bhf-ucl Prickle-1 negatively regulates Wnt/beta-catenin pathway by promoting Dishevelled ubiquitination/degradation in liver cancer. Prickle-1 negatively regulates Wnt/beta-catenin pathway by promoting Dishevelled ubiquitination/degradation in liver cancer. Gastroenterology (0016-5085) 2006 imex curation Chan D.W., Chan C.Y., Yam J.W., Ching Y.P., Ng I.O. The species of Dvl3 is not clearly stated in the paper. The antibody that was used to detect Dvl3 recognises mouse, human and rat. The author has been contacted, but there was no response. In the materials and methods section it is stated that the parent construct (pCDNA/Dvl3) was a gift by Dr M. Semenov. A publication by M. Semenow (PMID: 9192851) describing the cloning of human Dvl3 was traced and therefore it was inferred that the species is human. iolng@hkucc.hku.hk 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip anti tag western anti tag western blot anti tag western Chan D.W., Chan C.Y., Yam J.W., Ching Y.P., Ng I.O. The species of Dvl3 is not clearly stated in the paper. The antibody that was used to detect Dvl3 recognises mouse, human and rat. The author has been contacted, but there was no response. In the materials and methods section it is stated that the parent construct (pCDNA/Dvl3) was a gift by Dr M. Semenov. A publication by M. Semenow (PMID: 9192851) describing the cloning of human Dvl3 was traced and therefore it was inferred that the species is human. iolng@hkucc.hku.hk Accepted 2013-NOV-28 by ORCHARD Gastroenterology (0016-5085) 2006 imex curation Prickle-1 negatively regulates Wnt/beta-catenin pathway by promoting Dishevelled ubiquitination/degradation in liver cancer. Prickle-1 negatively regulates Wnt/beta-catenin pathway by promoting Dishevelled ubiquitination/degradation in liver cancer. Gastroenterology (0016-5085) 2006 imex curation Chan D.W., Chan C.Y., Yam J.W., Ching Y.P., Ng I.O. The species of Dvl3 is not clearly stated in the paper. The antibody that was used to detect Dvl3 recognises mouse, human and rat. The author has been contacted, but there was no response. In the materials and methods section it is stated that the parent construct (pCDNA/Dvl3) was a gift by Dr M. Semenov. A publication by M. Semenow (PMID: 9192851) describing the cloning of human Dvl3 was traced and therefore it was inferred that the species is human. iolng@hkucc.hku.hk 13-Jan-2014: Contacted by IntAct-Help. Only protein-protein interactions human-293 Homo sapiens transformed primary embryonal kidney cells Human 293 Transformed human primary embryonal kidney cells. The Cell type named 293 is synonymous with HEK-293. anti tag coip anti tag coimmunoprecipitation anti tag coip western blot western blot Immuno blot Immuno blot Chan D.W., Chan C.Y., Yam J.W., Ching Y.P., Ng I.O. The species of Dvl3 is not clearly stated in the paper. The antibody that was used to detect Dvl3 recognises mouse, human and rat. The author has been contacted, but there was no response. In the materials and methods section it is stated that the parent construct (pCDNA/Dvl3) was a gift