HPIDb Host Pathogen Interaction database http://www.agbase.msstate.edu/hpi/main.html IkappaB kinase beta-induced phosphorylation of CARMA1 contributes to CARMA1 Bcl10 MALT1 complex formation in B cells. IkappaB kinase beta-induced phosphorylation of CARMA1 contributes to CARMA1 Bcl10 MALT1 complex formation in B cells. J. Exp. Med. (0022-1007) 2007 imex curation Shinohara H., Maeda S., Watarai H., Kurosaki T. kurosaki@rcai.riken.jp 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation in vitro In vitro protein kinase assay protein kinase assay western blot western blot Immuno blot Immuno blot Shinohara H., Maeda S., Watarai H., Kurosaki T. kurosaki@rcai.riken.jp Accepted 2013-MAY-31 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. J. Exp. Med. (0022-1007) 2007 imex curation IkappaB kinase beta-induced phosphorylation of CARMA1 contributes to CARMA1 Bcl10 MALT1 complex formation in B cells. IkappaB kinase beta-induced phosphorylation of CARMA1 contributes to CARMA1 Bcl10 MALT1 complex formation in B cells. J. Exp. Med. (0022-1007) 2007 imex curation Shinohara H., Maeda S., Watarai H., Kurosaki T. kurosaki@rcai.riken.jp 17-Jun-2013: Contacted by IntAct-Help. Only protein-protein interactions imex curation chick-dt40 Gallus gallus bursa lymphoma dt40 chicken bursa lymphoma http://www.lgcpromochem-atcc.com/common/catalog/numSearch/numResults.cfm chicken bursa lymphoma anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Shinohara H., Maeda S., Watarai H., Kurosaki T. kurosaki@rcai.riken.jp Accepted 2013-MAY-31 by ORCHARD 17-Jun-2013: Contacted by IntAct-Help. J. Exp. Med. (0022-1007) 2007 imex curation e1c736_chick MALT1 protein protein chick Gallus gallus (Chicken) Chicken AVSRFLQLLEKGVYAIFYYAGHGYEHLGRNYMVPVDAPQLYAPENCISVQRILQKMQQQQTALNLILLDTCRKWYNPECALSQVQPLEPWGNTVYGYATSEDAEAYELQNGEFSSGIFMKYLKKHILQEKKVTHMLEDVLEDLGRDPVVTGKQVMEIKHTLKEARSLTDPICPTGAATERWGCSREPPSETVTFPCGVQAELRFHRLFSNVLSVCAKLQDLPAHLTDAQL The sequence has been withdrawn from uniprot. DA9D2A68CFCCA780 a0a1d5pfv1_chick I-kappa-B kinase IKBKB protein protein chick Gallus gallus (Chicken) Chicken MSRPPALQAQTCGPWEMKERLGTGGFGNVIRWHNKETGEQVAIKQCRQELSPRNRDRWALEIQIMKRLNHPNVVAARDVPEGMQKLAPNDLPLLAMEYCQGGDLRKYLNQLENCCGLREEAILILLSDIASALRYLHENRIIHRDLKPENIVLQQGEQRLIHKIIDLGYAKELDQGSLCTSFVGTLQYLAPELLEQQKYTVTVDYWSFGTLAFECITGFRPFLPNWQPVQWHTKVRQKSELDIVVSEDLSGEVKFSSSLPCPNNLNSVLAGKLEKWLQLMLMWHPRQRGTDPVYGPNGCFKALDDILNLKLLHVLNMVTGTVHTYPVTEEETLQTVKARIQSDTGIPEQDQELLQEAGLALFTQKHTADSKVNDAVVADTDLLFLFDNQKVAYEAQVALRPHPESVDYILQDPKKNLHFFQLRKVWGQIWHTIRMLKEDCNRLQQGQRAAMMNLLRYNSTLSKMKNSMASLSQQLKAKLDFFKTSIQIDLEKYKEQIEFGITSEKLLFAWKEMEQAVELCGREDDVDQLVKRMMALQTDIVDLQRSPLGRKQGGTLEDLEEQARELYRRLREKPRDQRTSGDSQEIVRLLLQAIQTFEKKVRVIYAQLSKTVVCKQKALELFPRVEKVMNLMNEDEETVVRLQEKRQKELWNLLKIACSKVRGPVSGSPESMNMSRLNSPGQLLLQVPSGTYALSESVRKSEELLLESQKLCSQLENVMHDTMKDQEQSFMALDWSWLQLHVEERSSPEQTHM 2ED6D3DE7B89EE69 f1nkg4_chick BCL10 protein protein chick Gallus gallus (Chicken) Chicken SSAVGRPLTEDEMADVKKEALERMRPYLCDKIIAERHFDYLRSKKILTREDTEEISSRSSSRKKTGKLLDYLAENPKGLDALVESIRRERTQNFLLQKITDIVLKVKNEKLEALKGLSCSTCMTSLYGGTNNLSRSYSDESNFFDKTKDKESTQMHNPEEDYSTAAFMSAVSLHSMNLPVAEMGNAESAVFSVTLPGPGDPGAPPLPPELQSEQQEPCTSSSDNCFLPLRSRSLHPQ The sequence has been withdrawn from uniprot. 