mbinfo MBInfo http://www.mechanobio.info/ Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation. Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation. J. Cell Biol. (0021-9525) 2007 imex curation Brandt D.T., Marion S., Griffiths G., Watanabe T., Kaibuchi K., Grosse R. The source of Dia1 and IQGAP1 (both enogenous) inferred from the cells (HeLa) used. Robert.Grosse@pharma.uni-heidelberg.de 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down anti tag western anti tag western blot anti tag western Brandt D.T., Marion S., Griffiths G., Watanabe T., Kaibuchi K., Grosse R. Interaction detection: GST pull down. The source of Dia1 and IQGAP1 (both enogenous) inferred from the cells (HeLa) used. Robert.Grosse@pharma.uni-heidelberg.de Accepted 2013-FEB-19 by ORCHARD 01-March-2013: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 2007 Only protein-protein interactions imex curation Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation. Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation. J. Cell Biol. (0021-9525) 2007 imex curation Brandt D.T., Marion S., Griffiths G., Watanabe T., Kaibuchi K., Grosse R. The source of Dia1 and IQGAP1 (both enogenous) inferred from the cells (HeLa) used. Robert.Grosse@pharma.uni-heidelberg.de 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Brandt D.T., Marion S., Griffiths G., Watanabe T., Kaibuchi K., Grosse R. The source of Dia1 and IQGAP1 (both enogenous) inferred from the cells (HeLa) used. Robert.Grosse@pharma.uni-heidelberg.de Accepted 2013-FEB-19 by ORCHARD 01-March-2013: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 2007 Only protein-protein interactions imex curation Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation. Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation. J. Cell Biol. (0021-9525) 2007 imex curation Brandt D.T., Marion S., Griffiths G., Watanabe T., Kaibuchi K., Grosse R. The source of Dia1 and IQGAP1 (both enogenous) inferred from the cells (HeLa) used. Robert.Grosse@pharma.uni-heidelberg.de 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 anti bait coip anti bait coimmunoprecipitation anti bait coip western blot western blot Immuno blot Immuno blot Brandt D.T., Marion S., Griffiths G., Watanabe T., Kaibuchi K., Grosse R. The source of Dia1 and IQGAP1 (both enogenous) inferred from the cells (HeLa) used. Robert.Grosse@pharma.uni-heidelberg.de Accepted 2013-FEB-19 by ORCHARD 01-March-2013: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 2007 Only protein-protein interactions imex curation Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation. Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation. J. Cell Biol. (0021-9525) 2007 imex curation Brandt D.T., Marion S., Griffiths G., Watanabe T., Kaibuchi K., Grosse R. The source of Dia1 and IQGAP1 (both enogenous) inferred from the cells (HeLa) used. Robert.Grosse@pharma.uni-heidelberg.de 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Brandt D.T., Marion S., Griffiths G., Watanabe T., Kaibuchi K., Grosse R. The source of Dia1 and IQGAP1 (both enogenous) inferred from the cells (HeLa) used. Robert.Grosse@pharma.uni-heidelberg.de Accepted 2013-FEB-19 by ORCHARD 01-March-2013: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 2007 Only protein-protein interactions imex curation Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation. Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation. J. Cell Biol. (0021-9525) 2007 imex curation Brandt D.T., Marion S., Griffiths G., Watanabe T., Kaibuchi K., Grosse R. The source of Dia1 and IQGAP1 (both enogenous) inferred from the cells (HeLa) used. Robert.Grosse@pharma.uni-heidelberg.de 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down western blot western blot Immuno blot Immuno blot Brandt D.T., Marion S., Griffiths G., Watanabe T., Kaibuchi K., Grosse R. Interaction detection: GST pull down. The source of Dia1 and IQGAP1 (both enogenous) inferred from the cells (HeLa) used. Robert.Grosse@pharma.uni-heidelberg.de Accepted 2013-FEB-19 by ORCHARD 01-March-2013: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 2007 Only protein-protein interactions imex curation Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation. Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation. J. Cell Biol. (0021-9525) 2007 imex curation Brandt D.T., Marion S., Griffiths G., Watanabe T., Kaibuchi K., Grosse R. The source of Dia1 and IQGAP1 (both enogenous) inferred from the cells (HeLa) used. Robert.Grosse@pharma.uni-heidelberg.de 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down weight by comassie molecular weight estimation by coomasie staining weight by comassie Brandt D.T., Marion S., Griffiths G., Watanabe T., Kaibuchi K., Grosse R. Interaction detection: GST pull down assay. The source of Dia1 and IQGAP1 (both enogenous) inferred from the cells (HeLa) used. Robert.Grosse@pharma.uni-heidelberg.de Accepted 2013-FEB-19 by ORCHARD 01-March-2013: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 2007 Only protein-protein interactions imex curation Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation. Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation. J. Cell Biol. (0021-9525) 2007 imex curation Brandt D.T., Marion S., Griffiths G., Watanabe T., Kaibuchi K., Grosse R. The source of Dia1 and IQGAP1 (both enogenous) inferred from the cells (HeLa) used. Robert.Grosse@pharma.uni-heidelberg.de 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro pull down pull down ms participant Identification by mass spectrometry ms participant Brandt D.T., Marion S., Griffiths G., Watanabe T., Kaibuchi K., Grosse R. Interaction detection: GST pull down. Participant detection: Bound proteins were separated on SDS-PAGE and visualized by coomassie staining. The bands of interest were excised and identified by mass sprectrometry. The source of Dia1 and IQGAP1 (both enogenous) inferred from the cells (HeLa) used. Robert.Grosse@pharma.uni-heidelberg.de Accepted 2013-FEB-19 by ORCHARD 01-March-2013: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 2007 Only protein-protein interactions imex curation Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation. Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation. J. Cell Biol. (0021-9525) 2007 imex curation Brandt D.T., Marion S., Griffiths G., Watanabe T., Kaibuchi K., Grosse R. The source of Dia1 and IQGAP1 (both enogenous) inferred from the cells (HeLa) used. Robert.Grosse@pharma.uni-heidelberg.de 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-raw264_7 Mus musculus monocyte macrophage cell line RAW264 raw264_7 Mouse monocyte macrophage cellline RAW264.7 fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Brandt D.T., Marion S., Griffiths G., Watanabe T., Kaibuchi K., Grosse R. The source of Dia1 and IQGAP1 (both enogenous) inferred from the cells (HeLa) used. Robert.Grosse@pharma.uni-heidelberg.de Accepted 2013-FEB-19 by ORCHARD 01-March-2013: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 2007 Only protein-protein interactions imex curation Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation. Dia1 and IQGAP1 interact in cell migration and phagocytic cup formation. J. Cell Biol. (0021-9525) 2007 imex curation Brandt D.T., Marion S., Griffiths G., Watanabe T., Kaibuchi K., Grosse R. The source of Dia1 and IQGAP1 (both enogenous) inferred from the cells (HeLa) used. Robert.Grosse@pharma.uni-heidelberg.de 01-March-2013: Contacted by IntAct-Help. Only protein-protein interactions mouse-nih_3t3 Mus musculus Swiss NIH 3t3 embryo fibroblast cells nih_3t3 Mouse Swiss NIH embryo contact inhibited fibroblast cells fluorescence imaging fluorescence microscopy fluorescence imaging immunostaining immunostaining Brandt D.T., Marion S., Griffiths G., Watanabe T., Kaibuchi K., Grosse R. The source of Dia1 and IQGAP1 (both enogenous) inferred from the cells (HeLa) used. Robert.Grosse@pharma.uni-heidelberg.de Accepted 2013-FEB-19 by ORCHARD 01-March-2013: Contacted by IntAct-Help. J. Cell Biol. (0021-9525) 2007 Only protein-protein interactions imex curation iqga1_mouse Ras GTPase-activating-like protein IQGAP1 Iqgap1 protein protein mouse Mus musculus Mouse MSAAEEVDGLGVVRPHYGSVLDNERLTAEEMDERRRQNVAYEYLCHLEEAKRWMEACLGEDLPPTTELEEGLRNGVYLAKLGNFFSPKVVSLKKIYDREQTRYKATGLHFRHTDNVIQWLNAMDEIGLPKIFYPETTDIYDRKNMPRCIYCIHALSLYLFKLGLAPQIQDLYGKVDFTEEEINNMKIELEKYGIQMPAFSKIGGILANELSVDEAALHAAVIAINEAIDRRVAADTFTALKNPNAMLVNLEEGLAPTYQDVLYQAKQDKMTNAKNRTENSDRERDVYEELLTQAEIQGNVNKVNTSSALANISLALEQGCAVTLLKALQSLALGLRGLQTQNSDWYMKQLQSDLQQKRQSGQTDPLQKEEVQAGVDAANSAAQQYQRRLAAVAAINAAIQKGIAEKTVLELMNPEAQLPQVYPFAADLYQKELATLQQQSPEHSLTHPELTVAVEMLSSVALINRALESGDMTTVWKQLSSSVTGLTNIEEENCQRYLDELMKLKAQAHAENNAFITWNDIQACVDHVNLVVHEEHERILAIGLINEALDEGDAQKTLQALQIPAAKLEGVLAEVAQHYQDTLIRAKREKAQETQDESAVLWLDEIQGGIWQSNKDTQEAQRFALGISAINEAVDSGDVGRTLSALRSPDVGLYGVIPECGETYQSDLAEAKKKRLAAGDNNSKWVKHWVKGGYHYYHNLETQAGGWAEPPDFVQNSVQLSREEIQSSISGVTAAYNREQLWLANEGLITKLQACCRGYLVRQEFRSRMNFLKKQIPAITCIQSQWRGYKQKKAYQDRLAYLHSHKDEVVKIQSLARMHQARKRYRDRLQYFRDHINDIIKIQAFIRANKARDDYKTLINAEDPPMIVVRKFVHLLDQSDQDFQEELDLMKMREEVITLIRSNQQLENDLNLMDIKIGLLVKNKITLQDVVSHSKKLTKKNKEQLSDMMMINKQKGGLKALSKEKREKLEAYQHLFYLLQTNPTYLAKLIFQMPQNKSTKFMDSVIFTLYNYASNQREEYLLLRLFQTALQEEIKSKVDQIQEIVTGNPTVIKMVVSFNRGARGQNALRQILAPVVKEIMDDKSLNIKTDPVDIYKSWVNQMESQTGEASKLPYDVTPEQALSHEEVKTRLDNSIRNMRAVTDKFLSAIVSSVDKIPYGMRFIAKVLKDSLHEKFPDAGEDELLKIIGNLLYYRYMNPAIVAPDAFDIIDLSAGGQLTTDQRRNLGSIAKMLQHAASNKMFLGDNAHLSIINEYLSQSYQKFRRFFQVACDVPELQDKFNVDEYSDLVTLTKPVIYISIGEIINTHTLLLDHQDAIAPEHNDPIHELLDDLGEVPTIESLIGESCGNSNDPNKEALAKTEVSLTLTNKFDVPGDENAEMDARTILLNTKRLIVDVIRFQPGETLTEILETPATNEQEAEHQRAMQRRAIRDAKTPDKMKKSKPMKEDNNLSLQEKKEKIQTGLKKLTELGTVDPKNRYQELINDIAKDIRNQRRYRQRRKAELVKLQQTYSALNSKATFYGEQVDYYKSYIKTCLDNLASKGKVSKKPREMKGKKSKKISLKYTAARLHEKGVLLEIEDLQANQFKNVIFEIGPTEEVGDFEVKAKFMGVQMETFMLHYQDLLQLQYEGVAVMKLFDRAKVNVNLLIFLLNKKFYGK 