UniProt UniProt http://www.uniprot.org A shared interface mediates paramyxovirus interference with antiviral RNA helicases MDA5 and LGP2. A shared interface mediates paramyxovirus interference with antiviral RNA helicases MDA5 and LGP2. J. Virol. (0022-538X) 2009 mimix curation Parisien J.P., Bamming D., Komuro A., Ramachandran A., Rodriguez J.J., Barber G., Wojahn R.D., Horvath C.M. Mapping of viral proteins to UniProt: Measles virus (strain Edmonston) (MeV) according Ref. 17, pmid 18579593; Mumps virus (strain Enders) (MuV) according Ref. 26 leading to (Ref 60 therein) pmid 12743296. For PIV5 (HPIV-5/SV5), HPIV-2, Hendra and Nipah proteins the UniProt virus reference strains have been used. DDX58 was mapped to human origin by sequence length, as the same authors also created the IFIH1 and LGP" constructs using the same PCR reaction these have also been mapped to human. horvath@northwestern.edu 8-May-2012: Contacted by IntAct-Help. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Parisien J.P., Bamming D., Komuro A., Ramachandran A., Rodriguez J.J., Barber G., Wojahn R.D., Horvath C.M. Mapping of viral proteins to UniProt: Measles virus (strain Edmonston) (MeV) according Ref. 17, pmid 18579593; Mumps virus (strain Enders) (MuV) according Ref. 26 leading to (Ref 60 therein) pmid 12743296. For PIV5 (HPIV-5/SV5), HPIV-2, Hendra and Nipah proteins the UniProt virus reference strains have been used. DDX58 was mapped to human origin by sequence length, as the same authors also created the IFIH1 and LGP" constructs using the same PCR reaction these have also been mapped to human. horvath@northwestern.edu Accepted 2013-APR-26 by ORCHARD 8-May-2012: Contacted by IntAct-Help. J. Virol. (0022-538X) 2009 mimix curation A shared interface mediates paramyxovirus interference with antiviral RNA helicases MDA5 and LGP2. A shared interface mediates paramyxovirus interference with antiviral RNA helicases MDA5 and LGP2. J. Virol. (0022-538X) 2009 mimix curation Parisien J.P., Bamming D., Komuro A., Ramachandran A., Rodriguez J.J., Barber G., Wojahn R.D., Horvath C.M. Mapping of viral proteins to UniProt: Measles virus (strain Edmonston) (MeV) according Ref. 17, pmid 18579593; Mumps virus (strain Enders) (MuV) according Ref. 26 leading to (Ref 60 therein) pmid 12743296. For PIV5 (HPIV-5/SV5), HPIV-2, Hendra and Nipah proteins the UniProt virus reference strains have been used. DDX58 was mapped to human origin by sequence length, as the same authors also created the IFIH1 and LGP" constructs using the same PCR reaction these have also been mapped to human. horvath@northwestern.edu 8-May-2012: Contacted by IntAct-Help. human-293t Homo sapiens 293 cells transformed with SV40 large T antigen 293t 293 cells expressing SV40 large T antigen. The original designation of this cell line was 293tsA1609neo. It is not in Cabri,ATCC or HyperCLDB. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Parisien J.P., Bamming D., Komuro A., Ramachandran A., Rodriguez J.J., Barber G., Wojahn R.D., Horvath C.M. Mapping of viral proteins to UniProt: Measles virus (strain Edmonston) (MeV) according Ref. 17, pmid 18579593; Mumps virus (strain Enders) (MuV) according Ref. 26 leading to (Ref 60 therein) pmid 12743296. For PIV5 (HPIV-5/SV5), HPIV-2, Hendra and Nipah proteins the UniProt virus reference strains have been used. DDX58 was mapped to human origin by sequence length, as the same authors also created the IFIH1 and LGP" constructs using the same PCR reaction these have also been mapped to human. horvath@northwestern.edu Accepted 2013-APR-26 by ORCHARD 8-May-2012: Contacted by IntAct-Help. J. Virol. (0022-538X) 2009 mimix curation dhx58_human Probable ATP-dependent RNA helicase DHX58 Probable ATP-dependent helicase LGP2 Protein D11Lgp2 homolog DHX58 D11LGP2E LGP2 RIG-I-like receptor LGP2 RIG-I-like receptor 3 protein protein human Homo sapiens Human MELRSYQWEVIMPALEGKNIIIWLPTGAGKTRAAAYVAKRHLETVDGAKVVVLVNRVHLVTQHGEEFRRMLDGRWTVTTLSGDMGPRAGFGHLARCHDLLICTAELLQMALTSPEEEEHVELTVFSLIVVDECHHTHKDTVYNVIMSQYLELKLQRAQPLPQVLGLTASPGTGGASKLDGAINHVLQLCANLDTWCIMSPQNCCPQLQEHSQQPCKQYNLCHRRSQDPFGDLLKKLMDQIHDHLEMPELSRKFGTQMYEQQVVKLSEAAALAGLQEQRVYALHLRRYNDALLIHDTVRAVDALAALQDFYHREHVTKTQILCAERRLLALFDDRKNELAHLATHGPENPKLEMLEKILQRQFSSSNSPRGIIFTRTRQSAHSLLLWLQQQQGLQTVDIRAQLLIGAGNSSQSTHMTQRDQQEVIQKFQDGTLNLLVATSVAEEGLDIPHCNVVVRYGLLTNEISMVQARGRARADQSVYAFVATEGSRELKRELINEALETLMEQAVAAVQKMDQAEYQAKIRDLQQAALTKRAAQAAQRENQRQQFPVEHVQLLCINCMVAVGHGSDLRKVEGTHHVNVNPNFSNYYNVSRDPVVINKVFKDWKPGGVISCRNCGEVWGLQMIYKSVKLPVLKVRSMLLETPQGRIQAKKWSRVPFSVPDFDFLQHCAENLSDLSLD DIP protein Q96C10 original sequence version: 101 859E1749C7313D06 v_hendh Non-structural protein V P/V/C protein protein hendh Hendra virus (isolate Horse/Autralia/Hendra/1994) MDKLDLVNDGLDIIDFIQKNQKEIQKTYGRSSIQQPSTKDRTRAWEDFLQSTSGEHEQAEGGMPKNDGGTEGRNVEDLSSVTSSDGTIGQRVSNTRAWAEDPDDIQLDPMVTDVVYHDHGGECTGHGPSSSPERGWSYHMSGTHDGNVRAVPDTKVLPNAPKTTVPEEVREIDLIGLEDKFASAGLNPAAVPFVPKNQSTPTEEPPVIPEYYYGSGRRGDLSKSPPRGNVNLDSIKIYTSDDEDENQLEYEDEFAKSSSEVVIDTTPEDNDSINQEEVVGDPSDQGLEHPFPLGKFPEKEETPDVRRKDSLMQDSCKRGGVPKRLPMLSEEFECSGSDDPIIQELEREGSHPGGSLRLREPPQSSGNSRNQPDRQLKTGDAASPGGVQRPGTPMPKSRIMPIKKGHRREVSICWDGRRAWVEEWCNPVCSRITPQPRKQECYCGECPTECSQCCHEE 15C63B4FF638587D v_mumpe Non-structural protein V P/V Non-structural protein NS1 protein protein mumpe Mumps virus (strain Enders) MDQFIKQDETGDLIETGMNVANHFLSAPIQGTNSLSKASIIPGVAPVLIGNPEQKNIQHPTASHQGSKSKGRGSGVRSIIVPPSEAGNGGTQIPEPLFAQTGQGGIVTTVYQDPTIQPTGSYRSVELTKIGKERMINRFVEKPRISTPVTEFKRGAGSGCSRPDNPRGGHRREWSLSWVQGEVRVFEWCNPICSPITAAARFHSCKCGNCPAKCDQCERDYGPP 6A90961E74341373 ifih1_human Interferon-induced helicase C domain-containing protein 