MINT MINT, Dpt of Biology, University of Rome Tor Vergata MINT http://mint.bio.uniroma2.it/mint url>http://mint.bio.uniroma2.it/mint/ https://mint.bio.uniroma2.it MINT-[0-9]+ https://mint.bio.uniroma2.it/index.php/results-interactions/?id=${ac} The Molecular INTeraction database (MINT) is a relational database designed to store interactions between biological molecules. http://mint.bio.uniroma2.it DEAD-box protein DDX3 associates with eIF4F to promote translation of selected mRNAs. DEAD-box protein DDX3 associates with eIF4F to promote translation of selected mRNAs. EMBO J. (0261-4189) 2012 imex curation Soto-Rifo R., Rubilar PS., Limousin T., de Breyne S., Décimo D., Ohlmann T. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Soto-Rifo R., Rubilar PS., Limousin T., de Breyne S., Décimo D., Ohlmann T. EMBO J. (0261-4189) 2012 Only protein-protein interactions imex curation DEAD-box protein DDX3 associates with eIF4F to promote translation of selected mRNAs. DEAD-box protein DDX3 associates with eIF4F to promote translation of selected mRNAs. EMBO J. (0261-4189) 2012 imex curation Soto-Rifo R., Rubilar PS., Limousin T., de Breyne S., Décimo D., Ohlmann T. Only protein-protein interactions in vitro In vitro pull down pull down experimental particp experimental participant identification experimental particp Soto-Rifo R., Rubilar PS., Limousin T., de Breyne S., Décimo D., Ohlmann T. EMBO J. (0261-4189) 2012 Only protein-protein interactions imex curation DEAD-box protein DDX3 associates with eIF4F to promote translation of selected mRNAs. DEAD-box protein DDX3 associates with eIF4F to promote translation of selected mRNAs. EMBO J. (0261-4189) 2012 imex curation Soto-Rifo R., Rubilar PS., Limousin T., de Breyne S., Décimo D., Ohlmann T. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 fluorescence imaging fluorescence microscopy fluorescence imaging experimental particp experimental participant identification experimental particp Soto-Rifo R., Rubilar PS., Limousin T., de Breyne S., Décimo D., Ohlmann T. EMBO J. (0261-4189) 2012 Only protein-protein interactions imex curation if4a1_human Eukaryotic initiation factor 4A-I EIF4A1 EIF4A DDX2A ATP-dependent RNA helicase eIF4A-1 protein protein human Homo sapiens Human MSASQDSRSRDNGPDGMEPEGVIESNWNEIVDSFDDMNLSESLLRGIYAYGFEKPSAIQQRAILPCIKGYDVIAQAQSGTGKTATFAISILQQIELDLKATQALVLAPTRELAQQIQKVVMALGDYMGASCHACIGGTNVRAEVQKLQMEAPHIIVGTPGRVFDMLNRRYLSPKYIKMFVLDEADEMLSRGFKDQIYDIFQKLNSNTQVVLLSATMPSDVLEVTKKFMRDPIRILVKKEELTLEGIRQFYINVEREEWKLDTLCDLYETLTITQAVIFINTRRKVDWLTEKMHARDFTVSAMHGDMDQKERDVIMREFRSGSSRVLITTDLLARGIDVQQVSLVINYDLPTNRENYIHRIGRGGRFGRKGVAINMVTEEDKRTLRDIETFYNTSIEEMPLNVADLI DIP protein P60842 original sequence version: 125 DIP protein P60842 original sequence version: 59 DIP protein P60842 original sequence version: 63 DIP protein P60842 original sequence version: 85 DIP protein P60842 original sequence version: 86 DIP protein P60842 original sequence version: 92 DIP protein P60842-1 original sequence version: 114 DIP protein P60842 original sequence version: 106 6EF89939F3045420 ddx3x_human ATP-dependent RNA helicase DDX3X DDX3X DBX DDX3 DEAD box protein 3, X-chromosomal Helicase-like protein 2 DEAD box, X isoform CAP-Rf protein protein human Homo sapiens Human MSHVAVENALGLDQQFAGLDLNSSDNQSGGSTASKGRYIPPHLRNREATKGFYDKDSSGWSSSKDKDAYSSFGSRSDSRGKSSFFSDRGSGSRGRFDDRGRSDYDGIGSRGDRSGFGKFERGGNSRWCDKSDEDDWSKPLPPSERLEQELFSGGNTGINFEKYDDIPVEATGNNCPPHIESFSDVEMGEIIMGNIELTRYTRPTPVQKHAIPIIKEKRDLMACAQTGSGKTAAFLLPILSQIYSDGPGEALRAMKENGRYGRRKQYPISLVLAPTRELAVQIYEEARKFSYRSRVRPCVVYGGADIGQQIRDLERGCHLLVATPGRLVDMMERGKIGLDFCKYLVLDEADRMLDMGFEPQIRRIVEQDTMPPKGVRHTMMFSATFPKEIQMLARDFLDEYIFLAVGRVGSTSENITQKVVWVEESDKRSFLLDLLNATGKDSLTLVFVETKKGADSLEDFLYHEGYACTSIHGDRSQRDREEALHQFRSGKSPILVATAVAARGLDISNVKHVINFDLPSDIEEYVHRIGRTGRVGNLGLATSFFNERNINITKDLLDLLVEAKQEVPSWLENMAYEHHYKGSSRGRSKSSRFSGGFGARDYRQSSGASSSSFSSSRASSSRSGGGGHGSSRGFGGGGYGGFYNSDGYGGNYNSQGVDWWGN