IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Roles of the Drosophila LRRK2 homolog in Rab7-dependent lysosomal positioning. Roles of the Drosophila LRRK2 homolog in Rab7-dependent lysosomal positioning. Hum. Mol. Genet. (0964-6906) 2012 imex curation Dodson M.W., Zhang T., Jiang C., Chen S., Guo M. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease I dont see how I can easily separate out these interactions any more. mingfly@ucla.edu 8-May-2012: Contacted by IntAct-Help. Only protein-protein interactions drome-schneider_2 Drosophila melanogaster late embryo cells Fruit fly schneider_2 Established from the late embryo of Drosophila melanogaster Mixture of diploid and tetraploid. Originally had some XY cells now predominantly XX. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Dodson M.W., Zhang T., Jiang C., Chen S., Guo M. Parkinsons - Interactions investigated in the context of Parkinsons disease I dont see how I can easily separate out these interactions any more. mingfly@ucla.edu Accepted 2013-APR-17 by PPORRAS 8-May-2012: Contacted by IntAct-Help. Hum. Mol. Genet. (0964-6906) 2012 Only protein-protein interactions imex curation o76742_drome Rab7 rab7 AAF56218 Dmel_CG5915 DRAB7 DRab7 Dm Rab7 DmRab7 Dmel\CG5915 Drab7 O76742 RAB7 Rab-7 Rab-r7 Rab7A dRab7 drab7 CG5915 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSGRKKSLLKVIILGDSSVGKTSLMNQYVNKRFSNQYKATIGADFCTKEVVVNDRVVTMQIWDTAGQERFQSLGVAFYRGADCCVLVYDVTAPNSFKNLDSWRDEFLIQASPRDPDHFPFVVLGNKVDLDNRQVSTRRAQQWCQSKNDIPYYETSAKEGINVEMAFQVIAKNALELEAEAEVINDFPDQITLGSQNNRPGNPDNCQC FFC76D3630287AEB q9vdj9_drome Non-specific serine/threonine protein kinase Lrrk lrrk Dmel_CG5483 dLRRK2 dLrrk CG5483 CT17358 Dmel\CG5483 LRRK LRRK2 dLRRK Lrrk2 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MEHPKTGTETALEACDYFVDEVIEASSIRDAREEVRQIKHGELRTAVISGDERTVRVLLAALGTERQIIVNMAPSGANTLLFLACQSGYESITQRLLDAGADGRSHAVTKYSPLYAAVHSGHLGIARLMLDHFPELIQQPTVERWLPLHAACINGHIKLLELLISYSYPDYLYQTYRDEEGQWEWRLPFDANAHDVTGQTSLYIASILGNKQLVGVLLKWQLHCRRTLGDSASSVSTPITPTRKRISFGIQAIMSKLHISGESEGPDDLASQESTECQRCPINVNLLCGAARETALLAAVRGGHLDVVQSLLQHGANPNIVAKPVEDHNDPKCCEEIYGLSNVPIAEACKQRSLAMLDLLLKHGARDDNGTAIGMAITCGDEAILSRLLARRVHPDSDYKINKKGLPTPVEVNVFLPSTSNISYSAMFPNNPTIIDWHSMGSSVQLSVVRVPWMVSGVLLLNPKLQSHPRLNEVALTAITRIDFSHNVLTSIPQELFHLVSLRYLNVAQNKITDLPAPIGQTYGCPVLDELFLQDNQLTTLPAAIFHLPALSILDVSNNKLQQLPFDLWRAPKLRELNVAFNLLRDLPVPPMQTSSSLLSLDKLNLQSFEEPPSNKPRNVTQQRLTHRNLWSATLDITDNDMKWQHEQDLGDGKTAGVGSSQLSSLNIANNLFTSIPAALPCLAVNLTRLNMSYNSLRSMGHVTSYPATLKQLDLSHNEISCWPSLPRITESDPHLLCYSCVQLPEGRDDDYKTASSKGSSSSATSFRASVLKSVCRHRRHLRLEALRTLILADNLLTRIQLSTDDATTLFNESEDADWSVVGVNRSKVIFPNLSMLDMTNNCLKEIPASLHELSSLSVLNISGNVNITELPPHLGLLSRLWNLNTRGCLLQEPLRSMIESKKHKTMDIVGYLKSIYEDAQPYARMKLMVVGVAGIGKSTLLDLLRQGAGSGSSSSSHRSRASENHWAKRMGHARSTSRSHRHSSASSANISTVGVDIGTWICEKRKRAPGSHGPVVFRTWDFGGQKEYYATHQYFLSKRSLYLVLWRISDGHKGLAELLQWLGNIQARAPNSPVIIVGTHFDAVGESISPQKAEQLQQLIREKFIAIPDAEKIGLPRVIDSIEISCRTLHNIHLLANIIYDTAMQLRSPGSKEPMLLQKIPASYIALEDIVNVIACNLRAAGRDPVLDGEQYKRLVTEQMRLHNYKSFRDAAELQQATTWCHENGVLLHYDDATLRDYYFLDPQWLCDMLAHVVTVREINPFAPTGVMKLDDLQMLFRSVQVQGNGNRSYIVSLLNKFEVALTWDSRTLLIPSLLPSQEAATPNSGSTVKLSQRSRGRSLGCSVSQEVNLNNLIYEQRSAPSSSSSSASVSQGLRRILLMTYFPSGFWSRLITRILADEQIIEAIRGVYMASQDYADFDLRTSLEQDTQWNLWQTGLALYYGPILIFKIWEVPFQKTERTQPFRTDGNRFKLKQDGIWSDVNLSSSSILEVYFPLYEVNISQEVDDNERQLLAEIRPHMSQVAKLLALTVDHIDLLLEDWYPSLGTRFVHTSEGRFLITRLVLCPRCLWKLQLQQNNEPSDREVPPVGCNRPSRSSRRGAGAYFLHGVGDPGEDGALNVFSAYLNATARRERRSEDSLGAGSDADSGVGPDSAGSSRNTSVDGHPGYHLPDNSNVCYAWMIEECILSVYNQSKISCPVHLEQSMAQLAPDVIFADIPDKHTIPSECIIKGSLLGRGAFGFVFKANCKVRGARSFKPVAMKMLQPVPPGARAKESALMAFKVAVGKWDRDPLQHSCKAYCTARQELAVLLTLKHPNIVPLVGICIKPLALVLELAPLGGLDALLRHYRRSGAHMGPHTFQTLVLQAARAIEYLHRRRIIYRDLKSENVLVWELPQPHTEDSPRNLVHIKIADYGISRQTAPSGAKGFGGTEGFMAPEIIRYNGEEEYTEKVDCFSFGMFIYENISLRQPFEGHESIKECILEGSRPALTQRETQFPTCCLDLMVLCWHEQPRRRPTASQIVSILSAPECIHLLDVVAMPHSEKIVCGVFQSLVGMGDDERCGLELWLPSFGSRIDILDCSPSGSLLQCNSISCSPQPQVAPPKTPENGANSRARSAQRLPKMNMLCCCLVGEAIWMGDVSGNLHAYSTSTYAHLFSYMLDPNIKSAVISLVYMEKIARVAVGTHNGRVFLVDATQMPSNCAFAEGSFVLTEICSGFVLHAACSVVVDGIYELWCGEIAGKINVFPLNENGVSGHQALCHSEEPNLIEDVKVARMCSNESHVFSCLYPGCMVYQWDVISKRIENKLDCSKLLPCSESLQSIAIDEHVNLIKCQISALAAHNSELYIGTTWGCLIVAELHTLRPISVFRPYENEIKSIITLSKDNVPLIATIGRRYRSLISRYVDSAESSTKSSAVSTPTHGAAKSVPPADVDNHIHCLLWRAKHWT A4531E16C103C33F q9v3i2_drome Rab5 Drab5 AAF51265 BAA88244 DRab5 Dm Rab5 DmRab5 Dmel\CG3664 RAB5 RAB5a Rab Rab-5 dRab5 drab5 l(2)k08232 rab5 Dmel_CG3664 CG3664 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MATTPRSGGASGTGTAQRPNGTSQNKSCQFKLVLLGESAVGKSSLVLRFVKGQFHEYQESTIGAAFLTQTICIEDTVVKFEIWDTAGQERYHSLAPMYYRGAQAAIVVYDIQNQDSFQRAKTWVKELHKQASPNIVIALAGNKADLSNIRVVEFDEAKQYAEENGLLFMETSAKTGMNVNDIFLAIAKKLPKNDGANNQGTSIRPTGTETNRPTNNCCK BFA3A28955B7F15D rab5-lrrk 1 4 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role prey prey c_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range physical association physical association 7A The G2014S mutation reduces LRRK binding to Rab7 but not Rab5. rab7-lrrk-1 1 2 unspecified role unspecified role bait bait region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined O76742:p.Thr22Asn mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain T N Wed May 02 15:36:06 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr22asn' Tue Jun 30 18:39:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr22Asn' O76742:p.Gln67Leu mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain Q L Wed May 02 17:29:00 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gln67leu' Tue Jun 30 16:25:19 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gln67Leu' 3 unspecified role unspecified role prey prey c_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range Q9VDJ9:p.Gly1914Ser mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain G S Wed May 02 14:42:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'gly1914ser' Tue Jun 30 15:38:05 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Gly1914Ser' physical association physical association 7A-2/B The G2014S mutation reduces LRRK binding to Rab7 but not Rab5. Lrrk-WT shows a markedly stronger binding with the GTP-binding deficient dominant-negative form of Rab7 (T22N) compared with the constitutively active form of Rab7 (Q67L) that is deficient in GTP hydrolysis. Lrrk-GS appears to bind equally well with both mutant forms of Rab7.