1 Helicase with 2 CARD domains Interferon-induced with helicase C domain protein 1 Melanoma differentiation-associated protein 5 Murabutide down-regulated protein RNA helicase-DEAD box protein 116 RIG-I-like receptor 2 IFIH1 MDA5 RH116 Clinically amyopathic dermatomyositis autoantigen 140 kDa protein protein human Homo sapiens Human MSNGYSTDENFRYLISCFRARVKMYIQVEPVLDYLTFLPAEVKEQIQRTVATSGNMQAVELLLSTLEKGVWHLGWTREFVEALRRTGSPLAARYMNPELTDLPSPSFENAHDEYLQLLNLLQPTLVDKLLVRDVLDKCMEEELLTIEDRNRIAAAENNGNESGVRELLKRIVQKENWFSAFLNVLRQTGNNELVQELTGSDCSESNAEIENLSQVDGPQVEEQLLSTTVQPNLEKEVWGMENNSSESSFADSSVVSESDTSLAEGSVSCLDESLGHNSNMGSDSGTMGSDSDEENVAARASPEPELQLRPYQMEVAQPALEGKNIIICLPTGSGKTRVAVYIAKDHLDKKKKASEPGKVIVLVNKVLLVEQLFRKEFQPFLKKWYRVIGLSGDTQLKISFPEVVKSCDIIISTAQILENSLLNLENGEDAGVQLSDFSLIIIDECHHTNKEAVYNNIMRHYLMQKLKNNRLKKENKPVIPLPQILGLTASPGVGGATKQAKAEEHILKLCANLDAFTIKTVKENLDQLKNQIQEPCKKFAIADATREDPFKEKLLEIMTRIQTYCQMSPMSDFGTQPYEQWAIQMEKKAAKEGNRKERVCAEHLRKYNEALQINDTIRMIDAYTHLETFYNEEKDKKFAVIEDDSDEGGDDEYCDGDEDEDDLKKPLKLDETDRFLMTLFFENNKMLKRLAENPEYENEKLTKLRNTIMEQYTRTEESARGIIFTKTRQSAYALSQWITENEKFAEVGVKAHHLIGAGHSSEFKPMTQNEQKEVISKFRTGKINLLIATTVAEEGLDIKECNIVIRYGLVTNEIAMVQARGRARADESTYVLVAHSGSGVIEHETVNDFREKMMYKAIHCVQNMKPEEYAHKILELQMQSIMEKKMKTKRNIAKHYKNNPSLITFLCKNCSVLACSGEDIHVIEKMHHVNMTPEFKELYIVRENKALQKKCADYQINGEIICKCGQAWGTMMVHKGLDLPCLKIRNFVVVFKNNSTKKQYKKWVELPITFPNLDYSECCLFSDED DIP protein Q9BYX4 original sequence version: 130 DIP protein Q9BYX4 original sequence version: 120 DIP protein Q9BYX4 original sequence version: 123 DIP protein Q9BYX4 original sequence version: 66 DIP protein Q9BYX4 original sequence version: 82 DIP protein Q9BYX4-1 original sequence version: 136 DIP protein Q9BYX4-1 original sequence version: 107 DIP protein Q9BYX4 original sequence version: 122 789CFB4824B92DC9 v_mease Non-structural protein V P/V protein protein mease Measles virus (strain Edmonston) MeV Subacute sclerose panencephalitis virus MAEEQARHVKNGLECIRALKAEPIGSLAIEEAMAAWSEISDNPGQERATCREEKAGSSGLSKPCLSAIGSTEGGAPRIRGQGPGESDDDAETLGIPPRNLQASSTGLQCYYVYDHSGEAVKGIQDADSIMVQSGLDGDSTLSGGDNESENSDVDIGEPDTEGYAITDRGSAPISMGFRASDVETAEGGEIHELLRLQSRGNNFPKLGKTLNVPPPPDPGRASTSETPIKKGHRREISLIWNGDRVFIDRWCNPMCSKVTLGTIRARCTCGECPRVCEQCRTDTGVDTRIWYHNLPEIPE 8B545804E0CF25AD v_piv5 Non-structural protein V P/V protein protein sv5 Simian virus 5 (strain W3) PIV5 Simian virus 5 MDPTDLSFSPDEINKLIETGLNTVEYFTSQQVTGTSSLGKNTIPPGVTGLLTNAAEAKIQESTNHQKGSVGGGAKPKKPRPKIAIVPADDKTVPGKPIPNPLLGLDSTPSTQTVLDLSGKTLPSGSYKGVKLAKFGKENLMTRFIEEPRENPIATSSPIDFKRGRDTGGFHRREYSIGWVGDEVKVTEWCNPSCSPITAAARRFECTCHQCPVTCSECERDT DIP protein P11207 original sequence version: 53 DIP protein P11207 original sequence version: 80 13D2F1627D15AFA3 v_pi2h Non-structural protein V P/V protein protein pi2h human-parainfluenza-2-virus