDYPDSYQDTYKPHRNRGSPGGCSHDSRHRL 663A88C7BCF7E90C h4_mouse Histone H4 H4c11 H4c12 H4c14 H4c2 H4c3 H4c4 H4c6 H4c8 H4c9 H4f16 Hist1h4a Hist4h4 Hist1h4b Hist1h4c Hist1h4d Hist1h4f Hist1h4h Hist1h4i Hist1h4j Hist1h4k Hist2h4a Hist2h4 Hist1h4m H4-53 H4-12 H4c1 protein protein mouse Mus musculus Mouse MSGRGKGGKGLGKGGAKRHRKVLRDNIQGITKPAIRRLARRGGVKRISGLIYEETRGVLKVFLENVIRDAVTYTEHAKRKTVTAMDVVYALKRQGRTLYGFGG DIP protein P62806 original sequence version: 108 A9E5DFD3F8B97598 cacb4_rat Voltage-dependent L-type calcium channel subunit beta-4 Calcium channel voltage-dependent subunit beta 4 Cacnb4 protein protein rat Rattus norvegicus (Rat) Rat MSSSYAKNGAADGPHSPSSQVARGTTTRRSRLKRSDGSTTSTSFILRQGSADSYTSRPSDSDVSLEEDREAIRQEREQQAAIQLERAKSKPVAFAVKTNVSYCGALDEDVPVPSTAISFDAKDFLHIKEKYNNDWWIGRLVKEGCEIGFIPSPLRLENIRIQQEQKRGRFHGGKSSGNSSSSLGEMVSGTFRATPTTTAKQKQKVTEHIPPYDVVPSMRPVVLVGPSLKGYEVTDMMQKALFDFLKHRFDGRISITRVTADISLAKRSVLNNPSKRAIIERSNTRSSLAEVQSEIERIFELARSLQLVVLDADTINHPAQLIKTSLAPIIVHVKVSSPKVLQRLIKSRGKSQSKHLNVQLVAADKLAQCPPEMFDVILDENQLEDACEHLGEYLEAYWRATHTSSSTPMTPLLGRNVGSTALSPYPTAISGLQSQRMRHSNHSTENSPIERRSLMTSDENYHNERARKSRNRLSSSSQHSRDHYPLVEEDYPDSYQDTYKPHRNRGSPGGCSHDSRHRL 663B2CFE1CF7E90C d4a055-2 Voltage-dependent L-type calcium channel subunit beta-4 Cacnb4 Calcium channel voltage-dependent subunit beta 4 protein protein rat Rattus norvegicus (Rat) Rat MSSSYAKNGAADGPHSPSSQVARGTTTRRSRLKRSDGSTTSTSFILRQGSADSYTSRPSDSDVSLEEDREAIRQEREQQAAIQLERAKSKPVAFAVKTNVSYCGALDEDVPVPSTAISFDAKDFLHIKEKYNNDWWIGRLVKEGCEIGFIPSPLRLENIRIQQEQKRGRFHGGKSSGNSSSSLGEMVSGTFRATPTTTAKQKQKVTEHIPPYDVVPSMRPVVLVGPSLKGYEVTDMMQKALFDFLKHRFDGRISITRVTADISLAKRSVLNNPSKRAIIERSNTRSSLAEVQSEIERIFELARSLQLVVLDADTINHPAQLIKTSLAPIIVHVKVSSPKVLQRLIKSRGKSQSKHLNVQLVAADKLAQCPPEMFDVILDENQLEDACEHLGEYLEAYWRATHTSSSTPMTPLLGRNVGSTALSPYPTAISGLQSQRMRHSNHSTENSPIERRSLMTSDENYHNERARKSRNRLSSSSQHSRDHYPLVEEDYPDSYQDTYKPHRNRGSPGGCSHDSRHRL 663B2CFE1CF7E90C tha_mouse Thyroid hormone receptor alpha Thra C-erba-alpha Nr1a1 Nuclear receptor subfamily 1 group A member 1 c-erbA-1 c-erbA-alpha protein protein mouse Mus musculus Mouse MEQKPSKVECGSDPEENSARSPDGKRKRKNGQCPLKSSMSGYIPSYLDKDEQCVVCGDKATGYHYRCITCEGCKGFFRRTIQKNLHPTYSCKYDSCCVIDKITRNQCQLCRFKKCIAVGMAMDLVLDDSKRVAKRKLIEQNRERRRKEEMIRSLQQRPEPTPEEWDLIHVATEAHRSTNAQGSHWKQRRKFLPDDIGQSPIVSMPDGDKVDLEAFSEFTKIITPAITRVVDFAKKLPMFSELPCEDQIILLKGCCMEIMSLRAAVRYDPESDTLTLSGEMAVKREQLKNGGLGVVSDAIFELGKSLSAFNLDDTEVALLQAVLLMSTDRSGLLCVDKIEKSQEAYLLAFEHYVNHRKHNIPHFWPKLLMKEREVQSSILYKGAAAEGRPGGSLGVHPEGQQLLGMHVVQGPQVRQLEQQLGEAGSLRGPVLQHQSPKSPQQRLLELLHRSGILHSRAVCGEDDSSEASSLSSSSDTEDTEVCEDQAGKAASP DIP protein P63058 original sequence version: 117 DIP protein P63058 original sequence version: 89 mint Nuclear hormone receptor. High affinity receptor for triiodothyronine 870100FCB5C34A10 q91v89_mouse Serine/threonine-protein phosphatase 2A 56 kDa regulatory subunit Ppp2r5d protein protein mouse Mus musculus Mouse MSYKLKKDKEPSKLAKGTAKPSSSSKDGGGENTDEAQPQPQSQSPSSNKRPSNSTPPPTQLSKIKYSGGPQIVKKERRQSSFPFNLNKNRELQKLPALKDSPTQEREELFIQKLRQCCVLFDFVSDPLSDLKCKEVKRAGLNEMVEYITHSRDVVTEAIYPEAVTMFSVNLFRTLPPSSNPTGAEFDPEEDEPTLEAAWPHLQLVYEFFLRFLESPDFQPNIAKKYIDQKFVLALLDLFDSEDPRERDFLKTILHRIYGKFLGLRAYIRRQINHIFYRFIYETEHHNGIAELLEILGSIINGFALPLKEEHKVFLVRVLLPLHKVKSLSVYHPQLAYCVVQFLEKESSLTEPVIVGLLKFWPKTHSPKEVMFLNELEEILDVIEPSEFSKVMEPLFRQLAKCVSSPHFQVAERALYYWNNEYIMSLISDNAARILPIMFPALYRNSKSHWNKTIHGLIYNALKLFMEMNQKLFDDCTQQYKAEKQKGRFRMKEREEMWQKIEELARLNPQYPMFRAPPPLPPVYSMETETPTAEDIQLLKRTVETEAVQMLKDIKKDKVLLRRKSELPQDVYTIKALEAHKRAEEFLTASQEAL mint homomint 693A73931E4518BE d4a055-1 Voltage-dependent L-type calcium channel subunit beta-4 Cacnb4 Calcium channel voltage-dependent subunit beta 4 protein protein rat Rattus norvegicus (Rat) Rat