UniProt UniProt http://www.uniprot.org Members of the NODE (Nanog and Oct4-associated deacetylase) complex and SOX-2 promote the initiation of a natural cellular reprogramming event in vivo. Members of the NODE (Nanog and Oct4-associated deacetylase) complex and SOX-2 promote the initiation of a natural cellular reprogramming event in vivo. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2012 imex curation Kagias K., Ahier A., Fischer N., Jarriault S. sophie@igbmc.fr 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Kagias K., Ahier A., Fischer N., Jarriault S. sophie@igbmc.fr Accepted 2014-FEB-07 by ORCHARD Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2012 imex curation Members of the NODE (Nanog and Oct4-associated deacetylase) complex and SOX-2 promote the initiation of a natural cellular reprogramming event in vivo. Members of the NODE (Nanog and Oct4-associated deacetylase) complex and SOX-2 promote the initiation of a natural cellular reprogramming event in vivo. Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2012 imex curation Kagias K., Ahier A., Fischer N., Jarriault S. sophie@igbmc.fr 18-Feb-2014: Contacted by IntAct-Help. Only protein-protein interactions imex curation human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 confocal microscopy confocal microscopy tag fluorescence tag visualisation by fluorescence tag fluorescence Kagias K., Ahier A., Fischer N., Jarriault S. sophie@igbmc.fr Accepted 2014-FEB-07 by ORCHARD Proc. Natl. Acad. Sci. U.S.A. (0027-8424) 2012 imex curation sox2_caeel Transcription factor sox-2 sox-2 K08A8.2 protein protein caeel Caenorhabditis elegans MMMDPDSAKMQDYSGWSFGFHMPPTSSTNLLPQSMPDDLSNGPDSPDSTGKDGKKNDDRVKRPMNAFMVWSRGQRKKMALENPKMHNSEISKRLGTEWKMLSEQEKRPFIDEAKRLRAIHMKEHPDYKYRPRRKTKSINKKNGAPIPFGNLDTKTPSYPTLTTNWNATNQYIDQFRFAPYPTTTVMDQIPFSLTYPVHSVPTDNSSPSQFQPSPMSTNFAGSYLTPKSESSPVGSDSTVGTVDSSQFRAYYDHTKDQMMYPYSIELTHAQNIQNALSQSHVTS DIP protein Q21305 original sequence version: 109 71F46FD3BDDAAFA4 g5eff4_caeel sem-4 F15C11.1 CELE_F15C11.1 protein protein caeel Caenorhabditis elegans MNELLAEMAAVSSRRKQSKPRRMSGEGDAMMSPIDLSTKSFDENNCEKGAGGALPLEDRSNILPHFSVPFANPQQFLSLCAQLGNSSSRNVSSTASTTSSCPIQSCSQSFSSPAALTWHVLDAHEDEQEIFSCDVCTTTFSNGQDIREHKCQKTLASRSTSVPPSTIPSSVCFLSTPTTPCLQFSINESIGTSEIREEDEEEDMDVEDGEHVANQLFGHLLQKSDDKSKMASLFNHAFPPFAAFPNMPPPFLMRQPFDPRADVFAAGRHDNDDDWEALMEISTSDEAEKIRALVGDKAVPTTDPNQCILCRRVLSCKSALQMHYRTHTGERPFKCKICQRAFTTKGNLKTHMGVHRSKHSFRGLPISLPPQLAAMHQHQHQIAPPQRIHIHNPPTSAASAAAAVAQIQASQQCPICQQRFLNAGELAVHITEHRNSLTQPPRVMPTPTTTRVQTFPFVPFFTTPPSLNATDMSTQFNLANILSAQLKNDSSPNTDTSSVEEKITRDDPPKMASLSPSNSSDSSSSVRQDILESSEFEEKLKKLEEPPILEQQVSTTPNPKNENPLLAMQKMWAETEPPPPRQMPVLSKHQCGVCFKHFSSSSALQIHMRTHTGDKPFKCDMCGRAFTTRGNLKVHMGTHSWQQSPSRRGRRIFDVASSVTEKPMLQSPILPTSGAPGASPLAMLGPNGLSGLEMMMMLWRTVCSVCQKVCQSPNELEQHLKEHLNNGSSAAPTPLASAATPPPS DIP protein G5EFF4 original sequence version: 31 BCF468C639A30182 egl27_caeel