9136695557ADA784 diap1_mouse Protein diaphanous homolog 1 Diaphanous-related formin-1 p140mDIA Diaph1 Diap1 protein protein mouse Mus musculus Mouse MEPSGGGLGPGRGTRDKKKGRSPDELPATGGDGGKHKKFLERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDISDEQVLVLFEQMLVDMNLNEEKQQPLREKDIVIKREMVSQYLHTSKAGMNQKESSRSAMMYIQELRSGLRDMHLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETSGNYDSRNQHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTSIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQELREIENEDMKVQLCVFDEQGDEDFFDLKGRLDDIRMEMDDFGEVFQIILNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECVSQIVLHKNGTDPDFKCRHLQIDIERLVDQMIDKTKVEKSEAKATELEKKLDSELTARHELQVEMKKMENDFEQKLQDLQGEKDALDSEKQQITAQKQDLEAEVSKLTGEVAKLSKELEDAKNEMASLSAVVVAPSVSSSAAVPPAPPLPGDSGTVIPPPPPPPPLPGGVVPPSPPLPPGTCIPPPPPLPGGACIPPPPQLPGSAAIPPPPPLPGVASIPPPPPLPGATAIPPPPPLPGATAIPPPPPLPGGTGIPPPPPPLPGSVGVPPPPPLPGGPGLPPPPPPFPGAPGIPPPPPGMGVPPPPPFGFGVPAAPVLPFGLTPKKVYKPEVQLRRPNWSKFVAEDLSQDCFWTKVKEDRFENNELFAKLTLAFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKEEYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSENFSSLLELTLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSADQKMTLLHFLAELCENDHPEVLKFPDELAHVEKASRVSAENLQKSLDQMKKQIADVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGDYFVFDPKKLSVEEFFMDLHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQVNRKAGCAVTSLLASELTKDDAMAPGPVKVPKKSEGVPTILEEAKELVGRAS DIP protein O08808 original sequence version: 62 DIP protein O08808 original sequence version: 141 09404164873CA7C1 iqga1_human Ras GTPase-activating-like protein IQGAP1 IQGAP1 KIAA0051 p195 protein protein human Homo sapiens Human MSAADEVDGLGVARPHYGSVLDNERLTAEEMDERRRQNVAYEYLCHLEEAKRWMEACLGEDLPPTTELEEGLRNGVYLAKLGNFFSPKVVSLKKIYDREQTRYKATGLHFRHTDNVIQWLNAMDEIGLPKIFYPETTDIYDRKNMPRCIYCIHALSLYLFKLGLAPQIQDLYGKVDFTEEEINNMKTELEKYGIQMPAFSKIGGILANELSVDEAALHAAVIAINEAIDRRIPADTFAALKNPNAMLVNLEEPLASTYQDILYQAKQDKMTNAKNRTENSERERDVYEELLTQAEIQGNINKVNTFSALANIDLALEQGDALALFRALQSPALGLRGLQQQNSDWYLKQLLSDKQQKRQSGQTDPLQKEELQSGVDAANSAAQQYQRRLAAVALINAAIQKGVAEKTVLELMNPEAQLPQVYPFAADLYQKELATLQRQSPEHNLTHPELSVAVEMLSSVALINRALESGDVNTVWKQLSSSVTGLTNIEEENCQRYLDELMKLKAQAHAENNEFITWNDIQACVDHVNLVVQEEHERILAIGLINEALDEGDAQKTLQALQIPAAKLEGVLAEVAQHYQDTLIRAKREKAQEIQDESAVLWLDEIQGGIWQSNKDTQEAQKFALGIFAINEAVESGDVGKTLSALRSPDVGLYGVIPECGETYHSDLAEAKKKKLAVGDNNSKWVKHWVKGGYYYYHNLETQEGGWDEPPNFVQNSMQLSREEIQSSISGVTAAYNREQLWLANEGLITRLQARCRGYLVRQEFRSRMNFLKKQIPAITCIQSQWRGYKQKKAYQDRLAYLRSHKDEVVKIQSLARMHQARKRYRDRLQYFRDHINDIIKIQAFIRANKARDDYKTLINAEDPPMVVVRKFVHLLDQSDQDFQEELDLMKMREEVITLIRSNQQLENDLNLMDIKIGLLVKNKITLQDVVSHSKKLTKKNKEQLSDMMMINKQKGGLKALSKEKREKLEAYQHLFYLLQTNPTYLAKLIFQMPQNKSTKFMDSVIFTLYNYASNQREEYLLLRLFKTALQEEIKSKVDQIQEIVTGNPTVIKMVVSFNRGARGQNALRQILAPVVKEIMDDKSLNIKTDPVDIYKSWVNQMESQTGEASKLPYDVTPEQALAHEEVKTRLDSSIRNMRAVTDKFLSAIVSSVDKIPYGMRFIAKVLKDSLHEKFPDAGEDELLKIIGNLLYYRYMNPAIVAPDAFDIIDLSAGGQLTTDQRRNLGSIAKMLQHAASNKMFLGDNAHLSIINEYLSQSYQKFRRFFQTACDVPELQDKFNVDEYSDLVTLTKPVIYISIGEIINTHTLLLDHQDAIAPEHNDPIHELLDDLGEVPTIESLIGESSGNLNDPNKEALAKTEVSLTLTNKFDVPGDENAEMDARTILLNTKRLIVDVIRFQPGETLTEILETPATSEQEAEHQRAMQRRAIRDAKTPDKMKKSKSVKEDSNLTLQEKKEKIQTGLKKLTELGTVDPKNKYQELINDIARDIRNQRRYRQRRKAELVKLQQTYAALNSKATFYGEQVDYYKSYIKTCLDNLASKGKVSKKPREMKGKKSKKISLKYTAARLHEKGVLLEIEDLQVNQFKNVIFEISPTEEVGDFEVKAKFMGVQMETFMLHYQDLLQLQYEGVAVMKLFDRAKVNVNLLIFLLNKKFYGK