MAEEPTYTTEQVDELIHAGLGTVDFFLSRPIDAQSSLGKGSIPPGVTAVLTSAAEAKSKPVAAGPVKPRRKKVISNTTPYTIADNIPPEKLPINTPIPNPLLPLARPHGKMTDIDIVTGNITEGSYKGVELAKLGKQTLLTRFTSNEPVSSAGSAQDPNFKRGGANRERARGNHRREWSIAWVGDQVKVFEWCNPRCAPVTASARKFTCTCGSCPSICGECEGDH 3F96DA969F7F9CCC v_nipav Non-structural protein V P/V/C protein protein nipav Nipah virus MDKLELVNDGLNIIDFIQKNQKEIQKTYGRSSIQQPSIKDQTKAWEDFLQCTSGESEQVEGGMSKDDGDVERRNLEDLSSTSPTDGTIGKRVSNTRDWAEGSDDIQLDPVVTDVVYHDHGGECTGYGFTSSPERGWSDYTSGANNGNVCLVSDAKMLSYAPEIAVSKEDRETDLVHLENKLSTTGLNPTAVPFTLRNLSDPAKDSPVIAEHYYGLGVKEQNVGPQTSRNVNLDSIKLYTSDDEEADQLEFEDEFAGSSSEVIVGISPEDEEPSSVGGKPNESIGRTIEGQSIRDNLQAKDNKSTDVPGAGPKDSAVKEEPPQKRLPMLAEEFECSGSEDPIIRELLKENSLINCQQGKDAQPPYHWSIERSISPDKTEIVNGAVQTADRQRPGTPMPKSRGIPIKKGHRREISICWDGKRAWVEEWCNPACSRITPLPRRQECQCGECPTECFHCG 5E6735DC205B0EF9 dhx58-p_v-1 2 7 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association 1B, 2A and B, 3A ifih1-p_v 2 7 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association 1B-E, , 2A and B. 3A dhx58-p_v-3 2 9 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association 3A dhx58-p_v-5 2 3 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association 3A dhx58-p_v_c 2 4 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association 3A dhx58-p_v_c-2 2 3 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association 3A ifih1-p_v-2 2 9 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association 3A ifih1-p_v-4 2 6 unspecified role unspecified role bait bait 5 unspecified role unspecified role prey prey physical association physical association 3A ifih1-p_v_c 2 4 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association 3A ifih1-p_v_c-2 2 6 unspecified role unspecified role bait bait 10 unspecified role unspecified role prey prey physical association physical association 3A p_v-ifih1 2 7 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association 3B p_v-ifih1-1 2 5 unspecified role unspecified role bait bait 6 unspecified role unspecified role prey prey physical association physical association 3B p_v-dhx58 2 3 unspecified role unspecified role prey prey 5 unspecified role unspecified role bait bait physical association physical association 3B p_v-dhx58-1 2 7 unspecified role unspecified role bait bait 3 unspecified role unspecified role prey prey physical association physical association 3B ifih1-p_v-4 2 8 unspecified role unspecified role prey prey 6 unspecified role unspecified role bait bait physical association physical association 3A dhx58-p_v-4 2 8 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association 3A ifih1-p_v-3 1 6 unspecified role unspecified role bait bait 8 unspecified role unspecified role prey prey physical association physical association 4 and 6A dhx58-p_v-3 1 8 unspecified role unspecified role prey prey 3 unspecified role unspecified role bait bait physical association physical association 4 and 6B