DIP protein O00571 original sequence version: 116 DIP protein O00571 original sequence version: 123 7074D2B8A6EBBF09 pabp1_human Polyadenylate-binding protein 1 PABPC1 PAB1 PABP1 PABPC2 PABP protein protein human Homo sapiens Human MNPSAPSYPMASLYVGDLHPDVTEAMLYEKFSPAGPILSIRVCRDMITRRSLGYAYVNFQQPADAERALDTMNFDVIKGKPVRIMWSQRDPSLRKSGVGNIFIKNLDKSIDNKALYDTFSAFGNILSCKVVCDENGSKGYGFVHFETQEAAERAIEKMNGMLLNDRKVFVGRFKSRKEREAELGARAKEFTNVYIKNFGEDMDDERLKDLFGKFGPALSVKVMTDESGKSKGFGFVSFERHEDAQKAVDEMNGKELNGKQIYVGRAQKKVERQTELKRKFEQMKQDRITRYQGVNLYVKNLDDGIDDERLRKEFSPFGTITSAKVMMEGGRSKGFGFVCFSSPEEATKAVTEMNGRIVATKPLYVALAQRKEERQAHLTNQYMQRMASVRAVPNPVINPYQPAPPSGYFMAAIPQTQNRAAYYPPSQIAQLRPSPRWTAQGARPHPFQNMPGAIRPAAPRPPFSTMRPASSQVPRVMSTQRVANTSTQTMGPRPAAAAAAATPAVRTVPQYKYAAGVRNPQQHLNAQPQVTMQQPAVHVQGQEPLTASMLASAPPQEQKQMLGERLFPLIQAMHPTLAGKITGMLLEIDNSELLHMLESPESLRSKVDEAVAVLQAHQAKEAAQKAVNSATGVPTV DIP protein P11940 original sequence version: 187 DIP protein P11940 original sequence version: 181 DIP protein P11940-1 original sequence version: 136 DIP protein P11940 original sequence version: 148 DIP protein P11940 original sequence version: 172 2EB1B02A346132EE if4e_human Eukaryotic translation initiation factor 4E mRNA cap-binding protein eIF-4F 25 kDa subunit EIF4E EIF4EL1 EIF4F protein protein human Homo sapiens Human MATVEPETTPTPNPPTTEEEKTESNQEVANPEHYIKHPLQNRWALWFFKNDKSKTWQANLRLISKFDTVEDFWALYNHIQLSSNLMPGCDYSLFKDGIEPMWEDEKNKRGGRWLITLNKQQRRSDLDRFWLETLLCLIGESFDDYSDDVCGAVVNVRAKGDKIAIWTTECENREAVTHIGRVYKERLGLPPKIVIGYQSHADTATKSGSTTKNRFVV DIP protein P06730 original sequence version: 164 DIP protein P06730 original sequence version: 127 DIP protein P06730 original sequence version: 126 DIP protein P06730 original sequence version: 135 DIP protein P06730 original sequence version: 151 DIP protein P06730 original sequence version: 155 DIP protein P06730 original sequence version: 160 DIP protein P06730-1 original sequence version: 187 DIP protein P06730 original sequence version: 146 B869B8DE615E699D if4g1_human Eukaryotic translation initiation factor 4 gamma 1 p220 EIF4G1 EIF4G EIF4GI EIF4F protein protein human Homo sapiens Human MNKAPQSTGPPPAPSPGLPQPAFPPGQTAPVVFSTPQATQMNTPSQPRQHFYPSRAQPPSSAASRVQSAAPARPGPAAHVYPAGSQVMMIPSQISYPASQGAYYIPGQGRSTYVVPTQQYPVQPGAPGFYPGASPTEFGTYAGAYYPAQGVQQFPTGVAPTPVLMNQPPQIAPKRERKTIRIRDPNQGGKDITEEIMSGARTASTPTPPQTGGGLEPQANGETPQVAVIVRPDDRSQGAIIADRPGLPGPEHSPSESQPSSPSPTPSPSPVLEPGSEPNLAVLSIPGDTMTTIQMSVEESTPISRETGEPYRLSPEPTPLAEPILEVEVTLSKPVPESEFSSSPLQAPTPLASHTVEIHEPNGMVPSEDLEPEVESSPELAPPPACPSESPVPIAPTAQPEELLNGAPSPPAVDLSPVSEPEEQAKEVTASMAPPTIPSATPATAPSATSPAQEEEMEEEEEEEEGEAGEAGEAESEKGGEELLPPESTPIPANLSQNLEAAAATQVAVSVPKRRRKIKELNKKEAVGDLLDAFKEANPAVPEVENQPPAGSNPGPESEGSGVPPRPEEADETWDSKEDKIHNAENIQPGEQKYEYKSDQWKPLNLEEKKRYDREFLLGFQFIFASMQKPEGLPHISDVVLDKANKTPLRPLDPTRLQGINCGPDFTPSFANLGRTTLSTRGPPRGGPGGELPRGPAGLGPRRSQQGPRKEPRKIIATVLMTEDIKLNKAEKAWKPSSKRTAADKDRGEEDADGSKTQDLFRRVRSILNKLTPQMFQQLMKQVTQLAIDTEERLKGVIDLIFEKAISEPNFSVAYANMCRCLMALKVPTTEKPTVTVNFRKLLLNRCQKEFEKDKDDDEVFEKKQKEMDEAATAEERGRLKEELEEARDIARRRSLGNIKFIGELFKLKMLTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIIKEKKTSSRIRFMLQDVLDLRGSNWVPRRGDQGPKTIDQIHKEAEMEEHREHIKVQQLMAKGSDKRRGGPPGPPISRGLPLVDDGGWNTVPISKGSRPIDTSRLTKITKPGSIDSNNQLFAPGGRLSWGKGSSGGSGAKPSDAASEAARPATSTLNRFSALQQAVPTESTDNRRVVQRSSLSRERGEKAGDRGDRLERSERGGDRGDRLDRARTPATKRSFSKEVEERSRERPSQPEGLRKAASLTEDRDRGRDAVKREAALPPVSPLKAALSEEELEKKSKAIIEEYLHLNDMKEAVQCVQELASPSLLFIFVRHGVESTLERSAIAREHMGQLLHQLLCAGHLSTAQYYQGLYEILELAEDMEIDIPHVWLYLAELVTPILQEGGVPMGELFREITKPLRPLGKAASLLLEILGLLCKSMGPKKVGTLWREAGLSWKEFLPEGQDIGAFVAEQKVEYTLGEESEAPGQRALPSEELNRQLEKLLKEGSSNQRVFDWIEANLSEQQIVSNTLVRALMTAVCYSAIIFETPLRVDVAVLKARAKLLQKYLCDEQKELQALYALQALVVTLEQPPNLLRMFFDALYDEDVVKEDAFYSWESSKDPAEQQGKGVALKSVTAFFKWLREAEEESDHN