IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ The high-affinity E. coli methionine ABC transporter: structure and allosteric regulation. The high-affinity E. coli methionine ABC transporter: structure and allosteric regulation. Science (0036-8075) 2008 imex curation Kadaba N.S., Kaiser J.T., Johnson E., Lee A., Rees D.C. Several crystal structures do not have a PDB id due to them not having a very high resolution. dcrees@caltech.edu 28-Oct_2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Kadaba N.S., Kaiser J.T., Johnson E., Lee A., Rees D.C. Several crystal structures do not have a PDB id due to them not having a very high resolution. dcrees@caltech.edu Accepted 2013-OCT-14 by MGT 28-Oct_2013: Contacted by IntAct-Help. Science (0036-8075) 2008 Only protein-protein interactions imex curation The high-affinity E. coli methionine ABC transporter: structure and allosteric regulation. The high-affinity E. coli methionine ABC transporter: structure and allosteric regulation. Science (0036-8075) 2008 imex curation Kadaba N.S., Kaiser J.T., Johnson E., Lee A., Rees D.C. Several crystal structures do not have a PDB id due to them not having a very high resolution. dcrees@caltech.edu 28-Oct_2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro atpase assay atpase assay predetermined predetermined participant predetermined Kadaba N.S., Kaiser J.T., Johnson E., Lee A., Rees D.C. Several crystal structures do not have a PDB id due to them not having a very high resolution. dcrees@caltech.edu Accepted 2013-OCT-14 by MGT 28-Oct_2013: Contacted by IntAct-Help. Science (0036-8075) 2008 Only protein-protein interactions imex curation metn_ecoli Methionine import ATP-binding protein MetN metN abc b0199 JW0195 protein protein ecoli Escherichia coli (strain K12) MIKLSNITKVFHQGTRTIQALNNVSLHVPAGQIYGVIGASGAGKSTLIRCVNLLERPTEGSVLVDGQELTTLSESELTKARRQIGMIFQHFNLLSSRTVFGNVALPLELDNTPKDEVKRRVTELLSLVGLGDKHDSYPSNLSGGQKQRVAIARALASNPKVLLCDEATSALDPATTRSILELLKDINRRLGLTILLITHEMDVVKRICDCVAVISNGELIEQDTVSEVFSHPKTPLAQKFIQSTLHLDIPEDYQERLQAEPFTDCVPMLRLEFTGQSVDAPLLSETARRFNVNNNIISAQMDYAGGVKFGIMLTEMHGTQQDTQAAIAWLQEHHVKVEVLGYV DIP protein P30750 original sequence version: 94 DIP protein P30750 original sequence version: null 2FC796C605216FB3 atp ATP Adenosine 5'-triphosphate Adenosine triphosphate ADENOSINE-5'-TRIPHOSPHATE Adephos Adetol Adynol Atipi ATP Atriphos Cardenosine Fosfobion Glucobasin H4atp Myotriphos Triadenyl Triphosphaden adenosine 5'-(tetrahydrogen triphosphate) small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) meti_ecoli D-methionine transport system permease protein MetI metI yaeE b0198 JW0194 protein protein ecoli Escherichia coli (strain K12) MSEPMMWLLVRGVWETLAMTFVSGFFGFVIGLPVGVLLYVTRPGQIIANAKLYRTVSAIVNIFRSIPFIILLVWMIPFTRVIVGTSIGLQAAIVPLTVGAAPFIARMVENALLEIPTGLIEASRAMGATPMQIVRKVLLPEALPGLVNAATITLITLVGYSAMGGAVGAGGLGQIGYQYGYIGYNATVMNTVLVLLVILVYLIQFAGDRIVRAVTRK DIP protein P31547 original sequence version: 80 EC1A22D772468EA8 meti-metn-1 1 3 unspecified role unspecified role neutral component neutral component n-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag n-term range n-terminal range n-term range n-term range n-terminal range n-term range ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 1, Table S1. 3.7 A R = 0.31; Rwork = 0.31; Rfree = 0.35 metn-1 1 3 unspecified role unspecified role neutral component neutral component c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range region sufficient to bind sufficient binding region sufficient to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) direct interaction direct interaction Fig. 3b, Table S2 2.1 A R = 0.19; Rwork = 0.19; Rfree = 0.24 meti-metn-4 2 4 enzyme target enzyme target substrate substrate neutral component neutral component chemical synthesis Chemical synthesis (Chemical synthesis) 3 enzyme enzyme neutral component neutral component region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined P30750:p.Glu166Gln mutation disrupting mutation disrupting interaction mutation disrupting certain certain sequence position certain certain certain sequence position certain E Q Wed May 02 19:20:27 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu166gln' Tue Jun 30 19:56:42 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Glu166Gln' c2_region required to bind necessary binding region required to bind certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 ancillary ancillary neutral component neutral component ecoli Escherichia coli (strain K12) atpase reaction atpase reaction Fig.s 4, S3 L-methionine & L-selenomethionine (concentration-dependent) The MetN-C2 construct (aa 247-343 + 6xHis) showed a L-methionine-concentration-independent reduction of ATPase activity of ~50%.