IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Regulation of Drosophila vasa in vivo through paralogous cullin-RING E3 ligase specificity receptors. Regulation of Drosophila vasa in vivo through paralogous cullin-RING E3 ligase specificity receptors. Mol. Cell. Biol. (0270-7306) 2010 imex curation Kugler J.M., Woo J.S., Oh B.H., Lasko P. Paul.Lasko@mcgill.ca 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions drome-ovary Drosophila melanogaster ovary ovary Ovary anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Kugler J.M., Woo J.S., Oh B.H., Lasko P. Paul.Lasko@mcgill.ca Accepted 2013-SEP-06 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation Regulation of Drosophila vasa in vivo through paralogous cullin-RING E3 ligase specificity receptors. Regulation of Drosophila vasa in vivo through paralogous cullin-RING E3 ligase specificity receptors. Mol. Cell. Biol. (0270-7306) 2010 imex curation Kugler J.M., Woo J.S., Oh B.H., Lasko P. Paul.Lasko@mcgill.ca 30-Sept-2013: Contacted by IntAct-Help. Only protein-protein interactions drome-ovary Drosophila melanogaster ovary ovary Ovary anti bait coip anti bait coimmunoprecipitation anti bait coip anti tag western anti tag western blot anti tag western Kugler J.M., Woo J.S., Oh B.H., Lasko P. Paul.Lasko@mcgill.ca Accepted 2013-SEP-06 by ORCHARD 30-Sept-2013: Contacted by IntAct-Help. Mol. Cell. Biol. (0270-7306) 2010 Only protein-protein interactions imex curation fbsp1_drome F-box/SPRY domain-containing protein 1 Fsn CG4643 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MVDPVAALCNYNVLEVIFSYLELDDLSHCSQVCKSWYHFLNDENSDVWRWHCLNKLPKESLKSDLLASVSTYKTKLRAYFHAWSPNDCSRNVYIKPNGFTLHRNPVAQSTDAARGKIGFRHGRHTWEVIWEGPLGTVAVIGISTKEAALQCHGYVALLGSDDQSWGWNLVENHLLHNGDMQGSYPLLNNAPKYQVGERIRVILDCEDNTLSFEKNYEFLGVAFRGLPDKKLYPTVSAVYGNTEVSMVYLGTPLDG 6475FF8BE046A4B3 vasa1_drome ATP-dependent RNA helicase vasa vas Antigen Mab46F11 vasa CG46283 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MSDDWDDEPIVDTRGARGGDWSDDEDTAKSFSGEAEGDGVGGSGGEGGGYQGGNRDVFGRIGGGRGGGAGGYRGGNRDGGGFHGGRREGERDFRGGEGGFRGGQGGSRGGQGGSRGGQGGFRGGEGGFRGRLYENEDGDERRGRLDREERGGERRGRLDREERGGERGERGDGGFARRRRNEDDINNNNNIVEDVERKREFYIPPEPSNDAIEIFSSGIASGIHFSKYNNIPVKVTGSDVPQPIQHFTSADLRDIIIDNVNKSGYKIPTPIQKCSIPVISSGRDLMACAQTGSGKTAAFLLPILSKLLEDPHELELGRPQVVIVSPTRELAIQIFNEARKFAFESYLKIGIVYGGTSFRHQNECITRGCHVVIATPGRLLDFVDRTFITFEDTRFVVLDEADRMLDMGFSEDMRRIMTHVTMRPEHQTLMFSATFPEEIQRMAGEFLKNYVFVAIGIVGGACSDVKQTIYEVNKYAKRSKLIEILSEQADGTIVFVETKRGADFLASFLSEKEFPTTSIHGDRLQSQREQALRDFKNGSMKVLIATSVASRGLDIKNIKHVINYDMPSKIDDYVHRIGRTGRVGNNGRATSFFDPEKDRAIAADLVKILEGSGQTVPDFLRTCGAGGDGGYSNQNFGGVDVRGRGNYVGDATNVEEEEQWD 8617C25CCB3130B9 cul1_drome Cullin homolog 1 Cul1 Lin-19 homolog protein cul-1 lin19 CG1877 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MNRSGNSQTTQKLVNLDDIWSELVEGIMQVFEHEKSLTRSQYMRFYTHVYDYCTSVSAAPSGRSSGKTGGAQLVGKKLYDRLEQFLKSYLSELLTKFKAISGEEVLLSRYTKQWKSYQFSSTVLDGICNYLNRNWVKRECEEGQKGIYKIYRLALVAWKGHLFQVLNEPVTKAVLKSIEEERQGKLINRSLVRDVIECYVELSFNEEDTDAEQQKLSVYKQNFENKFIADTSAFYEKESDAFLSTNTVTEYLKHVENRLEEETQRVRGFNSKNGLSYLHETTADVLKSTCEEVLIEKHLKIFHTEFQNLLNADRNDDLKRMYSLVALSSKNLTDLKSILENHILHQGTEAIAKCCTTDAANDPKTYVQTILDVHKKYNALVLTAFNNDNGFVAALDKACGKFINSNVVTIANSASKSPELLAKYCDLLLKKSSKNPEDKELEDNLNQVMVVFKYIEDKDVFQKYYSKMLAKRLVNHTSASDDAEAMMISKLKQTCGYEYTVKLQRMFQDIGVSKDLNSYFKQYLAEKNLTMEIDFGIEVLSSGSWPFQLSNNFLLPSELERSVRQFNEFYAARHSGRKLNWLYQMCKGELIMNVNRNNSSTYTLQASTFQMSVLLQFNDQLSFTVQQLQDNTQTQQENLIQVLQILLKAKVLTSSDNENSLTPESTVELFLDYKNKKRRININQPLKTELKVEQETVHKHIEEDRKLLIQAAIVRIMKMRKRLNHTNLISEVLNQLSTRFKPKVPVIKKCIDILIEKEYLERMEGHKDTYSYLA