DIP protein Q04637 original sequence version: 68 DIP protein Q04637 original sequence version: 166 DIP protein Q04637 original sequence version: 160 DIP protein Q04637-1 original sequence version: 94 DIP protein Q04637 original sequence version: 115 DIP protein Q04637 original sequence version: 119 DIP protein Q04637 original sequence version: 146 DIP protein Q04637-1 original sequence version: 148 DIP protein Q04637 original sequence version: 150 324088B60863DA34 if4b_human Eukaryotic translation initiation factor 4B EIF4B protein protein human Homo sapiens Human MAASAKKKNKKGKTISLTDFLAEDGGTGGGSTYVSKPVSWADETDDLEGDVSTTWHSNDDDVYRAPPIDRSILPTAPRAAREPNIDRSRLPKSPPYTAFLGNLPYDVTEESIKEFFRGLNISAVRLPREPSNPERLKGFGYAEFEDLDSLLSALSLNEESLGNRRIRVDVADQAQDKDRDDRSFGRDRNRDSDKTDTDWRARPATDSFDDYPPRRGDDSFGDKYRDRYDSDRYRDGYRDGYRDGPRRDMDRYGGRDRYDDRGSRDYDRGYDSRIGSGRRAFGSGYRRDDDYRGGGDRYEDRYDRRDDRSWSSRDDYSRDDYRRDDRGPPQRPKLNLKPRSTPKEDDSSASTSQSTRAASIFGGAKPVDTAAREREVEERLQKEQEKLQRQLDEPKLERRPRERHPSWRSEETQERERSRTGSESSQTGTSTTSSRNARRRESEKSLENETLNKEEDCHSPTSKPPKPDQPLKVMPAPPPKENAWVKRSSNPPARSQSSDTEQQSPTSGGGKVAPAQPSEEGPGRKDENKVDGMNAPKGQTGNSSRGPGDGGNRDHWKESDRKDGKKDQDSRSAPEPKKPEENPASKFSSASKYAALSVDGEDENEGEDYAE 31CEFEA865FB10D2 ddx3x-eif4e-6 3 DDX3 5 tag fluorescence tag visualisation by fluorescence tag fluorescence experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined EIF4E 7 anti tag immunost anti tag immunostaining anti tag immunost experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization f5a homomint mint we induced an oxidative stress in HeLa cells expressing a GFP DDX3 fusion protein together with different HA-tagged members of the eIF4 group. Confocal microscopy analyses revealed that DDX3 was found together with eIF4F components eIF4E, eIF4G, eIF4A but also eIF4B and PABP in cytoplasmic stress granules, confirming its ability to be associated with the translation initiation machinery under these conditions (Figure 5A). ddx3x-eif4g1 3 EIF4G 8 anti tag immunost anti tag immunostaining anti tag immunost experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined DDX3 5 tag fluorescence tag visualisation by fluorescence tag fluorescence experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization f5a homomint mint we induced an oxidative stress in HeLa cells expressing a GFP DDX3 fusion protein together with different HA-tagged members of the eIF4 group. Confocal microscopy analyses revealed that DDX3 was found together with eIF4F components eIF4E, eIF4G, eIF4A but also eIF4B and PABP in cytoplasmic stress granules, confirming its ability to be associated with the translation initiation machinery under these conditions (Figure 5A). ddx3x-eif4a1 3 DDX3 5 tag fluorescence tag visualisation by fluorescence tag fluorescence experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined EIF4A 4 anti tag immunost anti tag immunostaining anti tag immunost experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization f5a homomint mint we induced an oxidative stress in HeLa cells expressing a GFP DDX3 fusion protein together with different HA-tagged members of the eIF4 group. Confocal microscopy analyses revealed that DDX3 was found together with eIF4F components eIF4E, eIF4G, eIF4A