6E9796811BCDD0FE q5f3w0_chick CARD11 RCJMB04_5k17 protein protein chick Gallus gallus (Chicken) Chicken MSTQGGEPEMDDCLETLKDEEEALWENVECNRHMLSRYINPAKLTPYLRQCKVIDEQDEDEVLNSLMLPSKINRAGRLLDILHTKGQRGYVVFLESLEFYYPELYKLVTGKEPTRRFSTIVVEEGHEGLTHFLMNEIIKLQQQVKTKDMQRCDLLAKSRQLEDERKQLKLNKIELMTFQERYNKMKEERNNYNDELVKVKDENYNLAMRYAQLSDEKSMAVMRSRDLQLEIDQLKHRLNKVEEECKLERNQSLKLKNDIENRPKKEQMLELERENEMMKTKIQELQSIIQADKRSLPDSDKAILDILEHDRKEALEDRHELVNKIFNLQEEIRHVEDLRDKYLEEKEDLELKCSTLGKDCEMYKHRMNTVMIQLEEVEKERDQAFRSRDEAQTQYSHCLIEKDKYRKQIRELEERNDELRIEMVRKEACIVNLECKLRRLSKDNNCHDQSLPRNLPITIISQTFGNSSPKANGQEADDSSTSEDSPEDNKFFLPDQARLKRRVNLKGIQINPRAKSPVSMNRTSEFQAVRAQDEDGANASNGRTDTSSSNSVSISNSISSCEVSKIQTLRNRNDSIMSTTPEPPGNDSIVRRCKEDAPPCSMVEEDNDSFGFDALELDDDSHDRHSHGAPSVHSSSSSHQSEGLDAYDLEHVNSIFRKFSLERPFRPSVTSVGRIRSSCHTIQRITLNGDTLNSEITLIGGNDKGSFVSSVVSGSLAEKAGLREGHQLLLLEGCIKGENQSVPLDTCTKEEVHWTIQRCHGPVTLQYKSNHEGYRKLLSELEDGLIASGDSFHIRLNLNISSQLDCCSLSVKCDEIVHILDTMYQGTCGGCDWLCARVDPFTDKDLERGTIPSYSRAQQLLLVKLQRLMHRGSKDETESSHNTLRALRNTLQPEEPAPQSDPKTSPRLSRASFLLGQILQFVSRSENKYKRMNSNERVRIVTGWPSGLARTASEAKKPFPDKLEDLDSKSEINKRLIPYSLVRPIHCERRRPVLFTPTMLAKSLVQKLLNSGGALEFDICKPDIVTKEEFLRRQRMETIIFSREKNLSTYECIVPANIEAVSAKNKHCLLEAGISCTKDLIKAKIYPIVLFIRVSEKNIKRFRKLLPKPEAEDEFLRMCRLKEKELEELPCLYASVEADAWSSIEDLIRTIKDKIGEEQRKTIWIDEDQL A964C1E9DD169D27 f1n9k8_chick PRKCB protein protein chick Gallus gallus (Chicken) Chicken VCCFVVHKRCHEFVTFSCPGADKGPASDDPRSKHKFKIHTYSSPTFCDHCGSLLYGLIHQGMKCDTCMMNVHKRCVMNVPSLCGTDHTERRGRIYIRADIEKDVLTVVVRDAKNLVPMDPNGLSDPYVKLKLIPDPKNESKQKTKTIKCSLNPEWNETFRFQLKEADKDRRLSVEIWDWDLTSRNDFMGSLSFGISELQKSGVDGWFKLLSQEEGEYFNVPVPPEGEEGNEELRQKFERAKIGTGSKAADEKTTNAISKFDNNGSRDRMKLSDFNFLMVLGKGSFGKVMLAERKGTDELYAVKILKKDVVIQDDDVECTMVEKRVLALSGKPPFLTQLHSCFQTMDRLYFVMEYVNGGDLMYQIQQVGRFKEPHAVFYAAEIAIGLFFLQSKGIIYRDLKLDNVMLDSEGHIKIADFGMCKENIWDGVTTKTFCGTPDYIAPEIIAYQPYGKSVDWWAFGVLLYEMLAGQAPFEGEDEDELFQSIMEHNVAYPKSMSKEAVAICKGLMTKHPAKRLGCGPEGERDIKEHAFFRYIDWDKLERNSVAPPYIPSLCCGRNAENFDRFFTRHPPVLTPPDQEVIRNIDQSEFEGFSFFNSEFFKPEAKSST The sequence has been withdrawn from uniprot. F2EEE06A27079F74 pkcb-carma1-1 1 PKCB 7 enzyme enzyme unspecified role unspecified role chick-dt40 Gallus gallus bursa lymphoma dt40 chicken bursa lymphoma http://www.lgcpromochem-atcc.com/common/catalog/numSearch/numResults.cfm chicken bursa lymphoma CARMA1 6 enzyme target enzyme target substrate substrate unspecified role unspecified role carma1_flagtag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chick-dt40 