MYDNLYLHGVEDSEAGSADSYTSRPSDSDVSLEEDREAIRQEREQQAAIQLERAKSKPVAFAVKTNVSYCGALDEDVPVPSTAISFDAKDFLHIKEKYNNDWWIGRLVKEGCEIGFIPSPLRLENIRIQQEQKRGRFHGGKSSGNSSSSLGEMVSGTFRATPTTTAKQKQKVTEHIPPYDVVPSMRPVVLVGPSLKGYEVTDMMQKALFDFLKHRFDGRISITRVTADISLAKRSVLNNPSKRAIIERSNTRSSLAEVQSEIERIFELARSLQLVVLDADTINHPAQLIKTSLAPIIVHVKVSSPKVLQRLIKSRGKSQSKHLNVQLVAADKLAQCPPEMFDVILDENQLEDACEHLGEYLEAYWRATHTSSSTPMTPLLGRNVGSTALSPYPTAISGLQSQRMRHSNHSTENSPIERRSLMTSDENYHNERARKSRNRLSSSSQHSRDHYPLVEEDYPDSYQDTYKPHRNRGSPGGCSHDSRHRL cacnb4-cacnb4-2 2 Beta 4 11 anti tag immunost anti tag immunostaining anti tag immunost experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell binding site binding region binding-associated region binding region range range certain certain sequence position certain myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Beta 4 15 tag fluorescence tag visualisation by fluorescence tag fluorescence experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell binding site binding region binding-associated region binding region certain certain sequence position certain certain certain sequence position certain gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 18 21 colocalization colocalization f3c mint cacnb4-ppp2r5d 2 Beta 4 11 tag fluorescence tag visualisation by fluorescence tag fluorescence experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell binding site binding region binding-associated region binding region deletion analysis deletion analysis range range certain certain sequence position certain gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined B56delta 14 anti tag immunost anti tag immunostaining anti tag immunost experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization mint sf3c sf3d cacnb4-cacnb4-1 1 Beta 4 11 anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region range range certain certain sequence position certain Beta 4 12 anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined D4A055-2:p.Leu125Pro mutation disrupting interaction mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Wed May 02 20:27:09 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu125pro' Tue Jun 30 18:21:11 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu125Pro' binding site binding region binding-associated region binding region range range range range 32 36 physical association physical association mint f3d f3f cacnb4-ppp2r5d-1 5 B56delta 14 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Beta 4 11 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell D4A055:p.Leu125Pro mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain L P Thu Dec 08 14:16:05 GMT 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'leu92pro' Wed May 02 19:57:42 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'leu125pro' Tue Jun 30 15:44:24 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Leu125Pro' fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region deletion analysis deletion analysis range range certain certain sequence position certain physical association physical association mint f4a f4d f4c cacnb3-ppp2r5d 5 B56delta 14 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Beta 3 6 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f4c mint cacnb4-thra 5 TRalpha 13 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Beta 4 11 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell fusion protein fusion protein fusion protein undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f6e mint ppp2r5d-cacnb4 3 B56delta 14 anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Beta 4 11 anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined binding site binding region binding-associated region binding region deletion analysis deletion analysis range range certain certain sequence position certain physical association physical association mint Depolarization-induced interaction