by Dr M. Semenov. A publication by M. Semenow (PMID: 9192851) describing the cloning of human Dvl3 was traced and therefore it was inferred that the species is human. iolng@hkucc.hku.hk Accepted 2013-NOV-28 by ORCHARD Gastroenterology (0016-5085) 2006 imex curation dvl3_human Segment polarity protein dishevelled homolog DVL-3 DVL3 KIAA0208 DSH homolog 3 protein protein human Homo sapiens Human MGETKIIYHLDGQETPYLVKLPLPAERVTLADFKGVLQRPSYKFFFKSMDDDFGVVKEEISDDNAKLPCFNGRVVSWLVSAEGSHPDPAPFCADNPSELPPPMERTGGIGDSRPPSFHPHAGGGSQENLDNDTETDSLVSAQRERPRRRDGPEHATRLNGTAKGERRREPGGYDSSSTLMSSELETTSFFDSDEDDSTSRFSSSTEQSSASRLMRRHKRRRRKQKVSRIERSSSFSSITDSTMSLNIITVTLNMEKYNFLGISIVGQSNERGDGGIYIGSIMKGGAVAADGRIEPGDMLLQVNEINFENMSNDDAVRVLREIVHKPGPITLTVAKCWDPSPRGCFTLPRSEPIRPIDPAAWVSHTAAMTGTFPAYGMSPSLSTITSTSSSITSSIPDTERLDDFHLSIHSDMAAIVKAMASPESGLEVRDRMWLKITIPNAFIGSDVVDWLYHNVEGFTDRREARKYASNLLKAGFIRHTVNKITFSEQCYYIFGDLCGNMANLSLHDHDGSSGASDQDTLAPLPHPGAAPWPMAFPYQYPPPPHPYNPHPGFPELGYSYGGGSASSQHSEGSRSSGSNRSGSDRRKEKDPKAGDSKSGGSGSESDHTTRSSLRGPRERAPSERSGPAASEHSHRSHHSLASSLRSHHTHPSYGPPGVPPLYGPPMLMMPPPPAAMGPPGAPPGRDLASVPPELTASRQSFRMAMGNPSEFFVDVM DIP protein Q92997 original sequence version: 110 B1A55EBF9507D06E pric1_human Prickle-like protein 1 PRICKLE1 RILP REST/NRSF-interacting LIM domain protein 1 protein protein human Homo sapiens Human MPLEMEPKMSKLAFGCQRSSTSDDDSGCALEEYAWVPPGLRPEQIQLYFACLPEEKVPYVNSPGEKHRIKQLLYQLPPHDNEVRYCQSLSEEEKKELQVFSAQRKKEALGRGTIKLLSRAVMHAVCEQCGLKINGGEVAVFASRAGPGVCWHPSCFVCFTCNELLVDLIYFYQDGKIHCGRHHAELLKPRCSACDEIIFADECTEAEGRHWHMKHFCCLECETVLGGQRYIMKDGRPFCCGCFESLYAEYCETCGEHIGVDHAQMTYDGQHWHATEACFSCAQCKASLLGCPFLPKQGQIYCSKTCSLGEDVHASDSSDSAFQSARSRDSRRSVRMGKSSRSADQCRQSLLLSPALNYKFPGLSGNADDTLSRKLDDLSLSRQGTSFASEEFWKGRVEQETPEDPEEWADHEDYMTQLLLKFGDKSLFQPQPNEMDIRASEHWISDNMVKSKTELKQNNQSLASKKYQSDMYWAQSQDGLGDSAYGSHPGPASSRRLQELELDHGASGYNHDETQWYEDSLECLSDLKPEQSVRDSMDSLALSNITGASVDGENKPRPSLYSLQNFEEMETEDCEKMSNMGTLNSSMLHRSAESLKSLSSELCPEKILPEEKPVHLPVLRRSKSQSRPQQVKFSDDVIDNGNYDIEIRQPPMSERTRRRVYNFEERGSRSHHHRRRRSRKSRSDNALNLVTERKYSPKDRLRLYTPDNYEKFIQNKSAREIQAYIQNADLYGQYAHATSDYGLQNPGMNRFLGLYGEDDDSWCSSSSSSSDSEEEGYFLGQPIPQPRPQRFAYYTDDLSSPPSALPTPQFGQRTTKSKKKKGHKGKNCIIS 753D68BD5A4D0935 prickle1-dvl3 2 3 unspecified role unspecified role prey prey tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role bait bait tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 3A prickle1-dvl3-1 1 4 unspecified role unspecified role bait bait tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 3 unspecified role unspecified role prey prey tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 4B dvl3-prickle1 2 3 unspecified role unspecified role bait bait tag flag tag flag tag FLAG-tagged FLAG DYKDDDDKV epitope tag DYKDDDDKV epitope tag FLAG FLAG-tagged undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined 4 unspecified role unspecified role prey prey tag myc tag myc tag EUKLISEED epitope tag EUKLISEED epitope tag undetermined undetermined sequence position undetermined undetermined undetermined sequence position undetermined physical association physical association Fig. 4B, C