IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Mutations in the LRRK2 Roc-COR tandem domain link Parkinson's disease to Wnt signalling pathways. Mutations in the LRRK2 Roc-COR tandem domain link Parkinson's disease to Wnt signalling pathways. Hum. Mol. Genet. (0964-6906) 2009 imex curation Sancho R.M., Law B.M., Harvey K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease this is now as ready for checking as it will ever be... The authors confirm that the source of DVL-1 was NM_004421.2/ NP_004412.2 which corresponds to the UniProt protein O14640-2. Amino acid numbering for the LRRK2 ROC_COR domain is derived from Figure 6A. kirsten.harvey@pharmacy.ac.uk 01-March-2013: Contacted by IntAct-Help. Embryonic human brain cDNA library (Clontech) Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Sancho R.M., Law B.M., Harvey K. Parkinsons - Interactions investigated in the context of Parkinsons disease this is now as ready for checking as it will ever be... The authors confirm that the source of DVL-1 was NM_004421.2/ NP_004412.2 which corresponds to the UniProt protein O14640-2. Amino acid numbering for the LRRK2 ROC_COR domain is derived from Figure 6A. kirsten.harvey@pharmacy.ac.uk Accepted 2013-FEB-13 by ORCHARD 01-March-2013: Contacted by IntAct-Help. Embryonic human brain cDNA library (Clontech) Hum. Mol. Genet. (0964-6906) 2009 Only protein-protein interactions imex curation Mutations in the LRRK2 Roc-COR tandem domain link Parkinson's disease to Wnt signalling pathways. Mutations in the LRRK2 Roc-COR tandem domain link Parkinson's disease to Wnt signalling pathways. Hum. Mol. Genet. (0964-6906) 2009 imex curation Sancho R.M., Law B.M., Harvey K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease this is now as ready for checking as it will ever be... The authors confirm that the source of DVL-1 was NM_004421.2/ NP_004412.2 which corresponds to the UniProt protein O14640-2. Amino acid numbering for the LRRK2 ROC_COR domain is derived from Figure 6A. kirsten.harvey@pharmacy.ac.uk 01-March-2013: Contacted by IntAct-Help. Embryonic human brain cDNA library (Clontech) Only protein-protein interactions human-sh_sy5y Homo sapiens SH-SY5Y cell line sh_sy5y Human neuroblastoma cells confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Sancho R.M., Law B.M., Harvey K. Parkinsons - Interactions investigated in the context of Parkinsons disease this is now as ready for checking as it will ever be... The authors confirm that the source of DVL-1 was NM_004421.2/ NP_004412.2 which corresponds to the UniProt protein O14640-2. Amino acid numbering for the LRRK2 ROC_COR domain is derived from Figure 6A. kirsten.harvey@pharmacy.ac.uk Accepted 2013-FEB-13 by ORCHARD 01-March-2013: Contacted by IntAct-Help. Embryonic human brain cDNA library (Clontech) Hum. Mol. Genet. (0964-6906) 2009 Only protein-protein interactions imex curation Mutations in the LRRK2 Roc-COR tandem domain link Parkinson's disease to Wnt signalling pathways. Mutations in the LRRK2 Roc-COR tandem domain link Parkinson's disease to Wnt signalling pathways. Hum. Mol. Genet. (0964-6906) 2009 imex curation Sancho R.M., Law B.M., Harvey K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease this is now as ready for checking as it will ever be... The authors confirm that the source of DVL-1 was NM_004421.2/ NP_004412.2 which corresponds to the UniProt protein O14640-2. Amino acid numbering for the LRRK2 ROC_COR domain is derived from Figure 6A. kirsten.harvey@pharmacy.ac.uk 