IntAct European Bioinformatics Institute IntAct intact http://www.ebi.ac.uk/ http://www.ebi.ac.uk/intact/query/${ac} EBI-[0-9]+|IA:[0-9]+ INTerAction database (IntAct) provides an open source database and toolkit for the storage, presentation and analysis of molecular interactions. http://www.ebi.ac.uk/intact European Bioinformatics Institute; Wellcome Trust Genome Campus; Hinxton, Cambridge; CB10 1SD; United Kingdom http://www.ebi.ac.uk/intact/ Structure of the Escherichia coli ThiS-ThiF complex, a key component of the sulfur transfer system in thiamin biosynthesis. Structure of the Escherichia coli ThiS-ThiF complex, a key component of the sulfur transfer system in thiamin biosynthesis. Biochemistry (0006-2960) 2006 imex curation Lehmann C., Begley T.P., Ealick S.E. see3@cornell.edu,tbp2@cornell.edu 22-Nov-2013: Contacted by IntAct-Help. Only protein-protein interactions in vitro In vitro x-ray diffraction x-ray crystallography X-ray X-ray x-ray diffraction predetermined predetermined participant predetermined Lehmann C., Begley T.P., Ealick S.E. see3@cornell.edu,tbp2@cornell.edu Accepted 2013-NOV-19 by MGT 22-Nov-2013: Contacted by IntAct-Help. Biochemistry (0006-2960) 2006 Only protein-protein interactions imex curation this_ecoli Sulfur carrier protein ThiS thiS thiG1 Thiamine biosynthesis protein ThiS b4407 JW3955 protein protein ecoli Escherichia coli (strain K12) MQILFNDQAMQCAAGQTVHELLEQLDQRQAGAALAINQQIVPREQWAQHIVQDGDQILLFQVIAGG 9AB3C87835758B40 thif_ecoli Sulfur carrier protein ThiS adenylyltransferase thiF b3992 JW3956 protein protein ecoli Escherichia coli (strain K12) MNDRDFMRYSRQILLDDIALDGQQKLLDSQVLIIGLGGLGTPAALYLAGAGVGTLVLADDDDVHLSNLQRQILFTTEDIDRPKSQVSQQRLTQLNPDIQLTALQQRLTGEALKDAVARADVVLDCTDNMATRQEINAACVALNTPLITASAVGFGGQLMVLTPPWEQGCYRCLWPDNQEPERNCRTAGVVGPVVGVMGTLQALEAIKLLSGIETPAGELRLFDGKSSQWRSLALRRASGCPVCGGSNADPV 9CB9E20FD094F12D thif-this 1 2 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) 3 unspecified role unspecified role neutral component neutral component ecoli Escherichia coli (strain K12) direct interaction direct interaction TABLE 2 Rwork = 0.17, Rfree = 0.23 1.98 A