matrixdb Institut de Biologie et de Chimie des Prot\u00c3\u00a9ines, 7, passage du Vercors 69 367 Lyon cedex 07 FRANCE matrixdb@ibcp.fr http://matrixdb.ibcp.fr Direct binding of integrin alphavbeta3 to FGF1 plays a role in FGF1 signaling. Direct binding of integrin alphavbeta3 to FGF1 plays a role in FGF1 signaling. J. Biol. Chem. (0021-9258) 2008 imex curation Mori S., Wu C.Y., Yamaji S., Saegusa J., Shi B., Ma Z., Kuwabara Y., Lam K.S., Isseroff R.R., Takada Y.K., Takada Y. ytakada@ucdavis.edu 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro spr surface plasmon resonance Optical biosensor BIAcore(r) BIAcore(r) Optical biosensor spr predetermined predetermined participant predetermined Mori S., Wu C.Y., Yamaji S., Saegusa J., Shi B., Ma Z., Kuwabara Y., Lam K.S., Isseroff R.R., Takada Y.K., Takada Y. ytakada@ucdavis.edu Accepted 2013-FEB-14 by ORCHARD 01-March-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2008 Only protein-protein interactions imex curation Direct binding of integrin alphavbeta3 to FGF1 plays a role in FGF1 signaling. Direct binding of integrin alphavbeta3 to FGF1 plays a role in FGF1 signaling. J. Biol. Chem. (0021-9258) 2008 imex curation Mori S., Wu C.Y., Yamaji S., Saegusa J., Shi B., Ma Z., Kuwabara Y., Lam K.S., Isseroff R.R., Takada Y.K., Takada Y. ytakada@ucdavis.edu 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro elisa enzyme linked immunosorbent assay ELISA ELISA elisa antibody detection identification by antibody antibody detection Mori S., Wu C.Y., Yamaji S., Saegusa J., Shi B., Ma Z., Kuwabara Y., Lam K.S., Isseroff R.R., Takada Y.K., Takada Y. ytakada@ucdavis.edu Accepted 2013-FEB-14 by ORCHARD 01-March-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2008 Only protein-protein interactions imex curation Mock publication and experiment for abstract interactions that are not interaction evidences. Mock publication and experiment for abstract interactions that are not interaction evidences. unspecified method Direct binding of integrin alphavbeta3 to FGF1 plays a role in FGF1 signaling. Direct binding of integrin alphavbeta3 to FGF1 plays a role in FGF1 signaling. J. Biol. Chem. (0021-9258) 2008 imex curation Mori S., Wu C.Y., Yamaji S., Saegusa J., Shi B., Ma Z., Kuwabara Y., Lam K.S., Isseroff R.R., Takada Y.K., Takada Y. ytakada@ucdavis.edu 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro affinity chrom affinity chromatography technology Affinity purification Affinity purification affinity chrom weight by staining molecular weight estimation by staining weight by staining Mori S., Wu C.Y., Yamaji S., Saegusa J., Shi B., Ma Z., Kuwabara Y., Lam K.S., Isseroff R.R., Takada Y.K., Takada Y. ytakada@ucdavis.edu Accepted 2013-FEB-14 by ORCHARD 01-March-2013: Contacted by IntAct-Help. J. Biol. Chem. (0021-9258) 2008 Only protein-protein interactions imex curation fgf1_human Fibroblast growth factor 1 Acidic fibroblast growth factor FGF1 FGFA Heparin-binding growth factor 1 Endothelial cell growth factor protein protein human Homo sapiens Human MAEGEITTFTALTEKFNLPPGNYKKPKLLYCSNGGHFLRILPDGTVDGTRDRSDQHIQLQLSAESVGEVYIKSTETGQYLAMDTDGLLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVGLKKNGSCKRGPRTHYGQKAILFLPLPVSSD F586E8BFB09F1580 itb3_human Integrin beta-3 Platelet membrane glycoprotein IIIa ITGB3 GP3A protein protein human Homo sapiens Human