between B56δ-myc and β4- EGFP requires external Ca 2+ . f4b f5e sf5 ppp2r5d-cacnb4-1 3 Beta 4 11 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined PP2A 8 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level B56delta 14 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association mint f5b f5g cacnb4-ppp2r5d-2 3 B56delta 14 anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Beta 4 9 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f5f homomint mint Membrane depolarization of HEK293 cells enhances b4-myc interaction with B56d-EGFP, while it does not promote any interaction between b1 481-myc and B56d-EGFP (Figure 5F). cacnb4-thra-1 3 Beta 4 11 anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined TRalpha 13 anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey over-expressed over expressed level over-expressed living cell living cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association f6f mint cacnb4-cbx3 3 Beta 4 11 anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined D4A055:p.Val222Ala mutation disrupting interaction mutation disrupting mutation disrupting interaction mutation disrupting mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain V A Thu Dec 08 14:29:50 GMT 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'val189ala' Wed May 02 15:07:17 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'val222ala' Tue Jun 30 16:13:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Val222Ala' D4A055:p.Pro220Ala mutation disrupting interaction mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength mutation analysis mutation analysis certain certain sequence position certain certain certain sequence position certain P A Thu Dec 08 14:34:16 GMT 2016 This feature has been corrected as a result of our quality control procedures. The original label was 'pro187ala' Wed May 02 14:31:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'pro220ala' Tue Jun 30 16:39:06 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Pro220Ala' HP1 gamma 7 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association mint expression of B56d-EGFP strongly enhances immunoprecipitation of HP1g-EGFP by b4-myc (Figure 7G). Finally, double mutation of the HP1g binding motif of b4 precludes the B56d-dependent interaction of b4 with histone H3 demonstrating that HP1g or HP1g-like proteins are required for b4 association to histones (Figure 7M). f7f f7m cacnb4-hist1h4a 3 Beta 4 11 anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined H4 10 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association mint f7i In HEK293 cells, immunoprecipitation of b4-myc leads to the co-precipitation of all histones (H2B, H3 and H4) (Figure 7I), constitutive of nucleosomes. This is in agreement with the endogenous expression of B56d (Supplementary Figure 3a) and HP1g (Supplementary Figure 3d) in these cells. Expression of B56d further enhances b4-myc/histone interaction (Figure 7I). ppp2r5d-hist1h4a 3 B56delta 14 anti tag western anti tag western blot anti tag western experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell myc tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined H4 10 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level physical association physical association homomint mint B56d-myc expressed together with bL125P-EGFP or b1 481-EGFP mutants, deficient for B56d association, no longer precipitate histone H4 further supporting the B56d dependence of b4/HP1g interaction (Figure 7K). f7k cacnb4-ppp2cb 4 B56delta 14 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level PP2A 8 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level Beta 4 9 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level association association f5a homomint mint