01-March-2013: Contacted by IntAct-Help. Embryonic human brain cDNA library (Clontech) Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. confocal microscopy confocal microscopy immunocytochemistry immunocytochemistry Sancho R.M., Law B.M., Harvey K. Parkinsons - Interactions investigated in the context of Parkinsons disease this is now as ready for checking as it will ever be... The authors confirm that the source of DVL-1 was NM_004421.2/ NP_004412.2 which corresponds to the UniProt protein O14640-2. Amino acid numbering for the LRRK2 ROC_COR domain is derived from Figure 6A. kirsten.harvey@pharmacy.ac.uk Accepted 2013-FEB-13 by ORCHARD 01-March-2013: Contacted by IntAct-Help. Embryonic human brain cDNA library (Clontech) Hum. Mol. Genet. (0964-6906) 2009 Only protein-protein interactions imex curation Mutations in the LRRK2 Roc-COR tandem domain link Parkinson's disease to Wnt signalling pathways. Mutations in the LRRK2 Roc-COR tandem domain link Parkinson's disease to Wnt signalling pathways. Hum. Mol. Genet. (0964-6906) 2009 imex curation Sancho R.M., Law B.M., Harvey K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease this is now as ready for checking as it will ever be... The authors confirm that the source of DVL-1 was NM_004421.2/ NP_004412.2 which corresponds to the UniProt protein O14640-2. Amino acid numbering for the LRRK2 ROC_COR domain is derived from Figure 6A. kirsten.harvey@pharmacy.ac.uk 01-March-2013: Contacted by IntAct-Help. Embryonic human brain cDNA library (Clontech) Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid predetermined predetermined participant predetermined Sancho R.M., Law B.M., Harvey K. Parkinsons - Interactions investigated in the context of Parkinsons disease this is now as ready for checking as it will ever be... The authors confirm that the source of DVL-1 was NM_004421.2/ NP_004412.2 which corresponds to the UniProt protein O14640-2. Amino acid numbering for the LRRK2 ROC_COR domain is derived from Figure 6A. kirsten.harvey@pharmacy.ac.uk Accepted 2013-FEB-13 by ORCHARD 01-March-2013: Contacted by IntAct-Help. Embryonic human brain cDNA library (Clontech) Hum. Mol. Genet. (0964-6906) 2009 Only protein-protein interactions imex curation Mutations in the LRRK2 Roc-COR tandem domain link Parkinson's disease to Wnt signalling pathways. Mutations in the LRRK2 Roc-COR tandem domain link Parkinson's disease to Wnt signalling pathways. Hum. Mol. Genet. (0964-6906) 2009 imex curation Sancho R.M., Law B.M., Harvey K. Neurodegeneration - Publications depicting interactions involved in neurodegenerative diseases. Parkinsons - Interactions investigated in the context of Parkinsons disease this is now as ready for checking as it will ever be... The authors confirm that the source of DVL-1 was NM_004421.2/ NP_004412.2 which corresponds to the UniProt protein O14640-2. Amino acid numbering for the LRRK2 ROC_COR domain is derived from Figure 6A. kirsten.harvey@pharmacy.ac.uk 01-March-2013: Contacted by IntAct-Help. Embryonic human brain cDNA library (Clontech) Only protein-protein interactions yeasx Saccharomyces cerevisiae (Baker's yeast) Baker's yeast 2 hybrid two hybrid 2H 2h Gal4 transcription regeneration two-hybrid yeast two hybrid 2-hybrid classical two hybrid classical two hybrid 2H two-hybrid 2-hybrid Gal4 transcription regeneration 2h yeast two hybrid Y2H Y-2H 2 hybrid nucleotide sequence nucleotide sequence identification sequence cloning sequence cloning nucleotide sequence Sancho R.M., Law B.M., Harvey