DIP protein Q24311 original sequence version: 94 1AF0D035DE85363C q9vaq0_drome CUL5 Cullin5 Dmel\CG1401 cul-5 cul5 Dmel_CG1401 Cul5 Cul-5 Cul-5-RA CG1401 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MLRAIGKRDRDIFEEVWPDKRRIVLKLLRQDTVSQREWQDLFFGVHFVCLWDEKGAAKIYDCLQQDIVEFIVQAQSQVQAQRGEQALLATYIVEWRKFFTQSNYLPLPFRQLEQSPQVKPSSSGSSSSASVSASGASTSAAAAAAATSCSGQAGKSGASTSAAAGASTSAAAAAASTSASTSSAAAAAAASGSGSSSSSTSTSKKNPTEDSPVRKLMLDSWNKHIFHDIKHRLQESAMKIVHAERNGDAYDAQLVVGVRESYVNLSSNAEDKLEIYRENFEMAYLKATVEFYRLKSAEQQQENGVLAYMKYADSKLREEEVRAKRYLEPSSFSILTYTLVNVLIVDHLNSIIAECPALIRDYETERLNLMFRLMDRVMHGVGVEPMMGDLQRHIMSAGLADMLSASEVITQDSEKYVERLLELFNKFSDLVRNAFNDDPRFLTARDIAFKTVVNDTSVFKMELPTSIANRGVKYTAPESKCPELLANYCDMLLRRTPLSKRLTSEQIDARLRDVLLVLKYVNNKDVFMRYHKVHLTRRLILGTSADSEKEEDIVEWLREVGMPADYVNRLARMFQDIKVSEDLNTQFRTSISRHDAINIKILNAGAWARCSERVSVSLPIELEDYIPDVEEFYKKKHSGRKLQWYHHMSNGTITFVNNFGRYDLDVTTFQMAVLFAWNQRQHDKISYENLRLATELPDPELRRTLWSLVAFPKIKKQILLMEPAAISSPKDFAENTMFYINQEFAIVKNGKSQRRGKLNLIGRLQLSTERSQQEDNQSIVQLRILRTQEAIIKIMKVRKRMNNAALQGELIDILKNMFLPSKKMIKEQLEWLIENKYMRRDDDDINMFIYVA 052145CEBF1E0918 gus_drome Protein gustavus gus CG2944 protein protein drome Drosophila melanogaster (Fruit fly) Fruit fly MGQKISGGVKTVSRNDSQSTFKPIIPRELQADFVKPARIDILLDMPPASRDLQLKHSWNSEDRSLNIFVKEDDKLTFHRHPVAQSTDCIRGKVGLTKGLHIWEIYWPTRQRGTHAVVGVCTADAPLHSVGYQSLVGSTEQSWGWDLGRNKLYHDSKNCAGVTYPAILKNDEAFLVPDKFLVALDMDEGTLSFIVDQQYLGIAFRGLRGKKLYPIVSAVWGHCEITMRYIGGLDPEPLPLMDLCRRTIRQKIGRTNLEEHIQQLQLPLSMKTYLLYKNRR DCA657FEEA83D785 vas-fsn 2 3 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait physical association physical association Fig. 1H vas-gus 2 4 unspecified role unspecified role bait bait 7 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 1I, 1J vas-gus-2 2 4 predetermined predetermined participant predetermined anti tag western anti tag western blot anti tag western competitor competitor competitor prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 7 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait association association Fig. 1I The Vas peptide competed with the interaction with a Kd=35nM. vas-fsn-1 2 4 predetermined predetermined participant predetermined anti tag western anti tag western blot anti tag western competitor competitor competitor prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain chemical synthesis Chemical synthesis (Chemical synthesis) 4 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined association association Fig. 1H gus-vas-2 1 7 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey physical association physical association Fig. 2D A Vas peptide containing an intact DINNN motif weakly interferes with this reaction fsn-lin19 1 3 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 5 predetermined predetermined participant predetermined western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey physical association physical association Fig. 3B gus-cul_5 1 7 unspecified role unspecified role bait bait region ha tag ha tag YPYDVPDYA epitope tag YPYDVPDYA epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 6 predetermined predetermined participant predetermined western blot western blot Immuno blot Immuno blot unspecified role unspecified role prey prey physical association physical association Fig. 3B