but also eIF4B and PABP in cytoplasmic stress granules, confirming its ability to be associated with the translation initiation machinery under these conditions (Figure 5A). ddx3x-eif4b 3 EIF4B 9 anti tag immunost anti tag immunostaining anti tag immunost experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined DDX3 5 tag fluorescence tag visualisation by fluorescence tag fluorescence experimental particp experimental participant identification experimental particp unspecified role unspecified role neutral component neutral component over-expressed over expressed level over-expressed fixed cell fixed cell gfp tag gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization f5a homomint mint we induced an oxidative stress in HeLa cells expressing a GFP DDX3 fusion protein together with different HA-tagged members of the eIF4 group. Confocal microscopy analyses revealed that DDX3 was found together with eIF4F components eIF4E, eIF4G, eIF4A but also eIF4B and PABP in cytoplasmic stress granules, confirming its ability to be associated with the translation initiation machinery under these conditions (Figure 5A). ddx3x-eif4g1-1 1 EIF4G 8 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey living cell living cell physiological level physiological level endogenous level endogenous endogenous endogenous level DDX3 5 western blot western blot Immuno blot Immuno blot experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait over-expressed over expressed level over-expressed living cell living cell ha tag ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association homomint mint By performing immunoprecipitation from HeLa cells expressing HA DDX3, we observed that eIF4G and PABP but not other members of eIF4F were associated with DDX3 (Figure 5B). f5B ddx3x-eif4g1-2 2 DDX3 5 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined EIF4G 8 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated binding site binding region binding-associated region binding region deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain direct interaction direct interaction homomint mint Sf5 Then, we wanted to determine whether interaction between DDX3 and eIF4G was direct or mediated by PABP. For this, we have carried out a GST-pull down assay in the rabbit reticulocyte lysate using in-vitro translated fragments of eIF4G and recombinant GSTDDX3 (Supplementary Figure 5). These data reveal that DDX3 interacts both with PABP and with the middle domain of eIF4G (682 1086) but not with the amino-terminus of eIF4G (1 681). ddx3x-pabpc1-5 2 DDX3 5 predetermined predetermined participant predetermined experimental particp experimental participant identification experimental particp unspecified role unspecified role bait bait purified purified gst tag gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined PABP 6 weight autoradiogra molecular weight estimation by autoradiography weight autoradiogra experimental particp experimental participant identification experimental particp unspecified role unspecified role prey prey in vitro translated in vitro translated protein in vitro translated direct interaction direct interaction homomint mint Sf5 Then, we wanted to determine whether interaction between DDX3 and eIF4G was direct or mediated by PABP. For this, we have carried out a GST-pull down assay in the rabbit reticulocyte lysate using in-vitro translated fragments of eIF4G and recombinant GSTDDX3 (Supplementary Figure 5). These data reveal that DDX3 interacts both with PABP and with the middle domain of eIF4G (682 1086) but not with the amino-terminus of eIF4G (1 681).