K. Parkinsons - Interactions investigated in the context of Parkinsons disease this is now as ready for checking as it will ever be... The authors confirm that the source of DVL-1 was NM_004421.2/ NP_004412.2 which corresponds to the UniProt protein O14640-2. Amino acid numbering for the LRRK2 ROC_COR domain is derived from Figure 6A. kirsten.harvey@pharmacy.ac.uk Accepted 2013-FEB-13 by ORCHARD 01-March-2013: Contacted by IntAct-Help. Embryonic human brain cDNA library (Clontech) Hum. Mol. Genet. (0964-6906) 2009 Only protein-protein interactions imex curation o14640-2 Segment polarity protein dishevelled homolog DVL-1 DVL1 DSH homolog 1 protein protein human Homo sapiens Human MAETKIIYHMDEEETPYLVKLPVAPERVTLADFKNVLSNRPVHAYKFFFKSMDQDFGVVKEEIFDDNAKLPCFNGRVVSWLVLAEGAHSDAGSQGTDSHTDLPPPLERTGGIGDSRPPSFHPNVASSRDGMDNETGTESMVSHRRERARRRNREEAARTNGHPRGDRRRDVGLPPDSASTALSSELESSSFVDSDEDGSTSRLSSSTEQSTSSRLIRKHKRRRRKQRLRQADRASSFSSITDSTMSLNIVTVTLNMERHHFLGISIVGQSNDRGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDVNFENMSNDDAVRVLREIVSQTGPISLTVAKCWDPTPRSYFTVPRADPVRPIDPAAWLSHTAALTGALPRYELEEAPLTVKSDMSAVVRVMQLPDSGLEIRDRMWLKITIANAVIGADVVDWLYTHVEGFKERREARKYASSLLKHGFLRHTVNKITFSEQCYYVFGDLCSNLATLNLNSGSSGTSDQDTLAPLPHPAAPWPLGQGYPYQYPGPPPCFPPAYQDPGFSYGSGSTGSQQSEGSKSSGSTRSSRRAPGREKERRAAGAGGSGSESDHTAPSGVGSSWRERPAGQLSRGSSPRSQASATAPGLPPPHPTTKAYTVVGGPPGGPPVRELAAVPPELTGSRQSFQKAMGNPCEFFVDIM CF31534E784A80A2 dvl2_human Segment polarity protein dishevelled homolog DVL-2 DSH homolog 2 DVL2 protein protein human Homo sapiens Human MAGSSTGGGGVGETKVIYHLDEEETPYLVKIPVPAERITLGDFKSVLQRPAGAKYFFKSMDQDFGVVKEEISDDNARLPCFNGRVVSWLVSSDNPQPEMAPPVHEPRAELAPPAPPLPPLPPERTSGIGDSRPPSFHPNVSSSHENLEPETETESVVSLRRERPRRRDSSEHGAGGHRTGGPSRLERHLAGYESSSTLMTSELESTSLGDSDEEDTMSRFSSSTEQSSASRLLKRHRRRRKQRPPRLERTSSFSSVTDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNDMNFENMSNDDAVRVLRDIVHKPGPIVLTVAKCWDPSPQAYFTLPRNEPIQPIDPAAWVSHSAALTGTFPAYPGSSSMSTITSGSSLPDGCEGRGLSVHTDMASVTKAMAAPESGLEVRDRMWLKITIPNAFLGSDVVDWLYHHVEGFPERREARKYASGLLKAGLIRHTVNKITFSEQCYYVFGDLSGGCESYLVNLSLNDNDGSSGASDQDTLAPLPGATPWPLLPTFSYQYPAPHPYSPQPPPYHELSSYTYGGGSASSQHSEGSRSSGSTRSDGGAGRTGRPEERAPESKSGSGSESEPSSRGGSLRRGGEASGTSDGGPPPSRGSTGGAPNLRAHPGLHPYGPPPGMALPYNPMMVVMMPPPPPPVPPAVQPPGAPPVRDLGSVPPELTASRQSFHMAMGNPSEFFVDVM DIP protein O14641 original sequence version: 150 DIP protein O14641 original sequence version: 113 DIP protein O14641 original sequence version: 124 DIP protein O14641 original sequence version: 154 DIP protein O14641 original sequence version: 159 4BAD95B6C3FE531B lrrk2_human Leucine-rich repeat serine/threonine-protein kinase 2 LRRK2 PARK8 Dardarin protein protein human Homo sapiens Human MASGSCQGCEEDEETLKKLIVRLNNVQEGKQIETLVQILEDLLVFTYSERASKLFQGKNIHVPLLIVLDSYMRVASVQQVGWSLLCKLIEVCPGTMQSLMGPQDVGNDWEVLGVHQLILKMLTVHNASVNLSVIGLKTLDLLLTSGKITLLILDEESDIFMLIFDAMHSFPANDEVQKLGCKALHVLFERVSEEQLTEFVENKDYMILLSALTNFKDEEEIVLHVLHCLHSLAIPCNNVEVLMSGNVRCYNIVVEAMKAFPMSERIQEVSCCLLHRLTLGNFFNILVLNEVHEFVVKAVQQYPENAALQISALSCLALLTETIFLNQDLEEKNENQENDDEGEEDKLFWLEACYKALTWHRKNKHVQEAACWALNNLLMYQNSLHEKIGDEDGHFPAHREVMLSMLMHSSSKEVFQASANALSTLLEQNVNFRKILLSKGIHLNVLELMQKHIHSPEVAESGCKMLNHLFEGSNTSLDIMAAVVPKILTVMKRHETSLPVQLEALRAILHFIVPGMPEESREDTEFHHKLNMVKKQCFKNDIHKLVLAALNRFIGNPGIQKCGLKVISSIVHFPDALEMLSLEGAMDSVLHTLQMYPDDQEIQCLGLSLIGYLITKKNVFIGTGHLLAKILVSSLYRFKDVAEIQTKGFQTILAILKLSASFSKLLVHHSFDLVIFHQMSSNIMEQKDQQFLNLCCKCFAKVAMDDYLKNVMLERACDQNNSIMVECLLLLGADANQAKEGSSLICQVCEKESSPKLVELLLNSGSREQDVRKALTISIGKGDSQIISLLLRRLALDVANNSICLGGFCIGKVEPSWLGPLFPDKTSNLRKQTNIASTLARMVIRYQMKSAVEEGTASGSDGNFSEDVLSKFDEWTFIPDSSMDSVFAQSDDLDSEGSEGSFLVKKKSNSISVGEFYRDAVLQRCSPNLQRHSNSLGPIFDHEDLLKRKRKILSSDDSLRSSKLQSHMRHSDSISSLASEREYITSLDLSANELRDIDALSQKCCISVHLEHLEKLELHQNALTSFPQQLCETLKSLTHLDLHSNKFTSFPSYLLKMSCIANLDVSRNDIGPSVVLDPTVKCPTLKQFNLSYNQLSFVPENLTDVVEKLEQLILEGNKISGICSPLRLKELKILNLSKNHISSLSENFLEACPKVESFSARMNFLAAMPFLPPSMTILKLSQNKFSCIPEAILNLPHLRSLDMSSNDIQYLPGPAHWKSLNLRELLFSHNQISILDLSEKAYLWSRVEKLHLSHNKLKEIPPEIGCLENLTSLDVSYNLELRSFPNEMGKLSKIWDLPLDELHLNFDFKHIGCKAKDIIRFLQQRLKKAVPYNRMKLMIVGNTGSGKTTLLQQLMKTKKSDLGMQSATVGIDVKDWPIQIRDKRKRDLVLNVWDFAGREEFYSTHPHFMTQRALYLAVYDLSKGQAEVDAMKPWLFNIKARASSSPVILVGTHLDVSDEKQRKACMSKITKELLNKRGFPAIRDYHFVNATEESDALAKLRKTIINESLNFKIRDQLVVGQLIPDCYVELEKIILSERKNVPIEFPVIDRKRLLQLVRENQLQLDENELPHAVHFLNESGVLLHFQDPALQLSDLYFVEPKWLCKIMAQILTVKVEGCPKHPKGIISRRDVEKFLSKKRKFPKNYMSQYFKLLEKFQIALPIGEEYLLVPSSLSDHRPVIELPHCENSEIIIRLYEMPYFPMGFWSRLINRLLEISPYMLSGRERALRPNRMYWRQGIYLNWSPEAYCLVGSEVLDNHPESFLKITVPSCRKGCILLGQVVDHIDSLMEEWFPGLLEIDICGEGETLLKKWALYSFNDGEEHQKILLDDLMKKAEEGDLLVNPDQPRLTIPISQIAPDLILADLPRNIMLNNDELEFEQAPEFLLGDGSFGSVYRAAYEGEEVAVKIFNKHTSLRLLRQELVVLCHLHHPSLISLLAAGIRPRMLVMELASKGSLDRLLQQDKASLTRTLQHRIALHVADGLRYLHSAMIIYRDLKPHNVLLFTLYPNAAIIAKIADYGIAQYCCRMGIKTSEGTPGFRAPEVARGNVIYNQQADVYSFGLLLYDILTTGGRIVEGLKFPNEFDELEIQGKLPDPVKEYGCAPWPMVEKLIKQCLKENPQERPTSAQVFDILNSAELVCLTRRILLPKNVIVECMVATHHNSRNASIWLGCGHTDRGQLSFLDLNTEGYTSEEVADSRILCLALVHLPVEKESWIVSGTQSGTLLVINTEDGKKRHTLEKMTDSVTCLYCNSFSKQSKQKNFLLVGTADGKLAIFEDKTVKLKGAAPLKILNIGNVSTPLMCLSESTNSTERNVMWGGCGTKIFSFSNDFTIQKLIETRTSQLFSYAAFSDSNIITVVVDTALYIAKQNSPVVEVWDKKTEKLCGLIDCVHFLREVMVKENKESKHKMSYSGRVKTLCLQKNTALWIGTGGGHILLLDLSTRRLIRVIYNFCNSVRVMMTAQLGSLKNVMLVLGYNRKNTEGTQKQKEIQSCLTVWDINLPHEVQNLEKHIEVRKELAEKMRRTSVE DIP protein Q5S007 original sequence version: 36 DIP protein Q5S007 original sequence version: 68 DIP protein Q5S007 original sequence version: 64 26142A0CECBBC3F4 dvl3_human Segment polarity protein dishevelled homolog DVL-3 DVL3 KIAA0208 DSH homolog 3 protein protein human Homo sapiens Human MGETKIIYHLDGQETPYLVKLPLPAERVTLADFKGVLQRPSYKFFFKSMDDDFGVVKEEISDDNAKLPCFNGRVVSWLVSAEGSHPDPAPFCADNPSELPPPMERTGGIGDSRPPSFHPHAGGGSQENLDNDTETDSLVSAQRERPRRRDGPEHATRLNGTAKGERRREPGGYDSSSTLMSSELETTSFFDSDEDDSTSRFSSSTEQSSASRLMRRHKRRRRKQKVSRIERSSSFSSITDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNEINFENMSNDDAVRVLREIVHKPGPITLTVAKCWDPSPRGCFTLPRSEPIRPIDPAAWVSHTAAMTGTFPAYGMSPSLSTITSTSSSITSSIPDTERLDDFHLSIHSDMAAIVKAMASPESGLEVRDRMWLKITIPNAFIGSDVVDWLYHNVEGFTDRREARKYASNLLKAGFIRHTVNKITFSEQCYYIFGDLCGNMANLSLHDHDGSSGASDQDTLAPLPHPGAAPWPMAFPYQYPPPPHPYNPHPGFPELGYSYGGGSASSQHSEGSRSSGSNRSGSDRRKEKDPKAGDSKSGGSGSESDHTTRSSLRGPRERAPSERSGPAASEHSHRSHHSLASSLRSHHTHPSYGPPGVPPLYGPPMLMMPPPPAAMGPPGAPPGRDLASVPPELTASRQSFRMAMGNPSEFFVDVM DIP protein Q92997 original sequence version: 110 B1A55EBF9507D06E lrrk2-dvl2 5 7 unspecified role unspecified role prey prey regions sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 8 unspecified role unspecified role bait bait roc_cor_domains sufficient to bind sufficient binding region sufficient to bind range range range range physical association physical association no figure lrrk2-dvl2-1 5 8 unspecified role unspecified role bait bait roc_cor_domains sufficient to bind sufficient binding region sufficient to bind range range range range 9 unspecified role unspecified role prey prey regions sufficient to bind sufficient binding region sufficient to bind undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association no figure lrrk2-dvl2-2 4 8 unspecified role unspecified role bait bait roc_cor_domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range