MRARPRPRPLWATVLALGALAGVGVGGPNICTTRGVSSCQQCLAVSPMCAWCSDEALPLGSPRCDLKENLLKDNCAPESIEFPVSEARVLEDRPLSDKGSGDSSQVTQVSPQRIALRLRPDDSKNFSIQVRQVEDYPVDIYYLMDLSYSMKDDLWSIQNLGTKLATQMRKLTSNLRIGFGAFVDKPVSPYMYISPPEALENPCYDMKTTCLPMFGYKHVLTLTDQVTRFNEEVKKQSVSRNRDAPEGGFDAIMQATVCDEKIGWRNDASHLLVFTTDAKTHIALDGRLAGIVQPNDGQCHVGSDNHYSASTTMDYPSLGLMTEKLSQKNINLIFAVTENVVNLYQNYSELIPGTTVGVLSMDSSNVLQLIVDAYGKIRSKVELEVRDLPEELSLSFNATCLNNEVIPGLKSCMGLKIGDTVSFSIEAKVRGCPQEKEKSFTIKPVGFKDSLIVQVTFDCDCACQAQAEPNSHRCNNGNGTFECGVCRCGPGWLGSQCECSEEDYRPSQQDECSPREGQPVCSQRGECLCGQCVCHSSDFGKITGKYCECDDFSCVRYKGEMCSGHGQCSCGDCLCDSDWTGYYCNCTTRTDTCMSSNGLLCSGRGKCECGSCVCIQPGSYGDTCEKCPTCPDACTFKKECVECKKFDRGALHDENTCNRYCRDEIESVKELKDTGKDAVNCTYKNEDDCVVRFQYYEDSSGKSILYVVEEPECPKGPDILVVLLSVMGAILLIGLAALLIWKLLITIHDRKEFAKFEEERARAKWDTANNPLYKEATSTFTNITYRGT DIP protein P05106 original sequence version: 202 DIP protein P05106-1 original sequence version: 158 DIP protein P05106-1 original sequence version: 164 DIP protein P05106 original sequence version: 200 DIP protein P05106-1 original sequence version: 213 DIP protein P05106-1 original sequence version: 175 DIP protein P05106-1 original sequence version: 192 DIP protein P05106-1 original sequence version: 171 F246623608E05F9E heparin Heparin small molecule small molecule chemical synthesis Chemical synthesis (Chemical synthesis) itav_human Integrin alpha-V ITGAV VNRA Vitronectin receptor subunit alpha MSK8 Vitronectin receptor VTNR protein protein human Homo sapiens Human MAFPPRRRLRLGPRGLPLLLSGLLLPLCRAFNLDVDSPAEYSGPEGSYFGFAVDFFVPSASSRMFLLVGAPKANTTQPGIVEGGQVLKCDWSSTRRCQPIEFDATGNRDYAKDDPLEFKSHQWFGASVRSKQDKILACAPLYHWRTEMKQEREPVGTCFLQDGTKTVEYAPCRSQDIDADGQGFCQGGFSIDFTKADRVLLGGPGSFYWQGQLISDQVAEIVSKYDPNVYSIKYNNQLATRTAQAIFDDSYLGYSVAVGDFNGDGIDDFVSGVPRAARTLGMVYIYDGKNMSSLYNFTGEQMAAYFGFSVAATDINGDDYADVFIGAPLFMDRGSDGKLQEVGQVSVSLQRASGDFQTTKLNGFEVFARFGSAIAPLGDLDQDGFNDIAIAAPYGGEDKKGIVYIFNGRSTGLNAVPSQILEGQWAARSMPPSFGYSMKGATDIDKNGYPDLIVGAFGVDRAILYRARPVITVNAGLEVYPSILNQDNKTCSLPGTALKVSCFNVRFCLKADGKGVLPRKLNFQVELLLDKLKQKGAIRRALFLYSRSPSHSKNMTISRGGLMQCEELIAYLRDESEFRDKLTPITIFMEYRLDYRTAADTTGLQPILNQFTPANISRQAHILLDCGEDNVCKPKLEVSVDSDQKKIYIGDDNPLTLIVKAQNQGEGAYEAELIVSIPLQADFIGVVRNNEALARLSCAFKTENQTRQVVCDLGNPMKAGTQLLAGLRFSVHQQSEMDTSVKFDLQIQSSNLFDKVSPVVSHKVDLAVLAAVEIRGVSSPDHVFLPIPNWEHKENPETEEDVGPVVQHIYELRNNGPSSFSKAMLHLQWPYKYNNNTLLYILHYDIDGPMNCTSDMEINPLRIKISSLQTTEKNDTVAGQGERDHLITKRDLALSEGDIHTLGCGVAQCLKIVCQVGRLDRGKSAILYVKSLLWTETFMNKENQNHSYSLKSSASFNVIEFPYKNLPIEDITNSTLVTTNVTWGIQPAPMPVPVWVIILAVLAGLLLLAVLVFVMYRMGFFKRVRPPQEEQEREQLQPHENGEGNSET DIP protein P06756 original sequence version: 136 DIP protein P06756 original sequence version: 145 DIP protein P06756 original sequence version: 174 DIP protein P06756-1 original sequence version: 182 DIP protein P06756 original sequence version: 149 364EE25C5303A2D7 fgfr1_human Fibroblast growth factor receptor 1 FGFR1 FGFBR FLG FLT2 Fms-like tyrosine kinase 2 Proto-oncogene c-Fgr BFGFR CEK HBGFR Basic fibroblast growth factor receptor 1 N-sam protein protein human Homo sapiens Human MWSWKCLLFWAVLVTATLCTARPSPTLPEQAQPWGAPVEVESFLVHPGDLLQLRCRLRDDVQSINWLRDGVQLAESNRTRITGEEVEVQDSVPADSGLYACVTSSPSGSDTTYFSVNVSDALPSSEDDDDDDDSSSEEKETDNTKPNRMPVAPYWTSPEKMEKKLHAVPAAKTVKFKCPSSGTPNPTLRWLKNGKEFKPDHRIGGYKVRYATWSIIMDSVVPSDKGNYTCIVENEYGSINHTYQLDVVERSPHRPILQAGLPANKTVALGSNVEFMCKVYSDPQPHIQWLKHIEVNGSKIGPDNLPYVQILKTAGVNTTDKEMEVLHLRNVSFEDAGEYTCLAGNSIGLSHHSAWLTVLEALEERPAVMTSPLYLEIIIYCTGAFLISCMVGSVIVYKMKSGTKKSDFHSQMAVHKLAKSIPLRRQVTVSADSSASMNSGVLLVRPSRLSSSGTPMLAGVSEYELPEDPRWELPRDRLVLGKPLGEGCFGQVVLAEAIGLDKDKPNRVTKVAVKMLKSDATEKDLSDLISEMEMMKMIGKHKNIINLLGACTQDGPLYVIVEYASKGNLREYLQARRPPGLEYCYNPSHNPEEQLSSKDLVSCAYQVARGMEYLASKKCIHRDLAARNVLVTEDNVMKIADFGLARDIHHIDYYKKTTNGRLPVKWMAPEALFDRIYTHQSDVWSFGVLLWEIFTLGGSPYPGVPVEELFKLLKEGHRMDKPSNCTNELYMMMRDCWHAVPSQRPTFKQLVEDLDRIVALTSNQEYLDLSMPLDQYSPSFPDTRSSTCSSGEDSVFSHEPLPEEPCLPRHPAQLANGGLKRR DIP protein P11362 original sequence version: 154 DIP protein P11362 original sequence version: 160 DIP protein P11362 original sequence version: 180 DIP protein P11362 original sequence version: 170 93A01B5D78C3E72C fgf1-intg-1 2 12 unspecified role unspecified role prey prey c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 5 unspecified role unspecified role bait bait P05230:p.