Egg-laying defective protein 27 egl-27 C04A2.3 protein protein caeel Caenorhabditis elegans MSRFDSQCSSEDVNKEDECVPSSSEDSQDGVSSPMENDDEPEFSQKHYDIEPCYYSLTGKSDRNCRGIVYRYRQDSDLKGFQSHDGTLYRLRDSVFVEVSQNEPYVIAAICGFKYTKRDHVVVKLTRYFRADDIPEISLNLMKQERAELEINPHLCPQSLNRELFNSELQITQPVSCLRGKCIVEYVKDVRHARTVADFSLDNDTFFFCLHYNQDSTKLASTHYAIRVGTSFQATLPPMAECSVGDDSDRDELLYRPNSIESGEEEDYIKLARCYRTYTLSGNHMLDSQKNARSLQVSDLLMDEAIIQLHRSGYKIDDALSELNANDIILTTDVDNMTQDDAKKFAKGIKQLGKNFSRIHRELLPHHSREQLVSYYYLWKKTPEATKPKQAARRVNPTSIKRPTKEKVKASRPTSTEYLDFDSASESDVENNGPSGRACHHCYGAESKDWHHANGLLLCTDCRLHYKKYGQLRQIANRPSQVPACLFKRSNSDEEESGVRTRAGKKEQRRRTPSSMSETPDRRSPSTVSNGAPNLTAEETPTKKLNGSVKRAPKRPLHNGVINNVEKSNSSEEPASPTTPPPTLTNGLTNGHGPESSTPNGETISKRMKVEPSYDDDDDEEEGKMTIDEGDDDPMPVLNGFKKEESVEEIKLELNGTIKKENGVETDPTTPTCSMEAENEVCETPAVVSVEIRDETNGETNSDLKDDENVEPDSPEDTFELGSNVEFETKNAMFVRSIVRSCGPRCARTDLIFKIKVGGVWEKSIKEKEERKKVHLQNQRIQDSEKVAIQQNQIKKEQQQSQPTPQQIHQQQAQQNAQHLQQLQQAVMLGHLPPEVLRQMMPPQFGVDPTAILMQQMMAGQQSQGVNAAFQHQMALQQQLEAHQVQFQLMMAHQHQQKMIAEQQQQQRHAAAQQLREREQREQRERERERQHQQQAQQALHQQQQQHAAAAANQLNPAMMQMMALMANSAASQQDIARLMEMAAQQQQQQQQAAQAQAQRDQERERREREAREREAAREREREQAAREAAARDQAAREHAQAVQAAAAAAQQAQALTPDMQHMHLLQQLMLNPALMMQLQQAQAQQQQQQPQVTNPLQMLQHGMAAQSANQAEMMRRIHPEPAMRPQHQ DIP protein Q09228 original sequence version: 116 DIP protein Q09228 original sequence version: 114 6E0F88BBC0AF43E8 hm06_caeel Homeobox protein ceh-6 ceh-6 K02B12.1 protein protein caeel Caenorhabditis elegans MLIPSSSSIPSSLSASASDSEPSSLNGSGISDQNILPNYHLHHHLINENEMEAANYAQVIKPTCEAFQDWPHTPMLYQQPQLHFMLPQHDWAYPHLAQSLPPPHHLTPSTAAVAAATIASQSSIINQTSVVTSTPSCQIKQEVERPEIIQRLMPPWPPAYQFSCDDSGSVSGAGGPHQPLSDISDDSEQTCPDDLEGFAKQFKQRRIKLGYTQADVGVALGTLYGNIFSQTTICRFEALQLSFKNMCKLKPLLFKWLEEADSTTGSPNSTFEKMTGQAGRKRKKRTSIEVNVKSRLEFHFQSNQKPNAQEITQVAMELQLEKEVVRVWFCNRRQKEKRIAPNQYDAPHPMALNNGYPMTADLFPYQAVVNHYTQQSPRQQ DIP protein P20268 original sequence version: 126 46890A452DF90A5E egl_27-sem_4-1 2 4 unspecified role unspecified role neutral component neutral component region yfp tag yellow fluorescent protein tag YFP yellow fluorescent protein yellow fluorescent protein YFP yfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 unspecified role unspecified role neutral component neutral component region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region cfp tag cyan fluorescent protein tag CFP cyan fluorescent protein cyan fluorescent protein CFP cfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined colocalization colocalization Fig. 3B egl_27-sem_4-2 1 5 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 4 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 3B egl_27-ceh_6-1 1 5 unspecified role unspecified role bait bait region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 3D egl_27-sox_2-1 1 3 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association Fig. 3D ceh_6-sox_2-1 1 6 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 3E