Gallus gallus bursa lymphoma dt40 chicken bursa lymphoma http://www.lgcpromochem-atcc.com/common/catalog/numSearch/numResults.cfm chicken bursa lymphoma phosphorylation phosphorylation reaction phosphorylation 2B and C PKCB phosphorylate Ser668 on CARMA1. PKCB deficient DT40 cells abolished Ser668 phosphorylation. ikkb-carma1_1-1 1 4 enzyme enzyme experimental role experimental role chick-dt40 Gallus gallus bursa lymphoma dt40 chicken bursa lymphoma http://www.lgcpromochem-atcc.com/common/catalog/numSearch/numResults.cfm chicken bursa lymphoma CARMA1 6 enzyme target enzyme target substrate substrate experimental role experimental role carma1_flagtag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined chick-dt40 Gallus gallus bursa lymphoma dt40 chicken bursa lymphoma http://www.lgcpromochem-atcc.com/common/catalog/numSearch/numResults.cfm chicken bursa lymphoma phosphorylation phosphorylation reaction phosphorylation 4F IKKB phosphorylate Ser578 on CARMA1. carma1-bcl10-malt1-1 2 CARMA1 6 unspecified role unspecified role bait bait nucl transfection nucleic acid transfection nucl transfection Q5F3W0:p.Thr119Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain T A Wed May 02 14:33:22 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'thr119ala' Tue Jun 30 17:16:58 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Thr119Ala' Q5F3W0:p.Ser578Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A Wed May 02 14:24:48 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser578ala' Tue Jun 30 19:17:46 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser578Ala' Q5F3W0:p.Ser668Ala mutation decreasing strength mutation decreasing interaction strength mutation decreasing strength certain certain sequence position certain certain certain sequence position certain S A Wed May 02 17:09:15 BST 2018 This feature has been corrected as a result of our quality control procedures. The original label was 'ser668ala' Tue Jun 30 15:41:32 BST 2020 This feature has been corrected as a result of our quality control procedures. The original label was 'p.Ser668Ala' carma1_flagtag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged western blot western blot Immuno blot Immuno blot undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined Bcl10 5 unspecified role unspecified role prey prey MALT1 3 unspecified role unspecified role prey prey association association 2D, 4E and 5B S668A or Sre578 CARMA1 mutant failed to make inducible association with Bcl10 and MALT1. In IKKB deficient (Fig 5C) and kinase-inactive IKKB (Fig 4B), the initial CARMA-Bcl10-MALT1 association was significantly reduced. You also need to add an anti-bait coip expt for the interaction with the non-tagged, endogenous protein in 5C - treat this as one interaction , not 2.