DIP protein P46940 original sequence version: 158 DIP protein P46940 original sequence version: 152 A52EC629596EAC33 diap1_human Protein diaphanous homolog 1 DIAPH1 DIAP1 Diaphanous-related formin-1 protein protein human Homo sapiens Human MEPPGGSLGPGRGTRDKKKGRSPDELPSAGGDGGKSKKFTLKRLMADELERFTSMRIKKEKEKPNSAHRNSSASYGDDPTAQSLQDVSDEQVLVLFEQMLLDMNLNEEKQQPLREKDIIIKREMVSQYLYTSKAGMSQKESSKSAMMYIQELRSGLRDMPLLSCLESLRVSLNNNPVSWVQTFGAEGLASLLDILKRLHDEKEETAGSYDSRNKHEIIRCLKAFMNNKFGIKTMLETEEGILLLVRAMDPAVPNMMIDAAKLLSALCILPQPEDMNERVLEAMTERAEMDEVERFQPLLDGLKSGTTIALKVGCLQLINALITPAEELDFRVHIRSELMRLGLHQVLQDLREIENEDMRVQLNVFDEQGEEDSYDLKGRLDDIRMEMDDFNEVFQILLNTVKDSKAEPHFLSILQHLLLVRNDYEARPQYYKLIEECISQIVLHKNGADPDFKCRHLQIEIEGLIDQMIDKTKVEKSEAKAAELEKKLDSELTARHELQVEMKKMESDFEQKLQDLQGEKDALHSEKQQIATEKQDLEAEVSQLTGEVAKLTKELEDAKKEMASLSAAAITVPPSVPSRAPVPPAPPLPGDSGTIIPPPPAPGDSTTPPPPPPPPPPPPPLPGGVCISSPPSLPGGTAISPPPPLSGDATIPPPPPLPEGVGIPSPSSLPGGTAIPPPPPLPGSARIPPPPPPLPGSAGIPPPPPPLPGEAGMPPPPPPLPGGPGIPPPPPFPGGPGIPPPPPGMGMPPPPPFGFGVPAAPVLPFGLTPKKLYKPEVQLRRPNWSKLVAEDLSQDCFWTKVKEDRFENNELFAKLTLTFSAQTKTSKAKKDQEGGEEKKSVQKKKVKELKVLDSKTAQNLSIFLGSFRMPYQEIKNVILEVNEAVLTESMIQNLIKQMPEPEQLKMLSELKDEYDDLAESEQFGVVMGTVPRLRPRLNAILFKLQFSEQVENIKPEIVSVTAACEELRKSESFSNLLEITLLVGNYMNAGSRNAGAFGFNISFLCKLRDTKSTDQKMTLLHFLAELCENDYPDVLKFPDELAHVEKASRVSAENLQKNLDQMKKQISDVERDVQNFPAATDEKDKFVEKMTSFVKDAQEQYNKLRMMHSNMETLYKELGEYFLFDPKKLSVEEFFMDLHNFRNMFLQAVKENQKRRETEEKMRRAKLAKEKAEKERLEKQQKREQLIDMNAEGDETGVMDSLLEALQSGAAFRRKRGPRQANRKAGCAVTSLLASELTKDDAMAAVPAKVSKNSETFPTILEEAKELVGRAS 9C8273DE4748564F diaph1-iqgap1-1 3 13 unspecified role unspecified role bait bait 12 unspecified role unspecified role prey prey physical association physical association Fig 1C. The amount of IQGAP1 coprecipitating with Dia1 increased in serum-stimulated cells. diaph1-iqgap1-3 7 12 unspecified role unspecified role prey prey human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 11 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined fh3 domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association Fig 1A, S1. diaph1-iqgap1-5 5 12 unspecified role unspecified role prey prey human-hela Homo sapiens epitheloid cervix carcinoma cells Human hela Human epitheloid cervix carcinoma HeLa cells derivation: cervix; epithelial; adenocarcinoma ATCC no:CCL-2 11 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined fh3 domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) physical association physical association Fig 1B. diaph1-iqgap1-7 9 11 unspecified role unspecified role neutral component neutral component region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined fh3 domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 10 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 1D, 3D, 3G. iqgap1-diaph1-1 2 12 unspecified role unspecified role bait bait region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminus sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 11 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined fh3 domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain 38 35 physical association physical association Fig 2B. iqgap1-diaph1-2 4 12 unspecified role unspecified role prey prey region gfp tag green fluorescent protein tag GFP green fluorescent protein GFP green fluorescent protein gfp tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 11 unspecified role unspecified role bait bait fh3 domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined armadillo repeats 3,4 required to bind necessary binding region required to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain physical association physical association Fig 2C, 2D, 2E. iqgap1-diaph1-3 6 12 unspecified role unspecified role bait bait dbr region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey region his L-histidine residue (2S)-2-amino-3-(1H-imidazol-4-yl)propanoic acid (2S)-2-amino-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 2-azanyl-3-(1H-imidazol-4-yl)propanoic acid 2-azanyl-3-(1H-imidazol-5-yl)propanoic acid [tautomer] 4-(2-amino-2-carboxyethyl)imidazole L-histidine alpha-amino-beta-(4-imidazole)propionic acid glyoxaline-5-alanine His undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 48 52 direct interaction direct interaction Fig 2F. iqgap1-diaph1-5 1 12 unspecified role unspecified role bait bait dbr region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined n-terminal region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 59 55 direct interaction direct interaction Fig 2G. IQGAP1 did not interact with Dia1 NT region in the presence of the Dia1 CT region (due to autoinhibition between the Dia1 NT and CT regions), but it interacted with Dia NT in the presence of both Dia1 CT and RhoA. diaph1-iqgap1-9 8 11 unspecified role unspecified role neutral component neutral component 10 unspecified role unspecified role neutral component neutral component colocalization colocalization Fig 4D. iqgap1-diaph1-6 2 12 unspecified role unspecified role bait bait dbr region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain region flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined IQGAP1 was Flag-GFP-tagged. 11 unspecified role unspecified role prey prey region myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Suppl. Fig 2B. diaph1-iqgap1-11 1 12 unspecified role unspecified role prey prey region his tag his tag Hexa-His-tag 6-His-tag Histidine-tag Hexa-His-tag Histidine-tag 6-His-tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined c-terminal region sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 11 unspecified role unspecified role bait bait region gst tag glutathione s tranferase tag glutathione S-tranferase tag glutathione S-tranferase tag gst tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined fh3 domain sufficient to bind sufficient binding region sufficient to bind deletion analysis deletion analysis certain certain sequence position certain certain certain sequence position certain ecoli Escherichia coli (strain K12) 75 72 direct interaction direct interaction Suppl. fig 2C.