Q5S007:p.Tyr1699Cys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain Y C Wed May 02 18:43:31 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1699cys' Tue Jun 30 19:31:27 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr1699Cys' Q5S007:p.Arg1514Gln mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain R Q Wed May 02 16:39:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1514gln' Tue Jun 30 19:14:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg1514Gln' 7 unspecified role unspecified role prey prey delta_dix sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 1A, 4J, 6B/E An intact ROC-COR tandem domain is required for LRRK2-DVL interactions. lrrk2-dvl2-2 4 8 unspecified role unspecified role bait bait roc_cor_domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range Q5S007:p.Tyr1699Cys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain Y C Wed May 02 15:02:50 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1699cys' Tue Jun 30 19:41:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr1699Cys' 9 unspecified role unspecified role prey prey region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 1A, 6B/F lrrk2-dvl3 4 8 unspecified role unspecified role bait bait Q5S007:p.Arg1728His mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain R H Wed May 02 15:32:45 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1728his' Tue Jun 30 19:46:28 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg1728His' Q5S007:p.Arg1441His mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain R H Wed May 02 17:38:47 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1441his' Tue Jun 30 18:04:21 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg1441His' Q5S007:p.Tyr1699Cys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain Y C Wed May 02 15:34:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1699cys' Tue Jun 30 16:35:54 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr1699Cys' Q5S007:p.Arg1728Leu mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain R L Wed May 02 17:05:57 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1728leu' Tue Jun 30 15:57:20 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg1728Leu' Q5S007:p.Arg1441Cys mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain R C Wed May 02 18:21:18 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1441cys' Tue Jun 30 18:03:48 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg1441Cys' Q5S007:p.Arg1441Gly mutation increasing mutation increasing interaction mutation increasing certain certain sequence position certain certain certain sequence position certain R G Wed May 02 17:24:07 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg1441gly' Tue Jun 30 18:32:44 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg1441Gly' 6 unspecified role unspecified role prey prey dep_domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 1A, 2A, 4J,6B/C Substitutions at residues R1441 and R1728 appear to strengthen the LRRK2–DVL1 interaction, specifically for full-length DVL1 only. dvl1-lrrk2-5 1 6 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range dep_domain required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey roc_cor_domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range physical association physical association 1B, 2B, 4K. dvl2-lrrk2 1 8 unspecified role unspecified role prey prey n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range roc_cor_domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range 