[Lys127Glu;Lys128Glu] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain K E certain certain sequence position certain certain certain sequence position certain K E Wed May 02 16:24:30 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys127glu,lys128glu' Tue Jun 30 20:12:47 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys127Glu;Lys128Glu]' P05230:p.[Lys133Glu;Arg134Glu] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain K E certain certain sequence position certain certain certain sequence position certain R E Wed May 02 16:54:16 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys133glu,arg134glu' Tue Jun 30 18:47:15 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys133Glu;Arg134Glu]' P05230:p.[Lys127Glu;Lys128Glu;Lys133Glu;Arg134Glu] mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain K E certain certain sequence position certain certain certain sequence position certain K E certain certain sequence position certain certain certain sequence position certain K E certain certain sequence position certain certain certain sequence position certain R E Wed May 02 16:14:29 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys127glu,lys128glu,lys133glu,arg134glu' Tue Jun 30 15:59:45 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys127Glu;Lys128Glu;Lys133Glu;Arg134Glu]' P05230:p.Arg50Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain R E Wed May 02 14:40:02 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg50glu' Tue Jun 30 18:58:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg50Glu' ecoli Escherichia coli (strain K12) direct interaction direct interaction 1a, 3a, 3b The binding to FGF1 is cation-dependent and requires an intact three-dimensional structure of FGF1 intg-intg 1 5 unspecified role unspecified role prey prey P05230:p.Arg50Glu mutation decreasing mutation decreasing interaction hotspot hotspot mutation decreasing predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain R E Wed May 02 16:22:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg50glu' Tue Jun 30 17:54:33 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg50Glu' ecoli Escherichia coli (strain K12) 12 unspecified role unspecified role bait bait c-terminal his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag c-term range C-terminal range c-term range c-term range C-terminal range c-term range crigr-cho_k1 Cricetulus griseus ovary K1 cho_k1 Chinese Hamster Ovary K1 direct interaction direct interaction 3c, 3d The R50E mutant showed much lower Rmax value and enhanced off-rate to alpha beta 3, whereas it has a KD value to alpha beta 3 (1.31 uM) similar to WT FGF1 fgfr1-fgf1 1 9 unspecified role unspecified role bait bait binding region sufficient to bind sufficient binding region sufficient to bind predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 5 unspecified role unspecified role prey prey P05230:p.[Glu102Ala;Tyr109Ala;Asn110Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain E A certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain N A Wed May 02 16:17:38 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu102ala,tyr109ala,asn110ala' Tue Jun 30 17:46:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu102Ala;Tyr109Ala;Asn110Ala]' P05230:p.