7 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range delta_dix sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain physical association physical association 1B, 4K. dvl3-lrrk2-1 1 9 unspecified role unspecified role bait bait n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role prey prey n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range roc_cor_domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range physical association physical association 1B, 4K. dvl3-lrrk2 3 9 unspecified role unspecified role neutral component neutral component n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role neutral component neutral component c_terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization 3 G-I dvl1-lrrk2-1 3 6 unspecified role unspecified role neutral component neutral component n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role neutral component neutral component c_terminus tap tagged calmodulin binding peptide plus protein a tag CBP-ProtA tagged CBP-ProtA tagged tap tagged c-term range C-terminal range c-term range c-term range C-terminal range c-term range colocalization colocalization 3 D-F dvl1-lrrk2-2 2 6 unspecified role unspecified role neutral component neutral component n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role neutral component neutral component n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range colocalization colocalization 3 O-Q, 7P/T, 8. dvl1-lrrk2-3 3 6 unspecified role unspecified role neutral component neutral component n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range 8 unspecified role unspecified role neutral component neutral component n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range roc_cor_domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range colocalization colocalization 4 A-F dvl1-lrrk2-4 3 8 unspecified role unspecified role neutral component neutral component n_terminus myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range 6 unspecified role unspecified role neutral component neutral component n_terminus flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged n-term range n-terminal range n-term range n-term range n-terminal range n-term range delta_dix sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain colocalization colocalization 4 G-I Deletion of the N-terminal DIX region resulted in the loss of cytoplasmic DVL1 aggregates and the redistribution of FLAG-DVL1ΔDIX and myc-LRRK2 throughout the cytoplasm with some enrichment at the cell membrane. lrrk2-dvl1 4 6 unspecified role unspecified role prey prey delta_dix required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain 8 unspecified role unspecified role bait bait Q5S007:p.Tyr1699Cys mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing certain certain sequence position certain certain certain sequence position certain Y C Wed May 02 19:43:14 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'tyr1699cys' Tue Jun 30 18:47:00 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Tyr1699Cys' roc_cor_domains sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain range range physical association physical association 6D deletion of the DVL1 DIX domain (DVL1ΔDIX) increases the strength of the Roc-COR-DVL1 interaction. An intact ROC-COR tandem domain is required for LRRK2-DVL interactions. Substitutions at residues R1441 and R1728 appear to strengthen the LRRK2–DVL1 interaction, specifically for full-length DVL1 only.