[Lys127Glu;Lys128Glu;Lys133Glu;Arg134Glu] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain K E certain certain sequence position certain certain certain sequence position certain K E certain certain sequence position certain certain certain sequence position certain K E certain certain sequence position certain certain certain sequence position certain R E Wed May 02 15:48:11 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys127glu,lys128glu,lys133glu,arg134glu' Tue Jun 30 19:41:38 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys127Glu;Lys128Glu;Lys133Glu;Arg134Glu]' ecoli Escherichia coli (strain K12) direct interaction direct interaction 4a-c heparin-fgf1 1 5 unspecified role unspecified role prey prey P05230:p.Arg50Lys mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain R K Wed May 02 19:59:44 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'arg50lys' Tue Jun 30 18:27:25 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Arg50Lys' P05230:p.[Glu102Ala;Tyr109Ala;Asn110Ala] mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain E A certain certain sequence position certain certain certain sequence position certain Y A certain certain sequence position certain certain certain sequence position certain N A Wed May 02 15:29:13 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'glu102ala,tyr109ala,asn110ala' Tue Jun 30 19:47:30 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Glu102Ala;Tyr109Ala;Asn110Ala]' ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait pig-gastric mucosa Sus scrofa gastric mucosa gastric_mucosa gastric mucosa direct interaction direct interaction 4e, 4f heparin-fgf1-1 4 5 unspecified role unspecified role prey prey P05230:p.[Lys127Glu;Lys128Glu;Lys133Glu;Arg134Glu] mutation disrupting strength mutation disrupting interaction strength mutation disrupting strength predetermined featur predetermined feature predetermined featur certain certain sequence position certain certain certain sequence position certain K E certain certain sequence position certain certain certain sequence position certain K E certain certain sequence position certain certain certain sequence position certain K E certain certain sequence position certain certain certain sequence position certain R E Wed May 02 18:02:56 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'lys127glu,lys128glu,lys133glu,arg134glu' Tue Jun 30 17:36:39 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.[Lys127Glu;Lys128Glu;Lys133Glu;Arg134Glu]' ecoli Escherichia coli (strain K12) 7 unspecified role unspecified role bait bait pig-gastric mucosa Sus scrofa gastric mucosa gastric_mucosa gastric mucosa direct interaction direct interaction 4d intg_av_b3_human Integrin alpha-v/beta-3 alphav-beta3 integrin complex alpha-v/beta-3 integrin complex Integrin alphav-beta3 complex ITGAV:ITGB3 6 unspecified role unspecified role 8 unspecified role unspecified role direct interaction direct interaction human Homo sapiens Human stable complex stable complex physical interaction evidence used in manual assertion physical interaction evidence used in manual assertion IPI inferred from physical interaction Cell adhesion, bi-directional signaling receptor which functions as a link between the extra-cellular matrix and the cytoskeleton. Receptor for vitronectin, cytotactin, fibronectin, fibrinogen, laminin, matrix metalloproteinase-2, osteopontin, osteomodulin, prothrombin, thrombospondin and von Willebrand factor which its binds via the sequence R-G-D in the ligand. Heterodimer of an alpha and a beta subunit. The alpha subunit is composed of an heavy and a light chain linked by a disulfide bond. The ligand-binding site forms in a region at the intersection of the integrin alpha-chain beta-propeller and the betaI domain, with the alpha chain being central in determining ligand specificity. Heterodimer created with intact-jami-dbscripts